Antioxidant Assay with Scavenging DPPH Radical of Artocarpus anisophyllus Miq Stem bark extracts and Chemical compositions and Toxicity Evaluation for the Most Active Fraction

2021 ◽  
pp. 2819-2823
Author(s):  
Erwin Erwin ◽  
Djihan Ryn Pratiwi ◽  
Iwan Saputra ◽  
Alimuddin Alimuddin
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Stem Bark ◽  
Artemia Salina ◽  
Toxicity Tests ◽  
Chemical Compositions ◽  
Test Results ◽  
Dpph Radical ◽  
Active Fraction

The purpose of this study was to determine the antioxidant activity of crude extract and their fractions, the content of secondary metabolite types of crude extract, and the chemical content and toxicity of the most active fractions as antioxidants from the stem bark of Artocarpus anisophyllus Miq. (Mentawa). The methods used are phytochemical screening, toxicity tests against Artemia salina by the Brine Shrimp Lethality Test (BSLT), antioxidant activity assay with DPPH radical reduction method, and determination of chemical compound by GC-MS analysis. Phytochemical test results showed that crude extract containing alkaloids, triterpenoids, phenolics and flavonoids. The results of the antioxidant activity of the n-hexane, ethyl acetate and methanol fractions obtained IC50 values of 127.69, 28.65 and 79.43ppm, respectively. Ethyl acetate (as the most active fraction) was then fractionated using a vacuum chromatography column and the fractions obtained were E1 (268.8mg), E2 (337.1), E3 (234.3mg) and E4 (431.2mg). The antioxidant activity test showed that E2 was the most active compared to other fractions with an IC50 value of 37.24ppm. While the toxicity test results showed that E2 was very active with an LC50 value of 6.23ppm indicating that E2 was also potentially developed as an anticancer drug. Based on GC-MS spectrum analysis, of the several main compounds, four of which are aromatic compounds that have the potential to be developed as antioxidants, namely 2- (Benzyloxy) phenol (phenolic compound) (3.96%) (51),), 1, 2- Benzenedicarboxylic acid, dinonyl ester (2.44%) (52), Linderazulene (2.43%) (30), p-Nonylphenol (2.36%). Some other aromatic minor compounds can also be active as antioxidants.

scholarly journals PENENTUAN POTENSI EKSTRAK KULIT BATANG TUMBUHAN SIKKAM (Bischofia javanica Blume) SEBAGAI ANTIOKSIDAN DENGAN METODE DPPH DAN SITOTOKSIK DENGAN METODE BSLT

Alotrop ◽  
2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Dapot Parulian Manurung ◽  
Agus Sundaryono ◽  
Hermansyah Amir
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Anticancer Agent ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Bark Extract ◽  
Test Results ◽  
Bischofia Javanica ◽  
Ethanol Fraction

This study aims to determine the level of antioxidant activity of Sikkam bark extract (Bischofia javanica Blume) and its potential as an anticancer agent. This research was conducted in November 2018 - April 2019 in the Laboratory of Chemical Education Guidance and Counseling and Basic Science Laboratory, University of Bengkulu. The sample in this study was obtained from Gunung Mariah village in Simalungun district, North Sumatra. The method used in this study was extraction using maceration, liquid-liquid fractionation, phytochemical test, DPPH (Diphenylpicrylhiydrazyl) test and BSLT test (Brine Shrimp Lethality Test). Extraction maceration using ethanol 96%, fractionation using a solvent n-hexane and ethyl acetate, phytochemical tests using Mayer's reagent, Lieberman-Burchard, FeCl3 1% and H2SO4 2N, antioxidant test using DPPH (1,1-diphenyl-2-picrylhydrazyl) and cytotoxic tests using shrimp Artemia salina Leach larvae. The results of the study on maceration of 800 grams of Sikkam bark obtained a crude extract of 53.6 grams (6.7%). Fractionation from 10 grams of crude extract obtained 7.24 gram ethanol fraction (72.4%), ethyl acetate fraction 2.14 gram (21.4%) and n-Hexane fraction 0.39 gram (3.9%). Phytochemical test results obtained the content of alkaloids, flavonoids, tannins, saponins and terpenoids with the highest yield on tannin compounds. The antioxidant activity test results obtained IC50 values in crude extracts of 20.94 ppm, ethanol fraction 36.29 ppm, ethyl acetate fraction 83.28 ppm and n-Hexane fraction 39.13 ppm which showed that crude extract, ethanol fraction, n-Hexane fraction were categorized as very strong antioxidants and Ethyl acetate fraction is categorized as a strong antioxidant. The cytotoxic test results using crude extract obtained an LC50 value of 54,827 ppm which showed that Sikkam bark extract was toxic so that it was potential as an anticancer agent.

scholarly journals ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK

2019 ◽  
Vol 19 (3) ◽  
pp. 58-63
Author(s):  
Ilham Maulana ◽  
Binawati Ginting ◽  
Nurdin Nurdin ◽  
Saiful Fakri
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Bark Extract ◽  
Test Results ◽  
Myristica Fragrans ◽  
Ic50 Value ◽  
Stem Bark Extract ◽  
Myristica Fragrans Houtt

Antioxidant activity test for the extract of ethyl acetate nutmeg stem bark (Myristica fragrans Houtt) was carried out using 2,2-diphenyl-1picrylhydrazil (DPPH). The test results of antioxidant activity of ethyl acetate nutmeg stem bark extract with DPPH method at a concentration of 25 ppm, 50 ppm and 100 ppm obtained IC50 value = 68.14 ppm with IC50 value of vitamin C (as positive control) 3.657 ppm. The results of column chromatographic separation of ethyl acetate nutmeg stem bark extract obtained 5 combined fractions namely MFEK 1, MFEK 2, MFEK 3, MFEK 4 and MFEK 5. The test results of the fraction antioxidant activity in a good category were the MFEK 1 fraction. The MFEK 2 fraction to MFEK 5 showed an antioxidant activity lower than compared  to the ethyl acetate extract. This shows that the ethyl acetate nutmeg stem bark extract has a better inhibitory activity, because there are several active compounds contained in the extract which can inhibit free radicals, so the IC50 value of the extract is better than the fractions.

scholarly journals TYROSINASE INHIBITION, 2,2-DIPHENYL-1-PICRYLHYDRAZYL RADICAL SCAVENGING ACTIVITY, AND PHYTOCHEMICAL SCREENING OF FRACTIONS AND ETHANOL EXTRACT FROM LEAVES AND STEM BARK OF MATOA (POMETIA PINNATA)

2017 ◽  
Vol 10 (17) ◽  
pp. 85
Author(s):  
Rani Sauriasari ◽  
Nur Azizah ◽  
Katrin Basah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Stem Bark ◽  
Scavenging Activity ◽  
Tyrosinase Inhibitor ◽  
Active Fraction

Objective: This study aims to investigate the potency of matoa as a tyrosinase inhibitor and antioxidant and also to identify the chemical compounds in the most active fraction and an ethanol extract from the leaves and stem bark of matoa.Methods: The extracts were tested for their tyrosinase inhibitory activity by evaluating the formation of L-dopachrome at 490 nm. Antioxidant activity was tested using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The most active extract from liquid-liquid partition analysis was fractionated, and then, the assays were performed.Results: The ethanol extract of leaves and stem bark of matoa showed weak anti-tyrosinase activity (percent inhibition was 24.54±0.22% and 21.93±0.57%, respectively, final concentration 200 μg/mL), but they showed strong DPPH radical scavenging activity (IC50 values were 6.11 μg/mL and 5.47 μg/mL, respectively). The ethyl acetate fraction was the most active fraction with an IC50 value of 5.38 μg/mL. Ethanol extract from the leaves and stem bark of matoa and the ethyl acetate fraction contains flavonoids, tannins, saponins, triterpenoids, and glycosides.Conclusion: Matoa does not have potency as a tyrosinase inhibitor, but it has good antioxidant activity, although still lower than that of quercetin.

scholarly journals FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK KULIT BATANG Chisocheton sp. (C.DC) Harms (Meliaceae)

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Dewa Gede Katja
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Bark Extract ◽  
Test Results ◽  
Ic50 Value ◽  
Dpph Method ◽  
Stem Bark Extract

Tujuan penelitian ini untuk mengetahui golongan senyawa metabolit sekunder dan aktivitas antioksidan dari setiap ekstrak kulit batang Chisocheton sp. C.DC Harms (Meliaceae). Hasil ekstraksi 200 g serbuk kulit batang Chisocheton sp. C.DC Harms (Meliaceae) dengan n-heksana, etil asetat dan metanol masing-masing dengan 2000 mL berturut-turut menghasilkan 7,193 g ekstrak pekat n-heksana, 8,798 g ekstrak pekat etil asetat dan 18,683 g ekstrak pekat metanol. Hasil uji fitokimia menunjukkan adanya golongan senyawa flavonoid, triterpenoid dan tanin. Hasil uji aktivitas antioksidan dengan metode DPPH menunjukkan bahwa ekstrak n-heksana memberikan nilai IC50 sebesar 337,28 µg/mL, ekstrak metanol sebesar 216,73 µg/mL, dan ekstrak etil asetat sebesar 199,89 µg/mL yang berarti etil asetat memiliki kemampuan yang paling besar dalam menangkap radikal bebas.ABSTRACTThe objective of this study was determine the class of secondary metabolite compounds and antioxidant activity of each stem bark extract of Chisocheton sp. C.DC Harms (Meliaceae). The results of the extraction of 200 g Chisocheton sp. C.DC Harms (Meliaceae) with n-hexane, ethyl acetate and methanol with 2000 mL each yielded 7,193 g of concentrated extract n-hexane, 8,798 g of concentrated extract of ethyl acetate and 18,683 g of concentrated methanol extract. Phytochemical test results showed the presence of flavonoid, triterpenoid and tannin compounds. The results of the antioxidant activity test using the DPPH method showed that the n-hexane extract gave an IC50 value of 337,28 µg/mL, methanol extract was 216,73 µg/mL, and ethyl acetate extract was 199,89 µg/mL which means that ethyl acetate has the ability to greatest in capturing free radicals.

scholarly journals ANTIOXIDANT ACTIVITY OF THE ASCIDIAN MARINE INVERTEBRATES, DIDEMNUM SP.

2018 ◽  
Vol 10 (1) ◽  
pp. 81
Author(s):  
Berna Elya ◽  
Yasman . ◽  
Zulfa Edawati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Marine Invertebrates ◽  
Ethyl Acetate Fraction ◽  
Dpph Radical ◽  
Active Fraction ◽  
Dpph Method ◽  
Water Fractions

Objective: This study aimed to determine the antioxidant activity of samples of the ascidian Didemnum sp. collected from Seribu Islands, Jakarta.Methods: Antioxidant activity was tested using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Didemnum sp. was extracted into methanol andthen fractionated using n-hexane, ethyl acetate, and water. Fractions with the highest antioxidant activity were further fractionated using acceleratedcolumn chromatography.Results: The concentration of sample that reduced the DPPH radical by 50% (IC50) in a methanol extract of Didemnum sp. was 105.10 μg/mL. The ethylacetate fraction had the highest antioxidant activity (IC50 of 90.804 μg/mL). The most active fraction obtained from accelerated column chromatographyfraction had an IC50 of 86.35 μg/mL. The compounds contained in the most active fractions were alkaloids, saponins, steroids/triterpenoids, andglycosides.Conclusion: The methanol extract of the ascidian Didemnum sp. exhibited antioxidant activity. Fractionation of the Didemnum sp. extract showed thatthe ethyl acetate fraction had the highest antioxidant activity. Further, fractionation of the ethyl acetate fraction by accelerated column chromatographyshowed that fraction VI had the highest antioxidant activity. The most active fraction contained alkaloids, steroids/triterpenoids, saponins, andglycosides.

Verbascum sinaiticum: A Rich Source of Antioxidant Phenylethanoid Glycosides

2020 ◽  
Vol 10 (2) ◽  
pp. 158-162 ◽  
Author(s):  
Humaira Yasmeen Gondal ◽  
Roshan Zamir ◽  
Muhammad Nisar ◽  
Muhammad Iqbal Choudhary
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Aerial Part ◽  
Methanolic Extract ◽  
Water Soluble ◽  
Antioxidant Potential ◽  
Active Fraction ◽  
Phenylethanoid Glycosides ◽  
Active Constituents ◽  
Soluble Part

Background: The genus Verbascum is well documented for its antioxidant potential but Verbascum sinaiticum is comparatively less studied plant. The current study was carried out to search for antioxidant nutraceuticals from this species. Objective: To explore the antioxidant potential of Verbascum sinaiticum and to identify its active constituents. Methods: The methanolic extract of air-dried aerial part of the Verbascum sinaiticum was partitioned with hexane, chloroform and ethyl acetate. The water-soluble part of ethyl acetate afforded six phenylethanoid glycosides by repeated chromatography over Sephadex LH-20, silica gel and ODS columns. Antioxidant activity of solvent extracts and isolated constituents were evaluated by DPPH, ABTS and FRAP assays. Results: Six phenylethanoid glycosides was isolated and characterized as Verbascoside, Eukovoside, Martynoside, Jionoside D, Campneoside I and Campneoside II, from the most active fraction. Conclusion: Verbascum sinaiticum demonstrated prospective antioxidant activity. The watersoluble part of EtOAc (WSEAE) was found the most active extract whereas Verbascoside was identified as the most potent constituent. All isolated compounds exhibited significant antioxidant activity whereas their synergistic effect was found prominent in the parent fraction.

scholarly journals Actividad antioxidante de extractos de hojas de Bocconia frutescens L. (Papaveraceae)

2016 ◽  
Vol 14 (2) ◽  
Author(s):  
Oscar Eduardo Rodríguez Aguirre ◽  
William Alejandro Andrade Barreiro ◽  
William Alejandro Andrade Barreiro ◽  
Fabio Eduardo Diaz Lopez ◽  
Fabio Eduardo Diaz Lopez
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Dpph Radical ◽  
Small Tree ◽  
High Antioxidant Activity ◽  
Para Determinar ◽  
Percent Uptake ◽  
Orange Color ◽  
Higher Sensitivity

<p>B<em>occonia frutescens </em>es un árbol pequeño, posee hojas profundamente lobuladas y con borde dentado, las flores están agrupadas en panículas terminales pendulares, las semillas son de color negro brillante con una cubierta carnosa de color anaranjada. Es nativa de América, es conocida con los nombres vulgares de: Sarno, Palo Amarillo o Trompeto. <strong>Objetivos: </strong>Evaluar la actividad antioxidante de extractos y fracciones de hojas de Bocconia frutescens por los métodos DPPH• y ABTS•+. <strong>Métodos: </strong>las fracciones obtenidas por soxleth con solventes de diferente polaridad de hojas, fueron evaluadas a concentraciones de 25, 62.5, 125 y 250 mg/LMeOH para determinar la actividad antioxidante por los métodos DPPH• y ABTS•+. <strong>Resultados: </strong>Para los extractos por el método decoloración del radical DPPH• el Porcentaje de Captación se encontró entre 47.6 y 57.7 a 250 mg/LMeOH y para las fracciones entre 45.4 y 54.1 mg/LMeOH, a 250 mg/LMeOH. Por el método decoloración del radical ABTS•+. Para los extractos el porcentaje de Captación se encontro entre 89.7 y 99.7 a 250 mg/LMeOH y para las fracciones entre 68.5 y 99.6 mg/LMeOH. <strong>Conclusiones: </strong>las fracciones de acetato de etilo y metanol presentaron una alta actividad antioxidante al ser evaluadas por las técnicas ABTS•+ y DPPH• , la técnica ABTS•+ se presento mayor sensibilidad.</p><p>Abstract</p><p>Bocconia frutescens is a small tree, has deeply lobed and serrated edge leaves, flowers are grouped in pendulous panicles terminal, seeds are shiny black with a fleshy covering of orange color. It is native to America, is known under their common names: Sarno, Palo Amarillo or Trompeto. Objectives: Evaluate the antioxidant activity of extracts and fractions of leaves Bocconia frutescens by DPPH• and ABTS•+ methods. Methods: the fractions obtained by Soxhlet with solvents of different polarity of leaves, were evaluated at concentrations of 25, 62.5, 125 and 250 mg / LMeOH to determine the antioxidant activity by DPPH• and ABTS•+ methods. Results: Extracts for the bleaching method DPPH• radical the Percent Uptake was between 47.6 and 57.7 to 250 mg / LMeOH and fractions between 45.4 and 54.1 mg / LMeOH, to 250 mg / LMeOH. By the method of coloration radical ABTS•+. Extracts for the percentage uptake was found between 89.7 and 99.7 and fractions between 68.5 and 99.6 mg / LMeOH, to 250 mg / LMeOH. Conclusions: The fractions of ethyl acetate and methanol have a high antioxidant activity to be evaluated by the ABTS•+ and DPPH• techniques, the technique ABTS•+ present higher sensitivity.</p>

scholarly journals Quick Preparative Separation of Natural Naphthopyranones with Antioxidant Activity by High-Speed Counter-Current Chromatography

2002 ◽  
Vol 57 (11-12) ◽  
pp. 1051-1055 ◽  
Author(s):  
Gilda G. Leitão ◽  
Suzana G. Leitão ◽  
Wagner Vilegas
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
High Speed ◽  
Ethanolic Extract ◽  
Solvent System ◽  
Spectrophotometric Assay ◽  
Preparative Separation ◽  
Dpph Radical ◽  
Preparative Scale ◽  
Counter Current

The natural naphthopyranones paepalantine (1), paepalantine-9O-β-ᴅ-glucopyranoside (2) and paepalantine-9-O-β-ᴅ-allopyranosyl-(1→6)-O-β-ᴅ-glucopyranoside (3) were separated in a preparative scale from the ethanolic extract of the capitula of Paepalanthus bromelioides by high-speed counter-current chromatography (HSCCC). The solvent system used was composed of water-ethanol-ethyl acetate-hexane (10:4 : 10:4, v/v/v/v). This technique led to the separation of the three different naphthopyranone glycosides in pure form in approximately 7 hours. Paepalantine showed a good antioxidant activity when assayed by the DPPH radical spectrophotometric assay.

scholarly journals Loranthus pulverulentus: A Potent Source of Natural Antioxidants and Alternative Medicine

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Muhammad Asam Raza ◽  
Rukhsana Kausar ◽  
Faraz Ali Rana ◽  
Muhammad Danish ◽  
Durre Shahwar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Natural Antioxidants ◽  
Stem Bark ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Strong Correlations ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Total Antioxidant

This study was designed to evaluate the antioxidant potential ofLoranthus pulverulentus. Stem bark, leaves, and seeds ofLoranthus pulverulentuswere extracted in methanol:water (90 : 10) and partitioned with n-hexane, chloroform, ethyl acetate, and n-butanol successively using partition chromatography. Total phenolic contents and antioxidant potential were checked using standard protocols. Total phenolic contents of all extracts were determined, using Folin–Ciocalteu reagent, and ranged between 151 ± 2.1 and 396 ± 1.6 for stem bark, 137 ± 0.9 and 430 ± 2.2 for, and 39 ± 0.6 and 231 ± 1.7 for seeds. The antioxidant potential of extracts was evaluated; namely, DPPH, FRAP, and total antioxidant models. The ethyl acetate extract of stem-bark, leaves, and seeds showed the highest activity in DPPH (94.5 ± 2.1%, 96.30 ± 0.9%, and 92.30 ± 1.1%, IC5015.9 ± 0.5 μg, 14.5 ± 0.8, and 102.7 ± 1.3, resp.), FRAP (7.7 ± 0.6, 7.5 ± 0.7 and 6.6 ± 0.7, resp.), and total antioxidant (0.95 ± 0.09, 1.19 ± 0.09, and 0.686 ± 0.08, resp.). Strong correlations were observed between total phenols versus total antioxidant activity, DPPH, and FRAP withR2values ranging from 0.8185 to 0.9951 (stem-bark), 0.6728 to 0.8648 (leaves), and 0.8658 to 0.9910 (seed) which indicated that phenolic contents are the major constituents responsible for antioxidant activity.

scholarly journals Chromatographic Analysis and Antioxidant Capacity of Tabernaemontana catharinensis

2014 ◽  
Vol 9 (1) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Aline A. Boligon ◽  
Mariana Piana ◽  
Thiago G. Schawnz ◽  
Romaiana P. Pereira ◽  
João B. T. Rocha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Crude Extract ◽  
Chromatographic Analysis ◽  
Thiobarbituric Acid ◽  
Stem Bark ◽  
Total Phenols ◽  
Dpph Assay ◽  
Effective Agent

In this study we evaluated the composition of the crude extract and fractions of Tabernaemontana catharinensis (Apocynaceae) by HPLC/DAD and GC/MS. We also tested the antioxidant capacity and investigated the contents of polyphenols, flavonoids, tannins and alkaloids of T. catharinensis stem bark. The extract and fractions showed inhibition against thiobarbituric acid reactive species (TBARS), in the following order: ethyl acetate (IC50 = 4.7 ± 0.2 μg/mL) > dichloromethane (23.9 ± 1.1 μg/mL) > n-butanolic (25.2 ± 0.4 μg/mL) > crude extract (38.0 ± 0.07 μg/mL). Moreover, the DPPH assay, presented IC50 values ranged from 5.6 ± 0.6 to 30.3 ± 1.3 μg/mL. Contents of total phenols, flavonoids, tannins and alkaloids of T. catharinensis followed the order: ethyl acetate > n-butanolic > dichloromethane fractions > crude extract. HPLC/DAD analyses indicated that gallic, chlorogenic and caffeic acids, and rutin, quercetin and kaempferol are components of the species. Taken together, the results suggest that T. catharinensis could be considered an effective agent in the prevention of diseases associated with oxidative stress.

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