Efektivitas perasan daun seledri (Apium graveolens Linn.) sebagai pembersih gigi tiruan terhadap pertumbuhan Candida albicans pada basis gigi tiruan nilon termoplastikThe effectiveness of celery leaf juice (Apium graveolens Linn.) as a denture cleaner against the growth of Candida albicans on thermoplastic nylon denture base

Pendahuluan: Basis gigi tiruan nilon termoplastik merupakan alternatif pengganti basis gigi tiruan resin akrilik. Pembersihan gigi tiruan diperlukan untuk menjaga basis gigi tiruan agar tetap bersih dari kontaminasi mikroorganisme. Daun seledri merupakan bahan alam yang memiliki kandungan flavonoid, saponin, tanin, dan minyak atsiri yang sudah terbukti bersifat antijamur. Penelitian sebelumnya hanya melihat zona hambat saja. Tujuan penelitian adalah menganalisis efektivitas perasan daun seledri sebagai pembersih gigi tiruan terhadap pertumbuhan Candida albicans pada basis gigi tiruan nilon termoplastik. Metode: Eksperimental laboratoris dengan rancangan penelitian post-test only control group design. Sampel nilon termoplastik berbentuk cakram dengan diameter 10 mm dan tebal 2 mm sebanyak 25 dibagi menjadi 5 kelompok. Sampel dikontaminasi dengan C. albicans setelah direndam dalam kontrol negatif (akuades) selama 6 jam, kontrol positif (tablet effervescent) selama 15 menit, dan perasan daun seledri konsentrasi 25, 50, dan 100% selama 6 jam. Sampel selanjutnya diletakkan dalam media sabouraud dextrose broth (SDB). Pengujian efektivitas perasan daun seledri dilakukan dengan menentukan nilai absorbansi kekeruhan media SDB menggunakan spektrofotometer. Data dianalisis menggunakan one-way ANOVA dan Least Significant Difference (LSD). Hasil: Nilai konsentrasi C. albicans pada kelompok kontrol negatif mendapatkan hasil 0,380 x 108 CFU/mL, kontrol positif mendapatkan hasil 0,310 x 108 CFU/mL, perasan daun seledri konsentrasi 25% mendapatkan hasil 0,243 x 108 CFU/mL, konsentrasi 50% mendapatkan hasil 0,160 x 108 CFU/mL, dan konsentrasi 100% mendapatkan hasil 0,236 x 108 CFU/mL. Simpulan: Perasan daun seledri efektif sebagai pembersih gigi tiruan untuk menghambat pertumbuhan C. albicans, dengan konsentrasi yang efektif sebesar 50%.Kata kunci: Nilon termoplastik, perasan daun seledri, Candida albicans. ABSTRACTIntroduction: Thermoplastic nylon denture base is an alternative to the acrylic resin denture base. Denture cleaning is needed to keep the denture base clean from contamination by microorganisms. Celery leaves are natural ingredients that contain flavonoids, saponins, tannins, and essential oils that have been proven to have antifungal properties. Previous research only observed the inhibitory zone. This research was aimed to analyse the effectiveness of celery leaf juice as a denture cleaner against the growth of Candida albicans on thermoplastic nylon denture base. Methods: Experimental laboratory with a post-test only control group design was conducted towards 25 disc-shaped thermoplastic nylon samples with a diameter of 10 mm and a thickness of 2 mm, and divided into five groups. Samples were contaminated with C. albicans after immersion in the negative control (aquadest) for 6 hours, positive control (effervescent tablets) for 15 minutes, and celery leaf juice with the concentrations of 25, 50, and 100% for 6 hours. The sample was then placed in the Sabouraud-dextrose broth (SDB) medium. The effectiveness of celery leaf juice was tested by determining the absorbance value of SDB media turbidity using a spectrophotometer. Data were analysed using one-way ANOVA and Least Significant Difference (LSD). Results: The concentration value of C. albicans in the negative control group was 0.380 x 108 CFU / mL, the positive control was 0.310 x 108 CFU / mL, the juice of celery leaves with the concentration of 25% was 0.243 x 108 CFU / mL, the concentration of 50% was 0.160 x 108 CFU / mL, and the concentration of 100% was 0.236 x 108 CFU / mL. Conclusion: Celery leaf juice is effective as a denture cleaner to inhibit the growth of C. albicans, with an effective concentration of 50%.Keywords: Thermoplastic nylon, celery leaf juice, Candida albicans.

Download Full-text

Pengaruh Perendaman Ekstrak Bunga Sepatu (Hibiscus rosa sinensis L.) terhadap Pertumbuhan Candida albicans pada Plat Resin Akrilik

e-GIGI ◽

10.35790/eg.9.2.2021.34104 ◽

2021 ◽

Vol 9 (2) ◽

pp. 159

Author(s):

Yuhi Syaula ◽

Arlita L. Antari ◽

Diah A. Purbaningrum

Keyword(s):

Candida Albicans ◽

Acrylic Resin ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Control Group Design ◽

Treatment Groups ◽

Hibiscus Rosa Sinensis ◽

Post Test ◽

Post Hoc

Abstract: Denture plate materials such as acrylic resin can induce adhesion of Candida albicans. Therefore, acrylic resin needs to be immersed in disinfectant. However, disinfectant can change its physical and mechanical properties, hence an alternative material is needed, such as hibiscus flower (Hibiscus rosa sinensis L.) due to its antifungal activity. This study was aimed to identify the effects of hibiscus flower extract and its concentrations towards the growth of C. albicans on acrylic resin plates. This was an experimental and laboratory study using the post-test only with control group design. Acrylic resins were immersed in suspension of C. albicans, then were divided into four groups, as follows: 62.5% and 75% hibiscus flower extract (group I and II), positive control (sodium hypochlorite), and negative control (sterile aquadest). Acrylic resins were cultured and incubated on SDA media for 24 hours then the number of colonies were calculated. The results showed that C. albicans colonies in the treatment groups I, II, negative control, and positive control were 495 CFU/ml, 571.25 CFU/mL, 1175 CFU/mL, 23.125 CFU/mL respective-ly. The Kruskal-Wallis test showed significant differences in number of colonies of C. albicans (p<0.05) among all groups The post hoc Mann-Whitney test showed that all groups were significantly different, except for treatment groups I towards II. In conclusion, extract of hibiscus flower (H. rosa sinensis L.) affected the growth of C. albicans on acrylic resin plates.Keywords: hibiscus flower; Hibiscus rosa sinensis L.; Candida albicans; acrylic resin Abstrak: Adanya bahan plat basis gigi tiruan seperti resin akrilik dapat memicu perlekatan C. albicans; oleh karena itu, resin akrilik perlu direndam dalam larutan desinfektan. Namun, larutan desinfektan dapat mengubah sifat fisik dan mekanik dari akrilik sehingga diperlukan adanya bahan alternatif, antara lain bunga sepatu (Hibiscus rosa sinensis L.) yang memiliki aktivitas antifungal. Penelitian ini bertujuan untuk mengetahui pengaruh perendaman ekstrak dan konsentrasi bunga sepatu terhadap pertumbuhan C. albicans pada plat resin akrilik. Jenis penelitian ialah eksperimental laboratorik dengan post-test only with control group design. Resin akrilik direndam dalam suspensi C. albicans, Terdapat empat kelompok perlakuan yaitu ekstrak bunga sepatu 62,5% dan 75%, kontrol positif (sodium hipoklorit), dan kontrol negatif (akuades steril). Resin akrilik dikultur dan diinkubasi pada media SDA selama 24 jam, kemudian jumlah koloni C. albicans dihitung. Hasil penelitian ini menunjukkan jumlah koloni C. albicans kelompok perlakuan I, II, kontrol negatif, dan positif sebanyak 495 CFU/ml, 571.25 CFU/mL, 1175 CFU/mL, 23.125 CFU/mL secara berurut. Uji Kruskal-Wallis menunjukkan perbedaan jumlah koloni C. albicans yang bermakna (p<0.05) antar semua kelompok. Uji post hoc Mann-Whitney menunjukkan semua kelompok berbeda bermakna, kecuali kelompok perlakuan I dengan II. Simpulan penelitian ini ialah ekstrak bunga sepatu (H. rosa sinensis L.) berpengaruh terhadap pertumbuhan C. albicans pada plat resin akrilik.Kata kunci: bunga sepatu; Hibiscus rosa sinensis L.; Candida albicans; resin akrilik

Download Full-text

Potensi Ekstrak Daun Kelor (Moringa Oleifera) sebagai Hepatoprotektor pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Dosis toksik

Jurnal Ilmiah Kedokteran Wijaya Kusuma ◽

10.30742/jikw.v5i1.6 ◽

2018 ◽

Vol 5 (1) ◽

pp. 58

Author(s):

Noer Kumala Indahsari ◽

Masfufatun Masfufatun ◽

Emilia Devi D.R

Keyword(s):

Treatment Group ◽

Moringa Oleifera ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Flavonoid Compound ◽

Control Group ◽

High Dose ◽

Control Group Design ◽

Post Test

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.

Download Full-text

Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

Journal of Islamic Medicine ◽

10.18860/jim.v1i2.4456 ◽

2017 ◽

Vol 1 (2) ◽

pp. 76-87

Author(s):

Muhammad Nur

Keyword(s):

Treatment Group ◽

Total Sample ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Control Group Design ◽

White Rats ◽

Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

Download Full-text

Porositas Lempeng Resin Akrilik Pasca Perendaman Rebusan Daun Sirih dan Kayu Siwak

Jurnal Vokasi Kesehatan ◽

10.30602/jvk.v4i1.122 ◽

2018 ◽

Vol 4 (1) ◽

pp. 39

Author(s):

Yustin Nur Khoiriyah

Keyword(s):

Acrylic Resin ◽

Negative Control ◽

Control Group ◽

Control Group Design ◽

Betel Leaf ◽

Significant Difference ◽

Antimicrobial Function ◽

Post Test ◽

Boiled Water ◽

One Way Anova

Abstract: Porosity Of Acrylic Resin After Immersion In The Boiled Water Of Betel Leaves And Siwak Wood. Denture acrylic resin can be a collection point for stain, tar, and plaque and this will adversely affect the oral health of the denture wearer. One way to clean the oral cavity of denture users is to use mouthwash and soak the teeth with a cleaning solution/denture cleanser. Mouthwash solutions and chemical-based cleaning solutions at relatively high prices, and affect the porosity of dentures. Therefore, the need for alternative materials that are safe, cheap, natural as well as having antimicrobial function without affecting the level of porosity of denture. This study was to determine the effect of the combination of boiled water of betel leaves and siwak wood to the porosity of the acrylic resin plate. This study was an experimental study, posttest only design with the control group has been done at the integrated laboratory of Poltekkes Tanjungkarang, July – December 2015. Data were analyzed by One Way ANOVA and continued with Least Significant Difference test. The results showed that the combination of betel leaves and siwak wood does not affect the porosity of the acrylic resin plate. The highest concentration of the combination of betel leaf water and siwak wood that did not differ significantly with negative control (aquades) was 75% with longest immersion period was 59 days. Abstrak: Porositas Lempeng Resin Akrilik Pasca Perendaman Rebusan Daun Sirih Dan Kayu Siwak. Gigi tiruan resin akrilik dapat menjadi tempat pengumpulan stain, tar, dan plak dan hal ini akan berpengaruh jelek terhadap kesehatan mulut pemakai gigi tiruan. Salah satu cara menjaga kebersihan rongga mulut pengguna gigi tiruan adalah dengan menggunakan obat kumur dan merendam gigi-tiruan tersebut dengan larutan pembersih/denture cleanser. Larutan obat kumur dan larutan pembersih berbahan dasar dari bahan kimia dengan harga yang relatif mahal, serta mempengaruhi porositas gigi tiruan. Oleh karena itu, perlu adanya bahan alternatif yang aman, murah, alami sekaligus memiliki fungsi antimikroba dengan tanpa mempengaruhi tingkat porositas gigi tiruan. Tujuan penelitian ini adalah untuk mengetahui pengaruh kombinasi air rebusan daun sirih dan kayu siwak terhadap porositas lempeng resin akrilik. Penelitian ini adalah penelitian eksperimental, rancangan post test only with control group design, dilaksanakan di Laboratorium Terpadu Poltekkes Tanjungkarang, Juli-Desember 2015. Analisis data dengan uji One Way ANOVA dan dilanjutkan uji Least Significant Difference. Hasil penelitian menunjukkan bahwa kombinasi air rebusan daun sirih dan kayu siwak tidak mempengaruhi porositas lempeng resin akrilik. Konsentrasi tertinggi dari kombinasi air rebusan daun sirih dan kayu siwak yang tidak berbeda signifikan dengan kontrol negatif (aquades) adalah 75% dengan lama perendaman terpanjang yaitu 59 hari.

Download Full-text

UJI EFEKTIFITAS BIOLARVASIDA EKSTRAK ETANOL BUAH LAMPESU (Baccaurea lanceolata) TERHADAP LARVA INSTAR III Culex quinquefasciatus

Herb-Medicine Journal ◽

10.30595/hmj.v3i3.6391 ◽

2020 ◽

Vol 3 (3) ◽

pp. 7

Author(s):

Dea Alfani Nandjan

Keyword(s):

Culex Quinquefasciatus ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Chemical Insecticide ◽

Control Group Design ◽

Group Design ◽

Post Test ◽

Infection Disease

Filariasis is chronic infection disease caused by worm and carried by Culex quinquefasciatus mosquito. One of theways to controlling mosquitoes vectors are use biolarvacide or chemical insecticide. Chemical insecticide causingresistance Culex quinquefasciatus larvae and toxic for human. This research aim to determine the biolarvacideeffectiviteness of ethanol extract lampesu fruit (Baccaurea lanceolata) to Culex quinquefasciatus larvae instars III.This study was true experimental with a Post test-only control group design. This study used 700 larvae instars III ofCulex quinquefasciatus divided into 7 groups consentration of 0,2%, 0,4%, 0,6%, 0,8% and 1%, the positive control(abate) and negative control (aquadest). The observation was did after treatment in 3 hours, 6 hours, 12 hours and 24hours. The experiment is replicated four times. At 24 hours exposure concentration of 0,6% the test larvae mortalityreached 38% and at concentration of 1% the test larvae mortality reached 30%. In this stuy the concentration of1,531 % was effective to kill larvae with of 50% mortality(LC50) and concentration of 10,729 % was effective to killlarvae with of 90% mortality(LC90). Ethanol Ekstract of Lampesu Fruit (Baccaurea lanceolata) not effective asbiolarvacide ofCulex quinquefasciatus larvae instars III.

Download Full-text

PENGARUH PEMBERIAN EKSTRAK METHANOL DAUN Acanthus ilicifolius TOPIKAL TERHADAP JUMLAH MAKROFAG PADA PENYEMBUHAN MODEL ORAL CANDIDIASIS DENGAN KONDISI IMUNOSUPRESI

DENTA ◽

10.30649/denta.v13i1.182 ◽

2019 ◽

Vol 13 (1) ◽

Author(s):

Dwi Andriani ◽

Diana Soesilo ◽

Ghora Setyawan

Keyword(s):

Candida Albicans ◽

Methanol Extract ◽

Oral Candidiasis ◽

Control Group ◽

Control Group Design ◽

Significant Difference ◽

Post Test ◽

Anti Inflammatory ◽

Corticosteroid Drugs ◽

Anti Fungal

Background: Corticosteroid has a strong anti-inflammatory effect, unfortunately long-term usege of corticosteroid drugs can suppressed immune system. This condition makes Candida albicans as an opportunistic phatogen caused Oral Candidiasis. Macrophages have an important role in the first defense against infection. Methanol extract of A.ilicifolius leaf has anti-fungal, anti-inflammatory compounds to fight the growth of Candida albicans. Purpose: To determine the effect of methanol extract of A.ilicifolius leaf theraphy to the amount of macrophages in the healing pricess of oral candidiasis models with immunosuppressed conditions. Method: This study was true experimental with post test only control group design. Thirty-six strain wistar, 3 months old, and 250 grams of weight which were given dexamethason 0.5 mg and tetracycline 0.5mg for 7days, after Candida albican(ATCC10231) was induced for 2 weeks (3 times/week). Rats were divided into 6 groups: untreated(K-), nystatin(K+), A.ilicifolius 8%(P1), A.ilicifolius 12%(P2), A.ilicifolius 16%(P3), A.ilicifolius 20%(P4 ) all groups were treated for 2 weeks. After being treated, the rats tongue biopsied to examine the macrophages with Haematoxylin-eosin staining and observed with microscope (400x magnification). Data analyzed statistically by One-way ANOVA. Results: the amount of macrophages at K+(47.60±4.16), P4(40.20±3.56), P3(36.20±1.92), P2(17.00±2.45) were greater than K-(15.60±4.56), P1(14.60±2.70). there are no significant difference between group K- with P1 and P2, P3 with P4. There are significant difference between group K- with K+, K- with P3 and P4. K+ with P1, P2, P3 and P4. Group P1 with P3 and P4. Group P2 with P3 and P4. Conclusion: A.ilicifolius extract can increase the amount of macrophages in oral candidiasis immunosuppression models

Download Full-text

EFEKTIVITAS EKSTRAK DAUN JERUK PURUT (Citrus hystrix) SEBAGAI INSEKTISIDA ALAMI TERHADAP KECOAK AMERIKA (Periplaneta americana) DENGAN METODE SEMPROT

JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan ◽

10.22437/jmj.v8i1.9420 ◽

2020 ◽

Vol 8 (1) ◽

pp. 8-14

Author(s):

Hanina Hanina ◽

Sarah Madeleyne Baringbing

Keyword(s):

Leaf Extract ◽

Periplaneta Americana ◽

Positive Control ◽

Negative Control ◽

Probit Analysis ◽

Observation Time ◽

American Cockroach ◽

Control Group ◽

Significant Difference ◽

Post Test

ABSTRACT Cockroach control can be done in various ways one of which is spraying insecticide. Natural insecticides are less dangerous than conventional insecticides. This study aimed to determined the effectiveness of kaffir lime leaf extract (Citrus hystrix) as a natural insecticide against cockroach americana (Periplaneta americana) with spray method. This research was an experimental study with a post-test only control group designed design. The sample in this study was 60 cockroaches, the sample was divided into 6 groups: negative control (aquades), positive control (transflutrin 0.06%) and and 4 test groups with concentrations of kaffir lime leaf extract (20%, 40%, 60% , and 80%) each group consisted of 10 cockroaches. Phytochemical tests were carried out to determine the content of secondary metabolites in extracts of kaffir lime leaves. Positive phytochemical test results containing flavonoids, alkaloids and essential oils. Based on probit analysis, it is known that the LC50 value = 58.5%; LC95 = 85.1%. The time of death of LT50 is fastest at a concentration of 60% (3,036 hours) and LT95 is the fastest at a concentration of 80% (5,960 hours). Anova test analysis results showed that the positive control group had no significant difference with kaffir lime leaf extract concentration of 80% Kaffir lime leaf extract has an insecticide effect, the higher the concentration of kaffir lime leaf extract, the more number of dead cockroaches and the longer the observation time, the number of cockroaches death will also increase. Keywords: American cockroach, kaffir lime leaf extract, cockroach death ABSTRAK Pengendalian kecoa dapat dilakukan dengan berbagai cara salah satunya penyemprotan insektisida. Insektisida alami kurang berbahaya dibanding insektisida konvensional. Penelitian ini bertujuan untuk mengetahui efektifitas ekstrak daun jeruk purut (Citrus hystrix) sebagai insektisida alami terhadap kecoak americana (Periplaneta americana) dengan metode semprot. Jenis penelitian ini adalah penelitian eksperimental dengan rancangan post-test only control group designed. Sampel dalam penelitian ini adalah 60 ekor kecoak, sampel dibagi menjadi 6 kelompok yaitu kontrol negatif (aquades), kontrol positif (transflutrin 0,06%) dan dan 4 kelompok uji dengan konsentrasi ekstrak daun jeruk purut (20%, 40%, 60%, dan 80%) masing-masing kelompok terdiri dari 10 ekor kecoak. Uji fitokimia dilakukan untuk mengetahui kandungan senyawa metabolit sekunder pada ekstrak daun jeruk purut. Hasil uji fitokimia positif mengandung flavonoid, alkaloid dan minyak atsiri. Berdasarkan analisis probit, diketahui nilai LC50 = 58,5%; LC95 = 85,1%. Waktu kematian LT50 paling cepat pada konsentrasi 60% (3,036 jam) dan LT95 paling cepat pada konsentrasi 80% (5,960 jam). Hasil analisis uji anova menunjukkan bahwa kelompok kontrol positif tidak terdapat perbedaan yang signifikan dengan ekstrak daun jeruk purut konsentrasi 80% Ekstrak daun jeruk purut memiliki efek insektisida, semakin tinggi konsentrasi ekstrak daun jeruk purut maka jumlah kecoak yang mati semakin banyak dan semakin lama waktu pengamatan, maka jumlah kecoak yang mati juga akan semakin banyak. Kata kunci : kecoak amerika, ekstrak daun jeruk purut, kematian kecoak

Download Full-text

EFEKTIVITAS SUPLEMENTASI TERIPANG EMAS (Stichopus hermanii) DALAM MENCEGAH TERJADINYA ORAL CANDIDIASIS PADA TIKUS WISTAR

DENTA ◽

10.30649/denta.v12i1.155 ◽

2018 ◽

Vol 12 (1) ◽

pp. 9

Author(s):

Endah Wahjuningsih ◽

Paramita Devi Oktaviani ◽

Dwi Andriani

Keyword(s):

Candida Albicans ◽

Oral Candidiasis ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Significant Difference ◽

Post Test ◽

Mail Address ◽

E Mail ◽

Tongue Epithelium

ABSTRACT Background: Candida albicans is normal flora of oral cavity that can be pathogenic due to predisposition influence so that trigger Oral candidiasis. Stichopus hermanii suspected as an Oral candidiasis therapy because it contain antioxidant compound, antitumor and antifungi. Objective: Analyzing the effectiveness of Stichopus hermanii supplementation as a protective effect of oral candidiasis in Rats exposure to smoke. Materials and Methods: This experiment is post test only group control design using 35 male rats divided into 5 groups(X). X1 (negative control), X2 (positive control), X3 (Stichopus hermanii powder 0,0225mg/kgBB), X4 (Stichopus hermanii powder 0,045mg/kgBB), X5 (Stichopus hermanii powder 0,09mg/kgBB). Candida albicans induced into the mouth by an oral swab using cotton bud 3 times a week for 8 weeks. Smoke exposure as a predisposition factor be given 3 bars per day for 8 weeks. The rats being killed and tongue biopsies measure the thickness of tongue epithelium. The obtained data analyzation using One Way ANOVA and LSD test. Result: There is a significant difference (p<0.05) between X1 and X2, X1 and X3, X1 and X4, X1 and X5, X2 and X3, X2 and X4, X2 and X5, X3 and X5. Conclusion: Adduction of Stichopus hermanii capable of reducing risk of Oral candidiasis. Supplementation of Stichopus hermanii powder 0,09mg/kgBB is the most effective reduce thickness of the tongue epithelium in the group that exposure to smoke and induced of Candida albicans.Keywords: Stichopus hermanii, Oral candidiasis, Tongue, Candida albicans, Thickness of Epithelium Correspondence: Endah Wahjuningsih, Laboratorium Biologi Oral Fakultas Kedokteran Gigi Universitas Hang Tuah, Jl, Arif Rahman Hakim 150, Surabaya, Indonesia. Ph 031-5945864, fax: 031-5912191, e-mail address: [email protected]

Download Full-text

Pengaruh Pemberian Ekstrak Etanol Buah Masisin (Rhodomyrtus tomentosa Wight) terhadap Frekuensi, Konsistensi, dan Durasi Diare pada Hewan Coba Mencit Putih Jantan (Mus musculus) yang Diinduksi Oleum Ricini

Jurnal Surya Medika ◽

10.33084/jsm.v5i1.943 ◽

2019 ◽

Vol 5 (1) ◽

pp. 22-40

Author(s):

Angeline Novia Toemon ◽

Austin Bertilova Carmelita ◽

Astri Widiarti ◽

Dian Mutiasari

Keyword(s):

Mus Musculus ◽

Positive Control ◽

Negative Control ◽

Study Data ◽

Control Group ◽

Control Group Design ◽

Post Test ◽

Ethanol Extracts ◽

One Way Anova ◽

Rhodomyrtus Tomentosa

People in Central Borneo which is often used to treat diarrhea is the fruit of masisin (Rhodomyrtus tomentosa Wight) with active compounds which are capable to treat diarrhea such as alkaloids, flavonoids, tannins, saponins, and steroids. Diarrhea is a defecate with a liquid or half-liquid feces, the content of water in a feces is more than usual, it is more than 200 g or 200 ml / 24 hours. To know the effectiveness of the extract of the fruit of masisin as an alternative to antidiarrheal treatment seen from the frequency, duration, and consistency of feces in male white mice (Mus musculus). This study design was a true experimental design with post-test control group design with 5 treatments for each group. This study started by making some extracts of Masisin fruit. Followed observation with 5 treatment groups animals. The induction of diarrhea was using oleum ricini, with aquadest as the negative control, and Loperamide HCL as the positive control. The dose of ethanol extracts of masisin fruit divided into 0.3 mg/g BW, 0.6 mg/g BW and 1.2 mg/g BW, all of the treatments were given orally. This study data was statistically analyzed with one way ANOVA. The results of one way ANOVA test showed that the value of p <0,05 meaning that there is the influence of EEBM on frequency, consistency and duration of diarrhea at doses 0.3 mg/g BW, 0.6 mg/g BW and 1.2 mg/Gbw. Based on observations ethanol extracts of masisin fruit had antidiarrheal effect at doses 0,3 mg/g BW, 0.6 mg/g BW and 1.2 mg/g BW in male white mice (Mus musculus).

Download Full-text

Effect of putri malu (Mimosa pudica) extract on Ascaris suum mortality in vitro

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f090201 ◽

2011 ◽

Vol 9 (2) ◽

pp. 33-37

Author(s):

MUHAMMAD ARIF NUR SYAHID ◽

CR. SITI UTARI ◽

SUTARMIADJI DJUMARGA

Keyword(s):

Ascaris Suum ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Mimosa Pudica ◽

Control Group Design ◽

Significance Level ◽

Death Time ◽

Significant Difference

Syahid MAN, Utari CRS, Djumarga S. 2011. Effect of putri malu extract (Mimosa pudica) on Ascaris suum mortality in vitro. Biofarmasi 9: 33-37. This study was to determine the influence of Mimosa pudica extract in Ascaris suum mortality. This research was a laboratory experiment, with a post-test only with control group design by using 140 adult A. suum, divided into seven groups. This research used NaCl 0.9% for a negative control, pirantel pamoat 5 mg/mL solution for a positive control, and five intervention by using 20%, 40%, 60%, 80% and 100% concentration of M. pudica extract. The observation was conducted in every two hours until worm death and it was started to be counted after all worm death. Data were analyzed with one-way ANOVA test continued with Least Significance Difference (LSD) by using SPP for Window Release 17 with a significance level p<0.05. The results showed that all A. suum death in 96 hours at negative control, 2 hours at positive control, 29.5 hours at 20% M. pudica extract, 24.5 hours at 40% M. pudica extract, 16 hours at 60% M. pudica extract, 12 hours at 80% M. pudica extract and 4 hours at 100% M. pudica extract. There was a significant difference in the death time of A. suum in all research groups. From the result of research, it could be concluded that the extract of putri malu had an effect on accelerating A. suum mortality time.

Download Full-text