scholarly journals Verbascum Thapsus (Mullein) Versatile Polarity Extracts: GC-MS Analysis, Phytochemical Profiling, Anti-bacterial Potential and Anti-oxidant Activity

2021 ◽  
Vol 13 (6) ◽  
pp. 1488-1497
Author(s):  
Ali Nadeem ◽  
Bashir Ahmed ◽  
Hira Shahzad ◽  
Lyle E Craker ◽  
Tudor Muntean
Keyword(s):  
Verbascum Thapsus ◽  
Ms Analysis ◽  
Anti Oxidant

scholarly journals In vitro antimicrobial, anti-oxidant properties and GC-MS analysis of the crude methanolic extract and fractions of Solanum dasyphyllum Schumach and Thonn. leaves

2020 ◽  
Author(s):  
Oladapo Elijah Oyinloye ◽  
Olumuyiwa. S. Alabi ◽  
Olusegun. G. Ademowo
Keyword(s):  
Crude Extract ◽  
Skin Diseases ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Kinetics Study ◽  
Medicinal Uses ◽  
Ms Analysis ◽  
Soxhlet Apparatus ◽  
Anti Oxidant ◽  
Time Kill

Abstract Background: Solanum dasyphyllum is a plant with several ethno-medicinal uses as food and medicine in treatment of fever, skin diseases, inflammation, stomach ache and some systemic infections. To date there is little scientific validation of the folkloric claims of S. dasyphyllum compared to other species of the Solanaceae family. This study determined the antimicrobial and antioxidant potential of methanol extract and fractions of the leaves of S. dasyphyllum and analysed the phyto-constituents using GC-MS technique.Methods: The dried leaves of S. dasyphyllum were extracted by Soxhlet apparatus with 80% methanol and the crude extract subjected to Phytochemical screening and liquid-liquid fractionation. Crude extract and fractions were subjected to antimicrobial screening, minimum inhibitory concentrations (MICs) and minimum bactericidal/fungicidal concentrations (MBC/MFC) determination and time-kill kinetics study. Crude extract was assayed for antioxidant activity and analysed by GC-MS.Results: Percentage yield of S. dasyphyllum was 12.5% and tested positive for flavonoids, alkaloids, saponins, cyanogenic glycosides, tannins and reducing sugars. The crude extract and fractions were active on all the isolates at concentrations >0.5 mg/mL. MICs of the crude extract and fractions ranged between 0.25 and >4 mg/mL, MBC ranged between 1 and >4 mg/mL and MFC ranged between 0.5 and >4 mg/mL. The MBC/MIC ratio was >4 for dichloromethane fractions against S. aureus (ratio 8) and MFC/MIC ratio ethylacetate fraction against the fungi isolates (ratio 8). The time-kill kinetics study showed dichloromethane and ethylacetate fractions to be bactericidal with zero viable count at 4 - 6hours against the isolates. The crude extract displayed moderate antioxidant with weak DPPH radical scavenging activity. The GC-MS analysis showed 29 metabolites including the phenols and polyphenols derivatives, eucalyptol, levomenthol, benzofuranone derivative, diethyl phthalate, neophytadiene, 1-Docosene, 17-Pentatriacontene, Phytol, Thunbergol and esters of decanoic acid derivatives all of which are known to have anti-infective and anti-oxidant properties.Conclusion: This study therefore confirmed the ethno-medicinal claim of S. dasyphyllum and thus elucidates the potentials of the plant as a good source of bioactive compounds including those with strong anti-infective and antioxidant properties.

scholarly journals Metabolites Identification of Chemical Constituents From the Eggplant (Solanum melongena L.) Calyx in Rats by UPLC/ESI/qTOF-MS Analysis and Their Cytotoxic Activities

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuanyuan Song ◽  
Ting Mei ◽  
Yan Liu ◽  
Shengnan Kong ◽  
Jincheng Zhang ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Solanum Melongena ◽  
Rat Plasma ◽  
Cytotoxic Activities ◽  
Mcf7 Cells ◽  
Metabolic Characteristics ◽  
Ms Analysis ◽  
Incubation Experiments ◽  
Anti Oxidant

Eggplant (Solanum melongena L.) Calyx is a medicinal and edible traditional Chinese medicine with anti-inflammatory, anti-oxidant, and anti-cancer properties. However, the pharmacodynamic components and metabolic characteristics remain unclear. Amide and phenylpropanoid were the two main constituents, and four amides, including n-trans-p-coumaroyltyramine (1), n-trans-p-coumaroyloctopamine (2), n-trans-p-coumaroylnoradrenline (3), n-trans-feruloyloctopamine (4), and a phenylpropanoid neochlorogenic acid (5) were selected. In this study, these five representative compounds showed cytotoxic activities on A549, HCT116, and MCF7 cells. In addition, the metabolites of 1–5 from the eggplant calyx in rats were identified. In total, 23, 37, 29, and 17 metabolites were separately characterized in rat plasma, urine, feces, and livers, by UPLC/ESI/qTOF-MS analysis. The metabolism of amides and phenylpropanoid was mainly involved in hydroxylation, methylation, glucuronidation, or sulfation reactions. Two hydroxylated metabolites (1-M2 and 2-M3) were clearly identified by comparison with reference standards. Rat liver microsome incubation experiments indicated that P450 enzymes could hydroxylate 1–5, and the methylation reaction of the 7-hydroxyl was also observed. This is the first study on the in vivo metabolism of these compounds, which lays a foundation for follow-up studies on pharmacodynamic evaluations and mechanisms.

scholarly journals GC-MS analysis, evaluation of phytochemicals, anti-oxidant, thrombolytic and anti-inflammatory activities of Exacum bicolor

2015 ◽  
Vol 10 (4) ◽  
pp. 745 ◽  
Author(s):  
Appaji Mahesh Ashwini ◽  
Latha Puttarudrappa ◽  
Ravi Kumar ◽  
Mala Majumdar
Keyword(s):  
Methanol Extract ◽  
Clot Lysis ◽  
Total Phenolic ◽  
Dpph Assay ◽  
Thrombolytic Activity ◽  
Inflammatory Agent ◽  
Ms Analysis ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Alkaloid Contents

<p class="Abstract">The aim of the present study was to investigate the GC-MS analysis, phytochemical screening, anti-oxidant, thrombolytic and anti-inflammatory activities of methanol extract of leaves of Exacum bicolor. FTIR analysis confirmed the presence of alcohol, phenols, alkanes, aromatic compounds, aldehyde and ethers. GC-MS analysis revealed the presence of eight phyto-constituents. The total phenol, flavonoid and alkaloid contents were 18.0 ± 0.2 mg/GAE/g, 13.1 ± 0.4 mg QE/g and 108.0 ± 1.2 mg AE/g respectively. The DPPH assay exhibited potent anti-oxidant abilities with IC<sub>50 </sub>8.8 µg/mL. Significant thrombolytic activity was demonstrated by clot lysis method (45.1 ± 0.8%). The methanol extract showed significant membrane stabilization on human red blood cell with IC<sub>50 </sub>value of 37.4 µg/mL. There was a significant correlation (R<sup>2</sup>&gt;0.98) with total phenolic content versus anti-oxidant and anti-inflammatory activity. The above results confirmed that E. bicolor could be a promising anti-oxidant, thrombolytic and anti-inflammatory agent.</p><p> </p>

scholarly journals GC-MS ANALYSIS, IN-VITRO ANTI-DIABETIC AND ANTI-OXIDANT ACTIVITIES OF RHYNCHOSIA NUMMULARIA (L.) DC

2019 ◽  
Vol 10 (3) ◽  
pp. 237-241
Author(s):  
Vinoth Kumar Sekar ◽  
Gopal Venkatachalam ◽  
Devanna Nayakanti
Keyword(s):  
Ms Analysis ◽  
Anti Oxidant

scholarly journals GC-MS analysis and Molecular docking of Quercetin compounds of Phytolacca octandra with the target protein of infection causing Staphylococcus aureus

2022 ◽  
Vol 24 (1) ◽  
pp. 141-151
Author(s):  
VithyaEswari. D ◽  
◽  
R. Subashkumar ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Molecular Docking ◽  
Acyl Carrier Protein ◽  
Binding Energies ◽  
Target Protein ◽  
Gas Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Anti Oxidant ◽  
Target Binding

Phytolacca octandra is a perennial usually about 1m high herb, dense and erect in full sun. As only few reports were available on the studies about the bioactive compounds and various activities in the Phytolacca octandra, the present study focuses on the bio active compounds attributed to antibacterial activity in the plant extracts by Gas Chromatography – Mass Spectrometry (GCMS) and molecular docking methods. Antibacterial activity of Phytolacca octandra showed maximum inhibitory zones of 21mm, 18mm, 19mm, 19mm and 20mm against respective organisms for 25mg/ml of acetone extracts. The outcome of Phytolacca octandra extracts that was exposed to GC-MS analysis, showed the presence of 20 more compounds. The most identified compounds to have anti-oxidant activity are Dodecane, Octadecane and Octacosane. The other major compounds present in extract are Cyclohexen-oxopropyl, 1,2-Benzenedicarboxylic acid.The overall docking energies of the target protein, rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase with quercetin with the number of hydrogen bonds were presented in the study; The docking report revealed –8.01Kcal/Mol binding energies and 8 hydrogen bonding between the Phytolacca octandra compound, quercetin and the target binding protein, rhlG of infection causing pathogen Staphylococcus aureus.

scholarly journals Enhanced cytotoxic potential of Orthosiphon stamineus extract in MCF-7 cells through suppression of nucleolin and bcl2

2017 ◽  
Vol 12 (3) ◽  
pp. 268 ◽  
Author(s):  
Renuka Saravanan ◽  
Brindha Pemaiah ◽  
Sriram Sridharan ◽  
Mahesh Narayanan ◽  
Sivakumar Ramalingam
Keyword(s):  
Cytochrome C ◽  
Cinnamic Acid ◽  
Video Clip ◽  
Orthosiphon Stamineus ◽  
Ms Analysis ◽  
Anti Cancer ◽  
Full Screen ◽  
Anti Oxidant ◽  
Cancer Agent ◽  
Mcf 7

<p>The aim of the present study was to analyze the anti-cancer effect of <em>Orthosiphon stamineus</em> leaf extract on MCF-7 cells. Ethyl acetate extract of the plant was eluted and pooled into 6 fractions based on their R<sub>f</sub> values and the fractions were tested for cytotoxicity. Fraction 3 was found effective (IC<sub>50 </sub>= 28.5 µL/mL) and was subjected to LC-MS analysis which indicated the presence of biochanin, eleutherol and cinnamic acid. Further, cytotoxic effect of the fraction was confirmed in terms of nucleolin, bcl<sub>2</sub>, bax and cytochrome c expression in MCF-7 cells through RT-PCR where a marked decrease in nucleolin, bcl<sub>2</sub> and increase in bax and cytochrome c were observed. The presence of eleutherol, a well-known anti-oxidant and anti-cancer agent, has been reported for the first time in<em> O. stamineus</em>. The anti-cancer activity of <em>O. stamineus</em> may be due to the presence of elutherol and cinnamic acid.</p><p><strong>Video Clip of Methodology</strong>:</p><p>LC-MS analysis of <em>Orthosiphon stamineus</em> extract: 7 min 33 sec   <a href="https://youtube.com/v/7Vop4epRjB0">Full Screen</a>   <a href="https://youtube.com/watch?v=7Vop4epRjB0">Alternate</a> </p>

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