scholarly journals PHYTOCHEMICAL ANALYSIS OF THE LEAF EXTRACTS OF NYCTANTHES ARBOR-TRISTIS

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Rajani KS ◽  
Swasha Khandeparker Naik ◽  
Subhadarshini Satapathy ◽  
Sweta Mishra
Keyword(s):  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Bioactive Molecules ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Primary And Secondary Metabolites ◽  
Percolation Method ◽  
Indian Origin ◽  
Wide Range

The present study highlights preliminary phytochemical analysis of the leaf extract of Nyctanthes arbor-tristis. Plants produce a wide range of bioactive molecules which make them as a rich source of various types of medicines. Nyctanthes arbor-tristis is one of the well-known medicinal herbs of Indian origin belonging to family Oleaceae, commonly known as night Jasmine. The leaf extract is taken by percolation method. Various bioactive primary and secondary metabolites were tested using colour reactions with specific reagents. The study reveals that it contains some bioactive compounds which indicate that this plant might be used as anti-malarial, anti-viral and antidiabetic.

scholarly journals PRELIMINARY SCREENING OF PHYTOCHEMICALS AND ANTIPROLIFERATIVE AND ANTI-INFLAMMATORY PROPERTIES OF THESPESIA POPULNEA (L.) SOLAND LEAF EXTRACTS

2018 ◽  
Vol 11 (5) ◽  
pp. 382
Author(s):  
Megha Kb ◽  
Sanith Cheriyamundath ◽  
Joseph Madassery ◽  
Elyas Kk
Keyword(s):  
Leaf Extract ◽  
Chemical Constituents ◽  
Biologically Active ◽  
Bioactive Molecules ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Thespesia Populnea ◽  
Wide Range ◽  
Anti Inflammatory

Objective: Plant-derived bioactive molecules are providing infinite opportunities for new drug development as they possess a wide range of actions against diseases with lesser side effects. The present study is made to analyze the qualitative phytochemicals and to evaluate in vitro antiproliferative and anti-inflammatory potentials of leaf extracts of Thespesia populnea. Methods: T. populnea leaf extracts were prepared sequentially according to the increasing polarity of the solvents, i.e., petroleum ether, chloroform, ethyl acetate, and methanol. Qualitative phytochemical analysis was performed to identify the chemical constituents of the extracts, and antiproliferative properties were evaluated against different cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide assay. Bovine serum albumin anti-denaturation assay was done to identify the anti-inflammatory activity.Results: Phytochemical analysis of the extracts revealed the presence of alkaloids, terpenoids, glycosides, tannins, flavonoids, and phenolics. The chloroform extract (CHFE) of T. populnea has a dose-dependent antiproliferative effect against acute T-cell leukemia (Jurkat E6-1), prostate Grade IV adenocarcinoma (PC-3), mouse fibroblast (L-929), and monkey kidney normal (Vero) cells. Their inhibitory concentration 50% (IC50) values were found to be 35.73±0.94 μg/ml for Jurkat E6-1, 60.79±1.84 μg/ml for PC-3, 60.88±1.45 μg/ml for L-929, and 83.482±2.05 μg/ml for Vero, respectively. CHFE also displayed the anti-inflammatory potential.Conclusion: The chloroform leaf extract of T. populnea possesses potent antiproliferative and anti-inflammatory activity. These properties present in leaf extract may be explained by the presence of biologically active constituents.

Phytochemical evaluation of leaf extracts of Naringi crenulata (roxb.) Nicolson

2016 ◽  
Vol 5 (11) ◽  
pp. 5110
Author(s):  
Sartaj Ahmad Allayie ◽  
Mushtaq Ahmed Parray* ◽  
Bilal Ahmad Bhat ◽  
S. Hemalatha
Keyword(s):  
Quantitative Analysis ◽  
Solvent System ◽  
Great Promise ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Traditional Medicines ◽  
The People ◽  
Wide Range ◽  
Rf Values

The use of traditional medicines holds a great promise as an easily available source as effective medicinal agents to cure a wide range of ailments among the people particularly in tropical developing countries like India. The present study investigates the qualitative and quantitative analysis of the major bioactive constituents of N. crenulata leaf extracts. The extractive values of aqueous, acetone and chloroform extracts were found to be 11.34, 4.24 and 6.06 respectively. Qualitative phytochemical analysis of these three solvent extracts confirm the presence of Alkaloids, Saponins, Flavonoids and Phenolic compounds in all the three extracts; however, these phytochemicals were more significant in aqueous extract. Quantitative analysis was carried out using TLC method by different solvent system. Amongst various solvent systems, Butanol: acetic acid: water (9: 0.9: 0.1 v/v/v) shows maximum resolution and number of spots produced at long UV (365 nm) and under iodine vapours. The TLC chromatograms constituted different coloured phytochemical compounds with different Rf values. It can be conveniently used to evaluate the quality of different area samples. This indicates that the leaves can be useful for treating different diseases because the therapeutic activity of a plant is due to the presence of particular class of compounds and thus can serve as potential sources of useful drugs in future.

scholarly journals QUANTITATIVE ANALYSIS OF BIOACTIVE COMPOUNDS OF ARTEMISIA NILAGIRICA (CLARKE) PAMP. LEAF EXTRACT

2016 ◽  
pp. 183
Author(s):  
Parameswari P ◽  
Devika Rengaswamy
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Quantitative Analysis ◽  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Metabolic Reaction ◽  
Quantitative Investigation ◽  
Artemisia Nilagirica ◽  
Quantitative Examination

<p>ABSTRACT<br />Objective: The points of this exploration work were to decide the quantitative examination of bioactive mixes. Customarily, cutting edge meds rely<br />on the phytochemicals got from the plant source in bigger extents. Numerous bioactive auxiliary metabolites have a positive metabolic reaction on<br />different human diseases.<br />Methods: In the present examination, Artemisia nilagirica, leaves were gathered, dried, powdered and put away in hermetically sealed compartments<br />for quantitative investigation of phytochemicals according to standard strategies.<br />Results: The methanolic leaf concentrate of enrolled 4.33 mg of alkaloids, 1.22 mg of saponins, 12.4 mg of tannins, 24.3 mg of glycosides, 10.2 mg<br />terpenoids, 1.33 mg of coumarin, 59.4 mg of amino acids, 12.2 mg of fatty acids, 17.2 mg of flavonoids, 10.2 mg of phenols, and steroids in follows<br />separately.<br />Conclusion: The plant has a high helpful quality as far as an assortment of phytochemicals from leaf remove and had let to a sure level toward<br />extraction and refinement of specific bioactive mixes for human nourishment.<br />Keywords: Artemisia nilagirica, Secondary metabolites, Quantitative analysis, Leaf extract, Flavonoids.</p>

scholarly journals Biodiversity of Secondary Metabolites Compounds Isolated from Phylum Actinobacteria and Its Therapeutic Applications

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4504
Author(s):  
Muhanna Al-shaibani ◽  
Radin Maya Saphira Radin Mohamed ◽  
Nik Sidik ◽  
Hesham Enshasy ◽  
Adel Al-Gheethi ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Extreme Environments ◽  
Software Tool ◽  
Gene Clusters ◽  
Well Being ◽  
Bioactive Substances ◽  
Diverse Range ◽  
Wide Range ◽  
Potential Applications

The current review aims to summarise the biodiversity and biosynthesis of novel secondary metabolites compounds, of the phylum Actinobacteria and the diverse range of secondary metabolites produced that vary depending on its ecological environments they inhabit. Actinobacteria creates a wide range of bioactive substances that can be of great value to public health and the pharmaceutical industry. The literature analysis process for this review was conducted using the VOSviewer software tool to visualise the bibliometric networks of the most relevant databases from the Scopus database in the period between 2010 and 22 March 2021. Screening and exploring the available literature relating to the extreme environments and ecosystems that Actinobacteria inhabit aims to identify new strains of this major microorganism class, producing unique novel bioactive compounds. The knowledge gained from these studies is intended to encourage scientists in the natural product discovery field to identify and characterise novel strains containing various bioactive gene clusters with potential clinical applications. It is evident that Actinobacteria adapted to survive in extreme environments represent an important source of a wide range of bioactive compounds. Actinobacteria have a large number of secondary metabolite biosynthetic gene clusters. They can synthesise thousands of subordinate metabolites with different biological actions such as anti-bacterial, anti-parasitic, anti-fungal, anti-virus, anti-cancer and growth-promoting compounds. These are highly significant economically due to their potential applications in the food, nutrition and health industries and thus support our communities’ well-being.

scholarly journals Actinomycetes as An Environmental Scrubber

2021 ◽  
Author(s):  
Sutaria Devanshi ◽  
Kamlesh R. Shah ◽  
Sudipti Arora ◽  
Sonika Saxena
Keyword(s):  
Secondary Metabolites ◽  
Agricultural Waste ◽  
Chemical Compounds ◽  
Filamentous Bacteria ◽  
Primary And Secondary Metabolites ◽  
Sustainable Environment ◽  
Potential Source ◽  
Wide Range ◽  
Bioactive Secondary Metabolites ◽  
Broad Variety

Biotechnological tools engaged in the bioremediation process are in reality, sophisticated and dynamic in character. For specialized reasons, a broad variety of such devices are employed to produce a safe and balanced environment free of all types of toxins and so make life simpler for humans on planet Earth. Actinomycetes is one of these extremely important and functionally helpful groups. They can be used for a variety of bioremediation objectives, including biotransformation, biodegradation, and many more. Actinomycetes are one of the most varied groups of filamentous bacteria, capable of prospering in a variety of ecological settings because to their bioactive capabilities. They’re famous for their metabolic diversity, which includes the synthesis of commercially useful primary and secondary metabolites. They produce a range of enzymes capable of totally destroying all of the constituents. They are well-known for their ability to produce bioactive secondary metabolites. Members of various genera of Actinomycetes show promise for application in the bioconversion of underutilized urban and agricultural waste into high-value chemical compounds. The most potential source is a wide range of important enzymes, some of which are synthesized on an industrial scale, but there are many more that have yet to be discovered. Bioremediation methods, which use naturally existing microbes to clear residues and contaminated regions of dangerous organic chemicals, are improving all the time. In the realm of biotechnological science, the potential of actinomycetes for bioremediation and the synthesis of secondary metabolites has opened up intriguing prospects for a sustainable environment.

scholarly journals PRELIMINARY SCREENING OF PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT, AND ANTI-BACTERIAL ACTIVITIES IN THE METHANOLIC LEAF EXTRACT OF MELIOSMA SIMPLICIFOLIA (L.)

2021 ◽  
pp. 100-104
Author(s):  
PAVITHRA S ◽  
SEKAR T
Keyword(s):  
Leaf Extract ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Hot Water ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Dpph Scavenging

Objective: In the present study is investigated of phytochemicals and antioxidant activities of the leaf extracts from Meliosma simplicifolia (L.). Methods: The seaweed sample was subjected to extraction with solvents of different polarities (hot water, methanol, acetone, chloroform, and petroleum ether) and screened for phytochemicals according to standard methods. The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, and superoxide radical (O2•) scavenging activity. The ICPMS and GC-MS analysis of the methanol leaf extract of M. simplicifolia was revealed the presence of antibacterial. Results: Phytochemical analysis revealed the presence of Preliminary metabolites Quantitative studies of estimated phenol, flavonoid and tannin, as for the methanol extract of stem showed the highest content of phenolic compounds (40.71±0.94 GAE mg/100). Antioxidant activities were concluded the estimation M. simplicifolia leaf for as followed the studies. In leaf the highest DPPH scavenging activity (132.3 μg/ml), ICP-MS analysis of the leaf extract showed the presence minerals such as Mg, Fe, Cu, Na, and Zn in excess. The leaf extract of the plant was also tested for its antibacterial activity and results confirmed that it has potential activity. Conclusion: The preliminary studies in the methanol extract of the leaf of M. simplicifolia are suggestive of the antibacterial potentials of the methanol extract of leaves of M. simplicifolia.

scholarly journals Phytochemical analysis, anti-oxidant and anti-microbial activity of Acalypha indica leaf extracts in different organic solvents

2016 ◽  
Vol 8 (3) ◽  
pp. 444 ◽  
Author(s):  
Chekuri Sudhakar ◽  
Narendar Vankudothu ◽  
Shivaprasad Panjala ◽  
Nirmala Babu Rao ◽  
Roja Rani Anupalli
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Microbial Activity ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Soxhlet Extraction ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anti Oxidant ◽  
Acalypha Indica

The herb <em>Acalypha indica </em>which belongs to Euphorbiaceae family has multiple medicinal properties which include anti-oxidant, anti-bacterial, anti-fungal, anti-inflammatory, antiulcer, anti-helmintic, anti-cancerous, anti-venom, and neuro-protective activity. The present study was designed to evaluate the phytochemical, antimicrobial and anti-oxidant activity of <em>Acalypha indica </em>leaves extracts in different solvent extractions like methanol, hexane, ethyl acetate and petroleum ether. Fresh leaves of the plant were collected and shade dried. Dried leaves were milled to obtain powder. Powder was subjected to soxhlet extraction using solvents and extracts were successively obtained. Phytochemical analysis was conducted following standard methods.  Phytochemical analysis showed the presence of Alkaloids, Phenols, Saponins, Flavanoids and Amino acids. Leaf extract of methonal have shown the highest anti-oxidation capacity than hexane, ethyl acetate and petroleum ether. Anti-microbial activity has been performed on microbes like <em>Bacillus sps</em>, <em>E.coli,</em> <em>Psuedomonas sps</em> and <em>Streptococcus sps.</em> A highest value of zone of inhibition was found in methanol extract against <em>E.coli.</em> These results provide evidence that <em>Acalypha indica </em>leaf extract possesses vital phytochemicals, antimicrobial and antioxidant properties. Hence this plant can be studied further for drug analysis for finding potent medicines for diseases.

scholarly journals PHYTOCHEMICAL INVESTIGATION, GC-MS PROFILE AND ANTIMICROBIAL ACTIVITY OF A MEDICINAL PLANT RUTA GRAVEOLENS L. FROM ETHIOPIA

2017 ◽  
Vol 9 (6) ◽  
pp. 29 ◽  
Author(s):  
Henok Gulilat Azalework ◽  
Sahabjada . ◽  
Asif Jafri ◽  
Md Arshad ◽  
Tabarak Malik
Keyword(s):  
Secondary Metabolites ◽  
Leaf Extract ◽  
Micrococcus Luteus ◽  
Biological Activities ◽  
Antimicrobial Effect ◽  
Phytochemical Analysis ◽  
Ruta Graveolens ◽  
Zone Of Inhibition ◽  
Ms Analysis

Objective: This study was designed to screen the phytochemicals present in various solvents extracts of Ruta graveolens (Rue) and furthermore to investigate their antimicrobial activity.Methods: The leaves, stems and seeds of Rue were extracted using four different solvents viz. ethanolic, methanolic, chloroform, and aqueous of varying polarity. The phytochemical screening was carried out qualitatively and Gas Chromatography-Mass Spectroscopy (GC-MS) analysis was performed to identify major phytoconstituents present in the methanolic leaf extract. The antimicrobial effect of extracts was evaluated against six microbial strains namely Bacillus subtillis, Escherichia coli, Proteus vulgaris, Candida albicans, Candida tropicalis and Micrococcus luteus with disc diffusion method.Results: Phytochemical analysis revealed the presence of various secondary metabolites such as flavonoids, alkaloids, terpenoids, saponins and carotenoid. The methanolic leaf extract showed the presence of both tannin and phenolic contents in the higher amount, whereas aqueous extract displayed in the least amount. GC-MS analysis of methanolic leaf extract revealed the presence of approximately 26 phytochemical constituents. The antimicrobial assay revealed that B. subtilis showed a high zone of inhibition (20 mm) at 200 mg/ml of methanolic extract. However, E. coli and C. tropicalis did not show any zone of inhibition against each solvent extract.Conclusion: In conclusion, secondary metabolites present in the extracts have biological activities which warrant further to evaluate in vivo pharmacological studies.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF INDIGOFERA TINCTORIA L.

2018 ◽  
Vol 11 (6) ◽  
pp. 136
Author(s):  
Chandrasekaran Swaminathan
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Root Extracts ◽  
Wide Range ◽  
Indigofera Tinctoria

Objective: The present study was designed to evaluate the phytochemical composition and antibacterial and antioxidant potential of methanolic leaf and root extracts of Indigofera tinctoria L.Methods: Phytochemical analysis was done using standard methods. The methanolic leaf and root extracts of the plant were tested against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Enterobacter aerogenes, and Salmonella paratyphi B by cup-plate agar diffusion method. The free radical scavenging activities of the methanol extracts of leaves and roots were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.Results: Phytochemical screening revealed the presence of carbohydrates, reducing sugars, alkaloids, saponins, phenolic compounds, and flavonoids in methanolic leaf and root extracts. Methanolic leaf extracts of the plant exhibited antibacterial activity against a wide range of bacteria, but the root extracts failed to inhibit the tested bacterial pathogens. The antioxidant activity determination revealed that at 100 μg/ml, methanolic root extracts had the highest antioxidant activity (89.10%) on DPPH free radicals followed by methanolic leaf extracts (46.74%).Conclusion: The results of the present study conclude that the studied plant possesses broad-spectrum antibacterial and antioxidant properties and may act as a potent antioxidant for biological systems susceptible to free radical-mediated reactions. 

scholarly journals Natural Product Repertoire of the Genus Amphimedon

Marine Drugs ◽  
2018 ◽  
Vol 17 (1) ◽  
pp. 19 ◽  
Author(s):  
Nourhan Shady ◽  
Mostafa Fouad ◽  
Mohamed Salah Kamel ◽  
Tanja Schirmeister ◽  
Usama Abdelmohsen
Keyword(s):  
Secondary Metabolites ◽  
Natural Product ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Marine Sponges ◽  
Rich Source ◽  
Undescribed Species ◽  
Pharmacological Activities ◽  
Wide Range ◽  
Complete Survey

Marine sponges are a very attractive and rich source in the production of novel bioactive compounds. The sponges exhibit a wide range of pharmacological activities. The genus Amphimedon consists of various species, such as viridis, compressa, complanata, and terpenensis, along with a handful of undescribed species. The Amphimedon genus is a rich source of secondary metabolites containing diverse chemical classes, including alkaloids, ceramides, cerebrososides, and terpenes, with various valuable biological activities. This review covers the literature from January 1983 until January 2018 and provides a complete survey of all the compounds isolated from the genus Amphimedon and the associated microbiota, along with their corresponding biological activities, whenever applicable.

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