scholarly journals Occurrence of Aflatoxin B1 in Animal Feed Collected from the Northeastern Area of Morocco

2021 ◽  
Vol 11 (4) ◽  
pp. 587-593
Author(s):  
Naoual Alahlah ◽  
Mohammed El Maadoudi ◽  
Nourredine Bouchriti ◽  
Nourredine Bouchriti ◽  
Réda Triqui ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
High Performance ◽  
Animal Feed ◽  
Mean Value ◽  
Poultry Feed ◽  
Fish Feed ◽  
Animal Products ◽  
Production Chain ◽  
Significant Difference ◽  
Feed Samples

The carry-over of contaminants from feed to animal products is an important issue in the animal production chain, therefore, the quality control of those animal products should include the control of the animal feed. The current study was carried out to assess the contamination levels of three types of animal feed (dairy animal feed, poultry feed, and fish feed) by Aflatoxin B1. A total of 68 animal feed samples were collected from the Northeastern Moroccan area (Tangier-Tétouan-AL Hoceima). The samples were extracted with a mixture of acetone/water. The sample extractions were filtered, diluted with phosphate-buffered saline, and applied to an immunoaffinity column. Aflatoxin B1 was eluted with methanol then analyzed by high-performance liquid chromatography with fluorescence detection, after post-column photochemical derivatization. The analytical results for the level of Aflatoxin B1 in the animal feed samples revealed an average presence of 44.12% for all analyzed samples. The concentrations were between 1.02 and 13.59 µg/Kg, with a mean value of 4.08 ± 3.11 µg/Kg. The results indicated that there was a significant difference across the three types of animal feeds regarding the concentrations of Aflatoxin B1.

scholarly journals DETERMINATION OF NATURAL RADIONUCLIDE IN PIG PRODUCTION CHAIN IN MACEDONIA BY GAMMA SPECTROMETRY

AGROFOR ◽  
2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Dimitar Nakov ◽  
Metodija Trajchev ◽  
Aleksandra Angjeleska ◽  
Katerina Belichovska ◽  
Nikola Pacinovski
Keyword(s):  
Specific Radioactivity ◽  
Natural Radionuclide ◽  
Animal Products ◽  
Production Chain ◽  
Significant Difference ◽  
Human Food Chain ◽  
Naturally Occurring Radionuclides ◽  
Result Show ◽  
Important Pathway

Exposure of animals to ionizing irradiation may be a important pathway fortransfer of radionuclides to human food chain, thereby adding to the exposureburden. Therefore, radiation control of animal feeds and animal products willreduce risk for radioactive hazards to human health. The study was carried out inorder to detect the natural radioactivity in edible parts of pigs, excrements andfeeds in one commercial pig breeding farm in Macedonia. Therefore, 40K, 212Pb,214Pb, 228Ac, 235U, 241Am, 212Bi, 214Bi, 232Th, 7Be and 226Ra were measured usinggamma spectrometry. Gamma spectrometer Canberra Packard with a high-puritygermanium detector and Marinelli beakers (1 l capacity) were used for the samplesmeasurement. The most prominent gamma energies observed in the spectrabelonged to the naturally occurring radionuclides 40K, 235U and 232Th. Othernuclides if present occurred infrequently at low levels. The result show that 40Kmade the largest contribution to the specific radioactivity in all the samples. Themean activity concentration of the 40K in edible organs (kidney and liver), muscle,excrements and feeds was: 73.39±9.109 Bq/kg; 111.26±3.88 Bq/kg; 298.80±38.51Bq/kg; 83.60±10.279 Bq/kg, respectively. The 235U and 232Th were detectible onlyin feed samples (0.53±0.293 Bq/kg; 163.69±23.791 Bq/kg, respectively) andsamples from excrements (0.25±0.021 Bq/kg; 58.17±1.062 Bq/kg, respectively).The other radionuclides were detected only in few samples and the measuredactivities were below the detection limit. If we take in consideration the activityconcentration of the most frequently occurred 40K found in all samples, than therewas statistical significant difference between radioactivity concentration in organs,muscle, excrements and feeds (p<0.001).

scholarly journals Analysis of Mycotoxins Contamination in Poultry Feeds Manufactured in Selected Provinces of South Africa Using UHPLC-MS/MS

Toxins ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 452 ◽  
Author(s):  
Sharon Maphala Mokubedi ◽  
Judith Zanele Phoku ◽  
Rumbidzai Naledi Changwa ◽  
Sefater Gbashi ◽  
Patrick Berka Njobeh
Keyword(s):  
South Africa ◽  
High Performance ◽  
Fumonisin B1 ◽  
Monomethyl Ether ◽  
Poultry Feed ◽  
Alternariol Monomethyl Ether ◽  
Triple Quadrupole Mass Spectrometer ◽  
Type A Trichothecenes ◽  
Contamination Levels ◽  
Feed Samples

A total of 105 different types of poultry feed samples from South Africa were simultaneously analysed for the presence of 16 mycotoxins using ultra-high-performance liquid chromatography coupled to a triple quadrupole mass spectrometer (UHPLC-MS/MS). The data revealed the presence of 16 mycotoxins in the various poultry feed samples. Fumonisin B1 (FB1) was the most dominant recovered from 100% of samples analysed at concentrations ranging between 38.7 and 7125.3 µg/kg. This was followed by zearalenone (ZEN) (range: 0.1–429 µg/kg) and deoxynivalenol (DON) (range: 2.5–154 µg/kg). Samples were also found to be contaminated with fumonisin B2 (FB2) (range: 0.7–125.1 µg/kg), fumonisin B3 (FB3) (range: 0.1–125.1 µg/kg), α-zearalenol (α-ZEL) (range: 0.6–20 µg/kg ), β-zearalenol (β-ZEL) (range: 0.2–22.1 µg/kg), 3-acetyldeoxynivalenol (3-ADON) (range: 0.1–12.9 µg/kg) and 15-acetyldeoxynivalenol (15-ADON) (range: 1.7–41.9 µg/kg). Alternaria mycotoxin, i.e., Alternariol monomethyl ether (AME) was recovered in 100% of samples at concentrations that ranged from 0.3–155.5 µg/kg. Aflatoxins (AFs) had an incidence rate of 92% with generally low concentration levels ranging from 0.1–3.7 µg/kg. Apart from these metabolites, 2 type A trichothecenes (THs), i.e., HT-2 toxin (HT-2) (range: 0.2–5.9 µg/kg) and T-2 toxin (T-2) (range: 0.1–15.3 µg/kg) were also detected. Mycotoxin contamination in South African poultry feed constitutes a concern as correspondingly high contamination levels, such as those observed herein are likely to affect birds, which can be accompanied by severe health implications, thus compromising animal productivity in the country. Such exposures, primarily to more than one mycotoxin concurrently, may elicit noticeable synergistic and or additive effects on poultry birds.

scholarly journals Determination of Aflatoxins in Feed by HPLC and PCR

2020 ◽  
pp. 315-323
Author(s):  
Areej Zuhair Azeez ◽  
Mrwa Thamer Hindi ◽  
Maha Muhamed Khudiar ◽  
Adel Saadi AL Saadi ◽  
Noor Ibrahim Khadim
Keyword(s):  
Aspergillus Flavus ◽  
Aflatoxin B1 ◽  
High Performance ◽  
Animal Feed ◽  
Barley Grain ◽  
Specific Primers ◽  
Endogenous Gene ◽  
Three Samples ◽  
Food And Feed

The aim of this study is to identify aflatoxin secretion isolates in animal feeds by using HPLC and PCR  methods . In this study we collected fourty three samples of animal feed from different sites in Iraq (maize ,soybean ,sunflower grain ,barley grain, wheat). we isolated fungi  on potato dextrose agar, Aspergillus flavus  fungi was isolated from this samples  and identified the enzyme activities were tested for this isolate. The detection and determination for aflatoxin secretion of the isolates were done by using High Performance Liquid Chromatography (HPLC) technique. Twelve isolates shown Aflatoxin B1 secretion. Polymrease chain reaction ( PCR) technique is an alternative method to detect for Aspergillus spp. strains that secret aflatoxin by using specific primers( ITS1) endogenous gene for  Aspergillus flavus  and (ord , nor) genes  for aflatoxin B1 secreation, the PCR technique  considered to be an important role for safety and quality in industrial food and feed.

scholarly journals A Limited Survey of Aflatoxins and Zearalenone in Feed and Feed Ingredients from Pakistan

2016 ◽  
Vol 79 (10) ◽  
pp. 1798-1801 ◽  
Author(s):  
SHAHZAD ZAFAR IQBAL ◽  
MUHAMMAD RAFIQUE ASI ◽  
SONIA NISAR ◽  
KHALID MAHMOOD ZIA ◽  
SELAMAT JINAP ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Poultry Feed ◽  
Fluorescence Detector ◽  
Limit Of Quantification ◽  
Feed Ingredients ◽  
Cotton Seed Meal ◽  
The Mean ◽  
Mean Concentration ◽  
Feed Samples

ABSTRACT This work presents current information on the presence of aflatoxins (AFs) and zearalenone (ZEN) in feed and feed ingredients from Punjab, Pakistan. The 105 samples tested were concentrated feed, i.e., cotton seed meal (18 samples) and soybean meal (14), and feed ingredients, i.e., crushed corn (17), crushed wheat (15), barley (17). and poultry feed (24). Samples were analyzed using high-performance liquid chromatography equipped with a fluorescence detector. Analysis revealed that 69 of 105 samples were contaminated with AFs, and the highest mean concentrations of AFB1 (6.20 μg/kg) and total AFs (9.30 μg/kg) were found in poultry feed samples. The mean total AF concentrations ranged from the limit of quantification to 165.5 μg/kg. However, 75 of the 105 samples were positive for ZEN. The highest mean concentration (19.45 μg/kg) was found in poultry feed samples. The mean ZEN concentrations were 0.15 to 145.30 μg/kg. The prevalence of AFs and ZEN was high in feed and feed ingredients and needs urgent attention.

Transfer of aflatoxins from naturally contaminated feed to milk of Nili-Ravi buffaloes fed a mycotoxin binder

2016 ◽  
Vol 56 (10) ◽  
pp. 1637 ◽  
Author(s):  
N. Aslam ◽  
I. Rodrigues ◽  
D. M. McGill ◽  
H. M. Warriach ◽  
A. Cowling ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
High Performance ◽  
Mean Value ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Treatment Groups ◽  
Low Concentrations ◽  
Daily Concentration ◽  
The Mean ◽  
Carry Over

The objectives of this study were to observe the extent of transfer of aflatoxin B1 in feed to the aflatoxin M1 metabolite in milk in Nili-Ravi buffaloes and to evaluate the efficacy of a commercial mycotoxin binder (Mycofix, Biomin Singapore) incorporated into feed to minimise this transfer. Multiparous animals (n = 28) were randomly distributed to four groups corresponding to two treatments each with two levels of aflatoxin B1. Individual animals were exposed to naturally contaminated feed providing a total of 1475 µg/day (Groups A and B) or 2950 µg/day (Groups C and D) of aflatoxin B1. Groups B and D were given 50 g of mycotoxin binder daily mixed with feed whereas Groups A and C were kept as controls. Feed samples were analysed by reverse phase high performance liquid chromatography for aflatoxin B1 and milk samples were evaluated by enzyme-linked immunosorbent assay for the liver metabolite aflatoxin M1. The mean value of total daily aflatoxin M1 excretion for animals fed 2950 µg/day of aflatoxin B1 (112.6 µg/day) was almost double (P < 0.001) than the excretion in buffaloes fed 1475 µg/day (62.2 µg/day). The mean daily concentration of aflatoxin M1 in milk of animals from both treatment groups supplemented with 50 g/day of mycotoxin binder was 76.5 µg/day, nearly 22 µg lower than those without binder at 98.3 µg/day (s.e.d. = 5.99: P < 0.01). The interaction of binder and treatment was not significant i.e. the 50 g/day of binder was able to sequester aflatoxin B1 with the same efficiency in groups fed with high and low concentrations of aflatoxin B1. Carry over was (3.44%) lower (P = 0.001) in animals supplemented with 50 g/day of mycotoxin binder than those fed no binder (4.60%). Thus buffaloes are highly efficient at transferring aflatoxins in feed to the aflatoxin M1 metabolite in milk, whereas mycotoxin binder is capable of alleviating without preventing this contamination risk.

scholarly journals A Rapid and Sensitive Fluorescent Microsphere-Based Lateral Flow Immunoassay for Determination of Aflatoxin B1 in Distillers’ Grains

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2109
Author(s):  
Zifei Wang ◽  
Pengjie Luo ◽  
Baodong Zheng
Keyword(s):  
Aflatoxin B1 ◽  
High Performance ◽  
Animal Feed ◽  
Limit Of Detection ◽  
Lateral Flow ◽  
Distillers Grains ◽  
Detection Methods ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Fluorescent Microsphere

Aflatoxin B1 (AFB1) is a toxic compound naturally produced by the genera Aspergillus. Distillers’ grains can be used as animal feed since they have high content of crude protein and other nutrients. However, they are easily contaminated by mycotoxins, and currently there are no rapid detection methods for AFB1 in distillers’ grains. In this study, a lateral flow immunoassay (LFIA) based on red fluorescent microsphere (FM), is developed for quantitative detection of AFB1 in distillers’ grains. The whole test can be completed within 15 min, with the cut-off value being 25.0 μg/kg, and the quantitative limit of detection (qLOD) being 3.4 μg/kg. This method represents satisfactory recoveries of 95.2–113.0%, and the coefficients of variation (CVs) are less than 7.0%. Furthermore, this technique is successfully used to analyze AFB1 in real samples, and the results indicates good consistency with that of high-performance liquid chromatography (HPLC). The correlation coefficient is found to be greater than 0.99. The proposed test strip facilitates on-site, cost-effective, and sensitive monitoring of AFB1 in distillers’ grains.

scholarly journals Prevalence of Aflatoxin B1 and B2 in Poultary Feed

2014 ◽  
Vol 9 ◽  
pp. 109-112
Author(s):  
Sita R. Aryal ◽  
Durga Karki
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Aflatoxin B1 ◽  
Rainy Season ◽  
Winter Season ◽  
Poultry Feed ◽  
Permissible Level ◽  
Aflatoxin B2 ◽  
Layer Chromatography ◽  
Feed Samples

A total of 65 poultry feed samples were examined for the detection of aflatoxin (aflatoxin B1 and aflatoxin B2) using thin layer chromatography (TLC). Samples were collected from Chitwan and Kavrepalanchock districts. Out of those samples examined a total of 49 (75.38%) samples were found positive. Out of 49 (75.38%) samples positive, 42 (85.71%) samples were found positive both with aflatoxin B1 and B2 where as five (10.20%) samples were positive only with aflatoxin B1 and two (4.08%) samples were positive only with aflatoxin B2. Among them 13 (20%) samples were found positive having aflatoxin above permissible level. The concentration of aflatoxin in positive samples ranged from trace to 366 ppb (366 μg/kg). Likewise, out of 52 samples examined in rainy season, 40 samples (76.92%) were found positive where as out of 13 samples examined in winter season 9 (69.23%) were found positive.Nepal Agric. Res. J. Vol. 9, 2009, pp. 109-112DOI: http://dx.doi.org/10.3126/narj.v9i0.11648

scholarly journals Activity and Anti-Aflatoxigenic Effect of Indigenously Characterized Probiotic Lactobacilli against Aspergillus flavus—A Common Poultry Feed Contaminant

Animals ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 166 ◽  
Author(s):  
Nimra Azeem ◽  
Muhammad Nawaz ◽  
Aftab Ahmad Anjum ◽  
Shagufta Saeed ◽  
Saba Sana ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
High Performance ◽  
Animal Feed ◽  
Aflatoxin Production ◽  
Aflatoxin Contamination ◽  
Poultry Feed ◽  
Poultry Production ◽  
Ameliorative Effect ◽  
Probiotic Lactobacilli

Aflatoxin contamination in human food and animal feed is a threat to public safety. Aflatoxin B1 (AFB1) can be especially damaging to poultry production and consequently economic development of Pakistan. The present study assessed the in vitro binding of AFB1 by indigenously characterized probiotic lactobacilli. Six isolates (Lactobacillus gallinarum PDP 10, Lactobacillus reuetri FYP 38, Lactobacillus fermentum PDP 24, Lactobacillus gallinarum PL 53, Lactobacillus paracasei PL 120, and Lactobacillus gallinarum PL 149) were tested for activity against toxigenic Aspergillus flavus W-7.1 (AFB1 producer) by well diffusion assay. Only three isolates (PL 53, PL 120, and PL 149) had activity against A. flavus W-7.1. The ameliorative effect of these probiotic isolates on AFB1 production was determined by co-culturing fungus with lactobacilli for 12 days, followed by aflatoxin quantification by high-performance liquid chromatography. In vitro AFB1 binding capacities of lactobacilli were determined by their incubation with a standard amount of AFB1 in phosphate buffer saline at 37 °C for 2 h. AFB1 binding capacities of isolates ranged from 28–65%. Four isolates (PDP 10, PDP 24, PL 120, and PL 149) also ceased aflatoxin production completely, whereas PL 53 showed 55% reduction in AFB1 production as compared to control. The present study demonstrated Lactobacillus gallinarum PL 149 to be an effective candidate AFB1 binding agent against Aspergillus flavus. These findings further support the binding ability of lactic acid bacteria for dietary contaminants.

scholarly journals Reliable HPLC Determination of Aflatoxin M1 in Eggs

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Mostafa M. H. Khalil ◽  
Ahmed M. Gomaa ◽  
Ahmed Salem Sebaei
Keyword(s):  
Aflatoxin B1 ◽  
High Performance ◽  
Limit Of Detection ◽  
Aflatoxin M1 ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Animal Products ◽  
Liquid Chromatography Method ◽  
Different Levels

Aflatoxin M1 is the foremost metabolite of aflatoxin B1 in humans and animals, which may be present in animal products from animals fed with aflatoxin B1 contaminated feed. In this study a high performance liquid chromatography method for determination of aflatoxin M1 in eggs was described. The egg samples were diluted with warmed water and the toxin was immunoextracted followed by fluorescence detection. The average recovery of aflatoxin M1 at the three different levels 0.05, 0.1, and 0.5 μg/kg varied between 87% and 98%. The method is linear from the limit of quantification 0.05 μg/kg up to 3 μg/kg levels. This method is intended for aflatoxin M1 analyses in eggs simply with minimum toxin lose, excellent recovery, and accurate results with the limit of detection 0.01 μg/kg.

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