scholarly journals Optimisation of a hydrolysed collagen production process from heat-treated bovine bone based on acid and enzymatic proteolysis

2020 ◽  
Author(s):  
Stephanie Angela
Keyword(s):  
Production Process ◽  
Bovine Bone ◽  
Collagen Production ◽  
Heat Treated ◽  
Enzymatic Proteolysis

scholarly journals Morphological Change of Heat Treated Bovine Bone: A Comparative Study

Materials ◽  
2012 ◽  
Vol 6 (1) ◽  
pp. 65-75 ◽  
Author(s):  
Sumit Pramanik ◽  
Asyikin Hanif ◽  
Belinda Pingguan-Murphy ◽  
Noor Abu Osman
Keyword(s):  
Comparative Study ◽  
Morphological Change ◽  
Bovine Bone ◽  
Heat Treated

Effect of Heat-Treatment Temperature on the Osteoconductivity of the Apatite Derived from Bovine Bone

2006 ◽  
Vol 309-311 ◽  
pp. 41-44 ◽  
Author(s):  
Sang Hoon Rhee ◽  
Ho Nam Park ◽  
Yang Jo Seol ◽  
Chong Pyong Chung ◽  
Sang Hyuk Han
Keyword(s):  
Heat Treatment ◽  
Physical Properties ◽  
Heat Treatment Temperature ◽  
Zealand White Rabbit ◽  
Treatment Temperature ◽  
Bovine Bone ◽  
Carbonate Ions ◽  
Heat Treated ◽  
Ft Ir ◽  
Field Emission Electron Microscopy

Effect of heat-treatment temperature on the osteoconductivity of the apatite derived from bovine trabecular bone was investigated. Three different heat-treatment temperatures (600, 800 and 1000 oC) were adopted in the experiment and their effects on the physical properties of apatite granules, which could affect on the osteoconductivity, were evaluated. The content of carbonate ions in the apatite structure was assessed by FT-IR and its crystallinity was evaluated by X-ray diffractometry. The microstructure was assessed by field emission electron microscopy. Apatite granules heat-treated at 600 oC and 1000 oC were implanted into the calvaria of New Zealand White rabbit for 4 weeks, respectively, and the undecalcified ground histologic specimens stained with multiple staining method was observed. As increasing the heat-treatment temperature, the crystal size and crystallinity of the apatite increased while the content of carbonate ions decreased. The apatite granules heat-treated at 600 oC showed much better osteoconductivity comparing to that heat-treated at 1000 oC. The results were explained in terms of the physical properties of apatite which could affect to the osteoconductivity.

Preparation of a Bioactive Xenograft

2007 ◽  
Vol 330-332 ◽  
pp. 99-102
Author(s):  
Hyung Sup Kim ◽  
Yong Keun Lee ◽  
Bum Soon Lim ◽  
Chong Pyong Chung ◽  
Sang Hoon Rhee
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Size Range ◽  
Bovine Bone ◽  
Mixed Solution ◽  
Bioactive Materials ◽  
Carbonated Apatite ◽  
Heat Treated ◽  
New Process ◽  
Low Crystalline

The method which gives a low crystalline hydroxyl carbonated apatite forming ability to originally non-bioactive high crystalline hydroxyapatite surface was newly developed. The granules of trabecular bovine bone, which had a size range from 212 to 1000 μm, were defatted, deproteinized, and then heat-treated at 1000 oC for 3 hours to remove organics completely. They were treated with the mixed solution of calcium chloride and calcium hydroxide in Soxhlet’s apparatus at 100 oC for 3 days and then dried completely. Low crystalline hydroxyl carbonated apatite was observed to occur on the surface of high crystalline hydroxyapatite granules after soaking them into the simulated body fluid (SBF) for 1 week. This method is likely to have a potential to be used as a new process to give a bioactivity to originally non-bioactive materials.

scholarly journals Osteogenic Differentiation of hDPSCs on Biogenic Bone Apatite Thin Films

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Michele Bianchi ◽  
Alessandra Pisciotta ◽  
Laura Bertoni ◽  
Matteo Berni ◽  
Alessandro Gambardella ◽  
...  
Keyword(s):  
Thin Films ◽  
Osteogenic Differentiation ◽  
Biological Properties ◽  
Bovine Bone ◽  
Crystallinity Degree ◽  
Heat Treated ◽  
Human Dental Pulp ◽  
Pulsed Electron Deposition ◽  
Bone Apatite

A previous study reported the structural characterization of biogenic apatite (BAp) thin films realized by a pulsed electron deposition system by ablation of deproteinized bovine bone. Thin films annealed at 400°C exhibited composition and crystallinity degree very close to those of biogenic apatite; this affinity is crucial for obtaining faster osseointegration compared to conventional, thick hydroxyapatite (HA) coatings, for both orthopedics and dentistry. Here, we investigated the adhesion, proliferation, and osteogenic differentiation of human dental pulp stem cells (hDPCS) on as-deposited and heat-treated BAp and stoichiometric HA. First, we showed that heat-treated BAp films can significantly promote hDPSC adhesion and proliferation. Moreover, hDPSCs, while initially maintaining the typical fibroblast-like morphology and stemness surface markers, later started expressing osteogenic markers such as Runx-2 and OSX. Noteworthy, when cultured in an osteogenic medium on annealed BAp films, hDPSCs were also able to reach a more mature and terminal commitment, with respect to HA and as-deposited films. Our findings suggest that annealed BAp films not only preserve the typical biological properties of stemness of, hDPSCs but also improve their ability of osteogenic commitment.

Effect of Heat Treating Temperature on Chemical and Physical Properties of Natural Hydroxyapatite Produced From Bovine Bone

2009 ◽  
Author(s):  
Mousa Younesi ◽  
Mehdi Javidi ◽  
Mohammad Ebrahim Bahrololoom ◽  
Hamidreza Fooladfar
Keyword(s):  
Physical Properties ◽  
Diffraction Analysis ◽  
Heat Treating ◽  
White Powder ◽  
Bovine Bone ◽  
X Ray Diffraction ◽  
X Ray ◽  
Heat Treated ◽  
Different Temperatures ◽  
Natural Hydroxyapatite

This study focused on chemical and physical properties of Hydroxyapatite powder was prepared by burning bone and heat treating the obtained bone ash at different temperatures (600, 700, 800, and 1100 °C) in an air furnace. The black ash was converted to a white powder after heat treatment. Results of X-ray diffraction analysis and Fourier transform infra-red spectroscopy that were done on heat treated powders in different temperatures indicated that the white powder was hydroxyapatite and did not contain any organic components of the bone. Furthermore, results of X-ray diffraction analysis were shown that phase transformation of the resulted hydroxyapatite to other calcium phosphate phases did not occur up to 1100 °C. X-ray fluorescence analyses revealed that calcium and phosphorous were the main elements and magnesium and sodium were present as minor impurities. The results of the energy dispersive X-ray analysis showed that Ca/P ratio of this natural hydroxyapatite varies between 1.46 and 2.01. The resulted material was found to be thermally stable up to 1100 °C. The density of natural hydroxyapatite heat treated at 800 °C was measured to be 3.187 g/cm3.

Biocompatibility Evaluation of Zeolite Compared to Bone HA, Calcium Phosphate (Ca2PO4) and Eugenol Paste

2005 ◽  
Vol 284-286 ◽  
pp. 561-564 ◽  
Author(s):  
A.P.M. Schainberg ◽  
Lutfiye Sevgi Özyeğin ◽  
P. Kursuoğlu ◽  
Patricia Valério ◽  
Alfredo Goes ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Calcium Phosphate ◽  
Optical Microscopy ◽  
Cellular Proliferation ◽  
Bovine Bone ◽  
Collagen Production ◽  
Vacuole Formation ◽  
Apoptosis Index ◽  
Bone Calcium

The biocompatibility of Zeolite was evaluated, in vitro, compared to a control and to three different biomaterials: hydroxyapatite from bovine bone, calcium phosphate and a commercial eugenol paste. The Zeolite did not affect cellular proliferation neither the alkaline phosphatase and collagen production. The apoptosis index of the zeolite groups were similar to control and optical microscopy observations did not show any morphological cell change, except the some cytoplasmatic vacuole formation.

Direct Observation of Crystalline Ordering In Naturally Graphitized Carbon Produced by Low Grade Metamorphism

1977 ◽  
Vol 35 ◽  
pp. 162-163
Author(s):  
Thomas R. McKee ◽  
Peter R. Buseck
Keyword(s):  
Crystallite Size ◽  
Sedimentary Rocks ◽  
Carbonaceous Material ◽  
Low Grade ◽  
X Ray ◽  
Graphitized Carbon ◽  
Transmission Electron ◽  
Heat Treated ◽  
Commercial Carbon ◽  
Metamorphic Zone

Sediments commonly contain organic material which appears as refractory carbonaceous material in metamorphosed sedimentary rocks. Grew and others have shown that relative carbon content, crystallite size, X-ray crystallinity and development of well-ordered graphite crystal structure of the carbonaceous material increases with increasing metamorphic grade. The graphitization process is irreversible and appears to be continous from the amorphous to the completely graphitized stage. The most dramatic chemical and crystallographic changes take place within the chlorite metamorphic zone.The detailed X-ray investigation of crystallite size and crystalline ordering is complex and can best be investigated by other means such as high resolution transmission electron microscopy (HRTEM). The natural graphitization series is similar to that for heat-treated commercial carbon blacks, which have been successfully studied by HRTEM (Ban and others).

Effect of Shock Loading on the Microstructure of Uniloy 326

1974 ◽  
Vol 32 ◽  
pp. 504-505
Author(s):  
K. P. Staudhammer ◽  
L. E. Murr
Keyword(s):  
Grain Size ◽  
Shock Loading ◽  
Current Investigation ◽  
Two Phase ◽  
05 C ◽  
Shock Deformation ◽  
Heat Treated

The effect of shock loading on a variety of steels has been reviewed recently by Leslie. It is generally observed that significant changes in microstructure and microhardness are produced by explosive shock deformation. While the effect of shock loading on austenitic, ferritic, martensitic, and pearlitic structures has been investigated, there have been no systematic studies of the shock-loading of microduplex structures.In the current investigation, the shock-loading response of millrolled and heat-treated Uniloy 326 (thickness 60 mil) having a residual grain size of 1 to 2μ before shock loading was studied. Uniloy 326 is a two phase (microduplex) alloy consisting of 30% austenite (γ) in a ferrite (α) matrix; with the composition.3% Ti, 1% Mn, .6% Si,.05% C, 6% Ni, 26% Cr, balance Fe.

Phase Transformation In Ti-6al-4v Alloys

1972 ◽  
Vol 30 ◽  
pp. 584-585
Author(s):  
Shiro Fujishiro
Keyword(s):  
Phase Transformation ◽  
Grain Boundary ◽  
Cryogenic Temperature ◽  
Β Phase ◽  
Heat Treated ◽  
Mixed Microstructure ◽  
Ductile Behavior

The Ti-6 wt.% Al-4 wt.% V commercial alloys have exhibited an improved formability at cryogenic temperature when the alloys were heat-treated prior to the tests. The author was interested in further investigating this unusual ductile behavior which may be associated with the strain-induced transformation or twinning of the a phase, enhanced at lower temperatures. The starting materials, supplied by RMI Co., Niles, Ohio were rolled mill products in the form of 40 mil sheets. The microstructure of the as-received materials contained mainly ellipsoidal α grains measuring between 1 and 5μ. The β phase formed an undefined grain boundary around the a grains. The specimens were homogenized at 1050°C for one hour, followed by aging at 500°C for two hours, and then quenched in water to produce the α/β mixed microstructure.

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