scholarly journals A Novel HPTLC Method for Simultaneous Quantification of (-)-Pseudosemiglabrin and Isoglabratephrin a Bioactive Prenylated Flavonoids Identified in Tephrosia Apollinea

2020 ◽  
Keyword(s):  
High Performance ◽  
Raw Materials ◽  
Folk Medicine ◽  
Rapid Screening ◽  
Active Components ◽  
Prenylated Flavonoids ◽  
Ethanol Extracts ◽  
Chemical Biomarkers ◽  
Tephrosia Apollinea ◽  
Hptlc Method

Fingerprint analysis by HPTLC is an effective and powerful tool for linking the bioactive components profile of the plants with botanical identity and for estimation of chemical biomarkers. Tephrosia apollinea has been used in folk medicine to treat inflammation and relieving pain, as well as for diarrhea and abdominal pain. A densitometric High Performance Thin Layer Chromatography (HPTLC) fingerprinting method has been developed for the simultaneous determination and quantification of (-)-pseudosemiglabrin (SSG) and isoglabratephrin (IGP) in different parts of Tephrosia apollinea collected in summer and winter. The study revealed that SSG and IGP are the major components in T. apollinea and found in all parts of the plant at different amount. The proposed HPTLC method was found to be convenient for rapid screening of active components present in ethanol extracts of T. apollinea and can be used for analysis and routine quality control of the raw materials as well as formulations containing Tephrosia species

scholarly journals Myrica esculenta Buch.-Ham. ex D. Don: A Natural Source for Health Promotion and Disease Prevention

Plants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 149 ◽  
Author(s):  
Atul Kabra ◽  
Natália Martins ◽  
Rohit Sharma ◽  
Ruchika Kabra ◽  
Uttam Singh Baghel
Keyword(s):  
Health Promotion ◽  
High Performance ◽  
Folk Medicine ◽  
Plant Biotechnology ◽  
Future Research ◽  
Synthetic Seed ◽  
Therapeutic Effects ◽  
Active Components ◽  
Plant Parts ◽  
Myrica Esculenta

Myrica esculenta (Myricaceae) is a popular medicinal plant most commonly found in the sub-tropical Himalayas. It is widely used in folk medicine to treat several ailments such as asthma, cough, chronic bronchitis, ulcers, inflammation, anemia, fever, diarrhea, and ear, nose, and throat disorders. Due to its multidimensional pharmacological and therapeutic effects, it is well recognized in the ayurvedic pharmacopeia. However, the recent upsurge in M. esculenta use and demand has led to illicit harvesting by the horticultural trade and habitat loss, pushing the plant to the brink of extinction. Thus, the present review aims to provide updated information on M. esculenta botany, ethnomedicinal uses, phytochemistry, pharmacological effects, toxicity, and conservation methods, as well as also highlight prospective for future research. Particular emphasis is also given to its antioxidant potential in health promotion. In-depth literature was probed by searching several sources via online databases, texts, websites, and thesis. About 57 compounds were isolated and identified from M. esculenta, and the available reports on physicochemical parameters, nutritional and high-performance thin-layer chromatography analysis of bioactive plant parts are portrayed in a comparative manner. Friendly holistic conservation approaches offered by plant biotechnology applications, such as micropropagation, germplasm preservation, synthetic seed production, and hairy root technologies are also discussed. Nonetheless, further studies are needed to propose the mechanistic role of crude extracts and other bioactives, and even to explore the structure–function relationship of active components.

scholarly journals Rapid Screening and Quantitative Determination of Active Components in Qing-Hua-Yu-Re-Formula Using UHPLC-Q-TOF/MS and HPLC-UV

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Xin Shao ◽  
Jie Zhao ◽  
Xu Wang ◽  
Yi Tao
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Chemical Constituents ◽  
Rapid Screening ◽  
Active Components ◽  
Limit Of Quantification ◽  
Unknown Compound ◽  
Salvianolic Acid ◽  
Tof Ms

Qing-Hua-Yu-Re-Formula (QHYRF), a new herbal preparation, has been extensively used for treating diabetic cardiomyopathy. However, the chemical constituents of QHYRF remain uninvestigated. In the present study, rapid ultrahigh-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) was used to qualitatively analyze the components of QHYRF. Qualitative detection was performed on a Kromasil C18 column through the gradient elution mode, using acetonitrile-water containing 0.1% formic acid. Twenty-seven compounds were identified or tentatively characterized, including 12 phenolic acids, nine monoterpene glycosides, two flavonoids, three iridoids, and one unknown compound. Among these, six compounds were confirmed by comparing with standards. A high-performance liquid chromatography (HPLC) method was developed to simultaneously determine the following six active components in QHYRF: danshensu, paeoniflorin, acteoside, lithospermic acid, salvianolic acid B, and salvianolic acid C. These HPLC chromatograms were monitored at 254, 280, and 320 nm. The method was well validated with respect to specificity, linearity, limit of detection, limit of quantification, precision, stability, and recovery. The HPLC-UV method was successfully applied to 10 batches of QHYRF.

scholarly journals A Validated High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Zofenopril Calcium and Hydrochlorothiazide in the Presence of the Hydrochlorothiazide Impurities: Chlorothiazide and Salamide

2018 ◽  
Vol 101 (4) ◽  
pp. 1031-1041 ◽  
Author(s):  
Mamdouh R Rezk ◽  
Ahmed S Fayed ◽  
Hoda M Marzouk ◽  
Samah S Abbas
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Degradation Products ◽  
Raw Materials ◽  
Drug Product ◽  
Hplc Method ◽  
Pharmaceutical Formulation ◽  
Significant Difference ◽  
Hptlc Method

Abstract The chromatographic analysis of either process-related impurities or degradation products is very important in the pharmaceutical industry. In this work, a simple, selective, and sensitive HPTLC method was developed and validated for the simultaneous determination of zofenopril calcium (ZOF) and hydrochlorothiazide (HCT) in the presence of the HCT impurities: A) chlorothiazide (CT) and B) salamide, in raw materials and in pharmaceutical formulation. The separation was carried out on HPTLC silica gel 60 F254 using ethyl acetate–glacial acetic acid–triethylamine (10 + 0.1 + 0.1, v/v/v) as a developing system. The separated bands were scanned densitometrically at 270 nm. Polynomial equations were used for the regression. Calibration curves were constructed for ZOF, HCT, CT, and salamide in the ranges of 0.5–10, 0.2–4, 0.05–1.4, and 0.05–1.0 μg/band, respectively. Different parameters affecting the suggested method, including developing systems of varying composition/ratios and different detection wavelengths, were studied to achieve the best resolution and precision with good sensitivity. System suitability parameters were also tested. The proposed method was validated as per the International Conference on Harmonization guidelines and was successfully applied for the quantification of the studied drugs in their pharmaceutical formulation, with no interference from excipients observed. The results obtained by the developed HPTLC method were compared statistically with those obtained by the reported HPLC method using Student’s t and F ratio tests, and no significant difference was obtained, indicating the ability of the proposed method to be used for routine analysis of drug product.

scholarly journals Evaluation of the influence of different doses of pre-sowing irradiation of seeds on the content of flavonoids in Hypericum perforatum L. medicinal raw material

2019 ◽  
Vol 25 ◽  
pp. 310-315 ◽  
Author(s):  
A. G. Salivon ◽  
K. V. Lystvan ◽  
S. V. Litvinov ◽  
S. A. Pchelovska ◽  
Yu. V. Shylina ◽  
...  
Keyword(s):  
High Performance ◽  
Hypericum Perforatum ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Raw Material ◽  
Flavonoid Content ◽  
X Ray ◽  
Hypericum Perforatum L ◽  
Ethanol Extracts ◽  
Different Doses

Aim. The purpose of this work was to determine the dose of g- and X-ray pre-sowing irradiation of seeds of Hypericum perforatum L., causing an increase in biomass and the accumulation of flavonoids in medicinal raw materials. Methods. X-ray and g-irradiation of seeds, biometric methods, flavonoids extraction, quantification of flavonoid content in extracts using spectrophotometry, qualitative and semi-quantitative analysis of extracts using high-performance liquid chromatography (HPLC), common statistical methods for processing and analysis of data. Results. It was shown that acute X-ray pre-sowing irradiation of H. perforatum seeds at a dose of 20 Gy leads to an increase in the crop of medicinal raw material without losing its pharmaceutical value. Irradiation also increases concentration of quercetin, routine, hyperoside in herbal medicines, grown from seeds, that was chronically irradiated with g-radiation in a total dose of 1 Gy. The HPLC analysis of extracts confirmed that the qualitative composition of ethanol extracts of H. perforatum did not change due to the effect of irradiation on the seeds. Conclusions. The obtained results confirm the possibility of application of pre-sowing irradiation of seeds of H. perforatum in the range of 1–35 Gy in order to increase its productivity and increase the pharmaceutical value of the medicinal raw material. Keywords: Hypericum perforatum L., ionizing irradiation, productivity, flavonoids.

scholarly journals Screening of the polyphenolic content of Amur grapes Rupr. and its identification by the method of tandem mass spectrometry

2021 ◽  
pp. 393-404
Author(s):  
М.П. Разгонова ◽  
А.Ш. Сабитов ◽  
Е.В. Перминова ◽  
Н.М. Михайлова ◽  
К.С. Голохваст
Keyword(s):  
High Performance ◽  
Ion Trap ◽  
Biologically Active ◽  
Vitis Amurensis ◽  
Active Components ◽  
Ionization Source ◽  
Grape Berries ◽  
Salvianolic Acids ◽  
Ethanol Extracts ◽  
First Time

Виноград амурский Vitis amurensis Ruprecht содержит большое количество полифенольных комплексов, являющихся биологически активными соединениями. В данной работе впервые проведено сравнительное метаболомное исследование биологически активных веществ дикого винограда, собранного из пяти различных мест Приморского и Хабаровского краёв. Для идентификации целевых аналитов в этанольных экстрактах ягод винограда использована высокоэффективная жидкостная хроматография (ВЭЖХ) в комплексе с ионной ловушкой amaZon SL, оснащенной источником ионизации электрораспылением ESI в режимах отрицательных и положительных ионов. Масс-спектрометр использовался в диапазоне сканирования m/z 100 - 1.700 для MС и МС/МС. Использовано фрагментирование 4-го порядка. Первичные масс-спектрометрические результаты показали присутствие 94 биологически активных соединений, соответствующих виду V. Amurensis ; причем сальвианоловые кислоты F, D и G, олеаноловая, урсоловая, миристолеиновая кислоты, берберицинин, меарнсетин, эскулин, невадензин, стигмастерол, фукостерол, флоризин, триптофан идентифицированы впервые в V. amurensis . Vitis amurensis Ruprecht contains a large number of polyphenolic compounds which are the biologically active components. In this work, for the first time, a comparative metabolomic study of biologically active substances of wild grapes collected from five different places of the Primorsky and Khabarovsk territories is carried out. To identify target analytes in ethanol extracts of grape berries, high performance liquid chromatography (HPLC) was used in combination with an amaZon SL ion trap (manufactured by BRUKER DALTONIKS, Germany) equipped with an ESI electrospray ionization source in negative and positive ion modes. The mass spectrometer was used in the scan range m / z 100 - 1.700 for MS and MS/MS. The fragmentation of the 4 order was used. Primary mass spectrometric results showed presence of 94 biologically active compounds corresponding to the species V. amurensis ; moreover, salvianolic acids F, D and G, oleanoic, ursolic, myristoleic acids, berbericinin, mearnsetin, esculin, nevadensin, stigmasterol, fucosterol, phlorizin, L-tryptophan were identified for the first time in V. amurensis .

scholarly journals Simultaneous HPTLC Determination of Rhein and Aloe-emodin in Senna Alata Leaves from Thailand and their Commercial Products

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200
Author(s):  
Savita Chewchinda ◽  
Pongtip Sithisarn
Keyword(s):  
High Performance ◽  
Raw Materials ◽  
Commercial Products ◽  
Average Percentage ◽  
System Validation ◽  
Relative Standard ◽  
Aloe Emodin ◽  
Senna Alata ◽  
Hptlc Method

A high performance thin layer chromatographic (HPTLC) method was developed and validated for simultaneous determination of rhein and aloe-emodin, major anthraquinone constituents, in S. alata leaves. The separation was performed on a silica gel 60 F254 HPTLC plate using ethyl acetate: methanol: water (100:17:10, v/v/v) as the development system. Validation of the analytical method for rhein and aloe-emodin promoted acceptable parameters. Good linearity in the range of 40–480 ng/band was obtained while intra-day and inter-day precisions were shown to be precise with relative standard deviations of less than 5%. The average percentage recoveries of rhein and aloe-emodin were 98.8% and 98.9%, suggesting acceptable accuracy. The content of rhein and aloe-emodin in S. alata leaves collected from 5 different provinces in Thailand analyzed by the validated HPTLC method were in the ranges of 0.098 ± 0.017 −0.30 ± 0.02%, w/w, and 0.081 ± 0.0006 - 0.34 ± 0.0009 %, w/w, respectively. Five commercial products of S. alata tea available in the market were purchased and analyzed for rhein and aloe-emodin contents. The contents of rhein and aloe-emodin in the tea samples were in the ranges of 0.085 ± 0.004 - 0.23 ± 0.04%, w/w, and 0.096 ± 0.006 - 0.30 ± 0.01 %, w/w, respectively. The HPTLC method is rapid, reliable, sensitive and economical for routine analysis of rhein and aloe-emodin contents in S. alata leaf raw materials and its commercial products.

A Validated Green HPTLC Method for Quantitative Determination of Dapoxetine Hydrochloride and Tadalafil in Bulk and Pharmaceutical Formulations

2020 ◽  
Vol 58 (4) ◽  
pp. 303-308 ◽  
Author(s):  
Ibrahim A Naguib ◽  
Maimana A Magdy ◽  
Basma H Anwar ◽  
Nessreen S Abdelhamid
Keyword(s):  
Quality Control ◽  
Mobile Phase ◽  
High Performance ◽  
Raw Materials ◽  
Pharmaceutical Formulations ◽  
Uv Detector ◽  
Ich Guidelines ◽  
Hptlc Method ◽  
Dapoxetine Hydrochloride

Abstract Dapoxetine hydrochloride (DAP) and Tadalafil (TAD) were separated and determined quantitatively using a validated green high-performance thin layer chromatographic (HPTLC) method in their binary mixtures either as raw materials or in pharmaceutical formulations. The concentration ranges were 0.1–1.6 and 0.2–2.5 μg/band for dapoxetine and tadalafil, respectively, with accuracies of 98.93% ± 0.62 and 99.26% ± 1.39, respectively. Silica gel HPTLC F254 plates were used to carry out the separation. The mobile phase used was a mixture of ethanol–ethyl acetate (1:9 by volume), which is environmentally green and harmless. Densitometric scanning with UV detector was used to detect the separated peaks at 222 nm. ICH guidelines were followed to validate the suggested method, and the results prove that they can be used for regular analysis in quality control laboratories with compatible results.

scholarly journals Simultaneous determination of polyphenol content Vitis amurensis Rupr. by tandem mass spectrometry

2021 ◽  
Vol 39 ◽  
pp. 02004
Author(s):  
Mayya P. Razgonova ◽  
Evgeniy I. Kislin ◽  
Andrey Sh. Sabitov ◽  
Elena V. Perminova ◽  
Natalia M. Mikhailova ◽  
...  
Keyword(s):  
High Performance ◽  
Ion Trap ◽  
Biologically Active ◽  
Vitis Amurensis ◽  
Active Components ◽  
Ionization Source ◽  
Grape Berries ◽  
Salvianolic Acids ◽  
Ethanol Extracts ◽  
First Time

Vitis amurensis Ruprecht contains a large number of polyphenolic compounds which are biologically active components. For the most efficient and safe extraction supercritical carbon dioxide was used. In this work, for the first time, a comparative metabolomic study of biologically active substances of wild grapes collected from five different places of the Primorsky and Khabarovsk territories is carried out. To identify target analytes in ethanol extracts of grape berries, high performance liquid chromatography (HPLC) was used in combination with an amaZon SL ion trap (manufactured by BRUKER DALTONIKS, Germany) equipped with an ESI electrospray ionization source in negative and positive ion modes. The mass spectrometer was used in the scan range m / z 100 - 1.700 for MS and MS / MS. Used fragmentation of the 4th order. Primary mass spectrometric results showed the presence of 94 biologically active compounds corresponding to the species V. amurensis, moreover, salvianolic acids F, D and G, oleanoic, ursolic, myristoleic acids, berbericinin, mearnsetin, esculin, nevadensin, stigmasterol, fucosterol, phlorizin, L-tryptophan identified for the first time in V. amurensis.

Development of Validated Stability Indicating HPTLC Method for Assay of Ozagrel and its Pharmaceutical Formulations

2018 ◽  
pp. 36-48 ◽  
Author(s):  
Vishal N Kushare ◽  
Sachin S Kushare
Keyword(s):  
High Performance ◽  
Linear Regression Analysis ◽  
Pharmaceutical Formulations ◽  
Base Hydrolysis ◽  
Assay Method ◽  
Related Substance ◽  
Stability Indicating ◽  
Regular Production ◽  
Hptlc Method

The present paper describes stability indicating high-performance thin-layer chromatography (HPTLC) assay method for Ozagrel in bulk drugs. The method employed TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of toluene: methanol: triethylamine (6.5: 4.0: 0.1 v/v/v). The system was found to give compact spot for Ozagrel (Rf value of 0.40 ± 0.010). Densitometric analysis of Ozagrel was carried out in the absorbance mode at 280 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.999 with respect to peak area in the concentration range 30 - 120 ng/spot. The developed HPTLC method was validated with respect to accuracy, precision, recovery and robustness. Also to determine related substance and assay determination of Ozagrel that can be used to evaluate the quality of regular production samples. The developed method can also be conveniently used for the assay determination of Ozagrel in pharmaceutical formulations. The limits of detection and quantitation were 4.069 and 12.332 ng/spot, respectively by height. Ozagrel was subjected to acid and alkali hydrolysis, oxidation, photochemical and thermal degradation. The drug undergoes degradation under acidic, basic, oxidation and heat conditions. This indicates that the drug is susceptible to acid, base hydrolysis, oxidation and heat. Statistical analysis proves that the method is repeatable, selective and accurate for the estimation of said drug. The proposed developed HPTLC method can be applied for identification and quantitative determination of Ozagrel in bulk drug and tablet formulation.

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