scholarly journals ĐẶC ĐIỂM BỆNH NHÂN NHIỄM KHUẨN TIẾT NIỆU PHỨC TẠP ĐIỀU TRỊ TẠI BỆNH VIỆN TRUNG ƯƠNG THÁI NGUYÊN

2022 ◽  
Vol 508 (2) ◽  
Author(s):  
Nguyễn Thị Nhung ◽  
Lưu Thị Bình
Keyword(s):  
Enterococcus Spp ◽  
Staphylococcus Spp

Mục tiêu: Mô tả đặc điểm lâm sàng, cận lâm sàng và căn nguyên vi khuẩn học của bệnh nhân nhiễm khuẩn tiết niệu (NKTN) phức tạp. Đối tượng và phương pháp nghiên cứu: Mô tả cắt ngang 100 bệnh nhân được chẩn đoán NKTN phức tạp tại Bệnh viện Trung ương Thái Nguyên (BVTWTN) từ tháng 02/2020 đến tháng 8/2021. Kết quả: Tỷ lệ nam/nữ = 0,79. Tuổi trung bình của các bệnh nhân là 61,64±17,15 với 60% bệnh nhân ≥60 tuổi. Thời gian nằm viện trung bình của các bệnh nhân là 14,22±7,49 ngày với 87% bệnh nhân nằm viện >7 ngày. 41% bệnh nhân có biểu hiện sốt, 33% với tiểu buốt và 49% với đau hông lưng. 41% bệnh nhân có suy thận, 40% với sỏi thận và 32% với bệnh lý suy giảm miễn dịch. 100% bệnh nhân có bạch cầu trong nước tiểu, 44% có hồng cầu niệu và 45% có nitrit niệu dương tính. Cấy nước tiểu: 91% dương tính với vi khuẩn Gram âm, trong đó vi khuẩn E.coli chiếm 61%, P.aeruginosa là 8%; E.coli nhạy cảm cao với fosfomycin (93,8%), meropenem (93,2%), piperacillin + tazobactam (84,9%) và amikacin (71,2%); kháng >50% các kháng sinh nhóm fluoroquinolone và các thế hệ của cephalosporin, kháng >80% nhóm betalactam và ức chế acid folic. Vi khuẩn Gram dương phân lập được hai chủng là Staphylococcus spp (4%) và Enterococcus spp (5%). Trong đó, Staphylococcus spp đã kháng với hầu hết các nhóm kháng sinh đang được sử dụng tại bệnh viện. Kết luận: NKTN phức tạp thường gặp ở những bệnh nhân cao tuổi, có bệnh lý thận mạn tính, sỏi tiết niệu hoặc tình trạng suy giảm miễn dịch. Triệu chứng lâm sàng thường gặp là sốt, rối loạn đi tiểu. Căn nguyên vi khuẩn gây NKTN phức tạp thường gặp là E.coli. Nhiều vi khuẩn có tỷ lệ đề kháng cao với các kháng sinh đang được sử dụng để điều trị NKTN tại bệnh viện.

scholarly journals Exposure Assessment of Airborne Bacteria Emitted from Swine Manure Composting Plant

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1283
Author(s):  
Ki-Youn Kim
Keyword(s):  
Relative Humidity ◽  
Environmental Factors ◽  
Swine Manure ◽  
Airborne Bacteria ◽  
Particulate Matters ◽  
Distribution Characteristics ◽  
E Coli ◽  
Enterococcus Spp ◽  
Staphylococcus Spp ◽  
Rotary Type

This study was performed to investigate the distribution characteristics of airborne bacteria emitted from swine manure composting plants. The types of swine manure composting plants selected for the survey in this study were as follows: screw type, rotary type, and natural dry type. Mean levels of airborne bacteria in swine manure composting plants were 7428 (±1024) CFU m−3 for the screw type, 3246 (±1407) CFU m−3 for the rotary type, and 5232 (±1217) CFU m−3 for the natural dry type, respectively. Based on the results obtained from this study, the swine manure composting plant operated by screw type showed the highest concentration of airborne bacteria, followed by the natural dry type and rotary type. The monthly concentration of airborne bacteria was the highest in August and the lowest in November, regardless of the type of swine manure composting plant. The respirable size of airborne bacteria accounted for about 50% of the total. The ratio of respirable to the total quantity of airborne bacteria was 50%. The correlation relationships between airborne bacteria and environmental factors (temperature, relative humidity, particulate matters, and odor) were not found to be significant in the swine manure composting plants. The predominant genera of airborne bacteria identified were Micrococcus spp., Staphylococcus spp., Escherichia(E-coli) spp., Enterococcus spp., and Enterobacteriaceae spp.

Bakterielle Harnwegsinfektionen bei Katzen

2016 ◽  
Vol 44 (04) ◽  
pp. 227-236 ◽  
Author(s):  
Clara von Vopelius-Feldt ◽  
Georg Wolf ◽  
Ralf S. Mueller ◽  
Reinhard K. Straubinger ◽  
Katrin Hartmann ◽  
...  
Keyword(s):  
Retrospektive Studie ◽  
Coli Isolate ◽  
E Coli ◽  
Klinische Relevanz ◽  
Enterococcus Spp ◽  
Staphylococcus Spp

ZusammenfassungZiel: Ermittlung der Prävalenz prädisponierender Begleiterkrankungen (BE) bei Katzen mit bakteriellen Harnwegsinfektionen (HWI) und der Prävalenz von Bakterienspezies bei verschiedenen BE sowie deren Sensibilität gegenüber den bei HWI häufig eingesetzten Antibiotika Doxycyclin, Trimethoprim-Sulfamethoxazol (TMS), Amoxicillin-Clavulansäure (AMC), Cephalothin und Enrofloxacin. Material und Methoden: In die retrospektive Studie wurden Katzen mit positiver Urinkultur im Zeitraum 2003–2009 eingeschlossen. Basierend auf den Daten der Krankenakten erfolgte eine Einteilung in vier Gruppen: Katzen mit systemischen prädisponierenden BE, Katzen mit lokalen prädisponierenden BE, Katzen mit Harnblasendauerkathetern (HBDK) und Katzen ohne dokumentierte BE. Zur Ermittlung der wahrscheinlichen Effektivität der Antibiotika wurden deren antimikrobielle Impact-Faktoren berechnet. Ergebnisse: In die Studie gingen 194 Katzen mit 219 bakteriellen Isolaten ein. Davon wiesen 78,4% (152/194) eine BE auf. 49,5% (96/194) hatten eine systemische BE und 28,9% (56/194) hatten eine lokale BE oder einen HBDK. Katzen mit systemischen BE waren signifikant älter als Katzen der anderen Gruppen und häufiger weiblich als Katzen mit lokaler BE und HBDK. Mehr als 50% der Katzen mit systemischer BE zeigten keine klinischen Symptome einer Erkrankung des unteren Harntrakts. Escherichia (E.) coli, gefolgt von Streptococcus spp., Staphylococcus spp. und Enterococcus spp. waren die am häufigsten vorkommenden Isolate. Dabei lag der Anteil der E.-coli-Isolate bei Katzen mit systemischer BE signifikant höher, während bei Katzen mit HBDK und Katzen mit anderen lokalen BE der Anteil an Streptococcus- und Staphylococcus-spp.-Isolaten signifikant höher war. Bei Katzen mit lokaler BE und Katzen mit HBDK ergaben sich niedrigere antimikrobielle Impact-Faktoren als bei Katzen der anderen Gruppen. Schlussfolgerung und klinische Relevanz: Ein Großteil der Katzen mit HWI leidet unter einer prädisponierenden BE. Katzen mit systemischer BE zeigen häufig keine Symptome einer Erkrankung des unteren Harntrakts. AMC und TMS waren in dieser Katzenpopulation die Antibiotika mit den höchsten antimikrobiellen Impact-Faktoren.

scholarly journals Identification of Bacteria Associated with Post-Operative Wounds of Patients with the Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Approach

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 5007
Author(s):  
Małgorzata Szultka-Młyńska ◽  
Daria Janiszewska ◽  
Paweł Pomastowski ◽  
Michał Złoch ◽  
Wojciech Kupczyk ◽  
...  
Keyword(s):  
Brain Heart Infusion ◽  
Culture Conditions ◽  
Clinical Samples ◽  
Bromocresol Purple ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Enterococcus Spp ◽  
Identification Of Bacteria ◽  
Staphylococcus Spp

The bacterial infection of post-operative wounds is a common health problem. Therefore, it is important to investigate fast and accurate methods of identifying bacteria in clinical samples. The aim of the study was to analyse the use of the MALDI-TOF MS technique to identify microorganism wounds that are difficult to heal. The most common bacteria are Escherichia coli, Staphylococcus spp., and Enterococcus spp. We also demonstrate the effect of culture conditions, such as the used growth medium (solid: Brain Heart Infusion Agar, Mueller Hilton Agar, Glucose Bromocresol Purple Agar, and Vancomycin Resistance Enterococci Agar Base and liquid: Tryptic Soy Broth and BACTEC Lytic/10 Anaerobic/F), the incubation time (4, 6, and 24h), and the method of the preparation of bacterial protein extracts (the standard method based on the Bruker guideline, the Sepsityper method) to identify factors and the quality of the obtained mass spectra. By comparing the protein profiles of bacteria from patients not treated with antibiotics to those treated with antibiotics based on the presence/absence of specific signals and using the UniProt platform, it was possible to predict the probable mechanism of the action of the antibiotic used and the mechanism of drug resistance.

scholarly journals Evaluation of sanitary quality of goat milk in dairy industries from the Cariri region, state of Paraíba

2021 ◽  
Vol 10 (12) ◽  
pp. e596101220735
Author(s):  
Iara Nunes de Siqueira ◽  
Aline Antas Cordeiro Cavalcanti ◽  
Joyce Galvão de Souza ◽  
Filipe Jordão Pereira de Medeiros ◽  
João Carlos Taveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Goat Milk ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Enterococcus Spp ◽  
Bacillus Spp ◽  
Thermotolerant Coliforms ◽  
Staphylococcus Spp

The sanitary evaluation of equipment and hands is fundamental to investigate the presence of pathogens in the dairy industry. Then, this study aims to evaluate the sanitization of equipment, workers’ hands, raw and pasteurized milk in goat milk dairies in the Cariri region, state of Paraíba.  Collected 32 samples of four dairies represented by letters A, B, C, and D. The followings contents were analyzed: mesophiles, total and thermotolerant coliforms, Escherichia coli, Staphylococcus aureus, Samonella spp. and Listeria monocytogenes in the reception tank, pasteurization tank, packing machine, package, wall, workers’ hand, and each dairy’s raw and pasteurized milk. After isolation, 84 colonies were confirmed by MALDI TOF. The indicator microorganisms presented variations for the workers’ hands, while A and B stayed within the patterns. For the equipment, only dairy B was within limits. They were out of the standard for mesophiles, total coliforms, and thermotolerant regarding raw and pasteurized milk. The microorganisms, the Enterobacteriaceae family presented a higher frequency, with 77.38%, and within this family, Escherichia coli, Klebsiella spp., and Enterobacter spp. were the most prevalent. Gram-positive corresponded to 22.62%, Bacillus spp., Staphylococcus spp., Enterococcus spp., and Macrococcus caseolyticus. Listeria monocytogenes and Salmonella spp. were not isolated. These demonstrate failures in goat milk processing with pathogenic bacteria in several dairy plants, indicating the need to adjust the product’s quality control.

scholarly journals Infecciones asociadas a procedimientos invasivos. Hospital de alta complejidad. Paraguay en eñ 2015

2017 ◽  
Vol 6 (1) ◽  
pp. 7
Author(s):  
Zunilda Garay
Keyword(s):  
Candida Tropicalis ◽  
Candida Sp ◽  
Enterococcus Spp ◽  
Factores De Riesgo ◽  
Acinetobacter Spp ◽  
Staphylococcus Spp

Las infecciones asociadas a la atención en salud (IAAS) prolongan la estadía hospitalaria, incrementan la mortalidad y los costos. El objetivo del estudio fue determinar las características de los pacientes, tasa de incidencia y los microorganismos aislados en las IAAS asociadas a dispositivos. Estudio, descriptivo, retrospectivo, realizado desde enero a diciembre del año 2015, que incluyó pacientes internados en la Unidad de Cuidados Intensivos de Adultos (UCIA), con catéter venoso central (CVC), ventilación mecánica invasiva (VM) y catéter urinario permanente (CUP). Los datos fueron extraídos a partir de la hoja de factores de riesgo y la ficha de notificación de IAAS utilizado para la vigilancia. Se analizaron 75 fichas de IAAS. La tasa de incidencia de neumonía asociada a ventilación mecánica (NAM) fue de 15,9/1.000 días de VM, la tasa de infección del torrente sanguíneo asociada a catéter venoso central (ITS/CVC) fue de 9,2/1.000 días CVC y la tasa de infección del tracto urinario asociado a catéter urinario permanente (ITU/CUP) fue de 4,5/1000 días de CUP. Los microorganismos más frecuentes fueron: en NAVM, A. baumannii complex 10/31 (32%), P. aeruginosa 8/31 (26%), K pneumoniae 5/31 (16%). En ITS/CVC S. aureus 7/28 (25%), K. pneumoniae 5/28 (18%), E. faecalis 5/28 (18%) y en ITU/CUP predominio de Candida sp 4/13 (31%), Candida tropicalis, 3/13 (23%), A. baumannii 2/13 (15%). El 80% de los Enterococcus spp resultaron resistentes a Vancomicina, 42% de los Staphylococcus spp fueron meticilino resistentes, el 70% de los Acinetobacter spp, el 33% de P. aeruginosa y fueron multiresistentes. Resultaron ser productores de Carbapenemasa el 33% de K. pneumoniae. Se ha observado alta resistencia para lo cual es fundamental la implementación de medidas preventivas. 

scholarly journals Monitoring and Characteristics of Major Mastitis Pathogens from Bulk Tank Milk in Korea

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1562 ◽  
Author(s):  
Mun-Jo Yun ◽  
Sunghyun Yoon ◽  
Young Ju Lee
Keyword(s):  
Somatic Cell Count ◽  
Milk Quality ◽  
Coagulase Negative Staphylococci ◽  
Bulk Tank Milk ◽  
E Coli ◽  
Enterococcus Spp ◽  
Resistance To Penicillin ◽  
Bulk Tank ◽  
Staphylococcus Spp ◽  
Mastitis Pathogens

In many countries, bulk tank milk (BTM) has been used for examining milk and analyzed as an important part of milk quality assurance programs. The objectives of this study were to investigate milk quality and the presence of major mastitis pathogens in BTM, and to compare the characteristics of BTM by dairy factory or company. A total of 1588 batches of BTM samples were collected from 396 dairy farms of seven dairy factories owned by four companies in Korea. The means of individual bacterial counts (IBC) and somatic cell count (SCC) were 3.7 × 104 cells/mL and 1.1 × 105 cells/mL, respectively, and no significant differences among dairy factories were observed. The most common pathogen was Staphylococcus spp. (60.1%), followed by E. faecalis (53.8%), E. coli (37.6%) and Streptococcus spp. (22.5%). Enterococcus spp. showed the highest resistance to tetracyclines (51.1% to 73.9%) and macrolides (46.5%). S. aureus and coagulase-negative staphylococci (CNS) showed the highest resistance to penicillin (28.4% and 40.2%, respectively), and three (3.2%) S. aureus and seven (3.3%) CNS were also methicillin-resistant. These data show the diverse prevalence and characteristics of major mastitis pathogens among factories, and support the development of strong monitoring and prevention programs of mastitis pathogens by commercial dairy operations.

scholarly journals Performance of Vitek 2 for Antimicrobial Susceptibility Testing of Staphylococcus spp. and Enterococcus spp.

2013 ◽  
Vol 52 (2) ◽  
pp. 392-397 ◽  
Author(s):  
A. M. Bobenchik ◽  
J. A. Hindler ◽  
C. L. Giltner ◽  
S. Saeki ◽  
R. M. Humphries ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Enterococcus Spp ◽  
Vitek 2 ◽  
Staphylococcus Spp

scholarly journals Análisis epidemiológico de las infecciones urinarias en pacientes ambulatorios del Centro Médico Bautista

2020 ◽  
Vol 8 ◽  
pp. 109
Author(s):  
Lisa Ibarrola de Ávila ◽  
Lourdes Talavera ◽  
María Marta González ◽  
Fernando Ávila
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Spp ◽  
Enterococcus Spp ◽  
Las Mujeres ◽  
Staphylococcus Spp ◽  
Los Grupos ◽  
Klebsiella Spp

Las infecciones del tracto urinario son las infecciones más comunes en todo el mundo y se presentan en todos los grupos de población, se estima que entre el 40% y el 50% de las mujeres  presentarán al menos una durante su vida, y de estas el  11% tendrá una infección al año, siendo la causa más frecuente de origen bacteriano (80%-90%). Constituyen una causa frecuente de consulta en atención primaria, afecta mayoritariamente a las mujeres, generalmente son monobacterianas ,sus  agentes etiológicos incluyen :  Escherichia coli, Klebsiella spp, Proteus spp, Enterococcus spp, Pseudomonas spp y Staphylococcus spp, los cuales presentan prevalencias y perfiles de susceptibilidad antibiótica diferentes entre poblaciones por lo que es relevante contar con esta  información para generar un perfil clínico-epidemiológico propio que permita la implementación de medidas terapéuticas correctas, así como  la atención y control de la resistencia bacteriana, acordes con las particularidades de la población del lugar de estudio.

scholarly journals A Preliminary Study on Antimicrobial Susceptibility of Staphylococcus spp. and Enterococcus spp. Grown on Mannitol Salt Agar in European Wild Boar (Sus scrofa) Hunted in Campania Region—Italy

Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 85
Author(s):  
Francesca Paola Nocera ◽  
Gianmarco Ferrara ◽  
Emanuela Scandura ◽  
Monica Ambrosio ◽  
Filomena Fiorito ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Wild Boar ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Mannitol Salt Agar ◽  
Campania Region ◽  
Wild Boars ◽  
Nasal Cavities ◽  
Enterococcus Spp ◽  
Staphylococcus Spp

The importance of wild boar lies in its role as a bioindicator for the control of numerous zoonotic and non-zoonotic diseases, including antibiotic resistance. Mannitol Salt Agar (MSA) is a selective medium used for isolation, enumeration, and differentiation of pathogenic staphylococci. Other genera such as Enterococcus spp. are also salt tolerant and able to grow on MSA. The present study focused on the identification, by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS), of bacteria grown on MSA isolated from the nasal cavities of 50 healthy wild boars hunted in Campania Region (southern Italy) in the year 2019. In addition, the antimicrobial resistance phenotype of the isolated strains was determined by disk diffusion method. Among genus Staphylococcus, coagulase-negative Staphylococcus (CoNS) were the most common isolated species, with Staphylococcus xylosus as the most prevalent species (33.3%). Furthermore, Enterococcus spp. strains were isolated, and Enterococcus faecalis was the species showing the highest frequency of isolation (93.8%). For staphylococci, high levels of resistance to oxacillin (93.3%) were recorded. Differently, they exhibited low frequencies of resistance to tested non-β-lactams antibiotics. Among enterococci, the highest resistances were observed for penicillin (93.7%), followed by ampicillin (75%), and ciprofloxacin (68.7%). Interestingly, 43.7% of the isolated strains were vancomycin-resistant. In conclusion, this study reports the phenotypic antibiotic resistance profiles of Staphylococcus spp. and Enterococcus spp. strains isolated from nasal cavities of wild boars hunted in Campania Region, highlighting that these wild animals are carriers of antibiotic resistant bacteria.

scholarly journals Value of polymerase chain reaction in etiological diagnostic of infectiove endocarditis

2021 ◽  
Vol 42 (Supplement_1) ◽  
Author(s):  
E O Kotova ◽  
E A Domonova ◽  
A S Pisaryuk ◽  
O Y Silveystrova ◽  
Y L Karaulova ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Microbiological Method ◽  
Chain Reaction ◽  
E Coli ◽  
Enterococcus Spp ◽  
Acinetobacter Spp ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Staphylococcus Spp ◽  
Culture Negative

Abstract Introduction Identification of a causative agent in patients with infective endocarditis (IE) is crucial for diagnostic and prescribing etiotropic therapy which defines positive outcome of a disease. High rate of a culture negative IE and inaccurate results of traditional microbiological methods raise a concern. So the methods of etiological diagnostics in IE are in need of development, particularly introduction of polymerase chain reaction (PCR) method might be helpful. Aim Modernisation of the algorithm of IE etiological diagnostic by introducing PCR Materials and methods The study included 85 cases of IE [first episode of IE (n=79), recurrence/relapse (n=6)] verified by DUKE criteria 2009, 2015, hospitalized in Moscow primary hospital from 2012 to 2017. All patient had venous blood investigated both with microbiological method and with broadrange and specific PCR. Following microorganisms' DNA were assessed by PCR: Staphylococcus spp. (MRCoNS, S. aureus and others), Streptococcus spp. (S. agalactiae, S. pyogenes and others), Enterococcus spp. (E. faecium, E. faecalis and others), Enterobacteriaceae, Klebsiella spp. (K. pneumoniae and others), E. coli, Proteus spp., A. baumanii, P. aeruginosa, Fungi (C. albicans, C. glabrata, Aspergillus spp. and others). Results Median age was 55.48 years (95% confidence interval (CI) 51.16–59.8), males 67.9%. History of cardiovascular diseases was in 54 (68.35%), diabetes mellitus in 18 (22.78%), hepatitis B and/or C in 31 (39.2%), intravenous drug dependency in 27 (34.18%), chronic kidney disease in 38 (48.1%), median Charlson comorbidity index was 5.44 (95% CI 4.52–6.37). Left-side IE was in 50 (63.29%), right-side IE – in 23 (29.12%), left-right-side IE in 6 (7.59%). Secondary IE was in 53 patients (62.3%). Embolic events were diagnosed in 27 cases (34.18%), in-hospital mortality – in 22 (27.8%). Microbiological method identified etiological agent in 55 of 85 cases (61.2%), featuring Staphylococcus aureus (n=23), Staphylococcus CoNS (n=6), Escherichia coli (n=1), Acinetobacter spp. (n=2), Streptococcus spp. (n=2), Enterococcus spp. (n=8), Klebsiella pneumoniae (n=2), Gemella haemolysans (n=2), several causative agents (n=6). Additional PCR testing identified etiology in 14 of 33 (42.2%) featuring Staphylococcus spp. (n=6), Enterococcus spp. (n=3), Streptococcus spp. (n=1), Aspergillus sp. (n=1) Pasteurella multocida (n=1), Enterococcus spp. + Staphylococcus spp. (n=1), Staphylococcus spp.+ A. baumanii + E. coli (n=1). PCR method identified 6 fals-positive results of microbilogical investigation [S. epidermidis (n=2), G. haemolysans, Acinetobacter spp., E. faecalis, K. pneumoniae], that are most probably due to preanalytical sample contamination Conclusions Introduction of PCR into the algorithm of IE etiological diagnostic increased validity of laboratory findings on 23.5%. True culture negative IE was present in 19 of 85 patients. Rate of mortality and complications in IE remains high. FUNDunding Acknowledgement Type of funding sources: Public Institution(s). Main funding source(s): Peoples' Friendship University of Russia (RUDN), Moscow, Russia

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