scholarly journals DETERMINATION OF LETHAL DOSE OF RICE (ORYZA SATIVA L.) GENOTYPES BY RADIO SENSITIVITY TEST

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Rigyan Gupta ◽  
Mirza Mofazzal Islam ◽  
Shamshun Nahar Begum ◽  
Wasim Akram ◽  
Md. Shafiqul Islam
Keyword(s):  
Oryza Sativa ◽  
Root Length ◽  
Oryza Sativa L ◽  
Lethal Dose ◽  
Germination Percentage ◽  
Shoot Length ◽  
Variable Response ◽  
Fresh Weight ◽  
Gamma Radiations ◽  
Radio Sensitivity

The purpose of the research was to determine the effects of gamma ray on seed germination, shoot length, root length, and shoot and root fresh weight of Rice (Oryza sativa L. spp. Indica) to identify the lethal dosage of the radiation. For this research, two rice genotypes viz., Binadhan-17 and Galon were examined for varietal differences in radio sensitivity to gamma radiations. Dry healthy seeds were figured to variable doses of gamma radiations i.e., 50-1000 Gy with 50 Gy intervals using 60Co as the radiation source. Highly significant differences among the genotypes (p<0.01) for all traits and treatments were observed. The differences among radiation treatments were highly significant (p<0.01) for germination percentage of seed, shoot and root length, shoot and root fresh weight. The genotype × dose interactions were highly significant for germination percentage, shoot length, root length, shoot fresh weight and root fresh weight indicating no stability of performance for characters across different radiation levels. Mutagenic treatments shifted mean values towards negative direction for almost all traits but not in a definite pattern. In general, genotypes displayed variable response towards gamma radiations and there LD25 and LD50 were different.

scholarly journals Rice (Oryza sativa L.) Seed Priming: It’s Effect on Seed Germination and Seedling Emergence of Spring Varieties in Kapilvastu District, Nepal

2021 ◽  
Vol 8 (2) ◽  
pp. 196-201
Author(s):  
Sabita Ghimire ◽  
Hariom Yadav ◽  
Prabesh Acharya ◽  
Raju Kharel
Keyword(s):  
Vitamin C ◽  
Root Length ◽  
Oryza Sativa L ◽  
Tap Water ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Shoot Length ◽  
Superior Performance ◽  
Vigor Index

This study aimed to determine the effect of seed priming (on-farm, hydro, halo, osmo, vitamin c priming, and hardening) on the germination and seedling growth of spring rice varieties: hardinath-3, hardinath-1, and chaite-5 at ambient room temperature. A factorial completely randomized design (CRD) was carried out with two factors: methods of priming and varieties at agriculture knowledge center (AKC), kapilbastu during march 2020. There were, in total, 21 treatment combinations and four replications. Parameters observed were germination percentage, speed of germination, vigor index, root length, shoot length, fresh weight, and dry weight of rice seedlings. Hardinath-3 had superior performance in terms of germination percentage (95.35 %), vigor index (183.86), root length (11.51 cm) and shoot length (7.39 cm) compared to hardinath-1 and chaite-5; the speed of germination, however, was greater in hardinath-1. Seed hardening - alternate soaking (tap water for 24 hours) and drying - induced higher germination percentage (94.91 %), speed of germination (96.00), and vigor index (197.43) than did other priming methods; germination percentage of vitamin c primed seeds (94.75 %), nevertheless, were at statistical par with hardened seeds. Hardened hardinath-3 seeds were better in regards to germination percentage (97.50 %) and root length (12.92 cm), while the speed of germination (97.50) and vigor index (210.50) was greater in hardened hardinath-1. Vitamin c primed seeds of hardinath-3 and hardinath-1 were finer with dry weight, shoot length, and vigor index. In conclusion, farmers' are suggested to hardened seeds of hardinath-3 before sowing to promote uniform germination and growth.

scholarly journals Growth Assessment of Tropical Sugarbeet as Influenced by Spacing

2021 ◽  
Vol 23 (2) ◽  
pp. 51-58
Author(s):  
MAT Sohel ◽  
MAE Hossain ◽  
HP Roy ◽  
SM Reza ◽  
FH Shanta ◽  
...  
Keyword(s):  
Root Length ◽  
Block Design ◽  
Dry Weight ◽  
Germination Percentage ◽  
Research Field ◽  
Shoot Length ◽  
Fresh Weight ◽  
Shoot Fresh Weight ◽  
Number Of Leaves ◽  
Maximum Root

The experiment was carried out at the research field of Agronomy and Farming Systems Division, Bangladesh Sugarcrop Research Institute (BSRI), Ishurdi, Pabna during 2012-2013 to determine the most suitable spacing for sugarbeet cultivation in Bangladesh. The experiment was conducted with nine spacing viz. 50 cm × 20 cm, 60 cm × 20 cm, 70 cm × 20 cm, 50 cm × 25 cm, 60 cm × 25 cm, 70 cm × 25 cm, 50 cm × 30 cm, 60 cm × 30 cm and 70 cm × 30 cm in a randomized complete block design with three replications. The effects of spacing on sugarbeet plantation were observed on growth and growth contributing components (germination percentage, number of leaves plant-1, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, crop growth rate) of sugarbeet. The highest germination percentage (95.67%), number of leaves plant-1 (34.33) at 30 DAS, shoot length (54.07 cm) at 120 DAS, root fresh weight (969.47 g plant-1) at 150 DAS, shoot fresh weight (752.47 g plant-1) at 120 DAS and other growth contributing parameters were obtained with the spacing 70 cm × 30 cm. However, the maximum root length (38.97 cm) was obtained with 50 cm × 20 cm spacing. It was concluded that the wider spacing promoted the growth of individual beet, though the optimum spacing for maximum root growth of sugarbeet was 50 cm × 20 cm. Bangladesh Agron. J. 2020, 23(2): 51-58

scholarly journals Effect of nitrogen and plant growth regulators on seed yield per plant and seed quality parameters in brinjal (Solanum melongena L.)

2017 ◽  
Vol 9 (4) ◽  
pp. 2338-2343
Author(s):  
Arpana D. Vaja ◽  
J. B. Patel ◽  
R.N. Daki ◽  
Shital A. Chauhan
Keyword(s):  
Seed Yield ◽  
Root Length ◽  
Seed Quality ◽  
Dry Weight ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Shoot Length ◽  
Fresh Weight ◽  
Shoot Dry Weight ◽  
Vigour Index

The present investigation on effect of nitrogen [N0 (Control), N1 (50 kg N/ha), N2 (100 kg N/ha), and N3 (150 kg N/ ha)] and plant growth regulators [G0 (Control), G1 (50 ppm GA3), G2 (50 ppm NAA) and G3 (500 ppm Cycocel)] on seed yield per plant and seed quality parameters in brinjal (Solanum melongena L.) cv. GJB 3 was carried out at the Instructional Farm, Department of Agronomy, Junagadh Agricultural University, Junagadh during kharif 2015-16. The experiment was laid out in field as per randomized block design (Factorial) with three replications. The seed harvested from 16 different treatments combinations replicated thrice from the field were analyzed in the laboratory following completely randomized design (factorial) for various seed quality parameters. Application of nitrogen @ 150 kg N/ha recorded significantly (P<0.05) highest seed yield per plant, shoot fresh weight, root dry weight, shoot dry weight and vigour index – 2 (mass), while application of 100 kg N/ha resulted in significantly (P<0.05) highest germination percentage, root length, shoot length, root fresh weight and vigour index – 1 (length). Application of GA3 at 50 ppm recorded significantly the highest seed yield per plant, germination percentage, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, vigour index – 1 (length) and vigour index – 2 (mass). Among the 16 different treatment combinations, nitrogen @ 150 kg/ha and GA3 50 ppm noted the maximum seed yield per plant, shoot dry weight and vigour index – 2 (mass), while significantly the maxi-mum germination percentage, root length, shoot length, root fresh weight, shoot fresh weight and vigour index – 1 (length) were registered in treatment combination nitrogen 100 kg N/ha and GA3 @ 50 ppm. Therefore, it is advised that application of 100-150 kg of N/ha as a nitrogenous fertilizer and spray GA3 @ 50 ppm (G1) at 45 days after transplanting helps in increasing the seed yield per plant and seed quality parameters in brinjal seed production.

scholarly journals A, The EXPLORING THE ALLELOPATHIC AND HERMETIC EFFECT OF KHATAMI (Altheae officinalis) ON EMERGENCE AND SEEDLING GROWTH OF RADISH (Raphanus sativus)

2021 ◽  
Vol 27 (3) ◽  
pp. 321-330
Author(s):  
Bilal Ahmad Khan
Keyword(s):  
Aqueous Extract ◽  
Seedling Growth ◽  
Root Length ◽  
Raphanus Sativus ◽  
Germination Percentage ◽  
Shoot Length ◽  
Fresh Weight ◽  
Germination Time ◽  
Shoot Fresh Weight ◽  
Mean Germination Time

Abstract Crop plants release biochemicals into surrounding environment that help in obtaining maximum yield of crop by acting bioherbicide at high concentration and plant growth hormone at low concentration. Therefore, this study was planned at Department of Agronomy in Weed Science Laboratory, University of Agriculture Faisalabad during Winter 2018 to evaluate the hermetic effects of Althaea officinalis L. (khatami) on vegetable crop Radish (Raphanus sativus) The experiments were laid out under completely randomized design (CRD) having three replications. The aqueous extracts of A. officinalis L. were used on radish different concentrations (2.5%, 5%, 10%, 20%, 40% and 80%). Data regarding seed germination and seedling growth (shoot length, root length, shoot fresh weight, root dry weigh) of radish were recorded using standard procedures. Results Reveals that the aqueous extract of A. officinalis at higher concentration (80%) act as bioherbicide and produced inhibitory effects on R. sativus result in Maximum time to 50% germination (4.68 days) and mean germination time (9.05 days) while minimum germination index (3.60), germination percentage (85.00%), shoot length (42.4 cm), root length (25.90 cm), shoot fresh weight (53.30 g) and root fresh weight (13.30 g). While at lower concentration (10%) it shows hermetic effect and produce maximum germination percentage (100.00%), shoot length (58.2 cm), root length (52.0 cm), shoot fresh weight (90.00g), root fresh weight (26.30 g) minimum time to complete 50% germination (3.36 days), germination index (6.00%) and mean germination time (5.73 days) growth. In A. officinalis among different phenolic compound minimum Syringic acid (0.60%) and maximum Quercetion (12.3%) compound was found. Therefore, it was concluded from this study that aqueous extract of officinalis can be used as growth promoter at lower concentration while at higher concentration for environmentally friendly control of weeds.

Responses of Weedy Rice to Drought Stress at Germination and Seedling Stages

2013 ◽  
Vol 316-317 ◽  
pp. 451-459 ◽  
Author(s):  
Guo Hua Ding ◽  
Xiao Liang Liu ◽  
Dian Rong Ma ◽  
Xiao Xue Wang ◽  
Guang Yang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Water Stress ◽  
Drought Stress ◽  
Root Length ◽  
Dry Weight ◽  
Shoot Length ◽  
Stress Index ◽  
Weedy Rice ◽  
Fresh Weight ◽  
Cultivated Rice

Drought is a world-spread problem seriously influencing grain production and quality, the loss of which is the total for other natural disasters, with increasing global climate change making the situation more serious. Rice is the staple food for more than 23% of world population, so rice anti-drought physiology study is of importance to rice production and biological breeding for the sake of coping with abiotic and biotic conditions. Much research is involved in this hot topic, but the pace of progress is not so large because of drought resistance being a multiple-gene-control quantitative character. On the other hand, stress adaptive mechanisms are quite different, with stress degree, time course, materials, soil quality status and experimental plots, thus increasing the complexity of the issue in question. Additionally, a little study is related to weedy rice.In order to investigate the effects of drought stress on germination and early seedling growth of weedy rice (Oryza sativa f. spontanea L.) and cultivated rice (Oryza sativa L.), polyethyleneglycol-6000 (PEG-6000) are used to generate -1.33MPa and 0MPa water stress in a laboratory condition (28±3°C). Complete randomized design with three replications is used in the study. After 10 days of germination, shoot length, the longest root length, root fresh weight, root dry weight, shoot fresh weight, shoot dry weight and root numbers are measured; germination percentage, and root to shoot ratio are calculated. Germination index (GI), shoot length stress index (SLSI), root length stress index (RLSI) and dry matter stress index (DMSI) are used to evaluate the response of different genotypes to PEG-induced water stress. Results of ANOVA analysis show that responses of weedy rice accessions and cultivated rice varieties to water stress are significant different, demonstrating the germplasm of weedy rice and cultivated rice are diverse which enables us to screen the germplasm tolerant to drought stress.

scholarly journals Bacillus Pumilus Induced Tolerance of Maize (Zea Mays L.) Against Cadmium (Cd) Stress

2021 ◽  
Author(s):  
Asim Shahzad ◽  
Mahmood Elahie ◽  
Muhammad Naeem ◽  
Tasmia Bashir ◽  
Humaira Yasmin ◽  
...  
Keyword(s):  
Root Length ◽  
Bacillus Pumilus ◽  
Germination Percentage ◽  
Leaf Length ◽  
Shoot Length ◽  
Maize Plant ◽  
Cd Stress ◽  
Fresh Weight ◽  
Number Of Leaves ◽  
Maize Plants

Abstract Purpose: Heavy metals contaminate the soil that alters the properties of soil and negatively affect plants growth. Using microorganism and plant can remove these pollutants from soil. The present investigation was designed to evaluate the induced effect of Bacillus pumilus on maize plant in Cadmium (Cd) contaminated soil. Methods: Three different concentrations of Cd (i.e. 0.25, 0.50 and 0.75 mg kg-1) were applied in soil under which maize plants were grown. The germination percentage, shoot length, leaf length, number of leaves, root length,fresh weight and nutrient uptake by maize plant were determined. The experiment was conducted by using complete randomized design (CRD) with three replicates. Results: The result indicated that germination percentage, Shoot length, leaf length, root length, number of leaves, and plant fresh weight were reduced by 37, 39, 39, 32 and 59% respectively at 0.75mg kg-1 of CdSO4concentration but when maize seeds inoculated with Bacillus pumilus significantly increased the germination percentage, shoot length, leaf length, number of leaves, plant fresh weight at different concentrations of CdSO4. Moreover, the plant protein were significantly increased by 60% in T6 (0.25 mg kg-1 of CdSO4+inoculated seed) and Peroxidase dismutase (POD) was also significantly higher by 346% in T6 (0.25 mg kg-1 of CdSO4+inoculated seed), however, the Superoxide dismutase (SOD) was significantly higher in T5 (0.75 mg kg-1 of CdSO4+ uninoculated seed) and was 769% higher as compared to control. The Cd contents in Bacillus pumilus inoculated maize roots and shoots were decreased. Conclusion: The present investigations indicated that the inoculation of maize plant with Bacillus pumilus can help maize plants to withstand Cd stress but higher concentration of Cd can harm the plant. The Bacillus pumilus has good potential to remediate Cd from soil, and also have potential to reduce the phytoavailibility and toxicity of Cd.

scholarly journals Effects of Ethyl methanesulfonate (EMS) on Seedling and Yield Contributing Traits in Basmati Rice

2021 ◽  
Author(s):  
Muhammad Rashid ◽  
Areeqa Shamshad ◽  
Ljupcho Jankuloski
Keyword(s):  
Oryza Sativa ◽  
Plant Height ◽  
Root Length ◽  
Oryza Sativa L ◽  
Shoot Length ◽  
Chemical Mutagenesis ◽  
Panicle Length ◽  
Ethyl Methane ◽  
Chemical Mutagens ◽  
Sterile Spikelet

Increasing genetic diversity in crop plants has been used for chemical mutagenesis. Through the application of various mutagenic agents, over 430 new varieties have been derived as rice mutants (Oryza sativa L.) Chemical mutagens such as ethyl methane sulphonate (EMS), diepoxybutane derivative (DEB), sodium azide, and gamma ray, x-ray, and quick neutron irradiation have been commonly used to induce a large number of functional variations in rice and others crops. Among chemical mutagens, ethyl methane sulfonate (EMS) is the alkylating agent most widely used in plants because it induces nucleotide substitutions to be extremely frequent, as detected in various genomes. In this study, seeds of potential genotype of the popular variety, (Oryza sativa L. Super Basmati variety) were treated with EMS at concentrations of 0.25%, 0.50%, 0.75%, 1% and 1.5%. Various measurements on the M1 generation determined EMS sensitivity. As concentration of applied EMS increased, will decrease in germination, shoot length, root length, plant height, productive tillers, Panicle Length, Total Spikelet, sterile spikelet and fertility under field conditions were observed in M1 generation as compared to the non-treatment control. Emergence, shoot length, root length, plant height, productive tillers, Panicle Length, Total Spikelet, sterile spikelet and fertility also decreased with increases in EMS mutagenesis in an approximately linear fashion. The LD50 values were observed based on growth reduction of seedlings after EMS treatment with 0.25% and 0.50% on the rice variety (Oryza sativa L. spp.).

scholarly journals Homeopathy for Treating Contaminated Cucumber Seedlings With the Herbicide Residues

2019 ◽  
Vol 11 (11) ◽  
pp. 295
Author(s):  
Ricardo A. Felito ◽  
Oscar M. Yamashita ◽  
Wagner Gervazio ◽  
Marco A. C. Carvalho ◽  
Delmonte Roboredo ◽  
...  
Keyword(s):  
Root Length ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shoot Length ◽  
The Other ◽  
Distilled Water ◽  
Percentage Germination ◽  
Fresh Weight ◽  
Significant Difference ◽  
Germination Speed

The aim of this study was to evaluate the effect of homeopathic medicines in the neutralization of waste herbicide 2,4-D+picloram in cucumber seeds, in the municipality of Alta Floresta-MT process. The experiment was carried out to evaluate two herbicide concentrations and five Nux vomica dynamizations (6CH, 12CH, 18CH, 24CH and 30CH), in addition to two controls, one with distilled water and the other contaminated with herbicide alone. The variables assessed were germination percentage, germination speed index, fresh weight, dry weight, shoot length, root length and stem thickness, where we observed a significant difference in most of the variables analyzed. From the results, it can be concluded that the homeopathic preparations in five dynamics of Nux vomica (6CH, 12CH, 18CH, 24CH and 30CH), act positively on vigor and development of cucumber seeds.

scholarly journals Bacillus pumilus induced tolerance of Maize (Zea mays L.) against Cadmium (Cd) stress

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Asim Shahzad ◽  
Mingzhou Qin ◽  
Mahmood Elahie ◽  
Muhammad Naeem ◽  
Tasmia Bashir ◽  
...  
Keyword(s):  
Root Length ◽  
Bacillus Pumilus ◽  
Germination Percentage ◽  
Leaf Length ◽  
Shoot Length ◽  
Maize Plant ◽  
Cd Stress ◽  
Fresh Weight ◽  
Number Of Leaves ◽  
Maize Plants

AbstractHeavy metals contaminate the soil that alters the properties of soil and negatively affect plants growth. Using microorganism and plant can remove these pollutants from soil. The present investigation was designed to evaluate the induced effect of Bacillus pumilus on maize plant in Cadmium (Cd) contaminated soil. Three different concentrations of Cd (i.e. 0.25, 0.50 and 0.75 mg kg−1) were applied in soil under which maize plants were grown. The germination percentage, shoot length, leaf length, number of leaves, root length, fresh weight and nutrient uptake by maize plant were determined. The experiment was conducted by using complete randomized design (CRD) with three replicates. The result indicated that germination percentage, Shoot length, leaf length, root length, number of leaves, and plant fresh weight were reduced by 37, 39, 39, 32 and 59% respectively at 0.75 mg kg−1 of CdSO4 concentration but when maize seeds inoculated with Bacillus pumilus significantly increased the germination percentage, shoot length, leaf length, number of leaves, plant fresh weight at different concentrations of CdSO4. Moreover, the plant protein were significantly increased by 60% in T6 (0.25 mg kg−1 of CdSO4 + inoculated seed) and Peroxidase dismutase (POD) was also significantly higher by 346% in T6 (0.25 mg kg−1 of CdSO4 + inoculated seed), however, the Superoxide dismutase (SOD) was significantly higher in T5 (0.75 mg kg−1 of CdSO4 + uninoculated seed) and was 769% higher as compared to control. The Cd contents in Bacillus pumilus inoculated maize roots and shoots were decreased. The present investigations indicated that the inoculation of maize plant with Bacillus pumilus can help maize plants to withstand Cd stress but higher concentration of Cd can harm the plant. The Bacillus pumilus has good potential to remediate Cd from soil, and also have potential to reduce the phyto availability and toxicity of Cd.

scholarly journals Respons Morfologis Beberapa Varietas Padi (Oryza sativa L.) terhadap Kekeringan pada Fase Perkecambahan (Morphological Response of Some Rice (Oryza sativa L.) Cultivars to Water Deficit at the Seedling Stage)

2012 ◽  
Vol 2 (2) ◽  
Author(s):  
Maria Ballo ◽  
Song Ai Nio ◽  
Feky R Mantiri ◽  
Dingse Pandiangan
Keyword(s):  
Oryza Sativa ◽  
Water Deficit ◽  
Root Length ◽  
Oryza Sativa L ◽  
Shoot Length ◽  
Seedling Stage ◽  
Seminal Root ◽  
Rice Cultivars ◽  
Morphological Response ◽  
Peg 8000

Abstrak Biji memerlukan sejumlah besar air yang harus diserap sebelum perkecambahan bisa terjadi, yaitu sekitar dua atau tiga kali dari berat keringnya. Penelitian ini menguji konsistensi respons morfologis padi terhadap kekeringan pada fase perkecambahan dengan perlakuan larutan polietilen glikol (PEG) sebagai larutan osmotikum. Penelitian ini dilakukan dengan percobaan faktorial dalam Rancangan Acak Kelompok yang terdiri dari 5 perlakuan PEG 8000 mencakup PEG 0, -0,25, -0,5, -0,75 dan -1,0 MPa terhadap 7 varietas padi, yaitu Beras Merah, IR 64, Burungan, Superwin, Serayu, Aries, dan Cigeulis. Hasil penelitian ini menunjukkan terdapat perbedaan respons morfologis terhadap kekeringan yang diinduksi dengan PEG 8000 pada fase perkecambahan di antara ketujuh varietas padi. Panjang akar seminal, panjang tunas, rasio panjang akar seminal : panjang tunas, persentase perkecambahan dan indeks vigor benih dapat dipakai sebagai indikator toleransi kekeringan yang potensial pada fase perkecambahan padi. Toleransi varietas Superwin terhadap kekeringan yang diinduksi dengan PEG 8000 pada fase perkecambahan lebih tinggi daripada varietas padi lainnya. Kata kunci: indikator, PEG, toleransi, kekeringan Abstract The seeds required a large amount of water, i.e. two or three times of their dry weight, to be absorbed before the germination. This study evaluated the consistency of morphological response of rice to water deficit at the seedling stage. The research was conducted as factorial experiment in Randomized Block Design. The treatments of PEG-8000-induced-water deficit were PEG 0, -0,25, -0,5, -0,75 and -1,0 MPa. Those treatments were applied to 7 rice cultivars, i.e. Beras Merah, IR 64, Burungan, Superwin, Serayu, Aries, and Cigeulis. The results showed the difference of morphological response to PEG-8000-induced-water deficit among 7 rice cultivars at the seedling stage. The seminal root length, shoot length, ratio seminal root length: shoot length, seedling percentage and seed vigor index were able to be used as potential water-deficit-tolerant indicators at the seedling stage. Cultivar Superwin was more tolerant to PEG 8000-induced- water deficit than 6 other rice cultivars. Keywords: PEG, indicator, water- deficit tolerant

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