scholarly journals Anti-urolithiatic Activity of Sambong (Blumea balsamifera) Extract in Ethylene Glycol-induced Urolithiatic Wistar Rats (Rattus norvegicus)

2020 ◽  
Vol 54 (1) ◽  
Author(s):  
Althea Samantha C. Agdamag ◽  
Larielyn Hope C. Aggabao ◽  
Mary Sheena C. Agudo ◽  
Francis Louis M. Alcachupas ◽  
Jeremiah Carlo V. Alejo ◽  
...  
Keyword(s):  
Treatment Group ◽  
Ethylene Glycol ◽  
Calcium Oxalate ◽  
Stone Formation ◽  
Positive Control ◽  
Control Group ◽  
Control Group Design ◽  
Calcium Oxalate Stone ◽  
Kidney Homogenate ◽  
Calcium Oxalate Stones

Objective. The study aimed to determine if Blumea balsamifera inhibits calcium oxalate stone formation in the kidneys through determination of the number of calcium oxalate stones in the renal cortex and the percent mass of calcium oxalate. Methods. Post-test only control group design was used using five treatment groups with placebo as the negative control, potassium citrate as the positive control, and 50%, 100%, and 200% sambong treatment. Urolithiasis was induced through ethylene glycol and ammonium chloride. Each treatment group was administered its corresponding treatment solution once daily for twenty-one days. Histopathologic examination and kidney homogenate analysis were done to determine the degree of deposition of calcium oxalate stones in renal tissues and the oxalate content, respectively. Statistical analyses were performed using one-way ANOVA and post hoc Gabriel's Pairwise Comparisons Test. Results. The 100% sambong treatment group showed the least mean number of stones while the positive control and 50% sambong treatment group exhibited the highest anti-urolithiatic activity in terms of oxalate content of the kidney homogenate. Conclusion. It can be concluded from the study that Blumea balsamifera inhibits calcium oxalate stone formation in the kidneys with the 100% and 50% sambong treatment most effective in decreasing number of stones and oxalate content of the kidney homogenate, respectively.

scholarly journals Potensi Ekstrak Daun Kelor (Moringa Oleifera) sebagai Hepatoprotektor pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Dosis toksik

2018 ◽  
Vol 5 (1) ◽  
pp. 58
Author(s):  
Noer Kumala Indahsari ◽  
Masfufatun Masfufatun ◽  
Emilia Devi D.R
Keyword(s):  
Treatment Group ◽  
Moringa Oleifera ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
High Dose ◽  
Control Group Design ◽  
Post Test

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals Pengaruh Pemberian Aspartam terhadap Kadar Low-Density Lipoprotein dan High-Density Lipoprotein pada Tikus Wistar Diabetes Melitus Diinduksi Aloksan

2014 ◽  
Vol 3 (3) ◽  
Author(s):  
Revivo Rinda Pratama ◽  
Eti Yerizel ◽  
Rahmatini Rahmatini
Keyword(s):  
Diabetes Mellitus ◽  
Treatment Group ◽  
Daily Intake ◽  
Density Lipoprotein ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Diabetes Melitus

AbstrakAspartam telah disetujui oleh FDA untuk dikonsumsi. Konsumsi pemanis buatan ini menggunakan dosis ADI (Acceptable Daily Intake) yaitu 50 mg/kgBB. Individu dengan diabetes melitus kemungkinan menjadi antusias terhadap adanya aspartam. Aspartam dapat mempengaruhi metabolisme profil lipid. Tujuan dari penelitian ini adalah mengetahui pengaruh pemberian aspartam terhadap kadar LDL dan HDL tikus diabetes melitus diinduksi aloksan. Ini merupakan penelitian eksperimental dengan randomized post test only control group design. Subjek penelitian adalah 15 ekor tikus Wistar jantan yang dibagi menjadi tiga kelompok, yaitu kelompok kontrol negatif, kelompok kontrol positif, dan kelompok perlakuan. Masing-masing kelompok terdiri dari lima (5) ekor tikus. Pemberian aspartam (dosis 315 mg/kgBB tikus) diberikan kepada kelompok perlakuan selama empat (4) minggu. Hasil penelitian menunjukkan bahwa pemberian aspartam pada tikus diabetes melitus diinduksi aloksan berpengaruh terhadap penurunan kadar LDL dan peningkatan kadar HDL. Kadar LDL pada kelompok kontrol positif adalah 30 ± 2 mg/dl, pada kelompok perlakuan adalah 24 ± 2 mg/dl. Sedangkan kadar HDL pada kelompok kontrol positif adalah 19 ± 1 mg/dl, pada kelompok perlakuan adalah 22 ± 1 mg/dl. Terdapat perbedaan yang bermakna pada kadar LDL dan HDL antara kelompok kontrol positif dengan kelompok perlakuan. Kesimpulan hasil penelitian ini adalah pemberian aspartam pada tikus diabetes melitus diinduksi aloksan berpengaruh terhadap penurunan kadar LDL dan peningkatan kadar HDL.Kata kunci: aspartam, diabetes melitus, LDL, HDLAbstractAspartame has been approved by the FDA for consumption. Consumption of artificial sweeteners is using ADI (Acceptable Daily Intake) dose which is 50 mg/kg. Individuals with diabetes mellitus would likely be enthusiastic consumers of aspartame. Aspartame can influence the metabolism of lipid profile. The purpose of this study was to determine the effect of aspartame on levels of LDL and HDL at rats with diabetes mellitus induced by alloxan. The research is experimental research with randomized post test only control group design. The subjects were 15 male Wistar rats those were divided into three groups: a negative control group, a positive control group, and the treated group. Each group consists of five (5) male rats. Administration of aspartame (dose of 315 mg/kg rat) was administered to the treatment group for four (4) weeks. The results showed that administration of aspartame in rats with diabetes mellitus induced by alloxan was influenced the decreased in LDL levels and increased in HDL levels. The LDL levels in positive control group was 30 ± 2 mg/dl, in the treatment group was 24 ± 2 mg/dl. While the levels of HDL in positive control group was 19 ± 1 mg/dl, in the treatment group was 22 ± 1 mg/dl. There is a significant difference in the levels of LDL and HDL between the positive control group with the treatment group. The conclusion of this research are the administration of aspartame in rats with diabetes mellitus induced by alloxan influenced the decreased in LDL levels and increased in HDL levels.Keywords: aspartame, diabetes mellitus, LDL, HDL

scholarly journals POTENSI EKSTRAK ETANOL BAWANG MERAH (Allium ascolonicum L.) DAN GARAM NaCl MENURUNKAN LUAS AREA SERTA MENINGKATKAN KONTRAKSI JARINGAN LUKA BAKAR RINGAN

Jurnal Kimia ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 53
Author(s):  
I M. Sukadana ◽  
S. Rahayu ◽  
Melli Melli
Keyword(s):  
Treatment Group ◽  
Subcutaneous Tissue ◽  
Healing Process ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Skin Damage ◽  
Control Group Design ◽  
Onion Extract ◽  
Skin Response

Burns is a skin response and subcutaneous tissue to temperature or thermal trauma that affects skin damage. The weight or severity of tissue damage from burns is influenced by several factors including deep burns, burns area, burn location, general body health, injury mechanism and age. The area of ??burns and the percentage of wound contractions is an indicator of the healing process of minor burns. This study used 25 wistar rats divided into 5 treatment groups with randomized posttest only control group design as follows; K: given vaseline treatment (negative control), Kp: given bioplacenton treatment (positive control), P1: given salt treatment at 7.5% concentration in vaseline, P2: given onion extract treatment at 40% concentration in vaseline, and P3: given a mixture of onion extract 40% and kitchen salt at a concentration of 7,5% in vaseline. The result of One-Way ANOVA and Post Hoc Test LSD using SPSS for Windows version 19 shows P2 treatment group is onion extract of 40% give the best result concentration of onion extract so it is very potential to be developed further as light burning agent compared to P1 and P3 treatment group because of its ability to decrease the area of ??340,79 mm2 on the first day to 11,75 mm2 and increased wound contraction by 81,59% at the end of treatment

scholarly journals Aqueous extract of Parkinsonia aculeata has an anti-urolithiatic effect in rats

2021 ◽  
Author(s):  
Seyed Sajjad Eghbali ◽  
Samad Akbarzadeh ◽  
Afshar Bargahi ◽  
Kamran Mirzaei ◽  
Nasrollah Maleki ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Calcium Oxalate ◽  
Low Dose ◽  
Renal Calculi ◽  
Control Group ◽  
High Dose ◽  
Renal Tubules ◽  
Distilled Water ◽  
Moderate Dose ◽  
Calcium Oxalate Stones

Abstract Introduction: Many antioxidant agents have been studied for prevention and treatment of the kidney stone disease in animals and humans. Parkinsonia aculeate (P. aculeate) has a wide range of pharmacological and biological activities, particularly antioxidant properties. The goal of this research was to investigate the anti-urolithiatic effect of P. aculeate extract on ethylene glycol induced renal calculi.Materials and methods: A total of 42 male Wistar rats were randomly divided into six equal groups with seven rats in each group: Group A served as healthy control group and received distilled water for 30 days. Group B served as ethylene glycol control group and received 1% v/v ethylene glycol in distilled water for 30 days. Group C, D, and E animals (preventive groups) concurrently received 1% v/v ethylene glycol in distilled water along with extract of P. aculeata orally for 30 days in doses of 100 mg/kg (low dose), 200 mg/kg (moderate dose), and 300 mg/kg (high dose), respectively. Group F (treatment groups) received 1% v/v ethylene glycol in distilled water for the first 30 days along with extract of P. aculeata 300 mg/kg orally from 15th to 30th days. At the end of study period, 24-hour urine and serum samples were collected and analyzed. All rats were sacrificed and both kidneys were removed, weighed and histopathologically evaluated for calcium oxalate crystals.Results: Ethylene glycol significantly decreased serum calcium, urinary levels of creatinine, calcium and uric acid. On the contrary, it significantly increased 24-hour urine volume, kidney weight, and calcium oxalate deposits. The highest number of calcium oxalate crystal depositions were in the high dose preventive group (76.6%), which is statistically significant difference with other groups including ethylene glycol control (70%), low dose preventive (72%), treatment (38%), and moderate dose preventive (42%) groups (P value < 0.05). Administration of extract of P. aculeata significantly reduced the production of calcium oxalate stones in moderate dose preventive and treatment group. However, P. Aculeata extract was not effective in reducing the formation of calcium oxalate stones in low dose and high dose preventive groups. Interestingly, the number of calcium oxalate deposits in the renal tubules of high dose preventive group was significantly more than the ethylene glycol control group.Conclusions: Extract of P. aculeata has preventive and therapeutic effects in ethylene glycol induced renal calculi in Wistar rat. Further studies are necessary to clarify the mechanism underlying this effect.

Testosterone Enhances whereas Estrogen Inhibits Calcium Oxalate Stone Formation in Ethylene Glycol Treated Rats

1996 ◽  
Vol 156 (2) ◽  
pp. 502-505 ◽  
Author(s):  
Ying Huei Lee ◽  
Wann Chu Huang ◽  
Jong Khing Huang ◽  
Luke S. Chang
Keyword(s):  
Ethylene Glycol ◽  
Calcium Oxalate ◽  
Stone Formation ◽  
Calcium Oxalate Stone

scholarly journals Effect of Chitosan on Histology of Reproductive Organs of Female Wistar Rats (Rattus norvegicus) Exposed to Acetate Lead

2019 ◽  
Vol 30 (4) ◽  
pp. 259
Author(s):  
Alief Ayu Purwitasari ◽  
Annisa' Wigati Rozifa ◽  
Dwi Dianita Irawan ◽  
Umi Kalsum ◽  
Retty Ratnawati ◽  
...  
Keyword(s):  
Treatment Group ◽  
Lead Acetate ◽  
Antral Follicle ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Female Rats ◽  
Control Group ◽  
P Value ◽  
Control Group Design

Lead is one of the pollutants widely spread in the environment because it is not easily decomposed. Lead can affect system functions such as the ovary and endometrium. Lead can trigger oxidative stress by reducing antioxidant enzymes and increasing Reactive Oxygen Species (ROS). Lead can also reduce Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) levels by disturbing the hypothalamus. Chitosan is an antioxidant compound that can reduce the toxic effects of lead. The purpose of this study was to study the effects of chitosan administration on the diameter of antral follicles, the number of endometrial arterioles, and the thickness of endometrial rats after lead acetate exposure. This study was an experimental laboratory using a posttest-only control group design approach applied on 25 female rats aged 8 weeks old, body weight 125-175 grams. Lead and chitosan were given orally with a sonde. There were 5 groups, namely, negative control group (without any treatment), positive control group (lead 175mg/kg/BW), treatment group 1 (lead 175mg/kg/BW + chitosan 16mg/kg/BW, treatment group 2 (lead 175mg/kg/BW + chitosan 32mg/kg/BW), and treatment group 3 (lead 175mg/kg/BW + chitosan 64mg/kg/BW) for 30 days. The rats were sacrificed at proestrus phase, which was proven from vaginal swab. Observations were carried out using the Hematoxylin Eosin (HE) staining method. The observations were analyzed using One Way ANOVA and followed by Least Significant Differences (LSD) test. The results showed significant results (p-value &lt;0.05). Chitosan can increase the diameter of the antral follicle, increase the number of endometrial arterioles, and increase the thickness of endometrial rats exposed by lead acetate.

Utilization of Tofu Dregs Flour in Making Snack Bars for People with Diabetes Mellitus

2020 ◽  
Vol 7 (1) ◽  
pp. 27
Author(s):  
Ni Putu Diah Cahyani Subamia ◽  
Komang Ayu Nocianitri ◽  
I Dewa Gede Mayun Permana
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Wheat Flour ◽  
Sugar Content ◽  
Type Ii Diabetes Mellitus ◽  
Positive Control ◽  
Water Soluble ◽  
Control Group ◽  
Unhealthy Lifestyle ◽  
Control Group Design

Type II diabetes mellitus due to an unhealthy lifestyle, one of which is the lack of fiber in daily food consumption. One food that has a high fiber content is tofu dregs. The purpose of this study was to determine substitution of wheat flour with tofu dregs flour to produce a snack bar with the best characteristics, and determine the effect of consumption of snack bar from tofu dregs on blood sugar content in rats. The research was conducted two steps. Step I: Formulation of snack bar using a completely randomized design with tofu dregs flour concentration of 0 %, 10 %, 20 %, 30 %, 40 %, and 50 %. The variables of this study were the content of water, ash, protein, fat, carbohydrates, sensory tests, and effectiveness tests. Step II: the best characteristic snack bar in the step I was used experimental rats. This step used true experimental design with pre-post test control group design. The variables of study were blood glucose levels before treatment and after treatment. The treatment group consisted of normal, negative, positive control, and snack bar. The results of the first step of the research showed that substitution of wheat flour with 40 % tofu dregs produced the best characteristic snack bar with 17.19 % water content, 1.33 % ash content, 11.03 % protein, 20.53 % fat, 49.92 % carbohydrate, light brown color, unpleasant aroma, distinctive soy taste, crumb texture, 0.63 % water soluble food fiber, 1.57 % water insoluble fiber, and total food fiber 2.36 %. The results of the second step of the study showed that the provision of substitution of wheat flour with tofu dregs flour 40 % could reduce blood sugar levels in diabetic mellitus rats until normal, start 290 mg/dl to 108.5 mg/dl. Tofu dregs flour can be used for snack bar formulations for people with diabetes mellitus.

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