scholarly journals Variation of fruits morphometric parameters and bioactive compounds of Asimina triloba (L.) Dunal germplasm collection

10.5219/1019 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 1-7
Author(s):  
Ján Brindza ◽  
Olga Grygorieva ◽  
Svitlana Klymenko ◽  
Olena Vergun ◽  
Ján Mareček ◽  
...  

The objective of this study was to evaluate the morphological parameters and bioactive compounds (antioxidant activity, total polyphenol, flavonoid, and phenolic acid content) of 6 genotypes of dry Asimina triloba (L.) fruit from Slovak University of Agriculture in Nitra (Slovakia). Genotypes were obtained from the seeds that were sown in the year 2000. Their morphometric parameters were following: fruit weight from 59.00 to 241.19 g, fruit length from 50.14 to 140.11 mm, fruit diameter from 37.55 to 64.67 mm, number of fruits per cluster from 2 to 8, seed weight from 0.06 to 1.80 g, seed length from 16.33 to 29.11 mm, seed width from 9.56 to 18.33 mm, seed thickness from 4.98 to 9.75 mm, number of seeds in fruit from 4 to 16. The shape indexes of fruits were found ranging from 1.53 to 2.16. The variability of important is the average seeds weight from 7.40 to 35.61%, fruit weight from 14.84 to 32.95%, number of fruits per cluster from 18.21 to 32.54% and a number of seeds in fruit from 13.49 to 27.72%. The other characteristics are more or less stable. Total polyphenol content ranged from 22.13 to 37.36 mg GAE per g, total flavonoid content from 15.10 to 32.03 mg QE per g and phenolic acids content from 0.23 to 0.76 mg CAE per g. All tested samples exhibited DPPH radical scavenging activities with values from 2.84 to 7.04 mg TEAC per g. Antioxidant activity by molybdenum reducing antioxidant power method ranged from 97.25 to 275.41 mg TEAC per g of dry matter. Differences between the genotypes were significant in all observed parameters. This species is potential for propagation and practice used in the Slovak Republic.

Author(s):  
O. V. Grygorieva ◽  
S. V. Klymenko ◽  
O. N. Vergun ◽  
J. Brindza ◽  
E. Ivanišová

Pseudocydonia sinensisSchneid. (Chinese quince) fruits are an excellent source of bioactive compounds. The objective of this study was to evaluate the antioxidant activity, total polyphenol, flavonoid, and phenolic acid content of dryPseudocydonia sinensisfruits (peel and pulp) of collection fromM.M.GryshkoNationalBotanical Garden (Kyiv,Ukraine). All tested samples (peel and pulp) exhibited DPPH radical scavenging activities with values from 9.31 to 10.23 and from 5.39 to 6.85 mg TEAC/g (trolox equivalent), respectively. Antioxidant activity determined by the molybdenum reducing antioxidant power method was ranged from 122.0 to 219.31 (peel) and from 62.19 to 95.44 (pulp) mg TEAC/g of dry matter (DM). The total polyphenol content of peel and pulp was ranged from 53.17 to 78.67 and from 32.31 to 47.41 mg GAE/g (gallic acid equivalent), respectively. The total flavonoid content was ranged from 9.15 to 26.18 (peel) and from 0.55 to 0.87 mg QE/g (pulp) (equivalent to quercetin). The phenolic acids content of peel and pulp fruits was ranged from 4.40 to 8.39 and from 1.12 to 3.97 mg CAE/g (caffeic acid equivalent), respectively. The results showed that peel extracts had the highest content of bioactive compounds compared with pulp extracts. Differences between the genotypes were significant in all observed parameters. The polyphenol content presents high positive correlations between antioxidant activity and phenolic content, flavonoid, and phenolic acid. This species is potential for propagation and practice use inUkraine.


2021 ◽  
Author(s):  
Noppol Leksawasdi ◽  
Siraphat Taesuwan ◽  
Trakul Prommajak ◽  
Charin Techapun ◽  
Rattanaporn Khonchaisri ◽  
...  

Abstract Green soybean (Glycine max L.) pods (GSP) are agro-industrial waste from the production of frozen green soybean and milk. These pods contain natural antioxidants and various bioactive compounds that are still underutilized. Polyphenols and flavonoids in GSP were extracted by ultrasound techniques and used in antioxidant fortification of green soybean milk. The ultrasound extraction that yielded the highest total polyphenol content was 50% amplitude for 10 min, whereas maximum flavonoids content was obtained at 50% amplitude for 15 min. Radical scavenging activity assayed by 2,2-diphenyl-1-picryl-hydrazyl radical (DPPH) and ferric reducing antioxidant power (FRAP) methods did not differ significantly (p>0.05) between the two conditions. Response surface methodology was applied to analyze an optimum ultrasonic-assisted extraction (UAE) condition of these variables. The highest desirability was found to be 50% amplitude with extraction time of 12.5 min. Fortification of the GSP extracts (1-3% v/v) in green soybean milk resulted in higher levels of bioactive compounds and antioxidant activity in a dose-dependent manner. Procyanidins were found to be the main polyphenols in dried GSP which were present at the concentration of 104.1 ± 2.1 mg/g dry sample, respectively. Addition of GSP extracts obtained by using an ultrasound technique to green soybean milk increased its bioactive compound content especially procyanidins as well as its antioxidant activity.


Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 620
Author(s):  
Mihaela Mirela Bratu ◽  
Semaghiul Birghila ◽  
Antoanela Popescu ◽  
Bogdan-Stefan Negreanu-Pirjol ◽  
Marius Radu ◽  
...  

Using two methods (ferric reducing antioxidant power and radical scavenging activity), the total polyphenol content (Folin–Ciocalteu reagent) and polyphenol patterns (HPLC) in 10 commercial lager beer brands produced in Romania was determined. Samples bottled in glass, plastic and aluminium packages were analysed for each brand when available. Results have indicated considerable variations in the total and individual phenolic contents as well as antioxidant activity across beer brands. Gallic and feulic acids were the dominant phenolic compounds identified samples. The statistical analysis indicates a significant difference between the levels of antioxidant activity detected using DPPH and FRAP, for all three types of material considered. The statistical test ANOVA indicates that the polyphenol concentration is similar across all types of material.


Author(s):  
NAGENDAR SHETTY ◽  
INDU

Objective: Prunus cerasus L. is consumed as a dietary supplement to promoting health benefits. The purpose of this study was to investigate the phenol and flavonoids content of sour cherry methanol extract in terms of antioxidant activity. Methods: The content of polyphenols and flavonoids was quantified. DPPH and FRAP assay were used to evaluate the radical scavenging properties and reducing the antioxidant power of sour cherry fruit. Results: DPPH scavenging activity was evaluated IC50 10.32±2.23 mg/ml and FRAP was estimated at 205±30 µmol/g. Conclusion: Sour cherry methanol extract includes various bioactive compounds like polyphenols, flavonoids and anthocyanidins, which can be valuable beneficial effects to the prevention of various vascular diseases.


2013 ◽  
pp. 269-277 ◽  
Author(s):  
Zoran Maksimovic ◽  
Nebojsa Nedic

Antioxidant properties of fifteen multifloral and honeydew types of honey from Serbia were assessed by determination of ferric-reducing antioxidant power (FRAP assay) and 2,2- diphenyl-1-picrylhydrazyl free radical scavenging ability (DPPH assay), with respect to their total polyphenol content and colour intensity. The results of this study showed that total polyphenol content, antioxidant activity and colour intensity varied widely among different samples, even within the same type of honey. All investigated parameters were strongly dependent on geographic origin of samples, further emphasizing the importance of their detailed chemical characterisation. In general, polyphenolic content in investigated samples of honey, expressed as catechin equivalents, ranged from 480.2 to 1861.1 mg/kg. The ferric-reducing antioxidant power of honey tended to be lower in brighter and higher in darker samples, varying between 489.6 and 3089.8 ?mol Fe(II) per 100 g of honey. The correlation between the colour and antioxidant activity never reached statistical significance in the case of honeydew type of honey, which was opposed to a trend observed in the case of multifloral honey.


Author(s):  
Kouogueu Seuyim Ghislain ◽  
Nguedjo Wandji Maxwell ◽  
Dibacto Kemadjou Ruth Edwige ◽  
Nseme Mboma Yves Didier ◽  
Djouka Nembot Pelagie Marcel ◽  
...  

Aims: The cashew tree (family Anacardiaceae) grows widely in many parts of African countries, including Cameroon. Its fruit and nut are used for food and several studies have shown their beneficial effects on health. This work aimed to evaluate the impact of two drying methods on the content of bioactive compounds and antioxidant activity. Methodology: Four varieties (VAR 1, VAR 2, VAR 3, VAR 4) of cashew apple samples were collected and drying using sun-dried and oven-dried to a constant weight, and then ground in a blender to a powder, the fresh one was cut up and crushed in a blender. All sample were reconstituted with distilled water and polyphenols, flavonoids, alkaloids contents, and antioxidant activity through different mechanisms (DPPH radical, FRAP and TAC assays) were assessed. Results: Alkaloids ranged from 1.50 mg EQui/g MF to 5.69 mg EQui/g DM for fresh and oven-dried VAR 1 respectively, polyphenols ranged from 786.15 mg EAG/g MF to 2836.92 mg EAG/g DM for fresh and oven-dried VAR 1 respectively, flavonoids ranged from 8.18 mg EAG/g MF to 295.45 mg EAG/g DM for fresh and oven-dried VAR 2 respectively. TAC values ranged from 13.09 mg EAA/g MF to 67.06 mg EAA/g for fresh and oven-dried VAR3 and VAR2 respectively. The highest DPPH radical scavenging value (86.25%) was obtained with fresh VAR 4 and the lowest (25.67%) with fresh VAR 1. The highest ferric reducing antioxidant power (FRAP) was obtained with fresh VAR 1 and VAR 3 (0.27 mg AAE/g MF) and the lowest with VAR 3 and VAR 4 oven-dried (0.23 mg AAE/g MF). Conclusion: In conclusion, the different cashew varieties studied in this work are a good source of antioxidants. The drying method significantly affects bioactive compounds and antioxidant activities. A weak but not significant correlation was obtained between the number of bioactive compounds and antioxidant activities.


Author(s):  
Supreena SRISAIKHAM

Aqueous extracts of whole alfalfa plants (AP) and alfalfa pellets (APe) were examined for nutritional values, bioactive compounds (total phenolic content (TPC), phenolic compounds (PC), total flavonoids content (TFC), flavonoids compounds (FC), isoflavones), amino acids (AA) and antioxidant activity of AP and APe assessed via a DHHP radical scavenging assay and ferric reducing antioxidant power (FRAP) assay. The potential ingredients in the form of AP and APe were used to evaluate the nutritional values measured by chemical composition, and to investigate bioactive compounds, AA and antioxidant activity to further contribute to use as a feed ingredient or application as a high-value alternative feed supplement in the ruminant diet. Crude protein and tannins were higher in the APe (18.17 and 3.08 %) than the AP (16.21 and 1.80 %). Higher content of organic matter, crude fiber, neutral-detergent fiber, acid-detergent fiber, acid-detergent lignin was observed for AP than APe. The TPC and TFC were more abundant in AP than in APe and AP also showed stronger antioxidant activity via DPPH than APe, with positive correlations of TPC, TFC and DPPH. The highest values of gallic acid in terms of PC and apigenin in terms of FC of APe were 215.30 and 2278.27 µg/g dry weight (DW), respectively, while the highest levels of genistein and daidzein occurred in AP at 104.37 and 130.91 µg/g DW, respectively. The total of essential AA was found to be in vary the range of 9.33 to 16689.47 µg/g DW in different forms of samples, with valine as the highest (5451 µg/g DW) in APe, followed by phenylalanine (4.30 µg/g DW) in AP.


Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3701
Author(s):  
Gabriela Viana da Silva ◽  
Bruna Aparecida Souza Machado ◽  
Walkia Polliana de Oliveira ◽  
Camilla Fernanda Godinho da Silva ◽  
Cedenir Pereira de Quadros ◽  
...  

The effects of the drying process using the conventional oven and freeze-drying on the thermogravimetric profile, proximate composition, color parameters, individual bioactive compounds, and antioxidant activity in the grape residue (skin) were evaluated. Twenty individual phenolic compounds were identified, where a variation in concentration was observed for flavonols, stilbenes, phenolic acids, flavonoids, procyanidins, and particularly anthocyanins (malvidin-3,5-di-O-glucoside of 253.2–536.9 mg/kg) due to the drying process. Drying in a conventional oven caused a decrease of 23% of the total polyphenols. The skin of the BRS magna grape has a high concentration of total phenolic content of 489.5–148.3 mg.GAE/100 g, total anthocyanin content of 124.9–260.1 mg.CE/100 g, and total flavonoid content of 12.7–26.0 mg.QE/100 g. The results of free radical scavenging activity (1.26–4.91 μg/mL, as EC50) and ferric reducing antioxidant power (82.93–184.10 μmol/g of skin as equivalent to Fe2SO4) indicate high antioxidant activity, independently of the drying process applied. It was concluded that, if the application is directed to anthocyanin compounds, the use of lyophilization is recommended. On the other hand, if the interest is in bioactive compounds that exert antioxidant activity, conventional oven-drying can be used.


Author(s):  
Mabintou Diomande ◽  
Kouassi Hubert Konan ◽  
Yapo Thierry Monnet ◽  
Kouassi Armand Kanga ◽  
Jean Bedel Fagbohoun ◽  
...  

Aims: During the industrial processing of mango, considerable quantities of peel and seeds are rejected, which results in a significant economic loss for the manufacturer, as well as an impact on the environment. However, mango almond and peel flours present enormous nutritional and especially therapeutic potentialities. Thus, the objective of this work is to contribute to the valorization of the waste of 4 varieties of mango (Amelie, Kent, Keitt, Brooks) from north in Ivory Coast by the determination of their bioactive compounds in, order to be used in the pharmaceutical industry. Place and Duration of Study: Laboratory of Biochemistry and Food Technology, Nangui Abrogoua University, Abidjan 02, Ivory Coast. Between March 2019 to July 2021.  Methodology: Phenolic compounds of the Mango almond and peel flours were extracted with ethanol. UV-VIS spectrophotometry was employed to further quantify the total phenolic, tannin and total flavonoid content. DPPH radical scavenging assay, 2.2-azino-bis (3-ethylbenzthiazoline-6- sulphonic acid (ABTS) scavenging test and ferric reducing antioxidant power (FRAP) were used to determine antioxidant activity. As for phenolic acids, they were analysed by HPLC. Well diffusion method was used to determine antibacterial activity. Results: The analysis of the bioactive compounds of these mango discards revealed that they are characterised by high contents of total polyphenols 39.67 ± 0.04 to 85.18 ± 0.01 mg/g in the peel and 22.86 ± 0.03 to 58.43 ± 0.00 mg/g in the almond, flavonoids 4.36 ± 0.03 to 20.43 ± 0.02 mg/g in peel and from 6.59 ± 0.01 to 26.72 ± 0.02 mg/g in almonds, tannins 5.04 ± 1.13 to 8.64 ± 0.76 mg/g in peel and from 6.58 ± 0.06 to 12.46 ± 0.11 in almonds. Antioxidant activity varies in peel and almond from 64.49 ± 2.56 to 96.40 ± 0.32 % and 89.16 ± 1.45 to 97.96 ± 0.25 % respectively for ABTS, from 59.51 ± 0.26 to 86, 27 ± 0.56% and 80.39 ± 0.56 to 87.21 ± 0.39% for DPPH inhibition and from 0.59 ± 0.0 to 0.72 ± 0.01 mg/g and from 0.81% to 0.92% mg/g for iron reduction by the FRAP method. As for the antimicrobial activity, very marked inhibition diameters were observed both with the peel extracts and in the almonds for different bacteria (B. ceurus, E. coli, ST. aureus, S. typhi, P. aeruginosa). Conclusion: These results demonstrate that the antimicrobial and antioxidant activities of the by-products of the four mango varieties are potential sources of bioactive compounds. These by-products could therefore be used in the pharmaceutical industry and diet.


2019 ◽  
Author(s):  
Chem Int

Verbena officinalis Linn is a traditionally known medicinal plant which is used against a number of diseases including inflammatory conditions. In this study its antioxidant activity (reducing powers, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities), ferric reduction activity potential (FRAP), total flavonoid concentration and antimicrobial activities of 80%, 90%, 100% methanol and chloroform extracts of V. officinalis Linn root and 90% and100% methanol leaf extracts were determined. Its antioxidant activity increases with increase in amount of extract (10% to 40%v/v). Total flavonoid content (TFC) varied from 73.32±0.002 mgQE/100g of dry weight (90% methanol) to 42.39±0.032 mgQE/100g dry weight (chloroform), 2,2-diphenyl-1-picrylhydrazyl (DPPH), radical scavenging activity (%) was varied between 87.39% (90% methanol) to 45.57% (chloroform) while Ferric reducing antioxidant power was observed between 372.93±0.04 mgAAE/100 g extract (90% methanol) to 129.41±0.026 mgAAE/100 g chloroform in the root extract. The methanolic extract of the leaf showed less antioxidant activity than the methanolic extract of the root. Crude extracts of V. officinalis root showed various degree of antimicrobial activity towards drug resistance microbial pathogens. Growth inhibition tests against bacterial pathogens demonstrated concentration dependence. Moreover, gram positive bacteria were more susceptible to V. officinalis root extract when compared to gram negative bacteria. In general V. officinalis root and leave extracts possess strong antioxidant and antimicrobial activities.


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