scholarly journals Molecular diagnosis and risk factors of canine ehrlichiosis in the municipality of Itabuna-Bahia, Brazil

2020 ◽  
Vol 41 (3) ◽  
pp. 897
Author(s):  
Jeane Martinha dos Anjos Cordeiro ◽  
Paula Elisa Brandão Guedes ◽  
Alexandre Dias Munhoz ◽  
Fabiana Lessa Silva
Keyword(s):  
Risk Factors ◽  
Nested Pcr ◽  
Clinical Signs ◽  
Rhipicephalus Sanguineus ◽  
Nested Polymerase Chain Reaction ◽  
Molecular Tests ◽  
Canine Ehrlichiosis ◽  
Non Governmental Organizations ◽  
Canine Monocytic Ehrlichiosis ◽  
The City

Ehrlichiosis is an emerging zoonosis worldwide and has had several adverse effects on public health. Canine monocytic ehrlichiosis (CME), caused by Ehrlichia canis, has the tick Rhipicephalus sanguineus as the vector. The main clinical signs in affected dogs are fever, apathy, anorexia, weight loss, and neurological signs. The diagnosis is made through the association of clinical signs with parasitological, serological, and molecular tests. The aim of this study was to evaluate the occurrence of E. canis infection in dogs from the city of Itabuna-Bahia, as well as to identify the risk factors related to infection. For this, 405 dogs from the Center for Zoonoses Control (CCZ), non-governmental organizations (NGOs), and dogs domiciled and semi-domiciled in the city of Itabuna, southern Bahia, were evaluated. After initial physical evaluation of the dogs, blood samples were collected by venipuncture for subsequent DNA extraction and E. canis testing using the nested Polymerase Chain Reaction (nested-PCR) technique. In addition, an epidemiological questionnaire that included questions related to the animals was administered to the dog owners to identify the risk factors for exposure to the etiological agent and to the vector. Approximately 17% of the dogs in the municipality of Itabuna-Bahia tested positive for E. canis by nested-PCR, a result higher than that found in other studies conducted in the same municipality. Among the factors associated with E. canis infection, contact with other dogs (p = 0.0226) was an important factor for the dissemination of CME, since dogs are reported to be reservoirs of E. canis. Male dogs (p = 0.0016) presented lower risk for E. canis infection. Other studies, however, describe no association between animal gender and infection by E. canis. Preventive measures to reduce exposure to the vector of ehrlichiosis are necessary.

scholarly journals Haematopathological Changes in Dogs Affected with Ehrlichia Canis in Lesvos

2017 ◽  
Vol 61 (2) ◽  
pp. 44-49 ◽  
Author(s):  
A. Geromichalou ◽  
Z. Faixová
Keyword(s):  
Clinical Signs ◽  
Rhipicephalus Sanguineus ◽  
Definitive Diagnosis ◽  
Biochemical Tests ◽  
Monocytic Cells ◽  
Canine Ehrlichiosis ◽  
Present Information ◽  
Canine Monocytic Ehrlichiosis ◽  
Tick Borne Disease ◽  
Protein Alterations

Abstract Canine Ehrlichiosis is an important immunosuppressive tick borne disease in dogs. The geographical distribution and transmission is mostly related with Rhipicephalus sanguineus which acts as a vector. There is no predilection of age or sex; all breeds may be infected with Canine Monocytic Ehrlichiosis (CME). The primary targets are monocytic cells. Platelet disorders and serum protein alterations are the principal hematological and biochemical consequences of infections. Clinical signs are almost non-specific. A definitive diagnosis requires: visualization of morulae within monocytes on cytology, detection of serum antibodies with E. canis, the IFA test, or the PCR. The objective of this study was to present information about haematological and biochemical tests of E. canis infected dogs in Lesvos island in Greece, which is an endemic area.

scholarly journals First report on molecular prevalence and identification of Anaplasma platys in dogs in Khon Kaen, Thailand

2021 ◽  
pp. 2613-2619
Author(s):  
Biethee Rani Sarker ◽  
Thongphet Mitpasa ◽  
Arayaporn Macotpet ◽  
Pattara-Anong Bupata ◽  
Somboon Sangmaneedet ◽  
...  
Keyword(s):  
Risk Factors ◽  
Platelet Count ◽  
Dna Sequencing ◽  
Similarity Index ◽  
Rhipicephalus Sanguineus ◽  
Severe Thrombocytopenia ◽  
Anaplasma Platys ◽  
Related Factors ◽  
Nested Polymerase Chain Reaction ◽  
Khon Kaen

Background and Aim: Anaplasma platys is a blood parasite that infects platelets, causing thrombocytopenia. Rhipicephalus sanguineus ticks are believed to transmit A. platys. To identify A. platys, nested polymerase chain reaction (PCR) has proven to be an effective diagnostic tool. In this study, the molecular prevalence of A. platys infection in dogs was investigated for the 1st time in the Khon Kaen region of Thailand. The association between risk factors and A. platys infection was also evaluated. Materials and Methods: A total of 130 blood samples were collected from dogs in Khon Kaen, Thailand. DNA from the samples was extracted and nested PCR was applied for molecular analysis. Platelet count and packed cell volume (PCV) levels were measured. Platelet counts were categorized into four grades: Non-thrombocytopenia (platelets >200,000 cells/μL), mild thrombocytopenia (platelets 150,000-200,000 cells/μL), moderate thrombocytopenia (platelets 100,000-150,000 cells/μL), and severe thrombocytopenia (platelets <100,000 cells/μL). Four categories for PCV levels of >37%, 30-37%, 20-29%, and <20% were defined as no anemia, mild anemia, moderate anemia, and severe anemia, respectively. DNA sequencing was analyzed using BTSeq™ (Barcode-Tagged Sequencing; CELEMICS, Seoul, South Korea) for similarity index. Results: Among the 130 samples, 9 (6.9%) were positive for A. platys infection. There was an association between low platelet count and infection (p<0.05). PCV level was also associated with A. platys infection (p<0.05). DNA sequencing results of the nine positive samples showed similarity to known sequences of A. platys with 99.36-100% nucleotide identity. These results suggested low genetic diversity in A. platys infecting dogs in the Khon Kaen area. Conclusion: By amplifying 16S rRNA, A. platys infection was detected in the blood of Thai dogs. Further work should be performed to identify risk factors potentially associated with A. platys infection in dogs in Khon Kaen. Other related factors should also be considered, such as location and breeding, as well as the environmental characteristics of each locality. In addition, sampling a larger number of animals may reveal predictors for the positivity of A. platys in dogs in this region.

scholarly journals Risk factors and clinical disorders of canine ehrlichiosis in the South of Bahia, Brazil

2011 ◽  
Vol 20 (3) ◽  
pp. 210-214 ◽  
Author(s):  
Renata Santiago Alberto Carlos ◽  
Fábio Santos Carvalho ◽  
Amauri Arias Wenceslau ◽  
Nadia Regina Pereira Almosny ◽  
George Rêgo Albuquerque
Keyword(s):  
Risk Factors ◽  
Dna Sequencing ◽  
Nested Pcr ◽  
Elisa Test ◽  
Diagnostic Methods ◽  
Blood Samples ◽  
Canine Ehrlichiosis ◽  
Blood Smears ◽  
Clinical Disorders ◽  
Dot Elisa

The aim of this work was to study the clinical disorders and risk factors of canine ehrlichiosis in Ilhéus and Itabuna, Bahia, and compare different diagnostic methods. Blood samples were collected from 200 dogs. Each dog was clinically examined. A questionnaire was used to evaluate the risk factors. The blood samples were analyzed using the Dot-ELISA test; hematometry, platelet counts and searches for morulae on blood smears were performed. Nested PCR was carried out on 50 serologically positive samples and 50 negative samples. Three positive PCRs were sequenced. Thirty-six percent were serologically positivity and 5.5% from blood smears. The animals were anemic and thrombocytopenic. Presence of ticks and living in areas on the urban periphery were considered to be risk factors (p < 0.05). Nested PCR identified 11 positive dogs of which nine were serologically positive and two were negative. The DNA sequencing was consistent with the presence of Ehrlichia canis.

scholarly journals Risk Factors Associated With the Presence of Mycobacterium Bovis in Macroscopic Lesions Suspected as Being Caused by Bovine Tuberculosis Detected in Slaughterhouses

2021 ◽  
Author(s):  
Flávio Gomes Barcelos ◽  
Tais Ramalho dos Anjos ◽  
Leila Auxiliadora de Arruda Alencar ◽  
Maria Júlia Sudária de Freitas ◽  
Mariano Martinez Espinosa ◽  
...  
Keyword(s):  
Risk Factors ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Statistical Significance ◽  
Control Measures ◽  
Epidemiological Surveillance ◽  
Nested Polymerase Chain Reaction ◽  
Mato Grosso ◽  
Molecular Tests

Abstract Mycobacterium bovis is a bacterium belonging to the Mycobacterium tuberculosis complex that causes tuberculosis in cattle and in other domestic and wild animals, as well as in humans. Disease control measures are carried out by slaughtering animals tested positive in the intradermal tuberculinization test and sanitation of their original living spaces, in addition to epidemiological surveillance carried out through the sanitary inspection of bovine carcasses in slaughterhouses. In the latter, official inspection services collect samples from macroscopic lesions suspected of bovine tuberculosis, which are then sent for laboratory analysis. Knowledge concerning the variables associated with the occurrence of M. bovis can aid in decision-making regarding control and disease eradication efforts. In this context, the aim of this study was to identify the risk factors for a positive M. bovis diagnosis in suspected bovine tuberculosis lesions obtained during epidemiological surveillance activities in the state of Mato Grosso, Brazil. A total of 105 suspicious lesions were analyzed using the Nested Polymerase Chain Reaction (nested q-PCR) method, of which 14 (13.33%) tested positive for M. bovis. Univariate and bivariate statistical analyses indicated that the variable “animal slaughter” was the only risk factor presenting statistical significance associated with the diagnosis of M. bovis (p < 0.05), demonstrating that macroscopic lesions suspected as being caused by bovine tuberculosis from animals with an in vivo diagnosis were 2.82 - fold more likely to result in a positive M. bovis diagnosis by molecular tests.

scholarly journals A comparative study of nested-PCR and direct agglutination test (DAT) for the detection of Leishmania infantum infection in symptomatic and asymptomatic domestic dogs

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yonas Yimam Ayene ◽  
Mehdi Mohebali ◽  
Homa Hajjaran ◽  
Behnaz Akhoundi ◽  
Saeedeh Shojaee ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Leishmania Infantum ◽  
Venous Blood ◽  
Clinical Signs ◽  
Agglutination Test ◽  
Domestic Dogs ◽  
Direct Agglutination Test ◽  
Nested Polymerase Chain Reaction ◽  
Endemic Areas ◽  
Visceral Leishmaniosis

Abstract Objective Canine visceral leishmaniasis (CVL) is the main source of human visceral leishmaniosis (HVL) in Mediterranean region, including Iran and is spread from domestic dogs to Phlebotomine sand flies vectors to humans. To control the transmission of HVL, early and accurate detection of infected dogs is paramount importance despite it remains a confronting challenge. Herein, we evaluated the performance of direct agglutination test (DAT) against gold standard nested polymerase chain reaction (nested-PCR) for CVL diagnosis in symptomatic and asymptomatic domestic dogs from endemic areas of Iran. Results Venous blood samples were collected from dogs without clinical signs (n  =  30) and with clinical signs (n  =  35) suggestive of Leishmania infantum infection. Among 65 samples examined, Leishmania DNA was detected by nested-PCR in 89.23% (58/65). Furthermore, 86.15% (56/65) nested-PCR positive samples were also DAT positive. The results of the DAT sensitivity test were 96.43% and 96.67% in symptomatic and asymptomatic dogs, respectively, while the specificity was 100.00% and 60.00% in symptomatic and asymptomatic dogs, respectively. The results of this study also pointed out substantial concordance between DAT test and nested-PCR method in both symptomatic dogs (Κ  =  0.783; P  <  0.001) and asymptomatic dogs (Κ  =  0.618; P  <  0.001). Thus, DAT represents as a simple and economic tool for initial diagnosis of CVL particularly in endemic areas of the disease.

scholarly journals Risk factors associated with the presence of Mycobacterium bovis in macroscopic lesions suspected as being caused by bovine tuberculosis detected in slaughterhouses

2022 ◽  
Vol 43 (2) ◽  
pp. 713-726
Author(s):  
Flávio Gomes Barcelos ◽  
◽  
Taís Ramalho dos Anjos ◽  
Leila Auxiliadora de Arruda Alencar ◽  
Vinicius Silva Castro ◽  
...  
Keyword(s):  
Risk Factors ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Statistical Significance ◽  
Control Measures ◽  
Epidemiological Surveillance ◽  
Nested Polymerase Chain Reaction ◽  
Mato Grosso ◽  
Molecular Tests

Mycobacterium bovis is a bacterium belonging to the Mycobacterium tuberculosis complex that causes tuberculosis in cattle and in other domestic and wild animals, as well as in humans. Disease control measures are carried out by slaughtering animals tested positive in the intradermal tuberculinization test and sanitation of their original living spaces, in addition to epidemiological surveillance carried out through the sanitary inspection of bovine carcasses in slaughterhouses. In the latter, official inspection services collect samples from macroscopic lesions suspected of bovine tuberculosis, which are then sent for laboratory analysis. Knowledge concerning the variables associated with the occurrence of M. bovis can aid in decision-making regarding control and disease eradication efforts. In this context, the aim of this study was to identify the risk factors for a positive M. bovis diagnosis in suspected bovine tuberculosis lesions obtained during epidemiological surveillance activities in the state of Mato Grosso, Brazil. A total of 105 suspicious lesions were analyzed using the Nested Polymerase Chain Reaction (nested q-PCR) method, of which 14 (13.33%) tested positive for M. bovis. Univariate and bivariate statistical analyses indicated that the variable “animal slaughter” was the only risk factor presenting statistical significance associated with the diagnosis of M. bovis (p < 0.05), demonstrating that macroscopic lesions suspected as being caused by bovine tuberculosis from animals with an in vivo diagnosis were 2.82 - fold more likely to result in a positive M. bovis diagnosis by molecular tests.

scholarly journals Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia

2021 ◽  
Vol 71 (2) ◽  
pp. 230-238
Author(s):  
Elena Atanaskova Petrov ◽  
Irena Celeska ◽  
Zagorka Popova ◽  
Kiril Krstevski ◽  
Igor Djadjovski
Keyword(s):  
Molecular Detection ◽  
Clinical Symptoms ◽  
Rhipicephalus Sanguineus ◽  
Ehrlichia Canis ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Positive Serology ◽  
Western Asia ◽  
Canine Monocytic Ehrlichiosis ◽  
The City

Abstract Canine monocytic ehrlichiosis (CME) is a widespread, tick-borne, canine disease, caused by an obligate intracellular bacterium, Ehrlichia canis. The main vector, a brown-dog tick, Rhipicephalus sanguineus, is widely distributed, especially in areas with tropic, subtropic, or Mediterranean climates (Central and South America, Eastern and Western Asia, Africa, Australia and Southern Europe). The study performed in 2012, by Stefanovska et al., determined a seroprevalence of 18.7% of E. canis among the Macedonian dog population. Up to date, the presence of E. canis, using molecular diagnostic methods, has not been investigated in Macedonia. Therefore, this study aimed to confirm the presence of E. canis, in the pet-dog population on the territory of the city of Skopje, North Macedonia, using a highly sensitive multiplex Real-Time PCR method (qPCR). Whole blood samples from 80 dogs of different breeds and ages, with clinical symptoms of CME and positive serology result for the presence of antibodies against E.canis, were collected for analyses. Out of 80 dogs, 36 (45%) were found as positive. The present work reports the first molecular detection of E. canis in pet dogs on the territory of the city of Skopje, Macedonia.

scholarly journals Low Maternal Immunoglobulin G Avidity and Single Parity as Adverse Implications of Human Cytomegalovirus Vertical Transmission in Pregnant Women with Immunoglobulin M Positivity

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 866
Author(s):  
Masatoki Kaneko ◽  
Junsuke Muraoka ◽  
Kazumi Kusumoto ◽  
Toshio Minematsu
Keyword(s):  
Risk Factors ◽  
Pregnant Women ◽  
Vertical Transmission ◽  
Human Cytomegalovirus ◽  
Multiple Logistic Regression Analysis ◽  
Clinical Signs ◽  
Immunoglobulin M ◽  
Igg Antibody ◽  
Neurological Sequelae ◽  
Previous Pregnancy

Human cytomegalovirus (CMV) is the leading cause of neurological sequelae in infants. Understanding the risk factors of primary CMV infection is crucial in establishing preventive strategies. Thus, we conducted a retrospective cohort study to identify risk factors of vertical transmission among pregnant women with immunoglobulin (Ig) M positivity. The study included 456 pregnant women with IgM positivity. Information on age, parity, occupation, clinical signs, IgM levels, and IgG avidity index (AI) was collected. The women were divided into infected and non-infected groups. The two groups showed significant differences in IgM level, IgG AI, number of women with low IgG AI, clinical signs, and number of pregnant women with single parity. In the multiple logistic regression analysis, pregnant women with single parity and low IgG AI were independent predictors. Among 40 women who tested negative for IgG antibody in their previous pregnancy, 20 showed low IgG AI in their current pregnancy. Among the 20 women, 4 had vertical transmission. These results provide better understanding of the risk factors of vertical transmission in pregnant women with IgM positivity.

scholarly journals The Implementation of Childhood Obesity Related Policy Interventions in Malaysia—A Non Communicable Diseases Scorecard Project

2021 ◽  
Vol 18 (11) ◽  
pp. 5950
Author(s):  
Wilfred Kok Hoe Mok ◽  
Noran Naqiah Hairi ◽  
Caryn Mei Hsien Chan ◽  
Feisul Idzwan Mustapha ◽  
Thamil Arasu Saminathan ◽  
...  
Keyword(s):  
Risk Factors ◽  
Childhood Obesity ◽  
Communicable Diseases ◽  
Overweight And Obesity ◽  
Current Status ◽  
Non Communicable Diseases ◽  
Modified Delphi ◽  
Policy Interventions ◽  
Non Governmental Organizations ◽  
The Government

(1) Background: The prevalence of overweight and obesity among children has increased tremendously in the ASEAN region, including Malaysia. In Malaysia, the National Strategic Plan for Non-Communicable Diseases (2015–2025) provides the overall framework for its response to the non-communicable diseases (NCD) epidemic. Preventing childhood obesity is one of the key strategies for early intervention to prevent NCDs. The objective of this research is to examine the current status of policy interventions in addressing childhood obesity in Malaysia. (2) Methods: A panel of 22 stakeholders and experts from Malaysia, representing the government, industry, academia and non-governmental organizations, were sampled using a modified Delphi technique. Data were collected using a modified NCD scorecard under four domains (governance, risk factors, surveillance and research and health systems response). A heat map was used to measure the success of the four realms of the NCD scorecard. For each domain of the NCD scorecard, the final score was grouped in quintiles. (3) Results: A total of 22 participants responded, comprising of eight (36.4%) males and 14 (63.4%) females. All the domains measured in implementing policies related to childhood obesity were of low progress. Nine governance indicators were reported as 22.5% (low progress), four in the risk factors domain, and two in the surveillance. This shows that timely and accurate monitoring, participatory review and evaluation, and effective remedies are necessary for a country’s surveillance system. (4) Conclusion: Although Malaysia has published several key strategic documents relating to childhood obesity and implemented numerous policy interventions, we have identified several gaps that must be addressed to leverage the whole-of-government and whole-of-society approach in addressing childhood obesity in the country.

scholarly journals Genomic investigation of a dengue virus outbreak in Thiès, Senegal, in 2018

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Amy Gaye ◽  
Tolla Ndiaye ◽  
Mouhamad Sy ◽  
Awa B. Deme ◽  
Alphonse B. Thiaw ◽  
...  
Keyword(s):  
West Africa ◽  
Dengue Virus ◽  
Clinical Signs ◽  
Health Concern ◽  
Public Health Concern ◽  
Local Circulation ◽  
Viral Genomes ◽  
Genomic Characterization ◽  
The City

AbstractDengue virus is a major and rapidly growing public health concern in tropic and subtropic regions across the globe. In late 2018, Senegal experienced its largest dengue virus outbreak to date, covering several regions. However, little is known about the genetic diversity of dengue virus (DENV) in Senegal. Here we report complete viral genomes from 17 previously undetected DENV cases from the city of Thiès. In total we identified 19 cases of DENV in a cohort of 198 individuals with fever collected in October and November 2018. We detected 3 co-circulating serotypes; DENV 3 was the most frequent accounting for 11/17 sequences (65%), 4 (23%) were DENV2 and 2 (12%) were DENV1. Sequences were most similar to recent sequences from West Africa, suggesting ongoing local circulation of viral populations; however, detailed inference is limited by the scarcity of available genomic data. We did not find clear associations with reported clinical signs or symptoms, highlighting the importance of testing for diagnosing febrile diseases. Overall, these findings expand the known range of DENV in Senegal, and underscore the need for better genomic characterization of DENV in West Africa.

Sign in / Sign up

Export Citation Format

Share Document