scholarly journals EVALUATION OF HEPATOPROTECTIVE POTENTIAL OF LEAF AND LEAF CALLUS EXTRACTS OF ANISOCHILUS CARNOSUS (L) WALL.

2018 ◽  
Vol 10 (3) ◽  
pp. 156
Author(s):  
Nissar Ahmad Reshi ◽  
Sudarahana Mysore Shankarsingh ◽  
Girish Hodiyala Vasanaika
Keyword(s):  
Secondary Metabolites ◽  
Cell Viability ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Alcohol Intoxication ◽  
Leaf Explants ◽  
Hepatoprotective Activity ◽  
Phytochemical Analysis ◽  
Toxic Dose ◽  
Viability Percentage

<p>The study was carried out to evaluate the hepatoprotective activity of leaf and leaf callus extracts of <em>Anisochilus carnosus</em> (L) Wall. against alcohol induced toxicity using HepG2 cell line. Leaf explants were cultured on Murashige and Skoog solid medium supplemented with different growth regulators. Prior to the determination of hepatoprotective property leaf and leaf callus extracts were subjected to the toxic dose study. The degree of hepatoprotection of extracts was determined by measuring cell viability percentage by MTT assay. The preliminary phytochemical analysis of leaf and leaf callus was carried out by qualitative analysis. Maximum percentage of callus formation (98%) was obtained in MS medium fortified with 3 mg/l 2,4-D. HepG2 cells were pretreated with the different concentrations (below toxic dose) of leaf and leaf callus extracts for 72 hours followed by alcohol intoxication. Results revealed that ethanolic leaf extract pretreated HepG2 cells show 94% cell viability compared to the standard silymarin pretreated HepG2 cells which showed 81% cell viability. Leaf callus extracts also exhibited significant hepatoprotective activity where ethanolic callus extract pretreated HepG2 cells showed 86% viability after intoxication with alcohol. Results revealed that HepG2 cell viability percentage is dose dependent. Phytochemical studies revealed the presence of different secondary metabolites in leaf and leaf callus extracts. The bio-efficacy study confirms the presence of secondary metabolites of hepatoprotective nature in leaf and leaf callus of <em>A. carnosus.</em></p>

scholarly journals Hepatoprotective activity of Flacourtia jangomas (Lour.) Raeuschleaves and fruit methanolic extract on paracetamol-induced hepatotoxicity in HepG2 Cells

Biomedicine ◽  
2021 ◽  
Vol 41 (3) ◽  
pp. 587-591
Author(s):  
Akshaya Pai ◽  
Chandrakala Shenoy
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Negative Control ◽  
Hepg2 Cell Line ◽  
Fruit Extract ◽  
Deciduous Fruit

Introduction and Aim: Plants have become the current focus of research in treating the various diseases and ailments. Flacourtia jangomas (Lour.) Raeusch belongs to the familySalicaceae. Itis a small deciduous fruit tree having immense nutritional and medicinal significance. Different parts of the plant are pharmaceutically used forcuring various ailments. In this study, we investigated the hepatoprotective activity of Flacourtia jangomas (Lour.) Raeusch leaves and fruit methanolic extract on Paracetamol induced HepG2 cell line.   Methods: The cytotoxic and hepatoprotective properties were evaluated by measuring cell viability; activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH); lipid peroxidation (malondialdehyde (MDA) levels).   Results:The increased cell viability of 140.43± 4.07% and 133.93±3.20%was observed in HepG2 cells treated with methanolic extract of F. jangomas leaf and fruit extract respectively at 10µg/ml concentration and then decreased along with the rise of F. jangomas leaf and fruit extract concentrations. The level of LDH, ALT, AST and MDA decreased after F. jangomas leaf and fruit treatment compared to negative control.   Conclusion: This study suggests that the methanolic Extract of F. jangomas (Lour.) Raeusch leaves(FJL)and fruit (FJF) shows hepatoprotective activity in Paracetamol induced HepG2 cell line by the decrease in AST and ALT activities and LDH and MDA level. Hence, it could be considered as a therapeutic agent in curing liver-related diseases.  

scholarly journals The hepatoprotective effects of Pyrus biossieriana buhse leaf extract on tert-butyl hydroperoxide toxicity in HepG2 cell line

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Hamed Mir ◽  
Daniel Elieh Ali Komi ◽  
Mahdi Pouramir ◽  
Hadi Parsian ◽  
Ali Akbar Moghadamnia ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Leaf Extract ◽  
Dry Weight ◽  
P Value ◽  
Hepg2 Cell Line ◽  
Butyl Hydroperoxide ◽  
Cell Growth Suppression

Abstract Objective In present study, the effects of the leaf extract of Pyrus biossieriana Buhse on tert-Butyl hydroperoxide (t-BHP) induced toxicity in the HepG2 cell line were investigated. Results HepG2 cells were exposed to different concentrations of both extract (1.5, 2.0, and 2.5 mg/mL) and t-BHP (100, 150, and 200 μM). The total flavonoid and phenolic contents, the cell viability, lipid peroxidation, NO generation, and the total antioxidant capacity in cell media were assessed. The amount of arbutin was estimated 12.6% of the dry weight of leaves (equivalent to 126 mg/g). Additionally, the amounts of flavonoids and phenols in extract were estimated 119 mg/g and 418 mg/g, respectively. The cells incubated with t-BHP showed a significant decrease in survival (p < 0.001). Preincubation with extract (1.5 mg/mL and 2.0 mg/mL) attenuated the t-BHP toxicity and increased the cell viability in cells exposed even to the highest concentration of t-BHP (200 μM) (p value < 0.001, and p value = 0.035) respectively. Additionally, treatment with extract reduced the cell growth suppression caused by t-BHP. The P. biossieriana Buhse leaf extract at concentrations of 1.5 and 2.0 mg/mL is capable of attenuating t-BHP-induced cytotoxicity in HepG2 cells.

scholarly journals Curcumin Protects HepG2 Cells from the Cytotoxic Effect of Drugs with Anti-fibrotic Activity

2021 ◽  
Author(s):  
Mariana Y. Medina-Pizaño ◽  
Marina N. Medina-Rosales ◽  
Esperanza Sánchez-Alemán ◽  
Sandra L. Martínez-Hernández ◽  
Liseth R. Aldaba-Muruato ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Morphological Changes ◽  
Liver Parenchyma ◽  
Hepg2 Cell Line ◽  
Secondary Effects ◽  
Hamster Model ◽  
Hematoxylin And Eosin

Abstract Background: The α and β adrenoblockers have been tested as an alternative treatment for chronic liver lesions such as fibrosis and cirrhosis in animal models, as well as their possible participation during the regeneration of the damage caused by liver cirrhosis in a hamster model. However, it was observed that doxazosin caused slight morphological changes in hepatocytes, while that curcumin showed protection to the hepatic parenchyma. Regardless, the pharmacokinetic effects of these 𝛼/𝛽 adrenoblockers on the hepatocytes' cell viability, possibly involved in the hepatic parenchyma's repopulation during cirrhosis reversal, are unknown. The present study aimed to elucidate the protective effect of curcumin on the possible side effects of doxazosin, tamsulosin, and carvedilol on the HepG2 cell line, drugs already tested with antifibrotic activity.Methods: HepG2 cells were exposed to 0.1, 0.5, 10, and 25 µM of doxazosin, carvedilol, and tamsulosin for 24, 48, and 72 h, for curcumin, cells were pretreated with 1 µM for 1 h before exposure to α and β adrenoblockers. The cell viability was assessed by MTT assay. The morphological changes were determined using hematoxylin and eosin (H&E) staining, scanning electron microscope (SEM), and acridine orange (AO) staining. Results: We observed that the doxazosin decreases cell viability dependently time and dose; carvedilol and tamsulosin increase cell proliferation. However, curcumin induces regulation of these effects in HepG2 cell line, increasing or maintaining viability compared to control. The pretreatment with curcumin regulated AST levels (aspartate aminotransferase) and ALT (alanine aminotransferase) in cells exposed to α and β adrenoblockers. The SEM and H&E staining provided evidence that doxazosin, carvedilol, and tamsulosin induced morphological changes in HepG2 cell line, depending on time and dose, approximately 80% of the cells treated with drugs were balonized, and curcumin protected these effects, maintaining the morphology in 90% of the treated cells.Conclusions: The present study demonstrates that curcumin protected the HepG2 cells against cytotoxicity and morphological changes induced by the α and β adrenoblockers attenuating secondary effects for possible oxidative stress. In this way, it is concluded that these treatments with antifibrotic effect, in co-treatment with curcumin, will not affect the possible repopulation process of the liver parenchyma during the reversion of fibrosis.

scholarly journals PHYTOCHEMICAL ANALYSIS OF CALLUS TWO VARIETIES ORTHOSIPHON ARISTATUS (BLUME) MIQ ON MURASHIGE AND SKOOG MEDIA: A STRATEGIC STEP OF SECONDARY METABOLITE PRODUCTION

2021 ◽  
pp. 71-77
Author(s):  
FAHRAUK FARAMAYUDA ◽  
TOTIK SRI MARIANI ◽  
ELFAHMI ◽  
SUKRASNO
Keyword(s):  
Secondary Metabolites ◽  
Rosmarinic Acid ◽  
High Performance ◽  
Leaf Explants ◽  
Gradient Elution ◽  
Growth Media ◽  
Phytochemical Analysis ◽  
New Information ◽  
Sterile Leaf

Objective: The research aimed to provide new information regarding the secondary metabolites content of purple and white-purple Orthosiphon aristatus (Blume) Miq. callus, which can then be used as a basis for developing towards cell suspension and ultimately producing secondary metabolites using bioreactors. Methods: Callus induction of two varieties of O. aristatus were performed by inoculating sterile leaf explants grown on Murashige and Skoog basal media supplemented with 2,4-dichlorophenoxyacetis acid 0.4 ppm. The secondary metabolites were analysed and quantified using high-performance liquid chromatography with gradient elution. Results: The results showed the growth of callus two varieties of O. aristatus in growth media MS with 2,4-D 0.4 ppm. Rosmarinic acid content in the acetone extract of the purple variety callus was 1.28% w/w, and the white-purple variety was 2.22% w/w. Conclusion: This study could form the basis for the development of rosmarinic acid production by In vitro culture modification.

scholarly journals Assessment of anticancer activity of Pulicaria undulata on hepatocellular carcinoma HepG2 cell line

Tumor Biology ◽  
2019 ◽  
Vol 41 (10) ◽  
pp. 101042831988008 ◽  
Author(s):  
Manal A Emam ◽  
Hemmat I Khattab ◽  
Marwa GA Hegazy
Keyword(s):  
Hepatocellular Carcinoma ◽  
Antitumor Activity ◽  
B Cell ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Antitumor Drugs ◽  
Phytochemical Analysis ◽  
Hepg2 Cell Line

Searching for new sources of safe nutraceuticals antitumor drugs is an important issue. Consequentially, this study designed to assess the antitumor activity of Pulicaria undulata extract in vitro in the treatment of hepatocellular carcinoma HepG2 cell line. Aerial parts of P. undulata plants were collected, used for phytochemical analysis, and assessed for anticancer activity. The antitumor activity was evaluated through studying the cell viability and apoptotic pathway. The gas chromatography–mass spectrometry phytochemical analysis revealed that P. undulata is a promising new source of several known antioxidant and antitumor compounds which could participate in drug development and exploration of alternative strategies to the harmful synthetic antitumor drugs. P. undulata stifled HepG2 cell viability in a concentration-dependent manner. Meanwhile, P. undulata tempted substantial apoptosis in HepG2 cells and enhanced the expression of miR-34a. However, the mRNA expression level of antiapoptotic B-cell lymphoma-2 was markedly decreased by P. undulata treatment. Moreover, P. undulata increased the protein expression of proapoptotic p53 and caspase 3/9 with reducing B-cell lymphoma-2 protein expression level. Thus, P. undulata induced apoptosis in the HepG2 cells by overexpression of miR-34a which regulates p53/B-cell lymphoma-2/caspases signaling pathway. These findings were well appreciated with morphological studies of cells treated with P. undulata. In conclusion, P. undulata could be a probable candidate agent for the initiation of cell apoptosis in HepG2 and thereby can serve as promising therapeutic agent for treatment of hepatocellular carcinoma which should attract further studies.

scholarly journals Hepatoprotective Activity of Bergenin against Xenobiotics-Induced Oxidative Stress in Human Hepatoma (HepG2) Cells

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Yollada Sriset ◽  
◽  
Waranya Chatuphonprasert ◽  
Kanokwan Jarukamjorn ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Viability ◽  
Gallic Acid ◽  
Cell Morphology ◽  
Hepg2 Cells ◽  
Hepatoprotective Activity ◽  
Potential Candidate ◽  
Human Hepatoma ◽  
Human Hepatoma Hepg2 Cells ◽  
Hepatoma Hepg2

Bergenin, a natural derivative of gallic acid, has been shown to exert anti-oxidant and anti-inflammatory activities. This study aimed to determine hepatoprotective activity of bergenin against ethanol and tert-butyl hydroperoxide (TBHP) induced oxidative stress in human hepatoma (HepG2) cells. HepG2 cells (5x105 cells/well) were co-treated with ethanol (100 mM) or TBHP (100 µM) and either bergenin (75, 150, and 300 µM) or gallic acid (60 µM, positive control) for 24 h. Cell viability, hematoxylin and eosin staining of cell morphology, cellular injury biomarkers: lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and malondialdehyde (MDA), and antioxidant biomarkers: superoxide dismutase (SOD), catalase (CAT), and total glutathione (GSH) content, were determined. Ethanol and TBHP decreased cell viability (86.67% and 84.49% of control, respectively), increased LDH toxicity (12.53% and 15.91% of control, respectively), and increased AST, ALT, and MDA levels, compared with the control. Based on cell morphology, both ethanol and TBHP injured HepG2 cells causing the loss of cell nuclei. Treatment of HepG2 cells with either ethanol or TBHP reduced SOD and CAT activities and depleted total GSH content, compared with the control. Bergenin and gallic acid improved the cell morphology, elevated cell viability (95.94-99.20% and 97.72-99.62% of control, respectively), lowered LDH toxicity (8.14-9.10% and 7.82-8.92% of control, respectively), restored AST, ALT, and MDA levels, promoted SOD and CAT activities, and enhanced the total GSH content of ethanol- and TBHP-treated HepG2 cells. Bergenin exhibited hepatoprotective activity via restoration of the oxidant-antioxidant system and is a potential candidate for hepatoprotective treatment.

scholarly journals Effects of Lidocaine-Mediated CPEB3 Upregulation in Human Hepatocellular Carcinoma Cell Proliferation In Vitro

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Hongjun Liu ◽  
Yiru Wang ◽  
Bing Chen ◽  
Xia Shen ◽  
Wenxian Li
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Proliferation ◽  
Antitumor Activity ◽  
Cell Viability ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Human Hepatocellular Carcinoma ◽  
Dependent Manner ◽  
Real Time Quantitative Pcr

Lidocaine displays antitumor activity by inducing apoptosis and suppressing tumor growth in human hepatocellular carcinoma (HepG2) cells in vitro. However, the molecular mechanism underlying lidocaine-mediated antitumor activity is unclear. In this study, HepG2 cells were treated with lidocaine, and cell proliferation and colony-forming ability were assessed. The expression level of cytoplasmic polyadenylation element binding protein 3 (CPEB3) was detected by real-time quantitative PCR and western blot. Lidocaine treatment resulted in decreased HepG2 cell viability and colony formation in a dose-dependent manner. In hepatocellular carcinoma patient samples, CPEB3 was downregulated and was associated with poor prognosis and high-grade malignancy. Additionally, CPEB3 was a critical mediator of lidocaine-induced repression of HepG2 cell proliferation. These results demonstrated that lidocaine decreased cell viability and colony-forming ability of HepG2 cells by upregulating CPEB3 expression.

Hydrogen peroxide and quercetin induced changes on cell viability, apoptosis and oxidative stress in HepG2 cells

2020 ◽  
Vol 01 ◽  
Author(s):  
Ayşe Mine Yılmaz ◽  
Gökhan Biçim ◽  
Kübra Toprak ◽  
Betül Karademir Yılmaz ◽  
Irina Milisav ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Cycle ◽  
Hydrogen Peroxide ◽  
Cell Viability ◽  
Hepg2 Cells ◽  
Proteasome Activity ◽  
Cell Cycle Phases ◽  
Induced Changes ◽  
And Oxidative Stress ◽  
Quercetin Treatment

Background: Different cellular responses influence the progress of cancer. In this study, we have investigated the effect of hydrogen peroxide and quercetin induced changes on cell viability, apoptosis and oxidative stress in human hepatocellular carcinoma (HepG2) cells. Methods: The effects of hydrogen peroxide and quercetin on cell viability, cell cycle phases and oxidative stress related cellular changes were investigated. Cell viability was assessed by WST-1 assay. Apoptosis rate, cell cycle phase changes and oxidative stress were measured by flow cytometry. Protein expressions of p21, p27, p53, NF-Kβ-p50 and proteasome activity were determined by Western blot and fluorometry, respectively. Results: Hydrogen peroxide and quercetin treatment resulted in decreased cell viability and increased apoptosis in HepG2 cells. Proteasome activity was increased by hydrogen peroxide but decreased by quercetin treatment. Conclusion: Both agents resulted in decreased p53 protein expression and increased cell death by different mechanisms regarding proteostasis and cell cycle phases.

Phytochemical Analysis of Polyphenol Secondary Metabolites in Cloudberry (Rubus Chamaemorus L.) Leaves

2021 ◽  
Author(s):  
A. K. Whaley ◽  
A. O. Ponkratova ◽  
A. A. Orlova ◽  
E. B. Serebryakov ◽  
S. N. Smirnov ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Phytochemical Analysis ◽  
Rubus Chamaemorus

scholarly journals Antihepatotoxic Activity of Phyllanthus atropurpureus Cultivated in Egypt

2011 ◽  
Vol 66 (9-10) ◽  
pp. 447-452 ◽  
Author(s):  
Taha Sarg ◽  
Afaf Abdel Ghani ◽  
Rawia Zayed ◽  
May El-Sayed
Keyword(s):  
Liver Injury ◽  
Active Ingredient ◽  
Ornamental Plants ◽  
Hepatoprotective Activity ◽  
Maximum Activity ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Aerial Parts ◽  
Serum Aspartate Aminotransferase ◽  
Antihepatotoxic Activity

The genus Phyllanthus (family Euphorbiaceae) is considered one of the important medicinal and ornamental plants. A phytochemical analysis of the extracts was performed to search for the active ingredient. Results of the investigation of the hepatoprotective activity of Phyllanthus atropurpureus Boj. Hort. Maurit. revealed that the activities of alcoholic extracts of its aerial parts and roots were quite similar to those of silymarin. Both of them improve the parameters of CCl4-induced liver injury including serum aspartate aminotransferase and alanine aminotransferase. Among the extracts tested, the root extract showed maximum activity compared to the aerial parts extract and to silymarin.

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