Photo-induced antifungal activity of chitosan composite film solution with Nano TiO2 and Nano Ag

2022 ◽  
Author(s):  
yage xing ◽  
Jing Tang ◽  
Xuanlin Li ◽  
Ruihan Huang ◽  
Lin Wu ◽  
...  
Keyword(s):  
Composite Film ◽  
Culture Medium ◽  
Uv Light ◽  
Physiological Mechanism ◽  
Storage Period ◽  
Growth Period ◽  
Intracellular Protein ◽  
Nano Tio2 ◽  
Colony Diameter ◽  
Chitosan Composite

This study investigated the ultraviolet (UV) light-induced effect of chitosan-titanium dioxide-silver (CTS-TiO2-Ag) nanocomposite film solution against Penicillium steckii ( ( P. steckii ) , as well as the underlying the physiological mechanism. The results indicated that the longer the UV exposure time, the better the pathogenic inhibition effect. After UV photoinduced treatment for 120 min, the colony diameter of P. steckii was the smallest at 4.85 mm. However, when this process is followed by an 8-h storage period, the conductivity of the P. steckii culture medium reached its highest level at 713 μs/cm. After a 120 h growth period in the same conditions, the lesion diameters and pathogenicity of the mangoes reached 12.61 mm and 41.67%, respectively. Since the cell membrane was severely disrupted, its permeability increased, causing serious intracellular protein and nucleic acid material extravasation. Furthermore, the malondialdehyde (MDA) , catalase (CAT) and superoxide dismutase (SOD) in the   P. steckii reached maximum levels after 8 h of incubation, at 2.1106 μmol/mL, 44.06 U/mL, and 24.67 U/mL respectively. These results indicated significant P. steckii inhibition via the UV light induction of the CTS-TiO 2 -Ag composite film solution.

scholarly journals Functional Properties of Antimicrobial Neem Leaves Extract Based Macroalgae Biofilms for Potential Use as Active Dry Packaging Applications

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1664
Author(s):  
A. A. Oyekanmi ◽  
U. Seeta Uthaya Kumar ◽  
Abdul Khalil H. P. S. ◽  
N. G. Olaiya ◽  
A. A. Amirul ◽  
...  
Keyword(s):  
Composite Film ◽  
Functional Properties ◽  
Storage Stability ◽  
Storage Period ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Ambient Conditions ◽  
Neem Extract ◽  
Control Film ◽  
Neem Leaves

Antimicrobial irradiated seaweed–neem biocomposite films were synthesized in this study. The storage functional properties of the films were investigated. Characterization of the prepared films was conducted using SEM, FT-IR, contact angle, and antimicrobial test. The macroscopic and microscopic including the analysis of the functional group and the gas chromatography-mass spectrometry test revealed the main active constituents present in the neem extract, which was used an essential component of the fabricated films. Neem leaves’ extracts with 5% w/w concentration were incorporated into the matrix of seaweed biopolymer and the seaweed–neem bio-composite film were irradiated with different dosages of gamma radiation (0.5, 1, 1.5, and 2 kGy). The tensile, thermal, and the antimicrobial properties of the films were studied. The results revealed that the irradiated films exhibited improved functional properties compared to the control film at 1.5 kGy radiation dosage. The tensile strength, tensile modulus, and toughness exhibited by the films increased, while the elongation of the irradiated bio-composite film decreased compared to the control film. The morphology of the irradiated films demonstrated a smoother surface compared to the control and provided surface intermolecular interaction of the neem–seaweed matrix. The film indicated an optimum storage stability under ambient conditions and demonstrated no significant changes in the visual appearance. However, an increase in the moisture content was exhibited by the film, and the hydrophobic properties was retained until nine months of the storage period. The study of the films antimicrobial activities against Staphylococcus aureus (SA), and Bacillus subtilis (BS) indicated improved resistance to bacterial activities after the incorporation of neem leaves extract and gamma irradiation. The fabricated irradiated seaweed–neem bio-composite film could be used as an excellent sustainable packaging material due to its effective storage stability.

scholarly journals TiO2-Powdered Activated Carbon (TiO2/PAC) for Removal and Photocatalytic Properties of 2-Methylisoborneol (2-MIB) in Water

Water ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 1622
Author(s):  
Xiao-Pin Guo ◽  
Peng Zang ◽  
Yong-Mei Li ◽  
Dong-Su Bi
Keyword(s):  
Activated Carbon ◽  
Natural Organic Matter ◽  
Uv Light ◽  
Sol Gel ◽  
Light Irradiation ◽  
Nano Tio2 ◽  
Uv Light Irradiation ◽  
Adsorption Capacities ◽  
Taste And Odor ◽  
Composite Photocatalysts

2-methylisoborneol (2-MIB) is a common taste and odor compound caused by off-flavor secondary metabolites, which represents one of the greatest challenges for drinking water utilities worldwide. A TiO2-coated activated carbon (TiO2/PAC) has been synthesized using the sol-gel method. A new TiO2/PAC photocatalyst has been successfully employed in photodegradation of 2-MIB under UV light irradiation. In addition, the combined results of XRD, SEM-EDX, FTIR and UV-Vis suggested that the nano-TiO2 had been successfully loaded on the surface of PAC. Experimental results of 2-MIB removal indicated that the adsorption capacities of PAC for 2-MIB were higher than that of TiO2/PAC. However, in the natural organic matter (NOM) bearing water, the removal efficiency of 2-MIB by TiO2/PAC and PAC were 97.8% and 65.4%, respectively, under UV light irradiation. Moreover, it was shown that the presence of NOMs had a distinct effect on the removal of MIB by TiO2/PAC and PAC. In addition, a simplified equivalent background compound (SEBC) model could not only be used to describe the competitive adsorption of MIB and NOM, but also represent the photocatalytic process. In comparison to other related studies, there are a few novel composite photocatalysts that could efficiently and rapidly remove MIB by the combination of adsorption and photocatalysis.

scholarly journals Effect of High Pressure Treatment on Poly(lactic acid)/Nano–TiO2 Composite Films

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2621 ◽  
Author(s):  
Hai Chi ◽  
Wenhui Li ◽  
Chunli Fan ◽  
Cheng Zhang ◽  
Lin Li ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
High Pressure ◽  
Lactic Acid ◽  
Composite Film ◽  
Composite Films ◽  
Barrier Properties ◽  
Poly Lactic Acid ◽  
Pressure Treatment ◽  
Nano Tio2 ◽  
High Pressure Treatment

The microstructure, thermal properties, mechanical properties and oxygen and water vapor barrier properties of a poly(lactic acid) (PLA)/nano-TiO2 composite film before and after high pressure treatment were studied. Structural analysis showed that the functional group structure of the high pressure treated composite film did not change. It was found that the high pressure treatment did not form new chemical bonds between the nanoparticles and the PLA. The micro-section of the composite film after high pressure treatment became very rough, and the structure was depressed. Through the analysis of thermal and mechanical properties, high pressure treatment can not only increase the strength and stiffness of the composite film, but also increase the crystallinity of the composite film. Through the analysis of barrier properties, it is found that the barrier properties of composite films after high pressure treatment were been improved by the applied high pressure treatment.

A nano-porous CeO2/Chitosan composite film as the immobilization matrix for colorectal cancer DNA sequence-selective electrochemical biosensor

Talanta ◽  
2006 ◽  
Vol 70 (3) ◽  
pp. 561-565 ◽  
Author(s):  
Ke-Jun Feng ◽  
Yun-Hui Yang ◽  
Zhi-Jie Wang ◽  
Jian-Hui Jiang ◽  
Guo-Li Shen ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Composite Film ◽  
Dna Sequence ◽  
Electrochemical Biosensor ◽  
Immobilization Matrix ◽  
Chitosan Composite

Solid-phase photocatalytic degradation of polyethylene–goethite composite film under UV-light irradiation

2009 ◽  
Vol 172 (2-3) ◽  
pp. 1424-1429 ◽  
Author(s):  
G.L. Liu ◽  
D.W. Zhu ◽  
S.J. Liao ◽  
L.Y. Ren ◽  
J.Z. Cui ◽  
...  
Keyword(s):  
Composite Film ◽  
Photocatalytic Degradation ◽  
Solid Phase ◽  
Uv Light ◽  
Light Irradiation ◽  
Uv Light Irradiation

scholarly journals Selection of the optimal culture medium for cultivation Fusarium sporotrichioides Sherb

2020 ◽  
Author(s):  
I.L. Astapchuk ◽  
◽  
N.A. Marchenko ◽  
G.V. Yakuba ◽  
A.I. Nasonov ◽  
...  
Keyword(s):  
Growth Rate ◽  
Growth And Development ◽  
Maximum Degree ◽  
Culture Medium ◽  
Culture Media ◽  
High Variability ◽  
Fusarium Sporotrichioides ◽  
Cultural Characteristics ◽  
Colony Diameter ◽  
Selection Of

The influence of various culture media on the growth, morphological and cultural characteristics of the fungus F. sporotrichioides was studied. Ten culture media were used in our research. A comparative study of the growth rate of the F. sporotrichioides mycelium made it possible to identify two media that are the most suitable for the cultivation and identification of this species, namely carrot and tomato agar. We took into account such criteria as ensuring the maximum degree of sporulation, rapid growth and development of mycelium (the 7th day), colony diameter (71–78 mm), as well as the ease of preparation. Nirenberg culture medium can be used to obtain a large number of conidia of the fungus. Because of the high variability of cultural characteristics of F. sporotrichioides, we recommend using different composition of media.

scholarly journals Effects of cross-linked dimers of ribonuclease A or of lysozyme on the processing of endocytosed peroxidase by hepatoma cells

1982 ◽  
Vol 202 (2) ◽  
pp. 543-550
Author(s):  
J Bartholeyns ◽  
P Baudhuin
Keyword(s):  
Mechanism Of Action ◽  
Binding Sites ◽  
Culture Medium ◽  
Basic Protein ◽  
Intracellular Localization ◽  
Ribonuclease A ◽  
Protein Processing ◽  
Intracellular Protein ◽  
Intracellular Degradation ◽  
Htc Cells

Cross-linked dimers of ribonuclease, added at a concentration of 0.05 mg/ml to the culture medium of hepatoma (HTC) cells, were previously shown to inhibit intracellular degradation of peroxidase taken up by endocytosis. Intracellular localization showed that endocytosed peroxidase does not reach lysosomes in dimer-treated cells. The present study shows that preloading of lysosomes with fluorescent anti-peroxidase IgG, obtained by exposing HTC cells for 48 h to 0.1 mg of antibody/ml, restores intracellular degradation of endocytosed peroxidase. Moreover, accumulation of peroxidase into lysosomes, which no longer occurs in dimer-treated cells, occurs again under these conditions. We conclude that inhibition of transfer of peroxidase from phagosomes to lysosomes is most likely to be the alteration resulting from the exposure of the cells to ribonuclease dimer, rather than inhibition of fusion between phagosomes and lysosomes. The dimer of another basic protein, lysozyme added at a concentration of 0.2 mg/ml to the culture medium, is shown to induce the same type of effects as does the dimer of ribonuclease; the half-life of endocytosed peroxidase increased from 5 to 15 h after 2 h exposure of HTC cells to dimerized lysozyme. The effect of both dimers on intracellular protein processing can be reversed by addition of 100 mm-galactose to the culture medium, up to 5 h after pretreatment of the cells. The dimers of ribonuclease A or of lysozyme have thus probably the same mechanism of action. Evidence that the two dimers share the same binding sites on the cells is presented.

A Study of the Preparation of Silk Sericin/Chitosan Composite Film for Future Wound Dressing Applications

2015 ◽  
Vol 804 ◽  
pp. 179-182 ◽  
Author(s):  
Piyapong Pankaew ◽  
Pattarinee Klumdoung ◽  
Kittisakchai Naemchanthara
Keyword(s):  
Composite Film ◽  
Wound Dressing ◽  
Composite Films ◽  
Fine Powder ◽  
Silk Sericin ◽  
Vis Spectroscopy ◽  
Silk Worm ◽  
Bombyx Mori Silk ◽  
Film Development ◽  
Chitosan Composite

Silk sericin/chitosan composite films were successfully prepared for possible future wound dressing applications. To prepare the chitosan, shrimp shells were first washed and finely ground to obtain a fine powder before extracting the chitosan using a chemical reaction method. The sericin was extracted from Thai raw cocoons of Bombyx mori silk worm via boiling and drying. To prepare the silk sericin/chitosan composite films, the silk sericin and chitosan solutions with varying volume ratios were mixed in a magnetic stirrer for 30 minutes. They were then dropped on to a plastic mold. The sample was dried at a temperature of 50 °C. The prepared composite films were characterized using UV-VIS spectroscopy, Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM). The results from our studies could provide a method for future composite film development.

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