scholarly journals NEFROPROTECTOR EFFECT OF CORN SILK (Stigma maydis) ETHANOL EXTRACT ON GENTAMICIN INDUCED IN WISTAR RATS

2021 ◽  
Vol 6 (9) ◽  
pp. 17-26
Author(s):  
Nessa Nessa ◽  
Ridho Andriza ◽  
Hazli Nurdin ◽  
Ridho Asra
Keyword(s):  
Serum Creatinine ◽  
Cell Damage ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Histopathological Analysis ◽  
Corn Silk ◽  
White Rats

Corn silk (Stigma maydis) is one part of the corn plant that contains useful chemical compounds, including flavonoid compounds. This study aimed to determine the nephroprotective effect of corn silk ethanol extract on male white rats. This type of research is an experimental study, where animals were grouped into 5 groups consisting of the negative control group only given 0.5% NaCMC suspension orally, the positive group given gentamicin 80 mg/kgBW intraperitoneally. the treatment group of corn silk extract doses of 250, 500, and 1000 mg/KgBW, the group was given an extract suspension orally for 8 days. On the 3rd day of extract administration, gentamicin was administered intraperitoneally 1 hour after administration of the extract until the 8th day extract administration. On the 8th day urine was collected for microscopic examination and on the 9th day blood was taken from the orbital vein of the eye to measure serum creatinine and urea levels, after that the animals were sacrificed and their ratio of kidney weight and histopathology was calculated. gentamicin showed a significant decrease in serum creatinine and urea levels when compared to the positive control group. Histopathological analysis also showed an increase in cell regeneration and the lowest percentage of tubular epithelial cell damage among other doses. Based on the results of the study, it was concluded that there was a nephroprotector effect on the ethanolic extract of corn silk and a variation of the dose of 1000 mg/KgBW was effective as a nephroprotector.

scholarly journals Analgesic Activity of Ethanol Extract of Temu Giring Rhizome (Curcuma heyneana Val & Zijp) in Mice

2018 ◽  
Vol 1 (2) ◽  
pp. 9-13
Author(s):  
Marianne ◽  
Khairunnisa ◽  
Wilda
Keyword(s):  
Analgesic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Significance Level ◽  
Treatment Groups ◽  
Infra Red ◽  
Thermal Induction

Temu giring (Curcuma heyneana Val & Zijp) is a traditional medicinal plant that is believed in community as an analgesic. The objective of this research was to determine the analgesic activity of the C. heyneana rhizome by using infra red (IR) thermal induction method in mice. Mice were divided into 7 groups. Group 1 served as negative control, group 2,3,4,5 served as treatment groups which is  given ethanolic extract of C. heyneana rhizome at  dose of 5, 25, 125, and 625 mg/kg respectively, group 6 and 7 served as  comparable groups, given antalgin 65 mg/kg and morphine sulphate 1.3 mg/kg respectively. The observation have been done, included to pain resistance of mice which exposed by infra red (IR) every 10 minutes for 80 minutes. The data were analyzed by ANOVA at the significance level of 95%. Ethanolic extract of C. heyneana at the doses of 25, 125, and 625 mg/kg had significant effect to reduce the pain compared to the negative control (p<0.05). Ethanolic extract of C. heyneana rhizome at dose of 125 mg/kg, had the same effect to antalgin 65 mg/kg  (p≥0.05), while the ethanolic extract of C. heyneana at the dose of 625 mg/kg had the same effect as morphine sulfate 1.3 mg/kg (p≥0.05). It can be concluded that ethanolic extract of C. heyneana rhizome has analgesic activity.   Keywords: temu giring, analgesic, Curcuma heyneana, rhizome

scholarly journals Uji Hepatoprotektor Ekstrak Etanol Buah Pepino (Solanum muricatum) Terhadap Tikus Putih Jantan Galur Wistar Yang Diinduksi CCL4

2021 ◽  
Vol 16 (1) ◽  
pp. 167
Author(s):  
Muhammad Asri ◽  
Ummu Kalsum
Keyword(s):  
Experimental Design ◽  
Vitamin C ◽  
Design Research ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
White Rats ◽  
Hepatoprotector Activity ◽  
Solanum Muricatum

ABSTRACTBuah pepino memiliki kandungan seyawa yaitu alkaloid, flavonoid, vitamin C yang dapat berkhasiat sebagai hepatoprotektor. Penelitian ini bertujuan untuk mengetahui aktivitas hepatoprotektor ekstrak etanol buah pepino (Solanum muricatum) terhadap tikus putih jantan  yang diinduksikan ccl4 dengan dosis ekstrak dibagi menjadi tiga kelompok yaitu 100 mg/kgBB, 200 mg/kgBB dan 400 mg/kgBB. Penelitian ini merupakan jenis eksperimental menggunakan rancangan  penelitian True Experimental design. Pengujian aktivitas hepatoprotektor dengan parameter acuan nya adalah kadar SGOT dan SGPT.  Analisis hasil pengukuran SGOT dan SGPT dihitung dengan menggunakan ANOVA satu arah.  Hasil pengukuran  kadar SGPT dan SGOT dengan dosis 100 mg/kgBB, 200 mg/kgBB dan 400 mg/kg BB terjadi penurunan yang berbeda-beda .Pada SGOT penurunan tertinggi kadar terdapat pada ekstrak etanol buah pepino 100 mg/kgBB diikuti ekstrak etanol buah pepino 200 mg/kgBB lalu kelompok ekstrak etanol buah pepino 400 mg/kgBB kemudian kelompok positif dan terakhir kelompok negative .Dan pada SGPT penurunan kadar SGPT tertinggi pada kelompok control positif diikuti kelomok ekstrak etanol  buah pepino dengan konsentrasi 100 mg/kgBB, lalu ekstrak etanol buah pepino dengan konsentrasi 200 mg/kgBB, kemudian konsentrasi 400 mg/kgBB dan terakhir kelompok control negative. Berdasarkan penelitian yang telah dilakukan, dapat disimpulkan bahwa buah pepino (Solanum muricatum) memiliki aktivitas hepatoprotektor pada konsentrasi 100 mg/kgBB pada kadar SGPT dan SGOT . Pepino fruit contains compounds such as alkaloids, flavonoids, vitamin C which can be efficacious as a hepatoprotector. This study aims to study the activity of the ethanol hepatoprotector extract of pepino fruit (Solanum muricatum) against male white rats induced by ccl4 with extract extract into three groups namely 100 mg / kg, 200 mg / kg and 400 mg / kg. This research is an experimental type using a true experimental design research design. Testing of hepatoprotector activity with reference parameters is SGOT and SGPT levels. Analysis of the results of the SGOT and SGPT measurements were calculated using one-way ANOVA. The results of the measurement of SGPT and SGOT levels with a dose of 100 mg / kgBW, 200 mg / kgBW and 400 mg / kgBW were different. 200 mg / kgBB then pepino fruit ethanol extract group 400 mg / kgBB then the positive group and finally the negative group. pepino with a concentration of 200 mg / kg, then a concentration of 400 mg / kg and finally the negative control group. Based on research that has been done, it can be concluded that the pepino fruit (Solanum muricatum) has hepatoprotector activity at a concentration of 100 mg / kgBW at SGPT and SGOT levels. Keywords : CCL4, Hepatoprotektor, Solanum muricatum.

scholarly journals HEPATOPROTECTIVE EFFECT OF 50% ETHANOL EXTRACT OF SEAGRASS RHIZOME (CYMODOCEA ROTUNDATA) AGAINST LIVER TISSUES IN PARACETAMOL-INDUCED RATS

2018 ◽  
Vol 10 (1) ◽  
pp. 240
Author(s):  
Renita Dewi ◽  
Raesha Dwina Malika ◽  
Ade Mara Meilani ◽  
Fadlina Chany Saputri
Keyword(s):  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Hepatoprotective Effect ◽  
Control Group ◽  
Group Iii ◽  
White Rats ◽  
Cymodocea Rotundata

Objective: This study aimed to determine the hepatoprotective effect of 50% ethanol extract of seagrass rhizome in terms of serum glutamicoxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) activities in paracetamol-induced rat plasma.Methods: This study included 28 male, white rats randomly divided into seven groups. Groups I and II represented the normal control and controlgroups, respectively, administered with 280 mg/kg BW of rhizome extract. Group III represented the negative control group induced by a suspensionof paracetamol (2g/kg BW). Group IV represented a positive control group administered with Hepa-Q® at a dosage of 150 mg/kg BW. Groups V, VI,and VII were administered with seagrass rhizome extract at doses of 140, 280, and 560 mg/kg BW, respectively, before paracetamol induction. Thetest material was orally administered for 17 days. On days 12–17, the rats were induced with paracetamol through the same route. On day 18, bloodsampling was performed followed by SGOT and SGPT plasma measurements.Results: Our results revealed that seagrass rhizome extracts could significantly decrease SGPT and SGOT levels in paracetamol-induced rats (p<0.05)compared with those in the negative control group.Conclusion: Thus, seagrass rhizome extracts possess the potential for development as a hepatoprotective agent.

scholarly journals AKTIVITAS ANTI-INFLAMASI EKSTRAK ETANOL TEPUNG PELEPAH AREN (Arenga pinnanta)

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Devy Angreani M ◽  
Meiske Sangi ◽  
Feti Fatimah
Keyword(s):  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Control Group ◽  
Treatment Groups ◽  
White Rats ◽  
Wistar Strain ◽  
Anti Inflammatory

Tepung pelepah aren secara tradisional digunakan sebagai obat untuk menghilangkan rasa gatal dan luka bakar pada kulit. Penelitian ini bertujuan untuk menguji aktivitas anti-inflamasi ekstrak etanol tepung pelepah aren (Arenga pinnanta) menggunakan metode induksi karagenan. Ekstrak etanol tepung pelepah aren yang digunakan didapatkan dari hasil maserasi tepung pelepah aren menggunakan etanol hasil redestilasi cap tikus. Metode pengujian aktivitas anti-inflamasi menggunakan 15 ekor tikus putih jantan galur wistar dengan berat 150-200 gram yang dibagi dalam 5 kelompok perlakuan, Kelompok Kontrol negative, Kontrol Positif dan kelompok dosis ekstrak etanol 20%, 25% dan 30%. Hasil penelitian ini menunjukkan kelompok ekstrak etanol tepung pelepah aren dosis 30% memiliki kemampuan inhibisi udem yang sama dengan kontrol positif (Na Diklofenak) dan memiliki kemampuan inhibisi yang lebih besar dibandingkan dosis 20% dan 25%.ABSTRACTPalm stem flour is traditionally used as a remedy for the relief of itching and burns on the skin. This research aims to test the anti-inflammatory activity of palm-flour (Arenga pinnanta) ethanol extract using Caragenan induction method. Palm-dried stem flour extract used from the maceration of palm flour, using ethanol redestilation of rat seals. The method of testing anti-inflammatory activity using 15 male white rats with Wistar strain with a weight of 150-200 grams divided into 5 treatment groups, negative control group, positive control and group dose of ethanol extract 20%, 25% and 30%. The results of this study showed that the group of dried palm flour extract dose 30% have the same inhibition capability with positive control (Na Diklofenak) and have greater inhibition capability compared to the dose 20% and 25%.

scholarly journals NEPHROPROTECTIVE ACTIVITY OF ETHANOL EXTRACT ROOT OF COGON GRASS (IMPERATA CYLINDRICA L. (BEAUV.)) ON CREATININE, UREA LEVELS, AND HEMATOLOGY PROFILE AGAINST GENTAMYCIN-INDUCED RENAL TOXICITY IN RATS

2018 ◽  
Vol 11 (13) ◽  
pp. 97
Author(s):  
Aninditha Rachmah Ramadhiani ◽  
Urip Harahap ◽  
Aminah Dalmunthe
Keyword(s):  
Serum Creatinine ◽  
Hematological Parameters ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Normal Group ◽  
Imperata Cylindrica ◽  
Control Group ◽  
Treatment Groups ◽  
Nephroprotective Activity

 Objective: This study aimed to evaluate the effect of ethanol extract root of cogon grass on parameter creatinine, urea levels, and hematology profile in rats induced by gentamicin.Methods: Thirty rats were divided into 6 groups of normal group (without treatment), groups of treatment only fed and watered, given carboxymethylcellulose (CMC) 0.5%, ethanol extract of the roots of cogon grass with each dose of 100, 200, and 400 mg/kg BW. Each group was given the test preparation orally for 8 days. After 1 h, 5 groups were induced by gentamicin at a dose 100 mg/kg bw intraperitoneally during 8 days. On the 9th day, blood samples were collected for serum creatinine, urea levels, and hematological parameters; hemoglobin (Hb), red blood cell, and packed cell volume.Results: Ethanol extract root of cogon grass doses of 100 mg/kg bw, 200 mg/kg bw, and 400 mg/kg BW significantly decreased serum creatinine and urea levels, significantly increased hematological parameters as compared to negative control group of CMC 0.5% and negative control group not given CMC 0.5% (p<0.05). Ethanol extract root of cogon grass at a dose of 400 mg/kg bw showed there were not differences of creatinine, urea levels, and profile of hematology compared with normal group (p>0.05).Conclusion: The ethanol extract root of cogon grass at a dose of 400 mg/kg bw was effective to ameliorate creatinine, urea levels, and hematological parameters.

scholarly journals The Effect of Ethanolic Extract of Roselle (Hibiscuss sabdariffa L) on Increasing SOD Activity

2016 ◽  
Vol 5 (1) ◽  
pp. 118
Author(s):  
Abdul Rahman W ◽  
Nurkhasanah Nurkhasanah ◽  
Nanik Sulistyani
Keyword(s):  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Sod Activity ◽  
Activity Data ◽  
Group Iii

Free radicals were reactive species caused oxidation of lipids membrane and DNA. Superoxide dismutase (SOD) is one of the primary antioxidants to inhibit free radicals. The purpose of this study was to determine the effect of ethanol extract of roselle calyx on SOD enzyme activity of the Sprague Dawley (SD) rats induced by 7.12-dimethylbenzen[a]anthracene (DMBA). The animals age of four weeks divided in to five groups, groups I were normal group. Group II is the negative control group only induced by DMBA 75 mg/kgBW; and group III ,IV , and V were treatment groups that treated by ethanolic extract of roselle calyx at dose of 10, 50 and 100 mg/kgBW/day for 7 days. After treatment with roselle, animals were induced by DMBA 75 mg/kgBW. On day 8 after DMBA induction, animals were fasted for 16 hours and blood was collected to measured SOD activity. Data were analyzed using One Way Analysis Of Variance (ANOVA) and LSD (P &lt;0.05). The result showed the significancies on increasing of SOD activity at 10 mg/kgBW (50.87±1.98), 50 mg/kgBW (69.98±3.58) and 100 mg/kgBW (73.01±6.95) compared with DMBA (43.74±1.95) (p&lt;0,05).

The Effect of Ethanol Extract from Mangosteen Pericarps towards Parasitemia in Plasmodium berghei-Inoculated Mice

2016 ◽  
Vol 1 (4) ◽  
Author(s):  
Debora R. Marito ◽  
Susy Tjahjani ◽  
Khie Khiong
Keyword(s):  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Free Radical Scavenging ◽  
Control Group ◽  
Parasitemia Level

Mangosteen pericarps contains fenolic antioxidant, such as xanthone that acts as free radical scavenging substances and preventing heme polymerization. In this research we evaluated the effect of ethanolic extract of mangosteen pericarps on the parasitemia in Plasmodium berghei-inoculated mice and compared its antimalarial activity with artemisinin monotherapy in reducing the parasitemia in Plasmodium berghei-inoculated mice. Deutschland Denken Yoken (DDY)  mice were randomly divided into 5 groups and inoculated by Plasmodium berghei and given 0.1 mL aquadest (KN), 0.1 mg of artemisinin (KP), 2.5 mg (E1), 0.5 mg (E2) and 0,1 mg (E3) of ethanolic extract from mangosteen pericarps in 0.1 mL aquadest in 3 days. The parasitemia was observed on one day before the treatment, namely on the first day and on the day after the last treatment. We found a highly significant decrease highly significant decrease of the parasitemia in each treatment group compared to the Negative Control group (p < 0.01), and the decrease of parasitemia level in E1 group is similar to the artemisinin monotherapy group (p < 0.05).  Keywords: ethanol extract of mangosteen pericarps, artemisinin, Plasmodium berghei, malaria

scholarly journals HEPATOPROTECTIVE ACTIVITY FROM ETHANOL EXTRACT OF PUGUN TANO’S LEAVE (Curanga fel-terrae (Lour.) Merr.)

2017 ◽  
Vol 10 (11) ◽  
pp. 78
Author(s):  
Urip Harahap ◽  
Marianne Bastian ◽  
Sri Yuliasmi ◽  
Dadang Irfan Husori ◽  
Popi Patilaya ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Negative Control Group ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Negative Control ◽  
Normal Group ◽  
Control Group ◽  
High Dose

Objective: The aim of this study is to observe the activity of ethanol extract of Curanga fel-terrae leave in preventing the damaged of liver which is induced by high dose of paracetamol. Methods: This research was conducted by using Wistar rat divided into 6 groups. Group 1 was the normal group. Group 2, 3, 4, 5 and 6 received CMC-Na 0.5% (negative control), C. fel-terrae ethanolic extract (CFEE) at the doses of 125, 250 and 500 mg/kg, catechin 2 mg/kg (positive control),respectively during 7 days continued and followed by given paracetamol dose of 2.5g/kg 8 hours after that. Hepatoprotective activity was carried out toward parameter of AST, ALT as well as histopathology of the liver. Results: The results showed that high-dose paracetamol dose of 2.5g/kg bw can cause liver damaged which can be seen by the increasing of the level of AST and ALT compared to the normal group (p<0.05). The usage of three doses of CFEEfor 7 days showed the prevention of the increasing of the level of AST and ALT compared to negative control group (p<0.05). Furthermore, the histopathology study revealed that the three doses of extract could protect the liver. Conclusion: The C. fel-terrae ethanolic extract (CFEE) at the doses of 125, 250 and 500 mg/kg bw which was given for 7 days can prevent the liver from the damage caused by high-dose of paracetamol. Keyword: Curanga fel-terrae (Lour.) Merr., paracetamol, liver, hepatoprotective   

scholarly journals The Effect of Ethanolic Extract of Roselle (Hibiscuss sabdariffa L) on Increasing SOD Activity

2016 ◽  
Vol 5 (1) ◽  
pp. 118
Author(s):  
Abdul Rahman W ◽  
Nurkhasanah Nurkhasanah ◽  
Nanik Sulistyani
Keyword(s):  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Sod Activity ◽  
Activity Data ◽  
Group Iii

Free radicals were reactive species caused oxidation of lipids membrane and DNA. Superoxide dismutase (SOD) is one of the primary antioxidants to inhibit free radicals. The purpose of this study was to determine the effect of ethanol extract of roselle calyx on SOD enzyme activity of the Sprague Dawley (SD) rats induced by 7.12-dimethylbenzen[a]anthracene (DMBA). The animals age of four weeks divided in to five groups, groups I were normal group. Group II is the negative control group only induced by DMBA 75 mg/kgBW; and group III ,IV ,and V were treatment groups that treated by ethanolic extract of roselle calyx at dose of 10, 50 and 100 mg/kgBW/day for 7 days. After treatment with roselle, animals were induced by DMBA 75 mg/kgBW. On day 8 after DMBA induction, animals were fasted for 16 hours and blood was collected to measured SOD activity. Data were analyzed using One Way Analysis Of Variance (ANOVA) and LSD (P &lt;0.05). The result showed the significancies on increasing of SOD activity at 10 mg/kgBW (50.87±1.98), 50 mg/kgBW (69.98±3.58) and 100 mg/kgBW (73.01±6.95) compared with DMBA (43.74±1.95) (p&lt;0,05).

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

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