scholarly journals Phytochemical Screening and Antibacterial Activity of Grape Seed Extract

2021 ◽  
Vol 71 (2) ◽  
Author(s):  
Siva Jyothi J. ◽  
Bhavya Sri S. ◽  
Hasan Shaik T. ◽  
Samson Y. ◽  
Jawahar Babu P.
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Grape Seed Extracts ◽  
Seed Extracts

In the present investigation, preliminary phytochemical screening and antibacterial activity of diethyl ether, methanol and aqueous Grape seed extracts were examined. The phytochemical analysis revealed the presence of active ingredients such as flavonoids, phenols, terpenoids, saponins in Grape seed extracts. The antibacterial activity was performed by agar-well diffusion method using different solvent extracts. Three bacterial pathogens such as gram positive- Streptococcus pyogenes and gram negativePectobacterium carotovorum, Pseudomonas putida were used as test organisms. Among the three solvents used methanolic extract was found to be more active against tested pathogenic bacteria, as they showed potential phytochemical constituents. Among all the tested organisms Streptococcus pyogenes was found to be more sensitive by all the extracts. The results of present study supports the usage of Grape seeds and also suggests that methanolic grape seed extract possess compounds with antimicrobial property that can be used as antimicrobial agent for the therapy of infectious diseases caused by pathogens.

scholarly journals Cranberry and Grape Seed Extracts Inhibit the Proliferative Phenotype of Oral Squamous Cell Carcinomas

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Kourt Chatelain ◽  
Spencer Phippen ◽  
Jonathan McCabe ◽  
Christopher A. Teeters ◽  
Susan O'Malley ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Cancer ◽  
Cell Lines ◽  
Squamous Cell ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Oral Cancers ◽  
Grape Seed Extracts ◽  
Seed Extracts

Proanthocyanidins, compounds highly concentrated in dietary fruits, such as cranberries and grapes, demonstrate significant cancer prevention potential against many types of cancer. The objective of this study was to evaluate cranberry and grape seed extracts to quantitate and compare their anti-proliferative effects on the most common type of oral cancer, oral squamous cell carcinoma. Using two well-characterized oral squamous cell carcinoma cell lines, CAL27 and SCC25, assays were performed to evaluate the effects of cranberry and grape seed extract on phenotypic behaviors of these oral cancers. The proliferation of both oral cancer cell lines was significantly inhibited by the administration of cranberry and grape seed extracts, in a dose-dependent manner. In addition, key regulators of apoptosis, caspase-2 and caspase-8, were concomitantly up-regulated by these treatments. However, cranberry and grape seed extracts elicited differential effects on cell adhesion, cell morphology, and cell cycle regulatory pathways. This study represents one of the first comparative investigations of cranberry and grape seed extracts and their anti-proliferative effects on oral cancers. Previous findings using purified proanthocyanidin from grape seed extract demonstrated more prominent growth inhibition, as well as apoptosis-inducing, properties on CAL27 cells. These observations provide evidence that cranberry and grape seed extracts not only inhibit oral cancer proliferation but also that the mechanism of this inhibition may function by triggering key apoptotic regulators in these cell lines. This information will be of benefit to researchers interested in elucidating which dietary components are central to mechanisms involved in the mediation of oral carcinogenesis and progression.

Antibacterial activity of a grape seed extract and its fractions against Campylobacter spp.

Food Control ◽  
2013 ◽  
Vol 29 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Jose Manuel Silván ◽  
Elisa Mingo ◽  
Maria Hidalgo ◽  
Sonia de Pascual-Teresa ◽  
Alfonso V. Carrascosa ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Campylobacter Spp

scholarly journals A study on antibacterial effect of grape seed extracts in common clinical and drug resistant isolates

2016 ◽  
Vol 3 (3) ◽  
pp. 165
Author(s):  
Mohanakrishnan Kandasamy ◽  
Sowmya Nasimuddin ◽  
Jeevan Malayan ◽  
Nithyalakshmi J ◽  
Sumathi Gnanadesikan ◽  
...  
Keyword(s):  
Antibacterial Effect ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
Grape Seeds ◽  
Mrsa Strains ◽  
Agar Well Diffusion Assay ◽  
Grape Seed Extracts

<p class="abstract"><strong>Background:</strong> Grape seeds are proposed to have antimicrobial activity, antioxidant effect and various other benefits to mankind. A study was done to assess the antibacterial effect of grape seed extract against common clinical isolates and drug resistant pathogenic strains.</p><p class="abstract"><strong>Methods:</strong> Grape seed extract prepared was investigated for its antibacterial effect against 65 bacterial isolates obtained from clinical specimens by agar well diffusion assay and the results were compared with routinely used antibiotics namely, Gentamicin for the common clinical isolates, Vancomycin for MRSA strains and Amikacin for ESBL organisms respectively.</p><p class="abstract"><strong>Results:</strong> Grape seed extract produced moderate zone of inhibition ranging between 11-15 mm among the 35 test common clinical isolates namely <em>Staphylococcus aureus, Escherichia coli, Klebsiella </em>sp<em> </em>and<em> Pseudomonas aeruginosa</em>.  <em>E.coli </em>showed the highest susceptibility with zone ranging from 12-14 mm with increasing concentration of the extract starting from2 mg/ml to the highest being 20 mg/ml. Among the 30 drug resistant pathogenic strains like MRSA and ESBL producing organisms, the grape seed extract was found to be effective against 3 out of the 10 of MRSA and 2 out of the 10 of ESBL-<em>E.coli</em> at the highest concentration of 20 mg/ml. However, ESBL producing <em>Klebsiella </em>species were found to be resistant even to the highest concentration of the extract.  </p><p><strong>Conclusions:</strong> The results provide evidence that the grape seed extract could be a potential antibacterial agent and this effect can further be made evident with improved methodologies.</p>

scholarly journals Effect of Grape Seed Extract in Minimizing the Harmful Effects of Monosodium Glutamate on the Chicken Embryonic Retina to Show Scientific Miracles In the Holy Quran

2020 ◽  
pp. 7-18
Author(s):  
Fawzya Al- Gamdi ◽  
Nouf Al- Harbi
Keyword(s):  
Negative Impact ◽  
Monosodium Glutamate ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
The Body ◽  
Control Group ◽  
Beverage Industry ◽  
Potential Improvement ◽  
Grape Seed Extracts

In 1969, a Canadian laboratory announced that its studies on synthetic alkaline (cyclamate) proved to be a carcinogen despite its use for more than twenty years in the beverage industry. There are many additives that have raised the controversy about the large extent of safety to consumers and the most famous controversy and consumption is the article of monosodium glutamate (MSG) which touched research on the negative impact on samples of research experiments. This research aims to study the effect (MSG) on the formation of retina in chicken embryos and the potential improvement effects of GSE grape seed extracts during incubation as part of the solutions provided in the event of the long term use of MSG and its accumulation in the body Human. The experimental groups were divided into 4 groups based on the injection period and the injected substances. The first control group(Injected with saline), the second group, treated with  MSG, the 3rd group were treated with Monosoudim glutamate and grape seed extract at days 0,1 of incubation, whereas the 4thgroup,was treated with MSG and then the GSE (M – G) were given at3,4 days post MSG . The effect of the experimental materials on the development of the retina at the age of 14 was investigated in the current work. The results showed that the treatment of embryos with MSG dose (0.1 ml) caused many of the abnormalities at the level of tissue formation of the retina, and after the treatment of the fetus with GSE was noted to reduce the damage to the tissue of the retina. The results showed that the treatment of embryos with MSG dose (0.1 ml) caused many of the abnormalities at the level of tissue form of the retina, and after the treatment of the fetus with GSE was noted to reduce the damage to the tissue of the retina.

scholarly journals Phytochemical Analysis, In vitro Antibacterial Activity and Rate of Kill of Different Fractions of Dacryodes edulis Leaf

2020 ◽  
pp. 23-35
Author(s):  
R. E. Hassan-Olajokun ◽  
A. M. Deji-Agboola ◽  
O. O. Olasunkanmi ◽  
T. A. Banjo ◽  
O. Olaniran ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Zones Of Inhibition ◽  
Time Kill ◽  
Dacryodes Edulis

Dacryodes edulis is a fruit tree attaining a height of 18–40 meters mostly grown around the house and rarely in the forest. It has a relatively short trunk and a deep, dense crown and it is native to Africa hence often called African pear. The leaves are a compound with 5-8 pairs of leaflets. Acetone extract/solvent fractions of the leaves of Dacryodes edulis were used. The in vitro antibacterial activities and rate of kill of different fractions were investigated. The phytochemical screening was done by some chemical tests, antibacterial activity by agar well diffusion method and rate of kill was carried out on S. aureus and E. coli organisms. The fractions exhibited antibacterial activities with zones of inhibition ranged between 20 and 30 mm by Aqueous (AQU) fraction while Dichloromethane (DCM) fraction ranged between 22 and 32 mm. The standard antibiotics, streptomycin ranged between 10 and 20 mm and ampicillin between 11 and 27 mm. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of both fractions had range values between 0.78 and 6.25 mg/mL. The phytochemical screening revealed the presence of alkaloids, tannins, saponins, flavonoids, terpenoid and glycoside. Overall, the two fractions had better activities than the standard antibiotics used. The time kill assay showed that the percentage of the cells killed increased with increasing concentrations of the fractions, as well as, contact time intervals. The AQU fraction killed 100% of Escherichia coli and Staphylococcus aureus cells at concentration of 4.68 mg/mL (3 X MIC) within 120 min while DCM fraction killed 100% of Escherichia coli cells within 90 min at a concentration of 4.68 mg/mL and killed 100% of S. aureus cells within 120 min at a concentration of 3.12 mg/mL (2X MIC). In conclusion, D. edulis leaf fraction has a broad spectrum antibacterial activity, with the AQU and DCM fractions being bactericidal as exemplified by the killing rate and MIC index of 2 (ratio of MBC/MIC) for both fractions.

scholarly journals Assessment of antimicrobial activity of sodium hypochlorite, calcium hypochlorite and grape seed extract against Enterococcus faecalis

2018 ◽  
Vol 32 (3) ◽  
pp. 136
Author(s):  
Larissa Taís Soligo ◽  
Doglas Cecchin ◽  
Matheus Albino Souza ◽  
Ezequiel Santin Gabrielli ◽  
Huriel Scartazzini Palhano ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Saline Solution ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
The Other ◽  
Diffusion Method ◽  
Calcium Hypochlorite

OBJECTIVE: This study aimed to assess the antimicrobial action of calcium hypochlorite [Ca(OCl)2] at concentrations of 2.5% and 6%, and of grape seed extract (GSE) at concentrations of 10%, 30%, and 50%, against Enterococcus faecalis, comparing it to the action of 6% sodium hypochlorite (NaOCl).METHODS: Saline solution was used as negative control. The inhibition halos of microbial growth were verified by the agar disk diffusion method. Twelve Petri plates were used for seeding with culture medium of approximately 5 mm in thickness. In each plate, 5 disks of pure and sterile antibiogram, soaked in the substances to be tested, were used and taken to the plate containing the seededbacterial strain. The plates remained in aerobic bacteriological incubator for 24 h at 37°C temperature. After 24 h, the inhibition halos were measured with a digital caliper. Analysis of Variance (ANOVA) was used for statistical analysis followed by Tukey's complementary test, at 5% significance.RESULTS: The 6% Ca(ClO)2 presented inhibition halo statistically higher than the other solutions (p<0.05), followed by 2.5% Ca(ClO)2, which was statistically similar to 6% NaOCl (p>0.05). The GSE concentrations resulted in lower inhibition halos of active substances and the different concentrations were similar to each other. Lastly, saline solution presented the same inhibition halos in all groups (p<0.05).CONCLUSION: It may be concluded that 6% Ca(OCl)2 presented higher antimicrobial activity than 6% NaOCl. On the other hand, all GSE concentrations were lower than NaOCl and Ca(OCl)2.

scholarly journals Uji Efektivitas Ekstrak Biji Kakao (Theobroma Cacao L.) Sebagai Antibakteri Dalam Berbagai Konsentrasi pada Streptococcus Pyogenes

2021 ◽  
Vol 36 (1) ◽  
pp. 8-13
Author(s):  
Dame J Pohan ◽  
Angela P Kakerissa ◽  
Evy S Arodes
Keyword(s):  
Streptococcus Pyogenes ◽  
Antibacterial Effect ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Seed Extract ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
Long Time ◽  
Theobroma Cacao L ◽  
Seed Extracts

ABSTRAK Biji kakao adalah buah yang dikenal sejak dahulu sebagai buah yang memiliki peran penting dalam bidang kesehatan, salah satunya sebagai antibakteri. Biji kakao mengandung senyawa flavonoid, tanin, dan alkaloid yang memiliki efek antimikroba. Tujuan penelitian ini untuk mengetahui efektivitas ekstrak biji kakao terhadap bakteri Streptococcus pyogenes, yaitu dengan cara mengukur zona hambat menggunakan metode difusi Kirby-Beuer dengan konsentrasi ekstrak biji kakao 20%, 40%, 60%, 80% dan 100%. Hasil penelitian menunjukan ekstrak biji kakao dapat efektif memberikan efek antibakteri mulai dari konsentrasi terkecil 20% dengan rata-rata diameter zona hambat 8.07 mm sampai konsentrasi terbesar 100% dengan rata-rata diameter zona hambat 10.98 mm. Sedangkan rata-rata diameter zona hambat antibiotik Ampisilin sebagai kontrol (+) lebih besar dari konsentrasi ekstrak biji kakao. Berdasarkan data tersebut dapat disimpulkan ekstrak biji kakao dapat efektif menghambat pertumbuhan bakteri Streptococcus pyogenes  Kata kunci: Kirby-Bauer, Zona Hambat, Antimikroba ABSTRACT Cocoa Seed is a fruit that has been known for a long time as a fruit that has an important role in health, which is as an antibacterial. Cocoa seeds contain a high composition of flavonoids, tannins, and alkaloids which have antimicrobial effects. The aim of this research was to study the effect of cocoa seed extracts against Streptococcus pyogenes, by measuring the inhibition zone using the Kirby-Bauer diffusion method using concentrations of cocoa seed extract 20%, 40%, 60%, 80% and 100%. The results showed that chocolate seed extract could effectively provide an antibacterial effect ranging from a concentration of 20% with an average diameter of inhibition zone of 8.07 mm to the largest concentration of 100% with an average zone diameter of inhibition of 10.98 mm. While the average diameter of the inhibitory zone of antibiotic ampicillin as a control (+) is greater than the concentration of cocoa seed extract. Based on these data, it can be concluded that cocoa seed extract can effectively inhibit the growth of Streptococcus pyogenes. Keywords: Kirby-Bauer, Inhibitory zone, Antimicrobial

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