17. Oxytetracycline Activities To Staphylococcus aureus Biofilm Inhibition Of Aceh Cattle Preputium Isolate

The aim of this study was to see the antimicrobial activities of oxytetracycline to the inhibition of Staphylococcus aureus biofilm of Aceh’s cattle preputium isolates. The parameters measured in this study were the percentage of antimicrobial and biofilm inhibitory power. The antimicrobial activities were tested on Nutrient Broth using U-bottom polystyrene microplate 96 wells. The Biofilm stained using 1% of crystalviolet. Then, Optical Density (OD) read at λ 595 nm. The results showed that the oxytetracycline of 10%, 20% and 30% (μg / μL) had the antimicrobial abilities of 28.60% ± 13.23%; 36.30% ± 5.37%; and 57.70% ± 2.23%. In addition, The biofilm inhibitory capacity of oxytetracycline 10%, 20% and 30% (μg / μL) were 2.20% ± 8.77%; 2.70% ± 4.17%; and 3.73% ± 14.14%. Therefore, it can be concluded that the oxytetracycline had the ability to inhibit the biofilm of Staphylococcus aureus.

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Comparative evaluation of antibacterial activity of silver oxides with different oxygen content

Siberian medical review ◽

10.20333/25000136-2021-2-23-28 ◽

2021 ◽

pp. 23-28

Author(s):

E.M. Gordina ◽

◽

S.A. Bozhkova ◽

A.A. Erusin ◽

◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Oxygen Content ◽

Optical Density ◽

Antibacterial Properties ◽

Nutrient Broth ◽

Low Oxygen ◽

Pure Silver ◽

And Staphylococcus Aureus ◽

Density Results

Aim of the study. To study the presence of antibacterial properties in silver oxides with different oxygen content in relation to sensitive and methicillinresistant Staphylococcus aureus strains. Material and Methods. Films of silver and its oxides were coated on paper surface in vacuum via the method of ion-plasma arc sputtering of the metallic target. Antibacterial activity of the obtained samples of silver oxides and pure silver against Staphylococcus aureus АТСС 29213 and Staphylococcus aureus ATCC 43300 was studied applying the diff usion method as well as in liquid nutrient broth with consequent measuring of optical density. Results. As a result of antibacterial activity investigation of the samples obtained via the diff usion method and liquid nutrient broth, it was revealed that all samples possessed antibacterial activity in relation to type strains of S. aureus. Th erewith, the effi cacy of the samples with pure silver was signifi cantly lower than that of silver oxides (Ag0,2O, Ag0,4O, Ag1O, Ag1,5O, Ag2O). It was shown that the activity of silver oxides did not depend on the oxygen concentration in them. Thus, statistically signifi cant diff erences in optical density of the nutrient medium with bacteria and the medium with incubated S. aureus АТСС 29213 (MSSA) and AgxO (р<0.001). Analogical results were obtained aft er studying of infl uence exerted by silver on S. aureus АТСС 43300 (MRSA). Conclusion. All silver oxides samples created were characterised by antibacterial activity against S. aureus. The data obtained point at the prospects in application of silver oxide with low oxygen content for prevention and treatment of Staphylococcus aureus strains infection.

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Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus Isolat Preputium Sapi Aceh (Antibiotic Activities To Staphylococcus aureus Biofilms Of Aceh Cattle Preputium Isolate)

Jurnal Sain Veteriner ◽

10.22146/jsv.35219 ◽

2020 ◽

Vol 38 (1) ◽

pp. 1

Author(s):

Maryulia Dewi ◽

Darmawi Darmawi ◽

T Zahrial Helmi

Keyword(s):

Staphylococcus Aureus ◽

Analysis Of Variance ◽

Optical Density ◽

False Positive ◽

Bacterial Virulence ◽

Nutrient Broth ◽

Aureus Isolate ◽

Randomized Design ◽

Completely Randomized Design

AbstractBiofilm is one of parameters for determining pathogenic pathways of a bacterium. Bacterial virulence activities can be attenuated by antibiotics with inhibiting biofilm. The aim of this study was to examine the characteristics and activities of antibiotics against the biofilm of Stapylococcus aureus isolate which taken from Aceh cattle prepuce. The study was exploratory and experimental, using a Completely Randomized Design (RAL) factorial pattern using three types of antibiotics, three concentrations and replications. Antibiotics were tetracycline, oxytetracycline and phosphomycin with concentrations of 10%, 20% and 30% (mg/mL), respectively. Therefore, the aim of this study was to examine the characteristics and activities of antibiotics to the biofilm of Stapylococcus aureus from Aceh cattle prepuce isolate. U-bottom polystyrene 96 wells microplate and Nutrient Broth (NB) medium were used to biofilm. Bacterial while biofilm was stained on crystals violet 1%. Optical Density (OD) of biofilm were λ 595 nm. Data were analyzed by using Analysis Of Variance (ANOVA). The results showed that the activity of Staphylococcus aureus was moderately positive, i.e 2,667cell/μL. It showed that antibiotics and concetrations have significant effect (P<0.05) to inhibit biofilm staphylococcus aureus. In additioon, tetracycline 30% (mg / mL) has the best ability to inhibit biofilms from 52,18% to 1,262 cell/μL. Based on that fact, it can be concluded that the virulence of Staphylococcus aureus was moderate positive to be false positive. Keywords: biofilm, antibiotics, Staphylococcus aureus, cows aceh. Abstrak Biofilm merupakan salah satu parameter untuk menentukan jalur patogenisitas suatu bakteri. Antibiotik dapat melemahkan aktivitas virulensi bakteri dengan menghambat pembentukan biofilm. Penelitian ini bertujuan untuk melihat karaksteristik dan aktivitas antibiotik terhadap biofilm Stapylococcus aureus isolat preputium sapi Aceh. Penelitian bersifat eksplorasi dan eksperimental, menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan menggunakan tiga jenis antibiotik, tiga konsentrasi dan tiga kali ulangan. Antibiotik yang digunakan adalah tetrasiklin, oksitetrasiklin dan fosfomisin dengan konsentrasi masing-masing 10%, 20% dan 30% (mg/mL). Pengujian biofilm dilakukan pada media Nutrient Broth (NB), menggunakan mikroplat U-bottom polystyrene 96 wells. Pewarnaan biofilm menggunakan kristal violet 1%. Optical Density (OD) biofilm dibaca pada λ 595 nm. Data hasil penelitian dianalisis dengan menggunakan Analysis Of Variance (ANOVA). Hasil penelitian menunjukkan aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm tergolong positif moderat, yaitu 2,667cell/µL. Uji Biofilm menunjukkan jenis dan konsentrasi antibiotik berpengaruh secara nyata (P<0,05) terhadap daya hambat biofilm Staphylococcus aureus isolat preputium sapi Aceh. Tetrasiklin 30% (mg/mL) mempunyai kemampuan paling baik menghambat pembentukan biofilm Staphylococcus aureus isolat preputium sapi Aceh sebesar 52,18% menjadi 1,262 cell/µL, sehingga aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm dari positif moderat menjadi positif palsu. Kata Kunci: biofilm, antibiotik, Staphylococcus aureus, sapi aceh.

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Inhibition Effect Extract Stolephorus insularis as a Antimicroba in Staphylococcus aereus Bacteria

DENTA ◽

10.30649/denta.v12i1.165 ◽

2018 ◽

Vol 12 (1) ◽

pp. 22

Author(s):

Ayu Listya ◽

Yulie Emilda Akhwan

Keyword(s):

Staphylococcus Aureus ◽

Root Canal ◽

Quantitative Research ◽

Media Culture ◽

Root Canal Treatment ◽

Research Needs ◽

Dmso Solution ◽

Entire Surface ◽

Inhibition Effect ◽

Inhibitory Power

<pre>Background : Untreated caries will continue and microorganisms enter the pulp resulting in an inflammatory response and pulp necrosis. Sterilization is one part of root canal treatment and is important for the success of root canal treatment. Stolephorus insularis contains antibacterial substances such as fluor. Previous research showed that extract Stolephorus insularis has antibacterial activity in Streptococcus mutans bacteria.</pre><pre>Purpose : This study aims to determine the antibacterial power of Stolephorus insularis extract on the growth of Staphylococcus aereus bacteria by using 3 concentrations of 18%, 24% and 30%.</pre><pre>Material and Method :This resesarch use culture of Staphylococcus aereus bacteria in MH blood agar, Stolephorus insularis extract with various concentrations (18%, 24%, 30%), 7% Na CMC, 1% DMSO solution, agar BHI media. Culture of Staphylococcus aereus was taken from liquid BHI media, then rubbed on the entire surface of BHI media using a sterile cotton stick. The antibacterial zone formed is measured.</pre><pre> </pre><pre>Result : Based on the results of the Mann-Whitney test there were significant differences in the inhibitory power of Staphylococcus aureus bacteria (p <0.05) in the K + group with groups K-, P1, P2 and P3. Whereas between groups P1 with K-, group P2 with K- and P1, and group P3 with K-, P1 and P2 there were no significant differences because the significant value was greater than 0.05</pre><pre>Conclusion : There is no antimicrobial power at concentrations of 18%, 24%, and 30%. Quantitative research needs to be done to determine the decrease in the number of bacterial colonies.</pre> Keywords : Inhibition effect, Stolephorus insularis, Staphylococcus aereus Correspondence : Ayulistya Paramita, Paediatric Departemen. FKG UHT, Jl Arief Rahman Hakim no 150 Surabaya. email : [email protected]

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The Antimicrobial and Anti-Inflammatory Effects of Silver Nanoparticles Synthesised from Cotyledon orbiculata Aqueous Extract

Nanomaterials ◽

10.3390/nano11051343 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1343

Author(s):

Caroline Tyavambiza ◽

Abdulrahman Mohammed Elbagory ◽

Abram Madimabe Madiehe ◽

Mervin Meyer ◽

Samantha Meyer

Keyword(s):

Staphylococcus Aureus ◽

Silver Nanoparticles ◽

Metallic Nanoparticles ◽

Methicillin Resistance ◽

Antimicrobial Activities ◽

Transmission Electron ◽

Anti Inflammatory ◽

Anti Inflammation ◽

Important Medicinal Plant ◽

Pro Inflammatory Cytokines

Cotyledon orbiculata, commonly known as pig’s ear, is an important medicinal plant of South Africa. It is used in traditional medicine to treat many ailments, including skin eruptions, abscesses, inflammation, boils and acne. Many plants have been used to synthesize metallic nanoparticles, particularly silver nanoparticles (AgNPs). However, the synthesis of AgNPs from C. orbiculata has never been reported before. The aim of this study was to synthesize AgNPs using C. orbiculata and evaluate their antimicrobial and immunomodulatory properties. AgNPs were synthesized and characterized using Ultraviolet-Visible Spectroscopy (UV-Vis), Dynamic Light Scattering (DLS) and High-Resolution Transmission Electron Microscopy (HR-TEM). The antimicrobial activities of the nanoparticles against skin pathogens (Staphylococcus aureus, Staphylococcus epidermidis, Methicillin Resistance Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans) as well as their effects on cytokine production in macrophages (differentiated from THP-1 cells) were evaluated. The AgNPs from C. orbiculata exhibited antimicrobial activity, with the highest activity observed against P. aeruginosa (5 µg/mL). The AgNPs also showed anti-inflammatory activity by inhibiting the secretion of pro-inflammatory cytokines (TNF-alpha, IL-6 and IL-1 beta) in lipopolysaccharide-treated macrophages. This concludes that the AgNPs produced from C. orbiculata possess antimicrobial and anti-inflammation properties.

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Synthesis and Evaluation of the Tetracyclic Ring-System of Isocryptolepine and Regioiso-Mers for Antimalarial, Antiproliferative and Antimicrobial Activities

Molecules ◽

10.3390/molecules26113268 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3268

Author(s):

Katja S. Håheim ◽

Emil Lindbäck ◽

Kah Ni Tan ◽

Marte Albrigtsen ◽

Ida T. Urdal Helgeland ◽

...

Keyword(s):

Prostate Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Antimicrobial Activities ◽

Ring System ◽

Human Prostate ◽

The Novel ◽

Human Prostate Cancer ◽

Biofilm Inhibition

A series of novel quinoline-based tetracyclic ring-systems were synthesized and evaluated in vitro for their antiplasmodial, antiproliferative and antimicrobial activities. The novel hydroiodide salts 10 and 21 showed the most promising antiplasmodial inhibition, with compound 10 displaying higher selectivity than the employed standards. The antiproliferative assay revealed novel pyridophenanthridine 4b to be significantly more active against human prostate cancer (IC50 = 24 nM) than Puromycin (IC50 = 270 nM) and Doxorubicin (IC50 = 830 nM), which are used for clinical treatment. Pyridocarbazoles 9 was also moderately effective against all the employed cancer cell lines and moreover showed excellent biofilm inhibition (9a: MBIC = 100 µM; 9b: MBIC = 100 µM).

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Antagonism between Aminoglycosides and β-Lactams in a Methicillin-Resistant Staphylococcus aureus Isolate Involves Induction of an Aminoglycoside-Modifying Enzyme

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.46.5.1516-1521.2002 ◽

2002 ◽

Vol 46 (5) ◽

pp. 1516-1521 ◽

Cited By ~ 7

Author(s):

Takashi Ida ◽

Ryoichi Okamoto ◽

Masato Nonoyama ◽

Kazuhiko Irinoda ◽

Mizuyo Kurazono ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Antimicrobial Activities ◽

Hybrid Structure ◽

Conjugative Plasmid ◽

Pcr Analysis ◽

Aminoglycoside Resistance ◽

Gene Encoding ◽

Methicillin Resistant ◽

Aureus Isolate

ABSTRACT We encountered three clinical isolates of methicillin-resistant Staphylococcus aureus which were susceptible to netilmicin and arbekacin in the absence of β-lactam antibiotics but which were resistant to them in the presence of β-lactam antibiotics. One of these strains, KU5801, was used to further investigate the antagonism between aminoglycosides and β-lactam antibiotics. β-Lactam antibiotics induced bacterial synthesis of aminoglycoside-6′-N-acetyltransferase and 2"-O-phosphotransferase [AAC(6′)-APH(2")] in association with decreased antimicrobial activities of aminoglycosides. A 14.4-kb EcoRI fragment that included the genes that control for β-lactam-inducible aminoglycoside resistance was cloned from a 31-kb conjugative plasmid present in KU5801. Restriction fragment mapping and PCR analysis suggested that a Tn4001-like element containing a gene encoding AAC(6′)-APH(2") was located downstream from a truncated blaZ gene. The DNA sequence between blaR1 and a Tn4001-like element was determined. The Tn4001-IS257 hybrid structure was cointegrated into the blaZ gene, and the typical sequences for the termination of transcription were not found between these regions. We deduced that antagonism of aminoglycosides by β-lactam antibiotics in isolate KU5801 involved transcription of the aac(6′)-Ie-aph(2")-Ia gene under the influence of the system regulating penicillinase production.

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Synthesis and antimicrobial activities of novel n-substituted 8-(1-alkyl/alkylsulphonyl/alkoxycarbonyl-benzimidazol-2-ylmethoxy)-5-chloroquinolines

Journal of the Serbian Chemical Society ◽

10.2298/jsc100817105c ◽

2011 ◽

Vol 76 (9) ◽

pp. 1199-1206 ◽

Cited By ~ 8

Author(s):

Raju Chaudhari ◽

Sahebrao Rindhe

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Nitrogen Heterocycles ◽

Antimicrobial Activities ◽

Mass Spectral Data ◽

Antifungal Activities ◽

Mass Spectral ◽

Electrophilic Reagents ◽

Almost All ◽

And Staphylococcus Aureus

Herein the synthesis of a series of novel 8-(1- alkyl/alkylsulphonyl/alkoxycarbonyl-benzimidazol-2-ylmethoxy)-5- chloroquinoline derivatives is reported. These derivatives were prepared by the condensation of o-phenylenediamine with [(5-chloroquinolin-8- yl)oxy]acetic acid, followed by substitution at nitrogen with different electrophilic reagents in presence of an appropriate base to give a series of nitrogen heterocycles containing the benzimidazole and quinoline nuclei. The structures of the compounds were confirmed based on 1H-NMR, 13CNMR, IR and mass spectral data. Almost all the compounds exhibited promising antibacterial activity against Salmonella typhimurium and Staphylococcus aureus. Some of the compounds showed good antifungal activities against Aspergillus niger but the antifungal activities against Candida albicans were disappointing.

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Highlighting the Biotechnological Potential of Deep Oceanic Crust Fungi through the Prism of Their Antimicrobial Activity

Marine Drugs ◽

10.3390/md19080411 ◽

2021 ◽

Vol 19 (8) ◽

pp. 411

Author(s):

Maxence Quemener ◽

Marie Dayras ◽

Nicolas Frotté ◽

Stella Debaets ◽

Christophe Le Meur ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Oceanic Crust ◽

Gene Clusters ◽

Multidrug Resistant ◽

Antimicrobial Activities ◽

Antimicrobial Compounds ◽

Human Pathogens ◽

Biotechnological Potential ◽

Fungal Isolates

Among the different tools to address the antibiotic resistance crisis, bioprospecting in complex uncharted habitats to detect novel microorganisms putatively producing original antimicrobial compounds can definitely increase the current therapeutic arsenal of antibiotics. Fungi from numerous habitats have been widely screened for their ability to express specific biosynthetic gene clusters (BGCs) involved in the synthesis of antimicrobial compounds. Here, a collection of unique 75 deep oceanic crust fungi was screened to evaluate their biotechnological potential through the prism of their antimicrobial activity using a polyphasic approach. After a first genetic screening to detect specific BGCs, a second step consisted of an antimicrobial screening that tested the most promising isolates against 11 microbial targets. Here, 12 fungal isolates showed at least one antibacterial and/or antifungal activity (static or lytic) against human pathogens. This analysis also revealed that Staphylococcus aureus ATCC 25923 and Enterococcus faecalis CIP A 186 were the most impacted, followed by Pseudomonas aeruginosa ATCC 27853. A specific focus on three fungal isolates allowed us to detect interesting activity of crude extracts against multidrug-resistant Staphylococcus aureus. Finally, complementary mass spectrometry (MS)-based molecular networking analyses were performed to putatively assign the fungal metabolites and raise hypotheses to link them to the observed antimicrobial activities.

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Antibacterial and anti-biofilm activities of crude extracts of Lawsonia inermis against Methicillin Resistant Staphylococcus aureus

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9i6.14362 ◽

2016 ◽

Vol 9 (6) ◽

pp. 263 ◽

Cited By ~ 1

Author(s):

Triveni A G ◽

Suresh Kumar Mendem ◽

Channapa T Shivannavar ◽

Subhaschandra M Gaddad

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Petroleum Ether ◽

Methicillin Resistant Staphylococcus Aureus ◽

Multi Drug Resistance ◽

Lawsonia Inermis ◽

Biofilm Inhibition ◽

Methicillin Resistant ◽

Crude Extracts

Objective:The continuous rise in the prevalence of multi drug resistance pathogens globally is threatening the treatment and management of infectious diseases. Ethno medicine plays a key role in the exploration for novel bioactive compounds. The present study evaluates the antibacterial and antibiofilm activities of the crude extracts of Lawsonia inermis against clinical isolates of methicillin resistant Staphylococcus aureus (MRSA).Materials and methods: Shade dried and finely powdered leaves of the plant were extracted by maceration method using six solvents methanol, acetone, ethyl acetate, chloroform, petroleum ether and n-hexane. Antibacterial and antibiofilm activities of the extracts against MDR MRSA by agar cup diffusion and tube method respectively.Results: Methanol extract showed the highest antibacterial activity of 18mm compared to other extracts. Similarly, petroleum ether extract showed highest biofilm inhibition of 84.7%. Other solvent extracts also exhibited significant biofilm inhibition [n-Hexane-83.6%, Ethyl acetate -79.5%, Chloroform-79.2%, Acetone -77% and Methanol-77%].Conclusion: The leaf extracts of L. inermis have shown promising biofilm inhibitory activity and good antibacterial activity, which can be explored for the development of new drugs for the MDR pathogens. Keywords: Antibioticacivity, Antibiofilm activity, L.inermis, MRSA

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