COMPARATIVE EFFECT OF THREE DIFFERENT ENERGY SOURCES ON HEMATOLOGY AND SERUM BIOCHEMISTRY OF RABBITS

2020 ◽  
Vol 2 (1) ◽  
pp. 17
Author(s):  
Sani Babangida Salihu
Keyword(s):  
Adverse Effect ◽  
Alkaline Phosphatase ◽  
Blood Cells ◽  
Serum Biochemistry ◽  
Energy Sources ◽  
Sample Collection ◽  
Normal Range ◽  
Comparative Effect ◽  
Group A ◽  
Inclusion Level

Purpose: This study examined the effect of three different energy sources on hematology and serum biochemistry of rabbits. Methodology:  Eighteen seven-weeks old rabbits where randomly divided into three groups (A, B and C), each consisting of six rabbits. Group A, B and C were given maize, sorghum and millet respectively as their sources of energy and were fed for a period of five weeks before sample collection and analysis. Results: The results showed, that there was no significant different (P>0.05) in the hematology and serum biochemistry. Nevertheless, the red blood cells in all the treatment fell below the normal range. It was observed that the alkaline phosphatase of all the experimental rabbits were above the normal range. This study suggests that our test materials were not toxic to the muscle, kidney and liver of rabbits at 52.21% inclusion level. Unique Contributions to Theory Practice and Policy: This implies that sorghum or millet can completely replace maize in rabbit diet with no adverse effect on the hematology and serum biochemistry parameters of rabbits.

Naphthaloyl group: a new selective amino protecting group for deoxynucleosides in oligonucleotide synthesis

1988 ◽  
Vol 66 (12) ◽  
pp. 2989-2994 ◽  
Author(s):  
Archana Dikshit ◽  
Manjula Chaddha ◽  
R. K. Singh ◽  
Krishna Misra
Keyword(s):  
Adverse Effect ◽  
Alkaline Phosphatase ◽  
Solid Phase ◽  
Protecting Group ◽  
Oligonucleotide Synthesis ◽  
Snake Venom Phosphodiesterase ◽  
Elemental Analyses ◽  
Group A ◽  
Cyclic Imides ◽  
As Solid Phase

The naphthaloyl group has been found to be a selective amino protecting group for deoxycytidine, deoxyadenosine, and deoxyguanosine in oligodeoxyribonucleotide synthesis. All three protected monomers obtained (78–85%), being six-membered cyclic imides, were fairly stable. These protected monomers were used successfully for the preparation of dimers (phosphodiester approach) and tetramers (phosphotriester approach) in solution as well as solid phase, respectively. The group acted as a purification tool due to its high lipophilicity. No adverse effect has been observed either on the glycosidic bond (depurination) or the internucleotidic bond during its removal. The monomeric units were characterized by UV, NMR, and elemental analyses whereas the tetramers were characterized by enzymatic hydrolyses with snake venom phosphodiesterase followed by alkaline phosphatase.

scholarly journals An Evaluation of Morphological Changes and Deformability of Suspended Red Blood Cells Prepared Using Whole Blood with Different Hemoglobin Levels of Tibetans

2021 ◽  
pp. 1-10
Author(s):  
Rui Zhong ◽  
Dingding Han ◽  
Xiaodong Wu ◽  
Hong Wang ◽  
Wanjing Li ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Morphological Changes ◽  
Osmotic Fragility ◽  
Spherical Shape ◽  
Hypoxic Environment ◽  
Wide Range ◽  
Group A ◽  
Cell Membrane Protein

Background: The hypoxic environment stimulates the human body to increase the levels of hemoglobin (HGB) and hematocrit and the number of red blood cells. Such enhancements have individual differences, leading to a wide range of HGB in Tibetans’ whole blood (WB). Study Design: WB of male Tibetans was divided into 3 groups according to different HGB (i.e., A: >120 but ≤185 g/L, B: >185 but ≤210 g/L, and C: >210 g/L). Suspended red blood cells (SRBC) processed by collected WB and stored in standard conditions were examined aseptically on days 1, 14, 21, and 35 after storage. The routine biochemical indexes, deformability, cell morphology, and membrane proteins were tested. Results: Mean corpuscular volume, adenosine triphosphate, pH, and deformability were not different in group A vs. those in storage (p > 0.05). The increased rate of irreversible morphology of red blood cells was different among the 3 groups, but there was no difference in the percentage of red blood cells with an irreversible morphology after 35 days of storage. Group C performed better in terms of osmotic fragility and showed a lower rigid index than group A. Furthermore, SDS-PAGE revealed similar cross-linking degrees of cell membrane protein but the band 3 protein of group C seemed to experience weaker clustering than that of group A as detected by Western Blot analysis after 35 days of storage. Conclusions: There was no difference in deformability or morphological changes in the 3 groups over the 35 days of storage. High HGB levels of plateau SRBC did not accelerate the RBC change from a biconcave disc into a spherical shape and it did not cause a reduction in deformability during 35 days of preservation in bank conditions.

Iron and Hematological Status among Adolescent Athletes Tracked through Puberty

1995 ◽  
Vol 7 (3) ◽  
pp. 253-262
Author(s):  
Noreen D. Willows ◽  
Susan K. Grimston ◽  
David J. Smith ◽  
David A. Hanley
Keyword(s):  
Adverse Effect ◽  
Iron Deficiency ◽  
Serum Ferritin ◽  
Direct Evidence ◽  
Iron Status ◽  
Normal Range ◽  
Pubertal Stage ◽  
Physically Active ◽  
Pubertal Stages ◽  
Prepubertal Boys

This study assessed change in hematological status among physically active children as they progressed through puberty. Values for serum ferritin, hemoglobin, and hematocrit at all stages of puberty were within the normal range of reference values. Significant changes in serum ferritin were not detected in the different pubertal stages, although serum ferritin was highest in prepubertal boys and girls. There were no significant differences in marginal or deficient iron stores between the sexes at any pubertal stage, suggesting that gender was not predisposing for iron deficiency; however, girls had a greater overall incidence for both measures. With more children under consideration, these trends may have reached significance. Boys in TS4 and TS5 had higher hemoglobin and hematocrit compared with earlier stages of puberty, and compared with girls at the same stages of puberty. This can be explained by testosterone production in boys. Among girls, pubertal progression had no significant effect on hemoglobin or hematocrit. In the absence of controls, there was no direct evidence that involvement in sports had an adverse effect on iron status.

scholarly journals OCCURRENCE OF 19S AND 7S ANTI-IGGS DURING HYPERIMMUNIZATION OF RABBITS WITH STREPTOCOCCI

1972 ◽  
Vol 136 (4) ◽  
pp. 799-815 ◽  
Author(s):  
Viktor A. Bokisch ◽  
David Bernstein ◽  
Richard M. Krause
Keyword(s):  
Red Blood Cells ◽  
Blood Group ◽  
Pneumococcal Vaccine ◽  
Blood Cells ◽  
Genetic Influence ◽  
Group A

All 110 rabbits immunized with Group A, A-variant, and C streptococcal vaccines produced 19S anti-IgG in addition to antibodies to the streptococcal carbohydrates. 19S anti-IgG was detected by hemagglutination of rabbit red blood cells coated with rabbit anti-blood group F antibody. Antisera of 88 of these animals were also tested for 7S anti-IgG with a coprecipitation assay. This assay is based on the coprecipitation of 7S anti-IgG with complexes of streptococcal carbohydrate and anti-carbohydrate antibody. 50 of the 88 anti-Group C streptococcal antisera contained 7S anti-IgGs. In eight antisera the concentration was greater than 5 mg/ml. The data suggest a genetic influence on the occurrence of 7S anti-IgG. The eight rabbits which produced more than 5 mg/ml of 7S anti-IgG belonged to three related families. Moreover, there were families in which almost every member produced 7S anti-IgG and other families in which only 30% of the members manufactured 7S anti-IgG. The streptococcal vaccine was an especially efficient stimulus for the production of 19S anti-IgG, whereas the pneumococcal vaccine was much less effective in this respect. Furthermore, 7S anti-IgGs were not detected in antipneumococcal antisera, although the concentration of anti-capsular antibodies was similar to that of anti-carbohydrate antibodies in antistreptococcal antisera.

A CLINICO-COMPARATIVE STUDY ON THE EFFECT OF LASHUNA SIDDHA TAILPANA POORVAK VAMANA KARMA AND NITYA VIRECHANA BY GOMUTRA HARITAKI IN THE MANAGEMENT OF HYPOTHYROIDISM

2021 ◽  
Vol 12 (4) ◽  
pp. 49-55
Author(s):  
Sheetal R Tokle
Keyword(s):  
Comparative Study ◽  
Thyroid Stimulating Hormone ◽  
Normal Range ◽  
End Of Treatment ◽  
Group A ◽  
Insignificant Difference ◽  
Randomized Control ◽  
Drug Dependent ◽  
Group B ◽  
Stimulating Hormone

Hypothyroidism is a condition in which thyroid gland doesn’t produce enough thyroid hormone. This is more prevalent among women. Management through levothyroxine is safe & may bring the value of Thyroid stimulating hormone and thyroxine to normal range but the increased dosage and continuous medication are cost expensive and make the patient into drug dependent till the end of mortal life. So, better, therapy is needed for the society through the heritage of Ayurveda especially with Shodhana therapy. Aim of Clinico-comparative study was to evaluate and compare the efficacy of Lashuna siddha Tailpana Poorvak Vamana Karma and Nityavirechana by Gomutra Haritaki in the management of Hypothyroidism. Study was conducted at Govt. Akhandanada Ayurvedic hospital, Ahmedabad, Gujarat. This study was Open labelled parallel randomized control trial. 15 patients were treated with Lashuna siddha Tailpana Poorvak Vamana Karma in group A. 15 patients was treated with Nitya Virechana by Gomutra Haritaki in group B. Washout period was 14 days. Triiodothyronine and thyroxine were compared at the end of treatment by paired t-test and Mann Whitney-U test. Lashuna siddha Tailpana Poorvak Vamana Karma was more beneficial than Nitya Virechana by Gomutra Haritaki. Insignificant difference was found on subjective and objective parameters (Weight gain, Basal metabolic rate, Serum triiodothyronine) but significance difference found on objective parameters (Thyroid stimulating hormone, Serum thyroxine).

scholarly journals STUDIES ON HEMAGGLUTINATION AND HEMOLYSIS BY ESCHERICHIA COLI ANTISERA

1952 ◽  
Vol 96 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Erwin Neter ◽  
Lee F. Bertram ◽  
Dorothy A. Zak ◽  
Miriam R. Murdock ◽  
Carl E. Arbesman
Keyword(s):  
Escherichia Coli ◽  
Guinea Pig ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Homologous Blood ◽  
E Coli ◽  
Blood Group A ◽  
Human Red Blood Cells ◽  
Order Of Magnitude ◽  
Group A

A study on hemagglutination and hemolysis by Escherichia coli O111 and O55 (rabbit) antisera and on hemagglutination and hemolysis inhibition by E. coli O111 and O55 antigens revealed the following facts. 1. Red blood cells of man, dog, rabbit, guinea pig, sheep, rat, and chicken adsorb E. coli O111 and O55 antigens and thus become specifically agglutinable by the homologous E. coli antisera. 2. The adsorption of these E. coli antigens is a function of the concentration of the antigen, the time (from 5 minutes to 2 hours) of treatment of the red blood cells with the antigen, and the concentration of the red blood cells used. 3. Red blood cells of man and sheep adsorb simultaneously both antigens, as indicated by the fact that both antisera give agglutination of all red blood cells. Complete agglutination does not occur when a mixture of red blood cells treated separately with the two antigens is added to one or the other of the two antisera. 4. Treatment of red blood cells of man with one of the antigens does not block the adsorption of the second antigen. Human cells treated with either or both antigens are still agglutinated by the homologous blood group (A, B, and Rh)-specific antibodies. 5. In the presence of guinea pig complement, E. coli O111 and O55 antisera produce hemolysis of modified human red blood cells in titers of the same order of magnitude as those giving hemagglutination and bacterial agglutination. The same antisera produce hemolysis of sheep cells treated with the identical antigens in titers exceeding by far those giving agglutination of modified human or sheep red blood cells. 6. Both sediment and supernate of a boiled E. coli suspension are capable of modifying red blood cells for E. coli hemagglutination; in contrast, the supernate obtained from an unboiled suspension and then heated does not modify red blood cells for hemagglutination, although it contains the antigen which can specifically adsorb E. coli antibodies, as shown by means of the hemagglutination and hemolysis inhibition tests. 7. Both the unheated and the boiled suspensions of E. coli O111 and O55 inhibit hemagglutination and hemolysis specifically. 8. Rabbit red blood cells modified by either E. coli O111 or 055 antigens, upon intravenous injection into rabbits, engender specific E. coli antibodies. The significance of the results is discussed.

scholarly journals AB0-incompatibility of mother and fetus: the role of anti-glycan alloantibodies in the hemolytic disease of newborns

2021 ◽  
Vol 23 (1) ◽  
pp. 17-34
Author(s):  
P. S. Obukhova ◽  
A. V. Kachanov ◽  
N. A. Pozdnyakova ◽  
M. M. Ziganshina
Keyword(s):  
Red Blood Cells ◽  
Blood Group ◽  
Blood Cells ◽  
Hemolytic Disease ◽  
Disease Condition ◽  
Mother And Child ◽  
Group A ◽  
Group B ◽  
Maternal Igg

The mother and fetus incompatibility due to Rh-factor, blood group or other blood factors can lead to hemolytic disease of the fetus and newborn (HDN). HDN is a clinical disease condition of the fetus and newborn as a result of hemolysis, when maternal IgG alloantibodies cross the placenta and destroy the red blood cells of the fetus and newborn. The child disease begins in utero and can dramatically increase immediately after birth. As a result, hyperbilirubinemia and anemia develop, that can lead to abortions, serious complications, or death of the neonates in the absence of proper therapy. The range of HDN has changed significantly now compared to previous decades. Half a century ago, HDN was considered an almost complete synonym of RhD-alloimmunization, and this was a frequent problem for newborns. By now due to the high effective of Rh-conflict prevention, immunological AB0-conflicts have become the most common cause of HDN. The review aimes to one of the main causes of jaundice and anemia in neonates at present, i.e. HDN due to immunological AB0-conflict of mother and newborn (AB0-HDN). The main participants of the AВ0- incompatibility mother and child are considered, namely A- and B-glycans, as well as the corresponding anti-glycan alloantibodies. Close attention is paid to the structure features of glycan alloantigens on the red blood cells of the fetus and adult. The possible correlation of the frequency and severity of HDN with the blood group of mother and child, as well as with the titer of maternal alloantibodies, has been considered. The influence of immunoglobulin G subclasses on the AB0-HDN development has been evaluated. In most cases, AB0-HDN appear when the mother has the blood group 0, and the fetus has the group A (subgroup A1) or the group B. Other rare incidences of AB0-incompatibility with severe course are occurred. As a whole the etiology of AB0-HDN is complex and the HDN severity is influenced by many factors. The authors have analyzed statistical data, as well as the prevalence of AB0-incompatibility and AB0-HDN in various regions of the world. Current approaches to the diagnosis of AB0-HDN are discussed in addition. By now the problems of AB0- HDN occurrence and developing of ways to overcome this disease remain relevant.

Typing of human group A rotavirus with alkaline phosphatase-labeled oligonucleotide probes

1992 ◽  
Vol 37 (3) ◽  
pp. 192-196 ◽  
Author(s):  
Orntipa Sethabutr ◽  
Suvath Hanchalay ◽  
Udom Lexomboon ◽  
Ruth F. Bishop ◽  
Ian H. Holmes ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Oligonucleotide Probes ◽  
Human Group ◽  
Group A Rotavirus ◽  
Group A

Assessing the haematology and serum chemistry of Uda rams fed different Kanwa based mineral licks

2021 ◽  
Vol 6 (6) ◽  
pp. 209-215
Author(s):  
A. Abdulkarim ◽  
◽  
K. M. Aljameel ◽  
Keyword(s):  
Reference Range ◽  
Health Hazard ◽  
Serum Biochemistry ◽  
Serum Chemistry ◽  
Normal Range ◽  
Blood Profile ◽  
Normal Reference Range ◽  
Normal Reference ◽  
Mineral Licks ◽  
Sheep Production

This study was conducted at the Department of Animal Science’s Teaching and Research farm of Usmanu Danfodiyo University, Sokoto to evaluate the effect of feeding different kanwa based mineral licks on blood profile of Uda rams. Blood of twenty (20) yearlings Uda rams aged by dentition were used to evaluate the effect of Kanwa¬-based mineral blocks. Four (4) animals were allotted to each treatment (Kanwa block) with each animal serving as a replicate. The Kanwa used were Kanwan Bai-Bai, Kanwan Kolo, Hogga, Balma and conventional mineral lick to represent treatment 1, 2, 3, 4 and 5 respectively. Data was collected at the end of the experiment. The result of haematological analysis showed significant (p<0.05) differences in all the parameters analysed except PCV, MCH, WBC and eosinophil; the result indicated that all the values where within the normal reference range except in MCHC where animals placed in T2 and T3 shows values slightly above the normal range. The result of serum biochemistry showed that all the values measured were within the normal reference range for sheep except the albumin of animals placed in T3 which had values slightly above the normal range. The serum mineral composition showed that Potassium levels were higher in T2 and lower in T4; however, there was no difference between animals placed in treatments 1, 2 and 3, so also between treatments 1, 3 and 4. The study concluded that Kanwa positively affected sheep production with no adverse effect on both haematological and serum chemistry, hence little health hazard is associated with feeding Kanwa-based blocks on growing Uda rams.

Effect Of Citrullus Lanatus Juice On Hemoglobin, Red Blood Cells And Liver Enzyme; An Experimental Study

2019 ◽  
Vol 09 (04) ◽  
pp. 257-260
Author(s):  
Shahid Ali ◽  
Nuzhat Sultana ◽  
Muslim Abbas ◽  
Zareen Naz ◽  
Muhammad Akbar Hassan ◽  
...  
Keyword(s):  
Experimental Study ◽  
Red Blood Cells ◽  
Liver Enzyme ◽  
Blood Cells ◽  
Citrullus Lanatus ◽  
Treated Group ◽  
Control Group ◽  
Group A ◽  
Group B ◽  
Post Hoc

Objective: To determine effect of citrullus lanatus juice on hemoglobin and Serum Glutamic Pyruvic Transaminase (SGPT) level. Study design and setting: It was an experimental study conducted on healthy rabbits for sixty days in the research department of pharmacy University of Karachi. Methodology: Current study was planned to evaluate effect of Citrullus lanatus juice on red blood cells, hemoglobin and liver enzyme. 60 days study was performed at 2 different doses i.e 3and 6ml/kg on rabbits. These rabbits were from either gender and were divided into 3 groups their weight ranges from 1200 to 1800 grams. Group A is control group (Normal saline 6ml/kg), Group B is treated group (3 ml/kg), Group C is treated group (6ml/kg). After taking mean of all values they are compared with control group. Significance of mean can be estimated by Tukes Post Hoc Test. P<0.05 estimated as significant. Results: It was found that count of red blood cells rises significantly along with rise in hemoglobin level. As far as liver enzyme serum glutamate pyruvate transaminase (SGPT) was concerned its concentration decreases slightly. Conclusion: Citrullus lanatus juice contain ingredients which are important for RBC hemoglobin and synthesis .It also contains important antioxidants that have organoprotective role due to which SGPT level decreases even in healthy animals as compared to control groups

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