scholarly journals THIẾT LẬP MẪU MÁU GIẢ ĐỊNH CHỨA VI KHUẨN STAPHYLOCOCCUS AUREUS DÙNG TRONG NGOẠI KIỂM

2021 ◽  
Vol 500 (1) ◽  
Author(s):  
Hà Mạnh Tuấn ◽  
Nguyễn Thị Thu Diểm
Keyword(s):  
Staphylococcus Aureus ◽  
Nutrient Broth ◽  
Mau Mau

Mục tiêu: Thiết lập mẫu máu giả định chứa S. aureus  sử dụng cho chương trình ngoại kiểm chất lượng vi sinh đạt độ đồng nhất và ổn định theo ISO 17043:2011. Phương pháp: Nghiên cứu thực nghiệm. Đánh giá tốc độ tăng trưởng của và S. aureus trong môi trường máu giả định có và không có acid boric và natriformat. Xác định nồng độ acid boric và natriformat phù hợp. Thử nghiệm sản xuất bộ mẫu máu giả định chứa cơ chất nutrient broth, máu cừu, acid boric và natrifomat theo nồng độ đã xác định. Sử dụng phép kiểm T-Student và Oneway ANOVA đánh giá tính đồng nhất, tính ổn định bộ mẫu máu giả định đã sản xuất. Kết quả: Sử dụng acid boric và natrifomat duy trì nồng độ S. aureus trong mẫu (máu cứu, nutrient broth) hiệu quả hơn so với không sử dụng. Nồng độ acid boric 8% và natriformat 4% là phù hợp với sản xuất mẫu máu giả định vi khuẩn đích là S.aureus. Bộ mẫu sản xuất chứa vi khuẩn đích S. aureus đạt tính đồng nhất với Fthực nghiệm= 0,911 < F lý thuyết =3,02, ổn định trong 17 ngày. Kết luận: Qua nghiên cứu, sử dụng acid boric- natrifomat làm chất bảo quản trong môi trường máu giả định là phù hợp. Thiết lập thành công bộ mẫu máu giả định S. aureus sử dụng cho chương trình ngoại kiểm vi sinh theo tiêu chuẩn ISO 17043:2011 đạt tính đồng nhất và tính ổn định trong 17 ngày ở 220C-300C.

scholarly journals 17. Oxytetracycline Activities To Staphylococcus aureus Biofilm Inhibition Of Aceh Cattle Preputium Isolate

2019 ◽  
Vol 13 (1) ◽  
pp. 125-131
Author(s):  
Maryulia Dewi ◽  
Darmawi Darmawi ◽  
T. Zahrial Helmi ◽  
Erina Erina ◽  
Basri Gani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Optical Density ◽  
Antimicrobial Activities ◽  
Nutrient Broth ◽  
Biofilm Inhibition ◽  
Inhibitory Capacity ◽  
Inhibitory Power

The aim of this study was to see the antimicrobial activities of oxytetracycline to the inhibition of Staphylococcus aureus biofilm of Aceh’s cattle preputium isolates. The parameters measured in this study were the percentage of antimicrobial and biofilm inhibitory power. The antimicrobial activities were tested on Nutrient Broth using U-bottom polystyrene microplate 96 wells. The Biofilm stained using 1% of crystalviolet. Then, Optical Density (OD) read at λ 595 nm. The results showed that the oxytetracycline of 10%, 20% and 30% (μg / μL) had the antimicrobial abilities of 28.60% ± 13.23%; 36.30% ± 5.37%; and 57.70% ± 2.23%. In addition, The biofilm inhibitory capacity of oxytetracycline 10%, 20% and 30% (μg / μL) were 2.20% ± 8.77%; 2.70% ± 4.17%; and 3.73% ± 14.14%. Therefore, it can be concluded that the oxytetracycline  had the ability to inhibit the biofilm of Staphylococcus aureus.

scholarly journals IDENTIFIKASI BAKTERI Staphylococcus aureus PADA PREPUTIUM DAN VAGINA KUDA (Equus caballus) (Identification of Staphylococcus aureus in Preputium and Vagina of Horses (Equus caballus))

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Yulina Rahmi ◽  
Darmawi Darmawi ◽  
Mahdi Abrar ◽  
Faisal Jamin ◽  
Fakhrurrazi Fakhrurrazi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Equus Caballus ◽  
Nutrient Broth ◽  
Gram Positive ◽  
Gram Staining ◽  
Bright Yellow ◽  
Reaction Tube ◽  
The Media ◽  
The Village

This research aims to identify the Staphylococcus aureus (S. aureus) bacteria from preputium and vagina of horses. Five swabs preputium and 5 swabs vagina samples from 3-9 years olds horse in the village of Bebangka, Gelelungi, Reloop, and Kelapeng, Pegasing Aceh Tengah District were used in this study. Preputium and vagina swabs of horses were dipped into reaction tube containing Nutrient Broth (NB). Staphylococcus aureus was identified on manitol salt agar (MSA). Separated colony colored bright yellow on the media were tested for Gram staining, hemolysis, catalase, and sugar test (glucose and manitol). The result showed that the isolates growth on MSA were colored bright yellow, Gram positive, β hemolisis, and catalase positive. The isolates fermented manitol and glucose. Isolates identified from horses preputium and vagina showed the characteristic of S. aureus. In conclusion S. aureus bacteria were positively identified from all of swabs samples, both in preputium and vagina of horses.Key words: Staphylococcus aureus, horse, preputium, vagina

scholarly journals Antimicrbial Effect of Diode Laser and Biossynthesis Silver Nanoparticles on Bactrium Staphylococcus Aureus in Vitro

2018 ◽  
Vol 7 (4.36) ◽  
pp. 290
Author(s):  
Raad Sh. Alnayli ◽  
Adnan H. Al Hamadani ◽  
Safaa A. Yaseen
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Diode Laser ◽  
Bacterial Growth ◽  
Antibacterial Agent ◽  
Laser Energy ◽  
Nutrient Broth ◽  
Biological Method ◽  
Effective Result

The present work was aimed to illustrate how the use of Diode laser or silver nanoparticles (AgNPs) each of them individually, as an antibacterial agent against  Staphylococcus aureus (S. aureus) as well as study the effective result from the absorption of laser energy by these nanoparticles to kill or inhibition bacterial growth. The silver nanoparticles are prepared by biological method. S. aureus are isolated and identified in the Central Health Laboratory in Al Najaf city. Nanoparticles are tested against S. aureus cultured on Muller Hinton agar but Diode laser or laser with nanoparticles are tested against S. aureus cultured in Nutrient broth. S. aureus are irradiating by Diode laser with different irradiation times, moreover there are different concentrations of AgNPs have been employed for killing and inhibition bacterial growth. Results showed that S. aureus is affected by AgNPs (mainly highest concentration of nanoparticles) also detected that decrease bacterial availability with increase time of irradiation to laser. 

Turbidimetric Evaluation of Bacterial Culture Resistance in Disinfectant Testing

1972 ◽  
Vol 55 (1) ◽  
pp. 219-223
Author(s):  
John H S Chen ◽  
L F Ortenzio
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacterial Growth ◽  
Bacterial Culture ◽  
Nutrient Broth ◽  
Turbidimetric Method ◽  
Salmonella Choleraesuis ◽  
Test Organisms ◽  
Test Cultures

Abstract A simple turbidimetric method has been developed for determining the degree of phenol resistance of cultures used for disinfectant testing. Net transmission values are used to assess t h e degree of resistance; these are based on t h e measurement of the total amount of light scattered by the bacterial growth i n the AOAC broth medium without interfering turbidity of the dissolved ingredients. A decrease in the net transmission value corresponds to a decrease in phenol resistance; the converse is also true . The transmission values obtained in a 10 month study of a total of 77 weekly r and om samples of AOAC nutrient broth media blanks showed no substantial differences among the broth media tested by the turbidimetric method. Net transmission values were obtained for 71 broth cultures of Salmonella choleraesuis, 74 of Staphylococcus aureus, and 78 of Pseudomonas aeruginosa having the same required resistance against phenol. These values, which fell within a wider range than would be expected, are given. The effect of the number of 10-carrier-soakings on the net transmission value and equivalent phenol resistance of the cultures was determined for 60 broth cultures of the 3 test organisms named above. Soaking wet cylinder carriers in a given volume of test cultures used for the AOAC use-dilution test directly reduced the net transmission value and equivalently decreased the phenol resistance of the cultures. The turbidimetric method has proven experimentally satisfactory i n several hundred determinations and in selecting test cultures of uniform quality for disinfectant testing.

Survival of Staphylococcus aureus and Escherichia coli as Affected by Ethanol and NaCl

2001 ◽  
Vol 64 (4) ◽  
pp. 546-550 ◽  
Author(s):  
SUE-LANG HUANG ◽  
YIH-MING WENG ◽  
ROBIN Y.-Y. CHIOU
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Concentration ◽  
Inhibitory Effect ◽  
Limited Range ◽  
Nutrient Broth ◽  
Nutrient Agar ◽  
Ethanol Sensitivity ◽  
E Coli ◽  
Viable Cells

Growth of three strains of Staphylococcus aureus and two strains of Escherichia coli on nutrient agar (NA) supplemented with ethanol and NaCl was investigated. S. aureus did not grow on NA containing ≧10% ethanol (wt/wt) combined with ≧0% NaCl (wt/wt), or 7.5% ethanol combined with 7.5% NaCl. Neither E. coli nor E. coli O157:H7 grew on NA containing ≧7.5% ethanol combined with ≧0% NaCl, 5% ethanol combined with ≧2.5% NaCl, or ≧5% NaCl combined with ≧0% ethanol. It is apparent that NaCl enhanced the inhibitory effect of ethanol on growth of S. aureus and E. coli. When cells were suspended in nutrient broth containing 12.5, 20, or 40% ethanol combined with NaCl, viable cells decreased with an increase of ethanol concentration. Ethanol sensitivity among strains and between genera varied in a limited range. When the cells were exposed to 20% ethanol in combination with 5% NaCl, S. aureus and E. coli lost viability after 30 and 10 min, respectively. When treated with 40% ethanol combined with ≧0% NaCl, all test strains lost viability within 5 min.

scholarly journals Comparative evaluation of antibacterial activity of silver oxides with different oxygen content

2021 ◽  
pp. 23-28
Author(s):  
E.M. Gordina ◽  
◽  
S.A. Bozhkova ◽  
A.A. Erusin ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Oxygen Content ◽  
Optical Density ◽  
Antibacterial Properties ◽  
Nutrient Broth ◽  
Low Oxygen ◽  
Pure Silver ◽  
And Staphylococcus Aureus ◽  
Density Results

Aim of the study. To study the presence of antibacterial properties in silver oxides with different oxygen content in relation to sensitive and methicillinresistant Staphylococcus aureus strains. Material and Methods. Films of silver and its oxides were coated on paper surface in vacuum via the method of ion-plasma arc sputtering of the metallic target. Antibacterial activity of the obtained samples of silver oxides and pure silver against Staphylococcus aureus АТСС 29213 and Staphylococcus aureus ATCC 43300 was studied applying the diff usion method as well as in liquid nutrient broth with consequent measuring of optical density. Results. As a result of antibacterial activity investigation of the samples obtained via the diff usion method and liquid nutrient broth, it was revealed that all samples possessed antibacterial activity in relation to type strains of S. aureus. Th erewith, the effi cacy of the samples with pure silver was signifi cantly lower than that of silver oxides (Ag0,2O, Ag0,4O, Ag1O, Ag1,5O, Ag2O). It was shown that the activity of silver oxides did not depend on the oxygen concentration in them. Thus, statistically signifi cant diff erences in optical density of the nutrient medium with bacteria and the medium with incubated S. aureus АТСС 29213 (MSSA) and AgxO (р<0.001). Analogical results were obtained aft er studying of infl uence exerted by silver on S. aureus АТСС 43300 (MRSA). Conclusion. All silver oxides samples created were characterised by antibacterial activity against S. aureus. The data obtained point at the prospects in application of silver oxide with low oxygen content for prevention and treatment of Staphylococcus aureus strains infection.

scholarly journals Inactivation of Staphylococcus aureus by the Combined Treatments of Ultrasound and Nisin in Nutrient Broth and Milk

eFood ◽  
2021 ◽  
Author(s):  
YongCheng Song ◽  
Yushuang Lu ◽  
Xiufang Bi ◽  
Liyi Chen ◽  
Lei Liu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Nutrient Broth ◽  
Combined Treatments

Evaluation of a Selective Broth for Detection of Staphylococcus aureus Using Impedance Microbiology

2001 ◽  
Vol 64 (1) ◽  
pp. 44-50 ◽  
Author(s):  
KIRSTEN E. GLASSMOYER ◽  
SCOTT M. RUSSELL
Keyword(s):  
Staphylococcus Aureus ◽  
Nalidixic Acid ◽  
Bacterial Species ◽  
Nutrient Broth ◽  
Microbiological Method ◽  
E Coli ◽  
Poultry Products ◽  
Chicken Carcass ◽  
Low Levels ◽  
Staphylococcus Spp

Experiments were conducted to evaluate a selective nutrient broth containing acriflavine and nalidixic acid for detection of Staphylococcus aureus using an impedance microbiological method. Nine species of bacteria, other than S. aureus, were evaluated using the selective broth to determine if these species could be inhibited. A total of 10 ppm of nalidixic acid inhibited the gram-negative species tested, with the exception of Pseudomonas aeruginosa. Similarly, 10 ppm of acriflavine suppressed the Staphylococcus spp. examined; however, S. aureus retained the ability to proliferate. Nutrient broth solution containing 10 ppm of nalidixic acid and 10 ppm of acriflavine (S. aureus impedance broth [SIB]) inhibited multiplication of most of the bacterial species tested and allowed S. aureus to be detected in an average of 16.4 h. Fresh chicken carcass rinses and cooked chicken rinses were inoculated with Escherichia coli and S. aureus and assayed using SIB in conjunction with impedance. Results demonstrated that S. aureus could be detected in less than 11.5 h, although the presence of E. coli decreased detection times. Additionally, impedance assays were conducted using five different poultry products to evaluate the sensitivity of the broth for detecting S. aureus. S. aureus could be detected on poultry products when present at low levels (101 CFU/ml) in less than 24 h. These studies demonstrated that SIB may be used in conjunction with impedance for rapid detection of S. aureus. However, without further modification, this method should not be used for enumeration of S. aureus from samples containing mixed microflora.

Sign in / Sign up

Export Citation Format

Share Document