Antibacterial activity of the crude ethanolic extracts of Etlingera elatior and Etlingera philippinensis

2020 ◽  
Vol 24 (2) ◽  
Author(s):  
Gina Barbosa ◽  
◽  
Cresilda Alinapon ◽  
Analyn Gultiano

Antibacterial, Etlingera elatior, Etlingera philippinensis


Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 624
Author(s):  
Laima Česonienė ◽  
Juozas Labokas ◽  
Ina Jasutienė ◽  
Antanas Šarkinas ◽  
Vilma Kaškonienė ◽  
...  

The aim of the study was to evaluate 11 cultivars of blue honeysuckle (Lonicera caerulea L.) for bioactive compounds, antioxidant capacity, and the antibacterial activity of berries. Total phenolic contents (TPCs) and total anthocyanin contents (TACs) were established by using ethanolic extracts. For contents of organic acids and saccharides, aqueous extracts were used, and vitamin C was determined by using oxalic acid solution. DPPH• radical scavenging capacity was evaluated by using ethanolic extracts; antibacterial activity was assessed by using both ethanolic and aqueous extracts. The TPC varied from 364.02 ± 0.41 mg/100 g in ‘Vostorg’ to 784.5 ± 0.3 mg/100 g in ‘Obilnaja’, and TAC ranged from 277.8 ± 1.1 mg/100 g in ‘Čelnočnaja’ to 394.1 ± 8.4 mg/100 g in ‘Nimfa’. Anthocyanins comprised 53.8% of total phenolic contents on average. Among organic acids, citric acid was predominant, averaging 769.41 ± 5.34 mg/100 g, with malic and quinic acids amounting to 289.90 ± 2.64 and 45.00 ± 0.37 mg/100 g on average, respectively. Contents of vitamin C were 34.26 ± 0.25 mg/100 g on average. Organic acids were most effective in the inhibition of both Gram-positive and Gram-negative bacteria tested. In conclusion, berries of L. caerulea are beneficial not only for fresh consumption, but also as a raw material or ingredients of foods with high health-promoting value.



2019 ◽  
Vol 2 (1) ◽  
pp. 01-06
Author(s):  
Rhazi Fouzia

Study contextual: Faced with the global problem of antimicrobial resistance, and the use of traditional medicine for the research of antibacterial biomolecules. Aim: our work focused on the valorization of a medicinal plant Dittrichia viscosa which has many therapeutic and culinary virtues worldwide. Methods: To do this, a phytochemical screening of the leafy stems of the plant is carried out according to a set of physicochemical reactions, as well as an in vitro evaluation of the antibacterial activity, of the aqueous and ethanolic extracts against multi-resistant bacterial strains, by microdilution technique on microplates. An evaluation of the synergistic interaction between extracts and weakened antibiotics against pathogenic bacteria was also highlighted in this study. Results: The tests revealed the richness of Dittrichia viscosa species by tannins, flavonoids, saponosides, sterols and triterpenes. As for the antibacterial effect, the MICs range from 0.858±0.29 to 66.66 ± 0.00 mg / ml and the MBCs from 4.300 ± 1.01 to 11.610 ± 2.31 mg / ml is an interesting antibacterial activity. Regarding the combination of extracts with antibiotics tested, it revealed a synergistic action inducing an amplification of the antibacterial power of Penicillin, Imipenem and Erythromycin with a rate that reaches 471%. Conclusion: The results of this study show that the aqueous and ethanolic extracts of Dittrichia viscosa have interesting and promising antibacterial activity in the pharmaceutical, food and cosmetic industries.





F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1406 ◽  
Author(s):  
Ovin Qonita Allyn ◽  
Eko Kusumawati ◽  
Rudy Agung Nugroho

The aim of this study was to determine the effects of various concentration ofTerminalia catappabrown leaves extract which can inhibit the growth ofStaphylococcus aureusATCC 25923 andPseudomonas aeruginosaATCC 27853. The crushed-brown leaves ofTerminalia catappawas extracted using 95% ethanol, filtered, and evaporated. The driedT. catappaextract was used to identify phytochemical content qualitatively. Total phenolic and flavonoid contents were also measured quantitatively from dried extract. The dried extracts were also dissolved in sterile aquadest and serial dilutions were prepared to final concentration of 30, 60 and 90%. A disc diffusion method was used to evaluate the antibacterial activity of various concentrations of ethanol extract of brown leaves ofT. catappa. Inhibition zone diameter was measured to determine antibacterial activity. Gentamycin sulfate and distilled water were used as positive and negative controls, respectively. Dried ethanolic extract of brownT. catappaleaves contained flavonoid, quinon, phenolic, triterpenoid, and tannin. A total of 208.722 mg gallic acid equivalent/g extract of total phenolic and 35.7671 mg quercetin equivalent/g extract of total flavonoid were also found in the dried extract. The inhibition zone diameters of ethanolic extracts ranged from 1.73 to 9.06 mm (S. aureus) and from 1.83 to 6.5 mm (P. aeruginosa). The higher concentration of extract, the wider the inhibition zone diameters for both bacteria.P. aeruginosawas more resistant to high concentrations of extract (90%) thanS. aureus. Ethanolic extracts of the brown leaves ofT. catappahad different antibacterial effects againstS. aureusandP. aeruginosa. The higher the concentration of extract, the wider the inhibition zone diameter for both bacteria.P. aeruginosawas more resistant to high concentrations of ethanolic extracts of the brown leaves ofT. catappa.



2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Bishnu P. Marasini ◽  
Pankaj Baral ◽  
Pratibha Aryal ◽  
Kashi R. Ghimire ◽  
Sanjiv Neupane ◽  
...  

The worldwide increase of multidrug resistance in both community- and health-care associated bacterial infections has impaired the current antimicrobial therapy, warranting the search for other alternatives. We aimed to find thein vitroantibacterial activity of ethanolic extracts of 16 different traditionally used medicinal plants of Nepal against 13 clinical and 2 reference bacterial species using microbroth dilution method. The evaluated plants species were found to exert a range ofin vitrogrowth inhibitory action against the tested bacterial species, andCynodon dactylonwas found to exhibit moderate inhibitory action against 13 bacterial species including methicillin-resistantStaphylococcus aureus, imipenem-resistantPseudomonas aeruginosa, multidrug-resistantSalmonella typhi, andS. typhimurium. The minimum inhibitory concentration (MIC) values of tested ethanolic extracts were found from 31 to >25,000 μg/mL. Notably, ethanolic extracts ofCinnamomum camphora, Curculigo orchioides, andCurcuma longaexhibited the highest antibacterial activity againstS. pyogeneswith a MIC of 49, 49, and 195 μg/mL, respectively; whereas chloroform fraction ofCynodon dactylonexhibited best antibacterial activity againstS. aureuswith a MIC of 31 μg/mL. Among all,C. dactylon, C. camphora, C. orchioides, andC. longaplant extracts displayed a potential antibacterial activity of MIC < 100 μg/mL.



2020 ◽  
Vol 367 (16) ◽  
Author(s):  
Tabitha Mlowoka Kayira ◽  
Hiroyuki Nakano

ABSTRACT Vibrio cholerae is an etiological cause of cholera implicated in several pandemics. Antibacterial activity of plant extracts has been established. However, these extracts exhibit activity at a concentration that may alter organoleptic attributes of water and food, hence limiting their application. In this light, there is need to device ways of reducing plant extracts' effective levels in order to widen their application. Thus, this study was conducted to improve activities of plant ethanolic extracts through combination with other generally recognized as safe antimicrobials. Combination of plant extracts with sodium acetate (NaOAc) 0.4% at pH 7.0 reduced minimum inhibitory concentrations (MICs) of clove, lemon eucalyptus, rosemary and sage from 0.2 to 0.025%. At pH 6.4, combinations were more effective reducing MICs of clove, lemon eucalyptus, rosemary and sage from 0.2 to 0.0125% with NaOAc at 0.2%. At pH 7.0, the combination resulted in additive effect. Nevertheless, at pH 6.4, synergic effect was established. No interactive effect was observed with combinations involving glycine. Combination of plant extracts with NaOAc at mildly acidic pH creates a hurdle effect that may have potential application to control the growth of V. cholerae.



2017 ◽  
Vol 6 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Shakil Ahmed Polash ◽  
Tanushree Saha ◽  
Md Sharif Hossain ◽  
Satya Ranjan Sarker

Abstract not availableJahangirnagar University J. Biol. Sci. 6(1): 51-57, 2017 (June)



2011 ◽  
Vol 1 (1) ◽  
pp. S76-S78 ◽  
Author(s):  
Joy Jeba Malar T Renisheya ◽  
M Johnson ◽  
Uthith M Mary ◽  
A Arthy


2020 ◽  
Vol 7 (2) ◽  
pp. 51-58
Author(s):  
Rinda Binugraheni ◽  
Ndaru Trisni Larasati

Infectious diseases that often occur in humans are skin infections. One of the bacteria that cause infection in humans is Staphylococcus aureus. One of the plants used as an ingredient in traditional medicine and used as an anti-bacterial is a kecombrang plant. This research aims to test the antibacterial activity of leaf extract of kecombrang against Staphylococcus aureus. Kecombrang leaf obtained from Pandeglang Banten. Kecombrang leaves extracted with a maceration method using 96% ethanol solvent. Extracts of leaves are made in a various concentrations (100%, 75%, 50%, and 25%). The antibacterial activity test was performed by using the diffusion method to find out the large zone of diameter are formed to inhibiting Staphylococcus aureus bacteria.The results of the antibacterial activity of the leaves extract of kecombrang to Staphylococcus aureus at 25%, 50%, 75% and 100% concentrations respectively were 12.67 mm, 14.33 mm, 15.33 mm, and 17.00 mm. The data result showed, that leaf extract of kecombrang with 100% concentration had the largest inhibiting zone diameter of 17.00 mm, among other concentrations. This suggests that kecombrang leaf extract have antibacterial activity against Staphylococcus aureus.



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