scholarly journals PENGARUH PEMBERIAN EKSTRAK ETANOL RIMPANG LENGKUAS (ALPINIA GALANGA) TERHADAP GAMBARAN HISTOPATOLOGI HEPAR MENCIT (MUS MUSCULUS L.) YANG DIINDUKSI MONOSODIUM GLUTAMATE

2020 ◽  
Vol 7 (2) ◽  
pp. 7-13
Author(s):  
Theodora Agverianti ◽  
Muhartono Muhartono ◽  
Khairun Nisa Berawi
Keyword(s):  
Liver Damage ◽  
Histopathological Examination ◽  
Monosodium Glutamate ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Cell Degeneration ◽  
Control Group Design ◽  
Post Test

ABSTRACT Background: Long term consumption of Monosodium Glutamate (MSG) in excess isreported induced liver damage. Toxic effect in liver are due to the degeneration andnecrosis of hepatocytes, which also increases lipid peroxidation. Antioxidant in galangal rhizome is expected to repair liver damage caused by free radicals in MSG. Method: This study is an experimental research with Post Test Only Control Group Design. The samples in this study are 25 mice divided into 5 groups which are, negative control (not given any treatment), positive control (given MSG 4 mg/gBW for 14 days), treatment 1 (given MSG 4 mg/gBW for 14 days continued with ethanol extract of galangal rhizome 14 mg/20 gBW  for7 days), treatment 2 (given MSG 4 mg/gBW for 14 days continued with ethanol extract of galangal rhizome 28 mg/20 gBW for 7 days), and treatment 3 (given MSG 4 mg/gBW for 14 days continued with ethanol extract of galangal rhizome 56 mg/20 gBW  for 7 days). Then surgery was needed for histopathological examination. Results: Based on mean scoring results, the results showed calculation of liver cell degeneration for positive control (11,8), negative control (5,2), treatment 1 (10,6), treatment 2 (8,4), and treatment 3 (7,6). Based on the test results One Way ANOVA, the result showed the value of p=0,001 for liver histopathological appearance of mice. Conclusion: There is an effect of ethanol extract of galangal rhizome to the liver histopathological appearance of male mice.

scholarly journals UJI EFEKTIFITAS BIOLARVASIDA EKSTRAK ETANOL BUAH LAMPESU (Baccaurea lanceolata) TERHADAP LARVA INSTAR III Culex quinquefasciatus

2020 ◽  
Vol 3 (3) ◽  
pp. 7
Author(s):  
Dea Alfani Nandjan
Keyword(s):  
Culex Quinquefasciatus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Chemical Insecticide ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Infection Disease

Filariasis is chronic infection disease caused by worm and carried by Culex quinquefasciatus mosquito. One of theways to controlling mosquitoes vectors are use biolarvacide or chemical insecticide. Chemical insecticide causingresistance Culex quinquefasciatus larvae and toxic for human. This research aim to determine the biolarvacideeffectiviteness of ethanol extract lampesu fruit (Baccaurea lanceolata) to Culex quinquefasciatus larvae instars III.This study was true experimental with a Post test-only control group design. This study used 700 larvae instars III ofCulex quinquefasciatus divided into 7 groups consentration of 0,2%, 0,4%, 0,6%, 0,8% and 1%, the positive control(abate) and negative control (aquadest). The observation was did after treatment in 3 hours, 6 hours, 12  hours and 24hours. The experiment is replicated four times. At 24 hours exposure concentration of 0,6% the test larvae mortalityreached 38% and at concentration of 1% the test larvae mortality reached 30%. In this stuy the concentration of1,531 % was effective to kill larvae with of 50% mortality(LC50) and concentration of 10,729 % was effective to killlarvae with of 90% mortality(LC90). Ethanol Ekstract of Lampesu Fruit (Baccaurea lanceolata) not effective asbiolarvacide ofCulex quinquefasciatus larvae instars III.

scholarly journals Potential of Cocoa Ethanol Extract Toward Nematocyst Tubul Firing Inhibition of Physalia Utriculus Toxin In Vitro

2019 ◽  
Vol 5 (2) ◽  
pp. 30
Author(s):  
Sarwendah Siswi Winasis ◽  
Al Munawir ◽  
Adelia Handoko
Keyword(s):  
Normal Control ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Average Percentage ◽  
Post Test

There was estimated 150 million envenomation cases due to jellyfish stings occur globally every year. 100 from 10,000 jellyfish species in the world known to be dangerous, one them is Physalia utriculus. The aim of this study was to determine the potential of cocoa (Theobroma cacao L.) ethanol extract toward nematocyst tubul firing inhibition of jellyfish (Physalia utriculus) toxin in vitro. The method was true experimental design with post test only control group design. The study divided into 8 groups: 1 normal control, 1 positive control, 1 negative control, and 5 treatments grups by giving cocoa ethanol extract with concentration 20%, 2%, 0.2%, 0.02%, 0.002%. The observation was made by calculating the percentage number of firing nematocysts. The result showed average percentage of firing nematocyst in the normal control group was 42.50 ± 3.18, positive control group was 37.97 ± 5.57, negative control group was 52.44 ± 2.98, and treatment group which given with cocoa ethanol extract 20%, 2%, 0.2%, 0.02%, 0.002% were 48.24 ± 5.37; 40.62 ± 7.10; 29.45 ± 5.39; 37.60 ± 9.78; 41.11 ± 3.92, respectively. The One Way Annova statistical results test show significance value 0.001 (p≤0.05). The conclusion of this study was the cocoa ethanol extract with concentration 0.2% has most potential to inhibit the jellyfish (P. utriculus) nematocyst tubule firing in vitro. Keywords: Physalia utriculus, cocoa, toxin, jellyfish

scholarly journals Potensi Ekstrak Daun Kelor (Moringa Oleifera) sebagai Hepatoprotektor pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Dosis toksik

2018 ◽  
Vol 5 (1) ◽  
pp. 58
Author(s):  
Noer Kumala Indahsari ◽  
Masfufatun Masfufatun ◽  
Emilia Devi D.R
Keyword(s):  
Treatment Group ◽  
Moringa Oleifera ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
High Dose ◽  
Control Group Design ◽  
Post Test

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.

scholarly journals Hemostatic Effect of Ethanol Extract of Piper betle, Linn Leaves to Male Mice

Molekul ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 23
Author(s):  
Sadakata Sinulingga ◽  
Subandrate Subandrate ◽  
Bebbi Arisya Kesumaputri ◽  
Galuh Anggraini
Keyword(s):  
Bleeding Time ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Piper Betle ◽  
Control Group Design ◽  
Minimum Concentration ◽  
Group Design ◽  
Hemostatic Effect

Hemorrhage occurs in most of the dental care. Untreated hemorrhage could cause excessive blood loss, hypotension, and cyanosis. A Natural resource that reported has an hemostatic effect is ethanol extract of betel leaves (Piper betel, Linn).The aim of this study is to find the minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice. The experimental study used pretest-posttest with control group design was conducted on 35 mice that divided into 7 group which are negative control, positive control (feracrylum 1%), the ethanol extract of betel leaves 1%, 5%, 10%, 15%, and 20%. All mice were injected heparin intravenously. Mice’s tail was cut at diameter 3 mm and pretest bleeding time was counted. Mice’s tail was recut at diameter 4 mm, given treatment for 5 seconds and posttest bleeding time was counted. Results of paired t-test showed that reduction of bleeding time between pretest and posttest was significant (p<0,050). The enhancement of ethanol extract of betel leaves concentration leads to better hemostatic effect. Results of ANOVA test showed that comparison of posttest bleeding time among groups was significant (p<0,050). The minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice is 5%.

scholarly journals Pengaruh Pemberian Ekstrak Temulawak (Curcuma Xanthorrizha Roxb.) Terhadap Level Nekrosis pada Jaringan Lien Mencit Putih (Mus Musculus L.) Jantan Galur Balb/C yang Diinokulasi Plasmodium berghei anka

2019 ◽  
Vol 16 (2) ◽  
pp. 186
Author(s):  
MUHAMMAD REYHAN ARSYA ◽  
PRAWESTY DIAH UTAMI ◽  
IRMAWATI IRMAWATI
Keyword(s):  
Mus Musculus ◽  
Plasmodium Berghei ◽  
Histopathological Examination ◽  
Positive Control ◽  
Control Group ◽  
Control Group Design ◽  
Mortality And Morbidity ◽  
Treatment Groups ◽  
Post Test ◽  
Curcuma Xanthorrhiza

<p><strong>Abstract</strong></p><p><strong>Background : </strong>Malaria is a disease caused by the <em>Plasmodium</em> parasite and is transmitted by the <em>Anopheles</em> mosquito and is still a health problem in Indonesia due to high mortality and morbidity. One form of a severe complication of malaria in addition to cerebral malaria is a function failure of the spleen. Today, the management of malaria is increasingly limited due to resistance. Therefore, further development is needed to find new innovations in malaria treatment.</p><p><strong>Purpose : </strong>The purpose of this study was to determine the effect of temulawak rhizome extract (<em>Curcuma xanthorhizza</em> Roxb.) On the level of necrosis in the spleen tissue of male BALB / c mice (<em>Mus musculus</em> L.) inoculated with <em>Plasmodium berghei</em> ANKA.</p><p><strong>Methods :</strong>Experimental research used a post-test only control group design that used five groups of mice. One group of mice was left normal while the other four groups were inoculated with <em>Plasmodium berghei</em> ANKA, positive control groups were given aquades and three treatment groups treated with temulawak extract (<em>Curcuma xanthorrhiza</em> Roxb.) With a dose of 150 mg / KgBB, 100 mg / KgBB, and 50 mg / KgBB for four day. On the fifth day an observation of the level of necrosis in the spleen organ of mice to determine the level of necrosis by histopathological examination using a light microscope.</p><p><strong>Conclusion and Result : </strong>The results of this study indicate that the administration of ginger rhizome extract (<em>Curcuma xanthorriza</em> Roxb.) Has an influence on the level of necrosis of male mice (<em>Mus musculus</em> L.) BALB / c inoculated with <em>Plasmodium berghei</em> ANKA α = 0,002 (ρ&lt;0,05), where the administration of temulawak extract can increase necrosis levels compared to the control group . This is probably due to the lack of temulawak extract dosage and lack of observation in this study.</p><p> </p><p><strong>Keywords </strong>: Malaria, curcuma (<em>Curcuma xanthorrhiza</em> Roxb.), Necrosis level, <em>Plasmodium berghei</em> ANKA</p>

scholarly journals Efektivitas perasan daun seledri (Apium graveolens Linn.) sebagai pembersih gigi tiruan terhadap pertumbuhan Candida albicans pada basis gigi tiruan nilon termoplastikThe effectiveness of celery leaf juice (Apium graveolens Linn.) as a denture cleaner against the growth of Candida albicans on thermoplastic nylon denture base

2020 ◽  
Vol 32 (3) ◽  
pp. 184
Author(s):  
Dhilan Purna Aji ◽  
Achmad Gunadi ◽  
Tantin Ermawati
Keyword(s):  
Candida Albicans ◽  
Positive Control ◽  
Negative Control ◽  
Apium Graveolens ◽  
Control Group ◽  
Control Group Design ◽  
Denture Base ◽  
Significant Difference ◽  
Post Test ◽  
Leaf Juice

Pendahuluan: Basis gigi tiruan nilon termoplastik merupakan alternatif pengganti basis gigi tiruan resin akrilik. Pembersihan gigi tiruan diperlukan untuk menjaga basis gigi tiruan agar tetap bersih dari kontaminasi mikroorganisme. Daun seledri merupakan bahan alam yang memiliki kandungan flavonoid, saponin, tanin, dan minyak atsiri yang sudah terbukti bersifat antijamur. Penelitian sebelumnya hanya melihat zona hambat saja. Tujuan penelitian adalah menganalisis efektivitas perasan daun seledri sebagai pembersih gigi tiruan terhadap pertumbuhan Candida albicans pada basis gigi tiruan nilon termoplastik. Metode: Eksperimental laboratoris dengan rancangan penelitian post-test only control group design. Sampel nilon termoplastik berbentuk cakram dengan diameter 10 mm dan tebal 2 mm  sebanyak 25 dibagi menjadi 5 kelompok. Sampel dikontaminasi dengan C. albicans setelah direndam dalam kontrol negatif (akuades) selama 6 jam, kontrol positif (tablet effervescent) selama 15 menit, dan perasan daun seledri konsentrasi 25, 50, dan 100% selama 6 jam. Sampel selanjutnya diletakkan dalam media sabouraud dextrose broth (SDB). Pengujian efektivitas perasan daun seledri dilakukan dengan menentukan nilai absorbansi kekeruhan media SDB menggunakan spektrofotometer. Data dianalisis menggunakan one-way ANOVA dan Least Significant Difference (LSD). Hasil: Nilai konsentrasi C. albicans pada kelompok kontrol negatif mendapatkan hasil 0,380 x 108 CFU/mL, kontrol positif mendapatkan hasil 0,310 x 108 CFU/mL, perasan daun seledri konsentrasi 25% mendapatkan hasil 0,243 x 108 CFU/mL, konsentrasi 50% mendapatkan hasil 0,160 x 108 CFU/mL, dan konsentrasi 100% mendapatkan hasil 0,236 x 108 CFU/mL. Simpulan: Perasan daun seledri efektif sebagai pembersih gigi tiruan untuk menghambat  pertumbuhan C. albicans, dengan konsentrasi yang efektif sebesar 50%.Kata kunci: Nilon termoplastik, perasan daun seledri, Candida albicans. ABSTRACTIntroduction: Thermoplastic nylon denture base is an alternative to the acrylic resin denture base. Denture cleaning is needed to keep the denture base clean from contamination by microorganisms. Celery leaves are natural ingredients that contain flavonoids, saponins, tannins, and essential oils that have been proven to have antifungal properties. Previous research only observed the inhibitory zone. This research was aimed to analyse the effectiveness of celery leaf juice as a denture cleaner against the growth of Candida albicans on thermoplastic nylon denture base. Methods: Experimental laboratory with a post-test only control group design was conducted towards 25 disc-shaped thermoplastic nylon samples with a diameter of 10 mm and a thickness of 2 mm, and divided into five groups. Samples were contaminated with C. albicans after immersion in the negative control (aquadest) for 6 hours, positive control (effervescent tablets) for 15 minutes, and celery leaf juice with the concentrations of 25, 50, and 100% for 6 hours. The sample was then placed in the Sabouraud-dextrose broth (SDB) medium. The effectiveness of celery leaf juice was tested by determining the absorbance value of SDB media turbidity using a spectrophotometer. Data were analysed using one-way ANOVA and Least Significant Difference (LSD). Results: The concentration value of C. albicans in the negative control group was 0.380 x 108 CFU / mL, the positive control was 0.310 x 108 CFU / mL, the juice of celery leaves with the concentration of 25% was 0.243 x 108 CFU / mL, the concentration of 50% was 0.160 x 108 CFU / mL, and the concentration of 100% was 0.236 x 108 CFU / mL. Conclusion: Celery leaf juice is effective as a denture cleaner to inhibit the growth of C. albicans, with an effective concentration of 50%.Keywords: Thermoplastic nylon, celery leaf juice, Candida albicans.

scholarly journals Pengaruh Pemberian Ekstrak Etanol Buah Masisin (Rhodomyrtus tomentosa Wight) terhadap Frekuensi, Konsistensi, dan Durasi Diare pada Hewan Coba Mencit Putih Jantan (Mus musculus) yang Diinduksi Oleum Ricini

2019 ◽  
Vol 5 (1) ◽  
pp. 22-40
Author(s):  
Angeline Novia Toemon ◽  
Austin Bertilova Carmelita ◽  
Astri Widiarti ◽  
Dian Mutiasari
Keyword(s):  
Mus Musculus ◽  
Positive Control ◽  
Negative Control ◽  
Study Data ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Ethanol Extracts ◽  
One Way Anova ◽  
Rhodomyrtus Tomentosa

People in Central Borneo which is often used to treat diarrhea is the fruit of masisin (Rhodomyrtus tomentosa Wight) with active compounds which are capable to treat diarrhea such as alkaloids, flavonoids, tannins, saponins, and steroids. Diarrhea is a defecate with a liquid or half-liquid feces, the content of water in a feces is more than usual, it is more than 200 g or 200 ml / 24 hours. To know the effectiveness of the extract of the fruit of masisin as an alternative to antidiarrheal treatment seen from the frequency, duration, and consistency of feces in male white mice (Mus musculus). This study design was a true experimental design with post-test control group design with 5 treatments for each group. This study started by making some extracts of Masisin fruit. Followed observation with 5 treatment groups animals. The induction of diarrhea was using oleum ricini, with aquadest as the negative control, and Loperamide HCL as the positive control. The dose of ethanol extracts of masisin fruit divided into 0.3 mg/g BW, 0.6 mg/g BW and 1.2 mg/g BW, all of the treatments were given orally. This study data was statistically analyzed with one way ANOVA. The results of one way ANOVA test showed that the value of p <0,05 meaning that there is the influence of EEBM on frequency, consistency and duration of diarrhea at doses 0.3 mg/g BW, 0.6 mg/g BW and 1.2 mg/Gbw. Based on observations ethanol extracts of masisin fruit had antidiarrheal effect at doses 0,3 mg/g BW, 0.6 mg/g BW and 1.2 mg/g BW in male white mice (Mus musculus).

scholarly journals PENGARUH EKSTRAK ETANOL BIJI JINTEN HITAM (Nigella Sativa) TERHADAP EKSPRESI ET-1 AORTA PADA MENCIT MODEL PREEKLAMPSIA

2016 ◽  
Vol 2 (1) ◽  
pp. 37
Author(s):  
Rubiati Hipni
Keyword(s):  
Nigella Sativa ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Black Cumin ◽  
Group Design ◽  
Model Mouse ◽  
One Way Anova

<p style="text-align: justify;">Abstract: The objective of this research is to analyze the effects of ethanol extract of black cumin seeds (Nigella Sativa) to the expression of ET-1 Aorta given to the preeclampsia model mouse. The research design utilizes experimental and the kind of design used is posttest only control group design. Mice preeclampsia model. This research is divided into 6 groups, namely: Negative control, Positive control, Model + extract of black cumin seeds 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day. After conducting the surgery to the mouse, an examination is done to the expression of ET-1 Aorta by using Immunohistochemistry method. one way anova Were used as statistical analysis. There was an effect of treatment of giving the ethanol extract black cumin seeds of ET-1 Aorta to the preeclampsia model mouse (p<0,05).these doses of 500 mg, 1000mg, 1500mg, and 2000mg, ethanol extract of black cumin seeds has ability to decrease the ET-1 Aorta in preeclampsia model mouse. dose of 1500mg/KgBB/day is the most optimum dosage to reduce when compared to another dosages. Ethanol extract of black cumin seeds (Nigella Sativa) can decrease the expression of ET-1 Aorta in preeclampsia model mouse, particulary at dose 2000mg/Kg/BB/day sequentially Keyword : Preeclampsia; black cumin seeds (Nigella Sativa); ET-1; Aorta . Abstrak: Penelitian ini bertujuan untuk menganalisa pengaruh pemberian ekstrak etanol biji jinten hitam(Nigella Sativa) terhadap ekspresi ET-1 Aorta pada mencit model preeclampsia. Desain Penelitian ini menggunakan eksprimental (experimental) dengan jenis rancangan posttest only control group design. Menggunakan mencit model preeklampsia. Penelitian ini dibagi menjadi 6 kelompok yaitu Kontrol negatif, kontrol positif(Model), Model+ekstrak biji jinten hitam 500 mg/KgBB/hari, 1000 mg/KgBB/hari, 1500 mg/KgBB/hari, dan 2000 mg/KgBB/hari, setelah dilakukan pembedahan pada mencit kemudian dilakukan pemeriksaan ekspresi ET-1 Aorta dengan metode Immunohistokimia. Data hasil pengamatan dianalisis dengan uji one way anova. Ada pengaruh pemberian ekstrak etanol biji jinten hitam terhadap ekspresi ET-1 Aorta pada mencit model preeklampsia (p<0,05). Dosis 500mg, 1000mg, 1500mg, dan 2000mg jinten hitam mempunyai kemampuan terhadap penurunan ekspresi ET-1 Aorta Dosis yang paling optimum menurunkan adalah dosis 2000mg/KgBB/hari dibanding dosis yang lain. Ekstrak etanol biji jinten hitam (Nigella Sativa) dapat menurunkan ekspresi ET-1 Aorta pada mencit model preeklampsia terutama pada dosis 2000mg/KgBB/hari. Kata Kunci: Preeklampsia; Biji jinten hitam (Nigella sativa); ET-1; Aorta

scholarly journals Pengaruh Pemberian Ekstrak Rosella (Hibiscus sabdariffa Linn) Terhadap Kadar Malondialdehid dan Aktivitas Katalase Tikus yang Terpapar Karbon Tetraklorida

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Zuraida Zuraida ◽  
Eti Yerizel ◽  
Eliza Anas
Keyword(s):  
Vitamin C ◽  
Negative Control Group ◽  
Organ Damage ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Hibiscus Sabdariffa ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Abstrak Pemberian rosella (Hibiscus sabdariffa Linn) diharapkan melindung hepar tikus dari kerusakkan akibat stres oksidatif pada keracunan karbon tetraklorida (CCl 4). Senyawa yang sering dijadikan petunjuk adanya kerusakan tersebut adalah malondialdehid (MDA). Rosella mengandung vitamin C, flavonoid, polifenol dan beta karoten. Tujuanpenelitian ini adalah menentukan pengaruh pemberian ekstrak rosella (Hibiscus sabdariffa Linn) terhadap MDA dan aktivitas katalase tikus yang terpapar CCl 4. Ini adalah penelitian eksperimental dengan desain Post test Only Control Group Design. Sampel 24 ekor tikus Strain Wistar berumur 2-3 bulan, berat 150-200 gr. Sampel diambil secara acakdan dibagi 4 kelompok terdiri dari kelompok kontrol negatif, kontrol positif (CCl 4), perlakuan 1 (CCl 4 dan ekstrak rosella 250 mg/kg bb) dan perlakuan 2 (CCl4  dan ekstrak rosella 500 mg/kg bb). Pemberian CCl 4secara oral dosis tunggal, setelah 24 jam kemudian diberi ekstrak rosella secara oral selama 14 hari. Data dianalisis dengan uji Anova, tingkatkepercayaan 95%.Pemberian ekstrak rosella secara statistik didapatkan perbedaan yang signifikan rerata kadar MDA dan katalase antar kelompok (p < 0,05). Disimpulkan bahwa ekstrak rosella dapat menurunkan kadar MDA dan meningkatkan aktivitas katalase tikus yang terpapar CCl 4. Kata kunci: karbon tetraklorida, MDA, katalase, rosella Abstract Administering roselle (Hibiscus sabdariffa Linn) is expected to protect rat liver from damage caused by oxidative stress in CCl4 poisoning. Rosella contains vitamin C, flavonoids, polyphenol and beta carotene. Compounds which was often used as marker of the damage caused by free radicals wa MDA. The objective of this study was to determine the effect of extracts of roselle (Hibiscus sabdariffa Linn) on MDA and catalase activity of rats exposed to CCl4. Experimental research design with Post test Only Control Group Design. Samples of 24 male Wistar Strain rats were 2-3 months old. weighing 150-200 gr. Samples were taken at random and divided into 4 groups consisting of a negative control group, positive control (CCl4), treatment 1 (CCl4 and roselle extract 250 mg / kg bw) and treatment 2 (CCl4 and roselle extract 500 mg / kg bw). CCl4 was given a single dose orally, after 24 hours, the subjects were given rosella extract orally for 14 days. Data were analyzed by ANOVA with a confidence level of 95%. Rosella extract obtained statistically significant differences of MDA and catalase levels among groups (p <0.05). It can be concluded that the rosella extract can reduce levels of MDA and increase the activity of catalase mice exposed to CCl4. From the research it can be concluded that the rosella extract can reduce levels of MDA and increas the activity of catalase mice exposed to CCl4. It is needed further research on the toxicity of extracts of rosella and organ damage caused.Keywords: carbon tetrachloride, MDA, catalase, rosella

scholarly journals Uji daya hambat ekstrak daun bayam petik (Amaranthus hybridus L.) terhadap pertumbuhan bakteri Staphylococcus aureus

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Oktovianus Pormes ◽  
Damajanty H.C. Pangemanan ◽  
Michael A. Leman
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Amaranthus Hybridus ◽  
Group Design ◽  
Post Test

Abstract: Synthetic antibiotics have certain side effects, therefore, it is necessary to find alternative natural antibacterial materials which is easily available and to be cultivated, inter alia Amaranthus hybridus L. Its leaves contain active compounds, so they might have antibacterial potential. This study was aimed to determine the inhibitory effect of Amaranthus hybridus leaf extract on the growth of Staphylococcus aureus. This was a true experimental study using post test only control group design. This study was conducted at the Microbiology Laboratory of Faculty of Medicine and the Natural Phytochemical Laboratory of Faculty of Mathematics at Sam Ratulangi University. The modified Kirby-Bauer method was used with three wells, containing Amaranthus hybridus leaf extract, the positive control, and the negative control; and with 5 repetitions. The results showed that the average diameters of the inhibition zone of Amaranthus hybridus leaf extract and of the negative control were 0 mm meanwhile of erythromycin as the positive control was 38.8 mm. Conclusion: Amaranthus hybridus leaf extract had no inhibitory effect on Staphylococcus aureus. Keywords: inhibitory zone, Amaranthus hybridus L, Staphylococcus aureus Abstrak: Bahan antibiotik sintetik memiliki efek samping, sehingga perlu dicari bahan alternatif yaitu bahan alami yang mudah didapat dan dibudidayakan, salah satunya ialah bayam petik (Amaranthus hybridus L.). Daun bayam petik memiliki potensi antibakteri karena memiliki kandungan senyawa aktif. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak daun bayam petik terhadap pertumbuhan bakteri Staphylococcus aureus. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Penelitian dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran dan Labaratorium Fitokimia Fakultas MIPA Unsrat. Metode pengujian yang digunakan ialah modifikasi Kirby-Bauer dengan menggunakan tiga buah sumuran yang diberi ekstrak daun bayam petik, kontrol positif, dan kontrol negatif, sebanyak 5 kali pengulangan. Hasil penelitian menunjukan diameter rerata dari zona hambat yang terbentuk pada sumur dengan ekstrak daun bayam petik dan pada sumur dengan kontrol negatif ialah 0 mm, sedangkan pada sumur yang diberi kontrol positif amoksisilin terjadi resistensi sehingga diganti dengan eritromisin dan didapatkan rerata zona hambat ialah 38,8 mm. Simpulan: Ekstrak daun bayam petik (Amaranthus hybridus L.) tidak memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus.Kata kunci: daya hambat, Amaranthus hybridus L, Staphylococcus aureus

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