CYP2E1 Polymorphisms and Colorectal Cancer

Background: The hepatic cytochrome P450 2E1 (CYP2E1) enzyme plays an important role in the metabolic activation of nitrosamines and other carcinogenic compounds. Aim of the study: To determine the effect of chemotherapy and radiation for colorectal cancer patients on some biochemical and molecular parameters. Patients and methods: The current study was conducted in the Department of Biology at the College of Science at the University of Al-Qadisiyah and in cooperation with the Cancer Center in the Najaf city and the Department of Medicine City in Baghdad, Iraq during the period from November 15, 2019, to May 6, 2020. The study group included a total of (90) samples were taken from males and females of ages ranged between (33 years to 80 years) and then it was divided into two groups. The first included (60) samples of people with colorectal cancer (CRC), and the second included (30) samples of people The healthy ones represented the control group. (5ml) of venous blood was withdrawn and the sample was divided into two groups, the first was placed in tubes containing an anti-clotting substance for molecular examination and kept at a temperature (-20) until use, and the other part was placed in Gel tube tubes and then placed in a centrifuge to separate Serum, for the purpose of biochemical examinations. With regard to the results of the values of oxidative stress indicators (SOD, GSH, MDA). Results: there was a significant difference in the level of (SOD, GSH) between the control and treatment groups, where the highest percentage was in the control group, followed by the radiotherapy group and then chemotherapy. The results showed a significant increase in the MDA value of the chemotherapy group compared with the radiotherapy and control group. The results of the molecular study to study the genotypes of the CYPIE2 gene showed that the C1/C1 type is the normal type for the CYPIE2 gene and that the ratio of this type did not show any significant difference between the groups, while the C2/C2 type showed no significant difference between the groups and the C2/C2 type. Conclusion: It can be concluded that the use of radiotherapy and chemotherapy to treat patients with CRC cancer has a clear effect on health and on many of the patient's physiological and biochemical indicators, and this, in turn, is reflected in the patient's ability to fight the disease.

The Effect of Balloon Inflating Distraction on Pain Intensity During Venous Blood Sampling in Children: A Randomized Controlled Trial

10.21203/rs.3.rs-711305/v1 ◽

2021 ◽

Author(s):

Somaeh. Salehi Sarbijan ◽

Roghiah Mehdipoor Rabori ◽

Esmat Nouhi

Keyword(s):

Venous Blood ◽

Blood Sampling ◽

Control Group ◽

Negative Consequences ◽

Randomized Controlled ◽

Pain Scores ◽

Significant Difference ◽

Average Pain ◽

And Control ◽

Venous Blood Sampling

Abstract Background: A large number of children are subject to prevention, diagnosis, and treatment procedures that mostly require venous blood sampling. Painful procedures such as blood sampling in children have annoying physical and emotional effects and can lead to wider negative consequences such as physical, behavioral, and social disorders in children. This study aimed to determine the effect of distraction by balloon inflating on the amount of pain during taking blood samples from children aged 4 to 7. Method: This study had a randomized controlled clinical trial that was conducted on 80 children admitted to the pediatric department of Imam Khomeini Hospital in Jiroft-Kerman province who met the inclusion criteria after obtaining their parent’s consent. The children were randomly grouped into the intervention (n = 40) and control (n = 40) groups. The pain level in children in the intervention group was measured during the venous blood sampling while the children were watching inflating a balloon. However, the pain in children in the control group was measured without any intervention during blood sampling. Wong–Baker Faces Pain Rating Scale questionnaire was used for pain assessment. Results: The average pain scores for the children in the two groups showed no significant difference before the intervention but the average pain scores after the intervention in the intervention and control groups were 1.38±2.56 and 4.2±1.58; showing a significant difference between the two groups after the intervention (p-value < 0.05). Conclusion: Using balloon inflation distraction during venous blood sampling reduces pain in children.

Effectiveness of ColorApp Mobile Application for Health Education and Promotion on Colorectal Cancer: A Quasi-Experimental Study (Preprint)

10.2196/preprints.15487 ◽

2019 ◽

Cited By ~ 1

Author(s):

Nor Azwany Yaacob ◽

Muhamad Fadhil Mohamad Marzuki ◽

Najib Majdi Yaacob ◽

Shahrul Bariyah Ahmad ◽

Muhammad Radzi Abu Hassan

Keyword(s):

Colorectal Cancer ◽

Mobile Application ◽

Intervention Group ◽

Control Group ◽

The Public ◽

Screening Participation ◽

Significant Difference ◽

Quasi Experimental ◽

Knowledge And Attitude ◽

BACKGROUND Background: Lack of knowledge and poor attitude is one of the barriers to colorectal cancer screening participation. Printed material such as pamphlets and posters were the main approach in health education on disease prevention. Current information technology advancement had seen an increasing trend of the public to read from websites and mobile app using their smartphone. Thus, health information dissemination should also be diverted to the current trend of information search. Increase in knowledge and awareness will hopefully increase the screening participation and prevent late detection of diseases such as colorectal cancer. OBJECTIVE This study aimed to assess the effectiveness of ColorApp mobile applications in improving the knowledge and attitude on colorectal cancer among user aged 50 years old and above who are the population at risk for the disease in Kedah. METHODS Methods: A quasi-experimental study has been conducted involving 100 participants in Kedah, Malaysia. Participants from five randomly selected community empowerment program in Kota Setar district was chosen as the intervention group while Kuala Muda district was chosen as the control group. Participants were given a self-administered validated questionnaire on knowledge and attitude towards colorectal cancer. A usable mobile application, the ColorApp (Colorectal Cancer Application) was developed as a new educational tool on colorectal cancer prevention. The intervention group were given 2 weeks to use the ColorApp. The same questionnaire was redistributed to both groups after two weeks. The mean percentage score for knowledge and attitude between groups were compared using Repeated Measure ANCOVA. RESULTS Result: There was no significant difference of age, sex, highest education level, current occupation, and diabetic status between the two groups. The number of smokers was significantly higher in intervention groups as compared to control group and it has been controlled during analysis. There was a significant difference in mean knowledge score between intervention and control group with regards to time [Huynh Feldt: F (1,95) = 19.81; p < 0.001]. However, there was no significant difference in mean attitude score between intervention and control group with regards to time [F (1,95) = 0.36, p = 0.550]. CONCLUSIONS Conclusion: The mobile application can be one of the adjunct approaches in educating the public on colorectal cancer.

The Effect of Education Provided to Family Members Caring for Colorectal Cancer Patients on Caregiving Reactions and Healthy Lifestyle Behaviors: A Prospective Quasi-Experimental Study

10.21203/rs.3.rs-865291/v1 ◽

2021 ◽

Author(s):

nilay bektas akpınar ◽

Tulin Beduk ◽

Filiz Cay Senler

Keyword(s):

Colorectal Cancer ◽

Healthy Lifestyle ◽

Control Group ◽

Lifestyle Behaviors ◽

Healthy Lifestyle Behaviors ◽

Post Test ◽

Quasi Experimental ◽

And Control ◽

Experimental Group

Abstract Purpose: To evaluate the effects of the educational package provided to enhance family caregivers' experience of colorectal cancer patients receiving chemotherapy on healthy lifestyle and caregiving reactions.Methods: The study was conducted as a pre-test-post-test, quasi-experimental intervention with a control group. The study population consisted of 100 caregivers who provide primary care to patients with colorectal cancer. The data were collected using the "Socio-Demographic Characteristics Data Collection Form", the "Healthy Lifestyle Behaviors Scale-II", and the "Caregiver Reaction Assessment” forms. The pre-test was applied to the experimental and control groups at the first interview. After the preliminary interview, the experimental group was applied three times to face-to-face individual educational sessions through the education booklet prepared by taking the opinions of 5 academician nurses who are experts in the oncology field. The post-test then was applied to the caregivers in the experimental and control groups.Results: After the education provided to the experimental group, a positive change was observed in the mean scores of all sub-dimensions of Healthy Lifestyle Behaviors Scale-II except for the physical sub-dimension. In the control group, there was a statistically significant negative change in the post-test mean scores in all sub-dimensions of the Caregiver Reaction Assessment Scale. Lack of family support in caregivers negatively affected interpersonal relationships, nutrition, health responsibility, and spiritual growth.Conclusion: In order to reduce the problems in family members, it is important to interact with a multidisciplinary approach not only with the patient but also with the caregivers during the chemotherapy process. Clinical Trials Identifier; NCT04791982, 10.03.2021, retrospectively registered

The comparison of total capacity antioxidant in the serum of people with pterygium and control subjects

International Eye Research ◽

10.18240/ier.2020.01.02 ◽

2020 ◽

Vol 1 (1) ◽

pp. 7-12

Author(s):

Heydari Behrooz ◽

◽

Zarban Asghar ◽

Hosseini Rad Abbas ◽

Feizmohammadi Akram ◽

...

Keyword(s):

Antioxidant Capacity ◽

Clinical Symptoms ◽

Venous Blood ◽

Power Measurement ◽

Control Group ◽

Antioxidant Power ◽

Control Subjects ◽

Significant Difference ◽

The Mean ◽

AIM: To investigate the comparison of total antioxidant capacity in the serum of patients with pterygium and control subjects. METHODS: This case-control study was conducted on all persons referred to Ophthalmology Clinic of teaching Hospital of Vali-Asr (peace upon to him) with clinical symptoms of pterygium during the year 2016. The control group was selected among patients referred to the Ophthalmology Clinic of Vali-Asr (peace without pterygium) that the two groups were matched in terms of age, gender and place of residence. Sixty-six persons [31 people (47%) in patient group and 35 people (53%) in the control group] were enrolled by convenience sampling. Venous blood sample was taken from all patients after the sampling using ferric reducing antioxidant power (FRAP); FRAP- as a quick 10min measurement, the antioxidant power measurement of samples according to the conversion of ferric iron (Fe3+) to ferrous iron (Fe2+) was checked. The collected data ware entered to software SPSS 21 and were analyzed by chi-square and Mann-Whitney tests at the level of α =0.05. RESULTS: The mean of antioxidant capacity in patients was 842/55±161/46 μmol/L and antioxidant capacity in healthy controls was 856/77±209/41 μmol/L (P=0.8). In the comparison of mean serum antioxidant capacity in healthy individuals and in the serum of people with pterygium based on gender the results showed that the antioxidant capacity mean in male control subjects has been 894/05± 176/82 μmol/L and in females control 780/01±118/33 μmol/L that the observed difference have been reported statistically significant (P=0.008) but the other comparison according the gender between cases and control does not show any significant difference. CONCLUSION: The results of this study showed that the full level of serum antioxidant capacity in patients has been less than the mean of antioxidant capacity in control subjects; however, the observed difference has not been significant. The results of this study were consistent with basic results carried out on the damaging effects of oxidative stress in the pterygium pathogenesis. Recommending diet with minerals and vitamins containing antioxidants may be preventing the onset and progression of pterygium.

Adamalysines as Biomarkers and a Potential Target of Therapy in Colorectal Cancer Patients: Preliminary Results

Disease Markers ◽

10.1155/2019/5035234 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Katarzyna Walkiewicz ◽

Joanna Strzelczyk ◽

Dariusz Waniczek ◽

Krzysztof Biernacki ◽

Małgorzata Muc-Wierzgoń ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Patients ◽

Distant Metastases ◽

Serum Levels ◽

Control Group ◽

And Control ◽

The Relationship ◽

Increased Activity

Colorectal cancer is one of the most common cancers in the world. Due to its still undetermined pathogenesis, we are searching for signaling pathways that are important in the development of colorectal cancer. In this article, we present results of study on the role of ADAM proteins in colorectal cancer. The study included 85 adult colorectal cancer patients (48 men, 37 women) and 25 patients in the control group (after diagnostic colonoscopy—without cancer). During hospitalization, a serum sample (3 cm3) was collected from the study and control group, anthropometric measurements were conducted and others clinical data were analyzed. In the serum ADAM10, 12, 17, and 28, protein concentrations were determined and, in the next step, examined the relationship between ADAMs concentrations and selected clinical parameters in both groups. The analysis showed that serum levels of ADAM10 and ADAM28 are significantly higher in patients with colorectal cancer and correlate with histopathological grading and with presence of distant metastases. Moreover, noticed the trend to correlate concentrations of adamalysines with higher BMI score. One of the functions of adamalysines is the activation of growth factors involved in cancer, including IGF and TNFα. The increased activity of adamalysines in patients may play a role in the pathogenesis of colorectal cancer. Our study highlights the prevalence of metabolic disorders in the group of patients with diagnosed CRC, and this cancer seems to be a further complication of obesity.

Genetic polymorphisms affecting antioxidant enzymes are present in tympanosclerosis patients

The Journal of Laryngology & Otology ◽

10.1017/s0022215116008732 ◽

2016 ◽

Vol 130 (10) ◽

pp. 928-933 ◽

Cited By ~ 1

Author(s):

A Akyigit ◽

Ş Yalcin ◽

E Ö Etem ◽

İ Kaygusuz ◽

T Karlidag ◽

...

Keyword(s):

Genetic Polymorphisms ◽

Otitis Media ◽

Venous Blood ◽

Chronic Otitis Media ◽

Study Groups ◽

Control Group ◽

Genotype Distribution ◽

Control Groups ◽

Significant Difference ◽

AbstractBackground:This study investigated genetic polymorphisms affecting the inducible nitric oxide synthase, superoxide dismutase and catalase enzymes in chronic otitis media patients with and without tympanosclerosis, and the role of genetic susceptibility in the disease aetiology.Methods:A total of 162 patients who underwent surgery for chronic otitis media were divided into two study groups: a tympanosclerosis group and a chronic otitis media group. A third, the control, group comprised 188 healthy volunteers. Venous blood samples were evaluated using reverse transcriptase polymerase chain reaction.Results:There was a significant difference in GG genotype distribution of the −277A>G polymorphism in the NOS2 gene between the tympanosclerosis and control groups (p < 0.05). However, there were no significant between-group differences in the CC genotype distribution of the p.Ala16Val (C>T) polymorphism in the SOD2 gene (p > 0.05). There were significant differences in the TT genotype distribution of the −21A>T polymorphism in the CAT gene between the tympanosclerosis and control groups, and between the chronic otitis media and control groups (p < 0.05).Conclusion:These results suggest that genetic predisposition may play a role in the aetiopathogenesis of tympanosclerosis.

Studying the Frequency of Autoimmune Thyroid Diseases in Duhok Province

Science Journal of University of Zakho ◽

10.25271/sjuoz.2019.7.2.587 ◽

2019 ◽

Vol 7 (2) ◽

Author(s):

Rozheen I. Hasan ◽

Amir H. Raziq

Keyword(s):

Venous Blood ◽

Serum Levels ◽

Control Group ◽

Thyroid Disorder ◽

Control Groups ◽

Autoimmune Thyroid Diseases ◽

Autoimmune Thyroid ◽

Significant Difference ◽

The One ◽

Thyroid disorders are one of the most frequent disorders affecting endocrine system. The present study aimed to identify the frequency of thyroid disorder in our locality at Duhok city. To do so, a total of one hundred patients and one hundred apparently healthy control individuals were enrolled in this study. Samples of venous blood (5 ml) were withdrawn from patients and control subjects and analysed. Clinical assessment was performed by a specialist and each patient provided with a document supporting preliminary clinical diagnosis. Measurements of serum TSH, T3, T4, and anti-thyroperoxidase antibodies (anti-TPO antibodies) serum levels were performed. All assays were conducted at Duhok central health laboratory. Out of the one hundred patients and the one hundred control groups, forty-two and thirty candidates were randomly selected, respectively. The mean TSH serum levels of 25.87 (uIU/ml) for the patients significantly differ from that of the control group 3.55 (uIU/ml) (p<0.001). However, T3 and T4 serum levels indicated no significant difference between the two categories. Moreover, there was statisticaly significant difference (p< 0.0001) between the patients and control groups when their mean serum anti-TPO concentrations were compared. In conclusion, considerable number of subjects appeared to be candidates for deranged thyroid functions and is susceptible to autoimmune thyroid disorder.

Study on the Changes of Serum Vaspin Level and its Regulatory Mechanism in Patients with Thyroid Dysfunction

Journal of Clinical and Nursing Research ◽

10.26689/jcnr.v4i6.1609 ◽

2020 ◽

Vol 4 (6) ◽

Author(s):

Zhenzuo Li ◽

Jian Zhang ◽

Lin Li ◽

Xiaoxia Pan ◽

Li Zhang ◽

...

Keyword(s):

Thyroid Dysfunction ◽

Venous Blood ◽

Ankle Brachial Index ◽

Resistance Index ◽

Blood Lipid ◽

The Body ◽

Control Group ◽

Model Assessment ◽

Significant Difference ◽

Objective: To study the changes of serum vaspin levels in hyperthyroidism and hypothyroidism, and the correlation between serum vaspin and FT3, FT4, TSH and HOMA-IR. Methods: According to the diagnostic criteria of hyperthyroidism and hypothyroidism published in the 8th edition of internal medicine, the patients were divided into hyperthyroidism group (n = 47), male 14, female 33, average age (35 ± 9) years; hypothyroidism group: 23 hypothyroidism patients, 7 males and 16 females, with an average age of (38 ± 10) years. The blood pressure, height and weight of all the participants were measured by a specially assigned person, and the body mass index (BMI = weight (kg) / height (M2) and ankle brachial index (ABI) were calculated. Venous blood samples were drawn from all subjects after fasting for 8 hours in the morning to determine biochemical indexes. Fasting insulin (fins) was measured by chemiluminescence method, insulin resistance index (HOMA-IR, HOMA-IR = FPG × fins / 22.5) was calculated by homeostasis model assessment (HOMA-IR), and HbA1c was determined by high-pressure liquid chromatography. The levels of FT3, FT4 and TSH were detected by radioimmunoassay. Serum vaspin levels were measured by ELISA. Results: The level of BMI in hypothyroidism group was significantly higher than that in hyperthyroidism group and control group (P < 0.01), BMI level in hyperthyroidism group was significantly lower than that in control group (P < 0.05), FT3 and FT4 levels in hyperthyroidism group were significantly higher than those in hypothyroidism group and control group (P < 0.01), TSH level in hypothyroidism group was significantly higher than that in control group and hyperthyroidism group (P < 0.01). The level of FPG in hyperthyroidism group was significantly higher than that in control group (P < 0.01), but there was no significant difference between hyperthyroidism group and hypothyroidism group, and fins level in hypothyroidism group was significantly higher than that in control group and hyperthyroidism group (P < 0.01). The level of HOMA-IR in hyperthyroidism and hypothyroidism group was significantly higher than that in control group (P < 0.01). Compared with the control group and the control group, the blood lipid indexes (TC, LDL-C) in the hyperthyroidism group were lower than those in the control group and hypothyroidism group (P<0.01), and all the blood lipid indexes in the hypothyroidism group were significantly different from those in the control group (P<0.01). The vaspin level of hyperthyroidism group was significantly higher than that of control group and hypothyroidism group, and the latter two groups showed that the level of vaspin in hypothyroidism group was significantly lower than that of control group (P < 0.05). Correlation analysis showed that serum vaspin was positively correlated with FT3 and FT4 (r = 0.255, P = 0.005; r = 0.327, P = 0.001), and negatively correlated with BMI, TC and HDL (r = -0.250, P = 0.006; r = -0.244, P = 0.007; r = -0.258, P = 0.004). ) Conclusion: Serum vaspin level is related to thyroid function. The level of serum vaspin increases in hyperthyroidism and decreases in hypothyroidism. Abnormal changes of fat factor vaspin are associated with thyroid dysfunction.

Safety of intraoperative chemotherapy with 5-FU for colorectal cancer patients receiving curative resection: A randomized, multicenter, prospective, phase III IOCCRC trial (IOCCRC).

Journal of Clinical Oncology ◽

10.1200/jco.2017.35.15_suppl.e15002 ◽

2017 ◽

Vol 35 (15_suppl) ◽

pp. e15002-e15002

Author(s):

Rong-xin Zhang ◽

Jun-zhong Lin ◽

Gong Chen ◽

Li-ren LI ◽

Zhen-hai LU ◽

...

Keyword(s):

Colorectal Cancer ◽

Side Effects ◽

Cancer Patients ◽

Surgical Resection ◽

Control Group ◽

Control Groups ◽

Safety And Efficacy ◽

And Control ◽

Intraoperative Chemotherapy

e15002 Background: The safety and efficacy of intraoperative chemotherapy in colorectal cancer have not yet been extensively investigated. This randomized control trial was designed to compare the safety and efficacy of intraoperative chemotherapy in combination with surgical resection to those of traditional surgical resection alone. Patients and Methods: From January 2011 to January 2016, 696 colorectal cancer patients were enrolled in this study: 341 patients were randomly assigned to the intraoperative plus surgery group to receive intraoperative chemotherapy (portal vein, intraluminal, and intraperitoneal chemotherapy plus surgical resection), whereas 344 patients were randomized to the control group to undergo surgery alone. Eleven patients withdrew consent. Surgical complications and side effects of intraoperative chemotherapy were compared between the two groups. Results: Intraoperative chemotherapy did not increase the rate of surgical complications, and no severe chemotherapy-associated side effects were observed. There were no significant differences in the length of hospital stay (8.31 vs. 7.98 days, P = 0.138), length of surgery (170.4 vs. 167.0 min, P = 0.526), length of antibiotic use (2.28 vs. 2.15 days, P = 0.322), or length of drain usage (6.37 vs. 6.16 days, P = 0.387) between the intraoperative chemotherapy and control groups. Four patients in each of the intraoperative chemotherapy and the control groups experienced anastomotic leakage and underwent a second operation (1.2% vs. 1.2%, P = 0.99). There were no deaths within 90 days after surgery in the chemotherapy group, whereas one patient died in the control group (0% vs. 0.3%, P = 0.99). Intraoperative chemotherapy did not decrease the rate of patients who received postoperative adjuvant chemotherapy between the intraoperative group and control group (29.3% vs. 30.2%, P = 0.795). Conclusions: Intraoperative chemotherapy can be safely performed during colorectal surgery; however, follow-up is necessary for a better assessment of its efficacy. Register Number: NCT01465451. Clinical trial information: NCT01465451.

Pharmacokinetics-based Dose Management of 5-Fluorouracil Clinical Research in Advanced Colorectal Cancer Treatment

Mini-Reviews in Medicinal Chemistry ◽

10.2174/1389557519666191011154923 ◽

2020 ◽

Vol 20 (2) ◽

pp. 161-167

Author(s):

Rong Deng ◽

Lin Shi ◽

Wei Zhu ◽

Mei Wang ◽

Xin Guan ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Treatment ◽

Cancer Patients ◽

Advanced Colorectal Cancer ◽

Control Group ◽

Significant Difference ◽

Dose Management ◽

Experimental Group ◽

Colorectal Cancer Treatment

Objective: The study aimed to explore the efficacy of pharmacokinetic-based 5-fluorouracil dose management by plasma concentration test in advanced colorectal cancer treatment. Methods: 153 samples of advanced colorectal cancer patients were enrolled and randomly assigned to a control group and an experimental group. All patients received double-week chemotherapy with 5- fluorouracil (four weeks were used as one period), and chemotherapy duration ranged from 2 to 6 periods. In the first period, all patients were administered with the classic strategy of body surface area (BSA). Results: In the subsequent periods, the control group (77 samples) continued with BSA guided chemotherapy, while the experimental group (76 samples) received pharmacokinetic AUC-based chemotherapy. The efficacy and toxic side effects were assessed during chemotherapy, and survival was recorded in a follow-up. In the AUC experimental group, the rate of diarrhea significantly decreased (37.50% vs. 70.00%, P=0.010), and incidence of oral mucositis reduced (54.17% vs. 82.50%, P=0.014). Compared with the control group, the clinical benefit rate of experimental group was much higher (90.79% vs. 79.22%, P=0.046). Conclusion: There was no significant difference in other 5-fluorouracil related toxic side effect events (nausea, vomiting, hand-foot syndrome) and progression-free survival between the two groups. Pharmacokinetic- based dose management of 5-Fluorouracil reduces the toxicity of chemotherapy and improves long-term efficacy of chemotherapy for advanced colorectal cancer patients.