scholarly journals In vivo effect of the aqueous extract of Adansonia digitata (Linn) fruit pulp on Trypanosoma brucei brucei infection in Wistar rats

2020 ◽  
Vol 17 (4) ◽  
pp. 1-8
Author(s):  
O.O. Ogunleye ◽  
I.D. Jatau ◽  
A.J. Natala ◽  
CO Idehen ◽  
O. Salami ◽  
...  
Keyword(s):  
Body Weight ◽  
Trypanosoma Brucei ◽  
Aqueous Extract ◽  
Albino Rats ◽  
Fruit Pulp ◽  
Adansonia Digitata ◽  
Trypanosoma Brucei Brucei ◽  
Significant Difference ◽  
Group A

This study investigated the effects of aqueous extract of fruit pulp of Adansonia digitata on albino rats infected with Trypanosoma brucei brucei. Acute toxicity test was conducted on the extract and then analysed for some phytoconstituents. Thirty-five adult rats were divided into seven groups of five rats each. Group A were the non-infected control group while groups B, C, D, E, F and G were inoculated with 1x 106 trypanosomes per 100 g body weight (BW). At day 6 post infection (6 PI), groups C and D were treated with diminazene aceturate and vitamin C at dose rates of 3.5 mg/kg BW intra peritonealy once and 200 mg/kg BW orally for 3 days respectively, while groups E, F, G were orally treated for 3 days with 40, 80, and 160 mg/kg BW of the extract respectively. The rats were monitored for parasitaemia, PCV and body weight. The LD50 of the extract was greater than 9000 mg/kg. The phytochemical analysis revealed 3.51% flavonoid, 0.07% alkaloid, 0.10% saponin and 0.03.0% oxalate in the extract. While there was progressive increase in parasitaemia from day 6 PI in groups E and F throughout the study, parasitaemia decreases and was completely cleared by day 8 and 11 PI in groups C and G respectively. PCV of group A was not significantly different (p>0.05) from that of F and G. There was significant difference (p<0.05) in the PCV of Group A and that of groups B and C and highly significantly different (p<0.01) with that of D and E. Significant (p<0.05) body weight increase of rats in groups D, E, F and G at day 10 PI was observed. Thus, fruit pulp of A. digitata at a dose of 9000 mg/kg was not toxic to rats, and contains active compounds with potential In vivo anti-trypanosoma activity. Keywords: Adansonia digitata, Albino rats, Animal trypanosomosis, Phytochemicals, Trypanosoma brucei brucei

scholarly journals In vitro and in vivo anti-trypanosomal potentials of Afrormosia laxiflora and Khaya senegalensis against Trypanosoma brucei brucei

2018 ◽  
Vol 39 (3) ◽  
pp. 269-284
Author(s):  
G.D. Chechet ◽  
J Yahaya ◽  
A.J. Nok
Keyword(s):  
Body Weight ◽  
Trypanosoma Brucei ◽  
Post Infection ◽  
Methanol Extract ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Trypanosoma Brucei Brucei ◽  
Khaya Senegalensis

Animal African trypanosomiasis (AAT) also known as Nagana is a resurgent disease in Africa. Medicinal plants are being used in less developed countries for the treatment of various diseases including trypanosomiasis, due to the high cost of currently available drugs. Most of these plants have been useful sources of treatment of various diseases based on information obtained from folk medicine but have not been scientifically certified. Here, we investigated the in vitro and in vivo anti-trypanosomal potentials of the methanol extract of Aformorsia laxiflora and Khaya senegalensis against T. b. brucei. Phytochemical screening as well as LD50 of the plant extracts was carried out following standard procedures. Parasitemia was monitored daily while Packed Cell Volume was determined at three time points (days 1, 4 and 7) during the course of the infection. The phytochemical analysis showed the presence of saponins, alkaloids, flavonoids, antraquinones, resins and tanins. However, steriods/terpenoids were absent in K. senegalensis but present in A. laxiflora. The toxicity of methanol extract of both A. laxiflora and K. senegalensis was above 5000mg/kg body weight. Methanol extracts of A. laxiflora (leaves) and K. senegalensis (stem bark) showed promising trypanocidal potential in vitro against T. b. brucei at concentrations of 10, 15, 25mg/ml and 40 and 20mg/ml respectively. At these concentrations, both extracts immobilized the parasites within 55mins post-incubation. In general, A. laxiflora leaf extract demonstrated prophylactic activity against T. b. brucei in vivo at a dose of 500mg/Kg body weight particularly in group C animals where a delayed pre-patent period (6 days post-infection), extended survival (14 days post-infection) and significant (P<0.05) reduction in the parasite burden confirmed by an absence of anemia (PCV 47.00±0.8 %) was observed when compared to the infected untreated control group. K. senegalensis extract on the other hand did not show anti-trypanosomal activity in the treated groups (1, 2, and 3). Based on these observations, it was therefore deduced that the methanol extract of leaves of A. laxiflora possessed the ability to ameliorate the burden of the disease and could be a plausible candidate for drug development against the disease.Keywords: Trypanosoma brucei brucei, Afromosia laxiflora, Khaya senegalensis, anti-trypanosomal, in vitro, in vivo

Chemical composition analysis and in vivoAnti-diabetic activity of aqueous extract of aerial part of Pallenis spinosa in diabetic rats

2021 ◽  
Vol 17 ◽  
Author(s):  
Abdelkrim Khettaf ◽  
Seloua Dridi
Keyword(s):  
Body Weight ◽  
Chemical Analysis ◽  
Aqueous Extract ◽  
Aerial Part ◽  
Control Group ◽  
Albino Rats ◽  
Spectrometric Analysis ◽  
Composition Analysis ◽  
Significant Activity

Background: The aim of this work is to confirm the anti-diabetic activity of Pallenis spinosa as recommended in traditional medicine and the chemical analysis of the aerial part of Pallenis spinosa. Methods: The isolated compounds have been identified by the chemical methods and spectrometric analysis such as: UV, 1H NMR, and 13C NMR spectroscopy. With regard to anti-diabetic activity, a series of experiments have been carried out in vivo on Westar albino rats. Diabetes has been induced in the animals by an intraperitoneal injection of streptozotocine; they have been treated a day with an aqueous extract from the aerial part infusion (250 and 500 mg/kg body weight) and glibenclamide (5 mg/kg body weight) for 21 days. Results: The chemical analysis of the aerial part of Pallenis spinosa has led to the isolation of five known flavanoid: Patuletin 7-galactopyranoside, patuletin-3-O-α-L-rharnnopyranosyl (1-6)-β-D-galactopyranoside, tricin 7-glucopyranoside, tricin and quercetin. The aqueous extract of both doses 250 mg and 500 mg has shown a significant activity in reducing blood sugar with 43.38% for the dose of 250 mg / kg and 37.76% for the dose of 500 mg / kg, as well as a significant decrease in the total fatness, triglycerides, and the total cholesterol levels in animals Treatment compared to diabetes control group (P = 0.05). We’ve used Glibenclamide as a reference, and it has shown similar results. Conclusion: In order to explore and develop new anti-diabetes drugs, more studies are needed on this plant for scrutinizing its mechanism of activity.

scholarly journals Evaluation of the Efficacy of a Combination of Artemether and ‎Diminazene Aceturate Therapy in Experimental Trypanosoma brucei ‎Infection in Rats

2020 ◽  
Vol 17 (2) ◽  
pp. 7-12
Author(s):  
E. W. Okonkwo ◽  
B. M. Anene ◽  
J. I. Ihedioha
Keyword(s):  
Body Weight ◽  
Trypanosoma Brucei ◽  
Post Infection ◽  
Standard Dose ◽  
Experimental Period ◽  
Albino Rats ◽  
Diminazene Aceturate ◽  
Group A ◽  
Effective Treatment Regimen ◽  
Group B

This study evaluated the efficacy of a combination of artemether and diminazene aceturate therapy in experimental Trypanosoma brucei infection in rats. Thirty five male albino rats used for the study were randomly assigned to seven groups of five rats each as follows: Group A- infected and treated with diminazene aceturate (DA) at 7.0 mg/kg body weight (bw), intramuscular (IM) once on day 7 post-infection (pi); Group B – infected and treated with artemether (ART) at 3.2 mg/kg bw IM on day 7pi and 1.6mg/kg bw IM on days 8,9,10 and 11pi; Groups C,D and E, – infected and treated with DA at 7.0 mg/kg bw IM, 3.5 mg/kg bw IM, and 1.75 mg/kg bw IM respectively, once on day 7pi and ART  at 3.2 mg/kg bw IM on day 7pi plus 1.6mg/kg bw on days 8,9,10 and 11pi; Group F –infected, untreated and Group G – uninfected, untreated. Onset of parasitaemia (OP), level of parasitaemia (LOP), clearance of parasites post treatment, mortality post infection, relapse of parasitaemia post clearance, rectal temperature, and body weight, were determined at specified intervals during the 70-day experimental period. Results showed that there were no significant (p>0.05) variations in the OP and LOP between the infected groups. Trypanosomes were cleared from the blood of rats in group A, C, D, and E after treatment. All the rats in groups B and F were dead by day 14pi. The infection relapsed in groups C and E.  It was concluded that a combination of DA (3.5mg/kg bw once) and ART (3.2 mg/kg bw on day 1 of treatment and 1.6 mg/kg bw for 4 consecutive days) exhibited efficacy comparable to the standard dose of DA at 7mg/kg in the treatment of trypanosome brucei in rats and could thus possibly constitute an effective treatment regimen to reduce the dose of DA and avoid toxicity.  

In vitro and in vivo toxicity assessment of biologically synthesized silver nanoparticles from Elaeodendron croceum

2019 ◽  
Vol 16 (3) ◽  
Author(s):  
S. W. Odeyemi ◽  
J. De La Mare ◽  
A. L. Edkins ◽  
A. J. Afolayan
Keyword(s):  
Silver Nanoparticles ◽  
Body Weight ◽  
Aqueous Extract ◽  
Hematological Parameters ◽  
Weight Ratio ◽  
Control Group ◽  
Significant Difference ◽  
Glutamyl Transferase

Abstract Background The cytotoxic properties of nanoparticles have attracted a great deal of attention in the field of nanoscience and nanotechnology due to their small size and ability to penetrate cellular membranes. Methods The silver nanoparticles were synthesized using Elaeodendron croceum stem bark and characterized. The oral acute toxicity studies were carried out by administration of 500, 1000, 2000 mg/kg body weight to Wister rats in respective groups. An in vitro cytotoxicity assay was evaluated in MDA-MB-231 breast cancer cells using the WST-1 Cell Proliferation assay. The percentage of cell viability after treatment with aqueous extracts of Elaeodendron croceum (ECE) and Elaeodendron croceum silver nanoparticles (ECAgNPs) was compared with that of paclitaxel. Results The in vivo studies revealed that the LD50 was higher than 2000 mg/kg and there was no significant difference (p>0.05) between the treatment groups compared with the control group for mean organ-to-body weight ratio except in the liver and in all hematological parameters except WBC and hematocrit. Similarly, there was no significant difference (p>0.05) for serum electrolytes (Na+, Mg2+ K+, Cl−, and Ca2+), total protein, urea, ɣ-glutamyl transferase (GGT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), Alanine aminotransferase (ALT), albumin, total and conjugated bilirubin between the treatment and the control group. However, there were changes in creatinine, urea, and cholesterol. In the in vitro assays, ECE and ECAgNPs showed IC50 values of 70.87±2.99 and 138.8±3.98 µg/mL respectively against MDA-MB-231 cells compared to paclitaxel, which showed an IC50 value of 80 ng/mL. Conclusion The results showed that the LD50 of the ECE and ECAgNPs in Wister rats was determined to be greater than 2000 mg/kg body weight. The aqueous extract also showed more cytotoxic than the ECAgNPs suggesting that the toxic compounds in aqueous extract were involved in the capping of the AgNPs.

scholarly journals PHYTOCHEMISTRY, TOXICITY AND EFFICACY OF CRUDE AQUEOUS EXTRACT OF CARICA PAPAYA LEAF AGAINST TRYPANOSOMA BRUCEI

2016 ◽  
Vol 14 (1) ◽  
pp. 99-102
Author(s):  
A. A. Biu ◽  
L. B. Buratai ◽  
A. A. Ahmed ◽  
I. U. Hambali ◽  
S. I. Ngulde ◽  
...  
Keyword(s):  
Body Weight ◽  
Trypanosoma Brucei ◽  
Aqueous Extract ◽  
Carica Papaya ◽  
Lethal Dose ◽  
Clinical Signs ◽  
Albino Rats ◽  
Loss Of Appetite ◽  
Crude Aqueous Extract

The aqueous extract of Carica papaya leaf was evaluated for its phytochemistry, acute toxicity and in vitro antitrypanosomal efficacy in this study. A total of 15 albino rats grouped into 5(A-E) of 3 rats each were intraperitoneally treated with graded doses of 100, 200, 400, 800mg/kg body weight of the aqueous extract and observed for 24 hours for clinical signs and death. The calculated median lethal dose (LD50) was 600mg/kg body weight, with clinical signs of sluggishness, awkward pasture, loss of appetite, starry hair coat and terminal death within 24 hours. Severity of clinical signs varied with increasing doses. The in vitro antitrypanosomal efficacy of the aqueous extract showed 100% inhibition of Trypanosoma brucei at concentrations of 40mg/ml, 20mg/ml, 10mg/ml, 5mg/ml and 2.5mg/ml respectively. In conclusion the aqueous extract of C. papaya leaf has bioactive components that are moderately toxic and has trypanosomal inhibitory activity.

scholarly journals Effects Of Ginger Extract On Glomerular Mesangial Matrix Of Kidneys In Alloxan Induced Diabetic Nephropathy Of Albino Rats

2018 ◽  
Vol 08 (02) ◽  
pp. 87-91
Author(s):  
Faiza Irshad ◽  
Saira Munawar ◽  
Areej Rasheed
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Diabetic Nephropathy ◽  
Aqueous Extract ◽  
Control Group ◽  
Albino Rats ◽  
Mesangial Matrix ◽  
Significant Difference ◽  
Group B ◽  
Experimental Group

Background: For a long time, Diabetes mellitus has been treated with medicines derived from plants. Objective: To evaluate the effect of Ginger aqueous extract on Glomerular mesangial matrix in Alloxan induced diabetic nephropathy of albino rats. Materials and Methods: In this study we induced diabetes mellitus with Alloxan intraperitoneally (150 mg/kg body weight) in Experimental groups B & C. Then the rats of Experimental group C received 200mg/kg body weight of ginger aqueous extract by gavage daily for five weeks starting from 8th day after Alloxan injection. Results: We observed that on histopathological examination, Experimental group B kidneys revealed highly increased mesangial matrix while the animals of experimental group C treated with ginger aqueous extract showed less increase in mesangial matrix as compared to experimental group B but it was more than control group A. Three groups had significant difference among them having p-values <0.001. Conclusion: The results of the present study indicated that the co-treatment of Ginger aqueous extract prevented alloxan induced diabetic nephropathy in albino rats. The aqueous extract of Ginger showed amazing results regarding renal histopathology of diabetic rats. The overall nephroprotective effect of Ginger is probably due to a counteraction of free radicals by its antioxidant components

scholarly journals Evaluation of the anti-diabetic potential of aqueous extract of Clerodendrum infortunatum L. in vivo in streptozotocin-induced diabetic Wistar rats

2019 ◽  
Vol 6 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Jyothi S Pillai ◽  
R Ratheesh ◽  
K G Padmakumaran Nair ◽  
M G Sanalkumar ◽  
Robin J Thomson
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Modern Medicine ◽  
Diabetic Rats ◽  
The Body ◽  
Albino Rats ◽  
The Metabolic Syndrome ◽  
Significant Amelioration ◽  
Wistar Albino Rats

Diabetes Mellitus, the metabolic syndrome where the body either fails to produce or effectively utilize insulin, is associated with chronic morbidity. While a definitive cure for the disease is lacking, with the modern medicine offering mainly the means to control the extent of the disease, Complementary and Alternative Medicine (CAMs) offers additional/alternate means to tackle the disease. On the other hand, the lack of evidenced medical practices is a lacuna in most of the traditional medical applications. Clerodendrum infortunatum.L (Lamiaceae family), a perennial shrub found in the tropics, has been known for its numerous pharmacological properties and is found as a constituent in many Ayurvedic and Siddha drugs, especially for skin and respiratory ailments. The plant has a noted potential as anti-hyperglycemic and has been found to be used in traditional medicine for the treatment of diabetes. However, evidence based evaluations have not been conducted on the anti-hyperglycemic effect of the plant, especially with respect to the general mode of intake, i.e, the aqueous form. In the current study, the aqueous extract of C. infortunatum (CI), was scientifically assessed for its effect on streptozotocin induced diabetes in Wistar albino rats. The diabetic rats were divided into 5 groups of 6 animals each. For testing the efficacy of extracts, two groups were intra-orally provided with dosages of 200 mg/Kg and 400 mg/Kg of body weight of animals, respectively, of aqueous extracts of CI. Control groups were maintained for evaluation, which included vehicle control as well as with Glibenclamide, a standard anti-diabetic drug. The extracts at a dose of 400 mg/Kg body weight was found to be associated with significant amelioration of many of the diabetes induced conditions, suggesting that the plant extract could be a strong potential CAM candidate for therapeutic management of diabetes.

scholarly journals Antidiabetic and antiulcerative potential of Garcinia lanceifolia Roxb. bark

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Nilutpal Sharma Bora ◽  
Partha Sarathi Bairy ◽  
Abdus Salam ◽  
Bibhuti Bhusan Kakoti
Keyword(s):  
Body Weight ◽  
Serum Levels ◽  
Northeast India ◽  
Scientific Data ◽  
Antidiabetic Activity ◽  
Histological Evaluation ◽  
Albino Rats ◽  
Antiulcer Activity ◽  
Dose Dependent

Abstract Background Garcinia lanceifolia Roxb. has been used by many ethnic communities of Northeast India to mitigate various disorders like dyspepsia, ulcers, diabetes, etc. However, a robust scientific study on its antidiabetic and antiulcer potential is unavailable till date. The aim of this present study is to scientifically validate if the antidiabetic and antiulcer effects reported by the ethnic tribes of Assam has any scientific value or not. The effects were tested in adult Wistar albino rats using approved animal models for preclinical testing of pharmacological activities. Results The hydroalcoholic extract of the bark of Garcinia lanceifolia Roxb. was prepared and its LD50 was calculated. The LD50 was determined to be greater than 5000 mg/kg body weight. The extract at doses of 250 mg/kg body weight and 500 mg/kg body weight was found to exhibit a very potent dose-dependent antidiabetic activity. The results were backed by a battery of test including analysis of serum levels of blood glucose, lipid profiles, in vivo antioxidant enzymes, and histopathological studies. Evidence of dose-dependent antiulcer activity of the extract was backed by robust scientific data. It was found that HAEGL induced a significant dose-dependent increase in the ulcer index in both alcohol-induced and acetic acid-induced ulcer models, which was evident from the macroscopic observation of the inner lining of the gastric mucosa and the histological evaluation of the extracted stomach. Conclusion The results suggested that the bark of Garcinia lanceifolia (Roxb.) has significant antidiabetic and antiulcer potential. Further studies with respect to the development herbal dosage forms and its safety evaluation are required.

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