scholarly journals FUNGAL COMPOSITION AND PROXIMATE ANALYSIS OF POULTRY FEEDS SOLD IN BENIN CITY, NIGERIA

2021 ◽  
Vol 2 (2) ◽  
pp. 109-115
Author(s):  
A. S. Ogbebor ◽  
A. A. Imoni ◽  
O. R. Ohiorenoya

Poultry farming contributes significantly to poverty alleviation by providing employment opportunities to Nigerians. Feed quality is the most prominent challenge of the poultry sector. This study was aimed at assessing the fungal and proximate qualities of poultry feeds sold in Benin City, Nigeria. Poultry feed samples (starter, grower and finisher) were obtained from five stores in Benin City. Enumeration and isolation of fungi was carried out using the spread plate method on potato dextrose agar and Aspergillus flavus and parasiticus agar (AFPA). Identification of fungal isolates was based on cultural and morphological characteristics.  Proximate composition was determined by standard methods. The total fungal counts of starter, grower and finisher feeds ranged from 0.10 – 8.50 x 105 sfu/g. Fungal species isolated were Aspergillus flavus, Aspergillus niger, Penicillium spp., Saccharomyces spp., Trichoderma spp., Rhizopus spp. and Mucor spp. The results from proximate analysis were as follows: 36.50±0.92 - 70.92±0.51% (protein), 3.53±0.04 - 8.45±0.16% (fibre), 11.61±0.72 - 13.91±0.27% (fat), 2.70 - 8.60% (moisture content) and 3.65 - 37.83% (carbohydrate). The temperature and relative humidity of storage ranged from 33.90oC - 35.20oC and 69% - 75% respectively. Results showed that poultry feeds sampled have diverse fungi present in them. Control measures should be adhered to in poultry industries and stores to prevent microbial contamination.

2016 ◽  
Vol 13 (1) ◽  
pp. 79-86
Author(s):  
MM Rahman ◽  
MS Islam ◽  
MZ Alam ◽  
M Ashrafuzzaman

The Experiments were carried out during the period of July 2001 to December 2002. Infestation of poultry feeds by fungi and aflatoxins content in feeds were determined. Ten feed samples were collected from ten different farms located in three different areas of Bangladesh. Five different fungal species belonging four genera were detected which were Aspergillus flavus, Aspergillus niger, Fusarium spp, Rhizopus stolonifer and Penicillium spp. Among them Aspergillus flavus and Aspergillus niger were found in all the feed samples and the rest were occasionally found. Effect of storage and moisture content of feeds were studied for determining the influence on occurrence of fungal flora in feeds. Higher the storing period of feed along with higher moisture content resulted superior incidences of fungal population in stored feeds. Studied on storage practice with 4 duration of F1 sample revealed that the presence of aflatoxins were 30.52ppb, 33.36ppb, 37.32ppb and 48.35ppb of 5, 10, 15 and 20 days after production of feeds, respectively.J. Bangladesh Agril. Univ. 13(1): 79-86, June 2015


2013 ◽  
Vol 2 (2) ◽  
pp. 128 ◽  
Author(s):  
Adeniran Lateef Ariyo ◽  
Makun Hussaini Anthony ◽  
Muhammad Hadiza Lami

In Nigeria, concentrated poultry feed is a vital component of animal production and health but there was no reliable mycogram in Niger state, Nigeria. As a result this study determined the incidence of mycotoxigenic fungi in both commercial and privately milled concentrated poultry feeds. A total of 100 poultry feed samples consisting of 52 privately milled poultry feed and 48 commercial feed samples were collected. Mycoflora in the feed was determined. Nine fungi genera were isolated. The most frequently isolated fungi genera in both privately milled and commercial feed was <em>Aspergillus</em> <em>spp </em>which was about 40% of mould isolate. <em>Penicillium spp </em>is 20% in private feed and 13% in commercial feed. A total of 874 fungi were isolated consisting of 458 fungi species in privately milled feed and 416 fungi species found in commercial feed. Mycotoxigenic fungi genera, <em>Aspergillus, Penicillium, Fusarium and Alternaria</em> were isolated. <em>Aspergillus flavus</em> is the commonest isolated fungi species.


2018 ◽  
Vol 34 (2) ◽  
pp. 239-249 ◽  
Author(s):  
Vesna Krnjaja ◽  
Slavica Stankovic ◽  
Milos Lukic ◽  
Nenad Micic ◽  
Tanja Petrovic ◽  
...  

This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Sumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Sumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Sumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Sumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Sumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 ?g kg-1) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain.


2019 ◽  
Vol 28 ◽  
pp. 59-68
Author(s):  
S Islam ◽  
N Tanjila ◽  
MF Begum

The present investigation has been carried out to assess the microbial safety and pathogenic potentialities of enterobacteria in poultry feeds. From the results it was observed that total aerobic plate count of poultry feeds samples were recorded as 2.8 × 105 to 5.8 × 109 cfu/g and 100% samples contained ≥106 cfu/g while the highest mean of cfu was counted as log10 8.797/gm. Large number of coliforms were recorded in different poultry feed samples and the ranges of cfu were counted as 1.2 × 104 to 5.2 × 107/g while average 75% samples were contaminated with coliform bacteria with ≥104 cfu/g and the highest mean of cfu was counted as log10 6.103/g. The ranges of cfu of Escherichia coli were 1.03 × 102 to 1.09 × 105/g and 70% samples contained ≥102 cfu/g while the highest mean of cfu was counted as log10 4.493/gm. But the ranges of cfu of total Salmonella sp. were recorded as 1.02 × 101 to 5.25 × 104/g and 50% samples contained ≥102 cfu/g and the highest mean of cfu was counted as log10 3.665/g. Total 29 enterobacterial isolates were isolated from the feed by using selected media. On the basis of morphological characteristics and biochemical test results the isolates were identified as Salmonella sp., Shigella sp., Klebsiella sp., Citrobacter sp., Proteus sp., Enterobacter sp. and Escherichia coli. These isolates were tested on blood agar medium and only seven isolates showed positive β-hemolytic activity.In virulence efficacy test, only hemolytic positive isolates were ingested to chicken and observed that E. coli (SGE-1), Klebsiella sp. (SSE-6) and Salmonella sp. (JSS-9) isolates were highly toxic because the experimental chickens were died after 3 days of ingestion of the bacteria, two isolates showed loose motion symptom after 15 days while other isolates showed little sickness. All the selected isolates showed positive hem-agglutination reactivity in poultry RBC. The results indicate that the poultry feeds were highly contaminated with pathogenic enterobacteria which are risk to public health. J. bio-sci. 28: 59-68, 2020


1993 ◽  
Vol 56 (3) ◽  
pp. 246-248 ◽  
Author(s):  
V. SANCHIS ◽  
A. SANCLEMENTE ◽  
J. USALL ◽  
I. VIÑAS

The predominant fungal species present in 60 samples of barley collected in Spain were Alternaria alternata, Penicillium spp. and Aspergillus flavus. Of the 176 Alternaria isolates examined, 88.6% produced tenuazonic acid, 15.3% produced alternariol, and 9% produced alternariol monomethyl ether. Only 6% of the 190 isolates of A. flavus produced aflatoxin.


1970 ◽  
Vol 6 (2) ◽  
pp. 245-251 ◽  
Author(s):  
MAM Ara ◽  
ML Khatun ◽  
M Ashrafuzzaman

Five varieties of diseased onion samples, four local (Taherpuri, Faridpuri, Kalashnagari, and Zitka) and one Indian (Pusa Red) were collected from different markets of Mymensingh, to isolate and identify the associated fungi. Five different fungal species belonging to three genera were repeatedly isolated. Healthy looking and diseased onions were kept at room temperature. The population of associated fungi increased proportionately with the duration of storage period. The isolated fungi were Aspergillus niger, Aspergillus flavus, Penicillium spp., Fusarium oxysporum and Fusarium moniliforme. Healthy onion bulbs were inoculated (with injury and without injury) with these fungi. Individual fungi produced distinguishable symptom of disease in incubation chamber at 22-23°C temperature. After 30 days of inoculation, bulbs showed comparatively low amount of disease development than those at 60 and 90 days. Maximum disease development was found in the variety Pusa Red (with injury) and it was minimum in Zitka variety (without injury). Keywords: Fungi; Onion; Disease development DOI: 10.3329/jbau.v6i2.4818 J. Bangladesh Agril. Univ. 6(2): 245-251, 2008


2021 ◽  
Vol 6 (3) ◽  
pp. 228-234
Author(s):  
Debasmita Ghosh Dhar ◽  
Priyanka Dhar ◽  
Ashok Kumar Das ◽  
Naim Uddin

Fungal spores contribute significant concentration in the bioaerosol of various environmental conditions which may have potential health threats. Our study was aimed at determining the presence of disseminating airborne fungi in a pediatric government hospital in Kolkata. The study was started from the post-monsoon to the middle winter (August to December 2008) in the indoor and outdoor environment of the hospital with temperature and humidity ranges of 11.2 °C-35.2 °C and 70 per cent-90 per cent, respectively. Air sampling was performed at 14 days intervals during the daytime following the gravitation settling method, and the fungal colonies were identified based on micro and macro morphological characteristics. The percentage contribution of individual fungal species from the outdoor section and indoor units (Newborn Baby Ward, Respiratory Care Unit, Step Down Ward, Thalassemia Care Unit) of the hospital environment was calculated. We observed profound aeromycofloral diversity where the outdoor environment was mostly colonised by sterile hyphae (16.43 %) along with the allergenic Aspergillus fumigatus (13.6 %) and Penicillium sp. (12.32 %). Conversely, an abundance of Cladosporium herbarum (24.7 %) and Penicillium sp. (17.85 %) followed by Aspergillus sp. (12.9 %) and sterile hyphae (14.51 %) were found in different indoor units. Our results showed the diversity of airborne mycoflora which promotes the trend to health difficulties and thus the hospital environment monitoring along with proper control measures is essential.


Author(s):  
Zeinab Hosseini ◽  
Mohammad Taghi Ghaneian ◽  
AbbAsali Jafari Nadoushan ◽  
Mohammad Hassan Ehrampoush ◽  
Seyedeh Mahdieh Namayandeh ◽  
...  

Introduction: By increasing the amount and diversity of bio-environmental wastewater pollutants, it is essential to be controlled and planed the environmental contaminants to eliminate or minimize. Cardboard recycling industry wastewater has the potential of environmental pollution; it is contaminated with various fungal species that may be effective in the decomposition and removal of pollutants. The aim of this study was the isolation and identification of native fungal species that decontaminate organic pollutants in the cardboard recycling industry wastewaters. Methods: In this study, which is a descriptive study, samples were randomly taken from different area of wastewater of cardboard recycling industry. Samples were cultured on Saboraud dextrose agar medium for isolation of probable fungal species, which decomposers of wastewater. The isolated fungal colonies were enumerated and purified based on morphological characteristics and microscopic analyzing as far as possible. Results: The average count of the total number of fungi species in wastewater of cardboard recycling industry was 2.5×102 CFU/ml of these 2.1×101 were saprophytic fungi. The most prevalent fungal species were identificated from the wastewater include Aspergillus niger, Aspergillus flavus, Penicillum, Geotrichum, Mucor, Drechselera, Paecillomyces, Alternaria, Fusarium and Absidia. The highest frequency of fungal species Aspergillus nige, Aspergillus flavus and Penicillum were 30.89%, 22.76% and 14.63% respectively. Conclusion: It seems that native fungi isolated from the cardboard recycling industry wastewater in this study are from fungi that have the potential for bioremediation of wastewater contaminants based on scientific literature and can be used for evaluating of their ability to decompose wastewater from this industry.  


2021 ◽  
Vol 9 (07) ◽  
pp. 799-804
Author(s):  
Parwez Qayum ◽  
◽  
J.D. Shaikh ◽  

Fungi are the core components of soil microorganisms, accounting for more of the soil biomass than bacteria, depending on soil depth and nutritional requirements. In the current study, phosphate solubilizing fungi were isolated from the soil of Maharashtras Marathwada area, and all fungal isolates were examined for their ability to solubilize phosphate.Only 11 fungal isolates out of a total of 40 were found to have P-solubilizing activity. After 48 hours of incubation, the fungal isolates Aspergillus niger (PQ9), Trichoderma spp (PQ36), and Penicillium spp (PQ19) demonstrated a considerable zone of solubilization with 34, 31 to 30 mm on selective agar medium. The potent phosphate solubilizing fungi were identified in 18SrRNA analysis.The study, therefore, proposed that these fungal species have strong phosphate solubilizing properties and can be used for excellent crop productivity as a biofertilizer.


2018 ◽  
Vol 12 (5) ◽  
pp. 5-10
Author(s):  
Seyed Soheil Ghaemmaghami ◽  
◽  
Hossien Nowroozi ◽  
Hossien Nowroozi ◽  
Mahmoud Tohidi moghadam ◽  
...  

Background: Much attention has been paid to poultry feed processing and the contamination In Iran in order to improve the production and reduce the waste. No information is available on the fungal contamination and the strains found in processed (pellet) and non-processed (mash) poultry feeds. This study was designed to determine the hygienic condition and the risk of fungal contamination affecting the quality of poultry feeds (mashed vs. pellet). Methods: A total of 90 samples of poultry feeds were collected from warehouses in Tehran and Alborz provinces. Samples were cultured on SDA, the CFUs were calculated, and the taxonomic identification of various fungal genera was made, both macroscopically and microscopically. Results: Total mould counts for mashed feeds (15×103) was significantly (P<0.05) higher than pelleted feeds (11×102). The most frequent fungal genus recovered were Fusarium spp (90%) and Aspergillus spp (70%) in mashed and pellet feeds, respectively. Conclusion: Heat processing reduced fungal contamination in poultry feeds. However, some fungal species are able to survive heat exposure and continue to form spores. We concluded that the assessment of fungal contamination in poultry feeds deserves a high attention to improve the quality, hygiene and safety of the foods originated from poultry .


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