SANITARY CONDITIONS IN TWELVE FLUID MILK PROCESSING PLANTS AS DETERMINED BY USE OF THE RINSE FILTER METHOD1

1973 ◽  
Vol 36 (4) ◽  
pp. 237-241 ◽  
Author(s):  
R. T. Marshall ◽  
R. Appel

Experiments were done in 12 fluid milk processing plants to determine their sanitary condition. Tap water was sterilized by membrane filtration and sodium thiosulfate was added to inactivate residual sanitizer. This water was used to rinse the pasteurized milk storage tank, filler bowls and filled containers. Samples of rinse water were filtered through membrane filters which were incubated on media to produce colonies. Total and coliform counts were made. Storage tanks contributed fewer bacteria to the water than other equipment. About 60% of the first cartons taken from fillers were contaminated with at least 1 bacterium per 100 ml of rinse water and 37% contained at least 1 coliform per 100 ml. The method appears useful to evaluate the sanitary conditions of equipment.

1992 ◽  
Vol 55 (1) ◽  
pp. 38-42 ◽  
Author(s):  
TYH-JENQ REN ◽  
JOSEPH F. FRANK

Air in four commercial fluid milk plants was sampled for microbiological and nonmicrobiological particles over a 4-month period. An Andersen two-stage and Ross-Microban sieve samplers, a Biotest RCS sampler, and a Met-one laser particle counter were used to sample air. Air was sampled two to three times per day in raw milk storage, processing, and filling areas. Viable particle counts per 100 L air obtained with the Andersen sampler were 2.03 ± 0.41 (log10 Mean ± SD), 2.26 ± 0.57, and 2.41 ± 0.70 in raw milk storage, processing, and filling areas, respectively. These levels were significantly (p<0.05) greater than those obtained using the RCS and Ross-Microban samplers. Overall correlations of the Ross-Microban and RCS samplers with the Andersen sampler were r2 = 0.71 and 0.62, respectively. Correlations between Andersen sampler results and number of total particles greater than 0.5 μm were r2 = 0.36 in raw milk storage, 0.15 in the processing area, and 0.18 in the filling area.


2007 ◽  
Vol 70 (10) ◽  
pp. 2354-2364 ◽  
Author(s):  
JASON R. HUCK ◽  
NICOLE H. WOODCOCK ◽  
ROBERT D. RALYEA ◽  
KATHRYN J. BOOR

Psychrotolerant endospore-forming bacteria Bacillus and Paenibacillus spp. are important spoilage organisms in fluid milk. A recently developed rpoB subtyping method was applied to characterize the diversity and phylogenetic relationships among Bacillus and related sporeformers associated with milk processing systems. Milk samples representing the processing continuum from raw milk to pasteurized products were collected from two fluid milk processing plants, held at 6°C uptothe code date that had been established by each processing plant (i.e., either 18 or 21 days), and plated for bacterial enumeration throughout storage. Bacterial colonies selected to represent the visible diversity in colony morphology on enumeration plates were examined further. Among 385 bacterial isolates characterized, 35% were Bacillus spp., and 65% were Paenibacillus spp. A total of 92 rpoB allelic types were identified among these isolates, indicating considerable diversity among endospore-forming spoilage organisms present in fluid milk systems. Of the 92 allelic types identified, 19 were isolated from samples collected from both processing plants. The same rpoB allelic types were frequently identified in paired raw milk and packaged product samples, indicating that Bacillus and Paenibacillus spp. can enter dairy processing systems through raw milk. Certain subtypes were found exclusively in pasteurized samples, including those that were temporally independent, suggesting the possibility of in-plant sources for these spoilage organisms, including through the persistence of selected subtypes in processing plants. Development of effective control strategies for the diverse array of psychrotolerant endospore-forming organisms that currently limit the shelf lives of high-temperature short-time fluid milk products will require comprehensive, integrated efforts along the entire milk processing continuum.


2007 ◽  
Vol 7 (5-6) ◽  
pp. 43-51 ◽  
Author(s):  
Y. Matsui ◽  
T. Aizawa ◽  
M. Suzuki ◽  
Y. Kawase

The musty-earthy taste and odour caused by the presence of geosmin and other compounds in tap water are major causes of consumer complaints. Although ozonation and granular activated carbon (GAC) adsorption have been practiced in water-treatment plants to remove these compounds effectively, two major problems associated with the application of these processes – formation of stringently regulated bromate ions by ozonation and unhygienic invertebrate colonisation of GAC filters – are still to be resolved. This research advanced the process of adsorption by powdered activated carbon (PAC) by reducing its particle size to the submicrometre range for microfiltration pretreatment. Adsorption pretreatment by using this super (S)-PAC removed the geosmin with vastly greater efficiency than by normal PAC. Removal was attained in a much shorter contact time and at a much lower dosage. The S-PAC was also beneficial in attenuating the transmembrane pressure rises that occurred between both physical backwashings and chemical cleanings.


2019 ◽  
Vol 6 (12) ◽  
pp. 251-261
Author(s):  
Md. Alamgir Hossain ◽  
Md. Motaher Hossain ◽  
Md. Golam Rasul ◽  
Mohammad Abu Jafor Bapary

The hygienic and sanitary conditions of majority of the retail fish markets are very poor and unhealthy that may have an impact on fish retailers. The present study was conducted in two retail fish markets located in Kajirbazar and Bondorbazar, in Sadar Sylhet, to assess the hygiene and sanitary condition of fish retailers. Information was collected through questionnaire interview and participatory rural appraisal (PRA) from the retailers and other value chain stakeholders. It was found that about 82% of fish retailers were permanent in these retail markets. The retailers are mainly involved in selling various species of fishes such as carps, catfishes, tilapia, koi, hilsa, shrimp and other small indigenous fishes. The sources of these fishes are mainly of nearby haors, river and ponds. The retailers get fishes from various suppliers and also collected from producers. The income level of fish retailers is relatively low although they are trying to improve their socio-economic conditions. Besides, about 9% of the retailers do not have any formal education whereas 70% and 21% have their education in primary and secondary level, respectively. Although the majority of the retailers were found literate, however, their knowledge on public health was seemed to be very poor. Due to lack of proper knowledge on public health, they are frequently affected by various diseases such as common cold, diarrhea, lesion, fever, skin disease and so on. Particularly, 48% of the fish retailers are affected by the lesion on hands, and 31% and 27% of retailers have been affected by the lesion between fingers or toes, respectively. About 20% of the retailers do not have adequate knowledge on fish quality or benefit of using ice in fish preservation in retail markets. Considering all together, the present study revealed that the retailers operated fish markets are ill-managed, unhygienic and unsatisfactory. The lack of proper fish preservation facilities, poor water supply and unplanned infrastructures are generally regarded as a noticeable problem in the retail markets. In addition, the lack of retailer’s education, consciousness about fish quality, lack of training on hygienic and sanitary conditions are seemed to be involved in spoiling or deteriorating fish quality before reaching to the consumers. Therefore, the proper education and training on hygienic and sanitary conditions as well as sufficient knowledge on maintaining fish quality is required for retailers involved in fish retailing in the markets. It can be concluded that the improvement of relevant facilities of fish markets and proper supervision by respective authorities can ensure the better public health for retailers and safe fish food for consumers.


Author(s):  
Doaa HAMDY ◽  
Ayman El-BADRY ◽  
Wegdan ABD EL WAHAB

Background: The protozoan Giardia and Cryptosporidium are responsible for most water-borne diseases all over the world. The extent and number of outbreaks of waterborne diseases suggests a significant risk of their potential transmission via drinking water. This study aimed to document the prevalence and viability of Giardia and Cryptosporidium (oo) cysts in tap water samples in Beni-Suef Governorate, Egypt and to detect the predominant Giardia and Cryptosporidium assemblages/species using nested PCR/ Restriction Fragment Length Polymorphism (RFLP) confirmed by further sequencing of positive samples. Methods: A total of 80 tap water samples were collected throughout a year from four big centers and filtered using the membrane filtration method. Samples were stained by Lugol’s iodine, Modified Zeihl-Neelsen (MZN) (to detect prevalence) and trypan blue stain (to detect viability). Nested PCR-RFLP and sequencing were used for molecular characterizations and genotyping of the detected Giardia and Cryptosporidium. Results: Giardia and Cryptosporidium DNA was detected in 20 (25%) and 29 (36.3%) samples respectively, with predominance of Giardia assemblage B (85%) and C. hominis (75.9%). The prevalence and viability of both parasites (oo) cysts showed seasonality which peaked in summer and were greater in Beba center and in rural areas. Conclusion: To our knowledge, no studies have been done in these areas before. The anthroponotic transmission has an important role in giardiasis and crypto­sporidiosis epidemiology in this studied area.


1983 ◽  
Vol 46 (7) ◽  
pp. 572-577 ◽  
Author(s):  
RABIA ZUBERI ◽  
R. B. QADRI ◽  
PIRZADA M. A. SIDDIQUI

Line and finished product samples (668) from two shrimp processing plants located at the Karachi fish harbor were collected and examined bacteriologically. Frozen shrimp samples (100) collected from Plant A had the following bacterial profile: average (geometric) total aerobic plate count 9.4 × 106 CFU/g; MPN coliforms 94/g; and MPN fecal coliforms 41/g. The bacterial profile of 114 frozen shrimp samples from Plant B were 8.3 × 106 CFU/g, 180/g and 133/g for total plate count, MPN coliform and MPN fecal coliforms, respectively. Samples (126) of raw and laboratory-processed (simulating commercial processing) shrimp were also examined. Processing conditions of Plant A were better and resulted in a better quality end-product as compared to that of Plant B. Three of 338 samples from Plant B yielded Salmonella. The composition of microbial flora was nearly the same for both species of shrimps from both plants. Pseudomonads were the predominant microflora followed by Micrococcus sp. When shrimp were handled expeditiously under good sanitary conditions in the laboratory, the bacterial counts were significantly lower than on shrimp from the plants. Laboratory-processed shrimp had an average (geometric) total aerobic plate count of 8.1 × 105 CFU/g and average (geometric) coliform and fecal coliform MPNs were <3/g.


1982 ◽  
Vol 88 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Amal E. M. Khairy ◽  
Olfat El Sebaie ◽  
Aleya Abdel Gawad ◽  
Laila El Attar

Drinking water is a recognized mode of transmission of various parasitic and bacterial infections and high prevalence rates of water-borne infection have been found during past studies in rural areas of the Nile Delta (Farag et al. 1979; Khairy. Barakat & Omar, 1978).


1970 ◽  
Vol 52 (3) ◽  
pp. 395-402 ◽  
Author(s):  
James W. Gruebele ◽  
Sheldon W. Williams ◽  
Richard F. Fallert

2014 ◽  
Vol 77 (11) ◽  
pp. 1868-1875 ◽  
Author(s):  
HAIQIANG WANG ◽  
ELLIOT T. RYSER

Chemical sanitizers are usually added to dump tank water to minimize cross-contamination during tomato packing. However, the efficacy of sanitizers continues to be questioned. This study assessed the ability of six commonly used sanitizers (40 ppm of peroxyacetic acid, 40 ppm of mixed peracid, 40 ppm of available chlorine alone or acidified to pH 6.0 with citric acid or T-128, and electrolyzed water containing 40 ppm of available chlorine at pH 6.7) to reduce Salmonella on tomatoes, in wash water, and on equipment surfaces using a pilot-scale processing line. Red round tomatoes (11.3 kg) were dip inoculated to contain Salmonella at ~ 6 log CFU/g, air dried for 2 h, treated for 2 min in a 3.3-m-long dump tank and then dried on a roller conveyor, with sanitizer-free water serving as the control. Tomato and water samples were collected at 15-s intervals during washing with additional dump tank, water tank, and roller conveyor surface samples collected after washing. All samples were appropriately neutralized, diluted, and surface plated on Trypticase soy agar containing 0.6% yeast extract, 0.05% ferric ammonium citrate, and 0.03% sodium thiosulfate with or without membrane filtration to enumerate Salmonella. All six sanitizer treatments were more efficacious than the water control (P ≤ 0.05), with chlorine plus citric acid yielding the greatest Salmonella reduction on tomatoes (3.1 log CFU/g). After processing, all sanitizer wash solutions contained significantly lower (P ≤ 0.05) levels of Salmonella than the water control (3.0 log CFU/ml). The four chlorine-based sanitizer treatments yielded significantly lower Salmonella populations (P ≤ 0.05) in the wash solution compared with peroxyacetic acid and mixed peracid. After processing with sanitizers, Salmonella populations decreased to nondetectable levels (< 0.2 log CFU/100 cm2) on the equipment surfaces.


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