scholarly journals Enhanced cytotoxic potential of Orthosiphon stamineus extract in MCF-7 cells through suppression of nucleolin and bcl2

2017 ◽  
Vol 12 (3) ◽  
pp. 268 ◽  
Author(s):  
Renuka Saravanan ◽  
Brindha Pemaiah ◽  
Sriram Sridharan ◽  
Mahesh Narayanan ◽  
Sivakumar Ramalingam
Keyword(s):  
Cytochrome C ◽  
Cinnamic Acid ◽  
Video Clip ◽  
Orthosiphon Stamineus ◽  
Ms Analysis ◽  
Anti Cancer ◽  
Full Screen ◽  
Anti Oxidant ◽  
Cancer Agent ◽  
Mcf 7

<p>The aim of the present study was to analyze the anti-cancer effect of <em>Orthosiphon stamineus</em> leaf extract on MCF-7 cells. Ethyl acetate extract of the plant was eluted and pooled into 6 fractions based on their R<sub>f</sub> values and the fractions were tested for cytotoxicity. Fraction 3 was found effective (IC<sub>50 </sub>= 28.5 µL/mL) and was subjected to LC-MS analysis which indicated the presence of biochanin, eleutherol and cinnamic acid. Further, cytotoxic effect of the fraction was confirmed in terms of nucleolin, bcl<sub>2</sub>, bax and cytochrome c expression in MCF-7 cells through RT-PCR where a marked decrease in nucleolin, bcl<sub>2</sub> and increase in bax and cytochrome c were observed. The presence of eleutherol, a well-known anti-oxidant and anti-cancer agent, has been reported for the first time in<em> O. stamineus</em>. The anti-cancer activity of <em>O. stamineus</em> may be due to the presence of elutherol and cinnamic acid.</p><p><strong>Video Clip of Methodology</strong>:</p><p>LC-MS analysis of <em>Orthosiphon stamineus</em> extract: 7 min 33 sec   <a href="https://youtube.com/v/7Vop4epRjB0">Full Screen</a>   <a href="https://youtube.com/watch?v=7Vop4epRjB0">Alternate</a> </p>

Synthesis and Apoptotic Activities of New 2(3H)-benzoxazolone Derivatives in Breast Cancer Cells

2020 ◽  
Vol 21 (1) ◽  
pp. 84-90
Author(s):  
Emine Erdag ◽  
Eda Becer ◽  
Yusuf Mulazim ◽  
Hafize Seda Vatansever ◽  
Hilal Kabadayı ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytochrome C ◽  
Caspase 3 ◽  
Biological Activities ◽  
Cancer Cell Line ◽  
Mannich Bases ◽  
Control Group ◽  
Anti Cancer ◽  
Fas L ◽  
Mcf 7

Background: 2(3H)-Benzoxazolone derivatives are preferential structural blocks in pharmacological probe designing with the possibility of modifications at various positions on the core structure. Benzoxazolones showed various biological activities such as analgesics, anti-inflammatory and anti-cancer. Objective: In the present work, we have prepared new Mannich bases of 2(3H)-benzoxazolone derivatives and evaluated their cytotoxicities and proapoptotic properties in MCF-7 breast cancer cell line. Methods: The structures of these compounds were characterized by FT-IR, elemental analysis, 1H and 13C NMR. Cytotoxicities of all the target compounds were investigated by MTT assay. Apoptotic properties of compounds were evaluated by immunocytochemistry using antibodies against caspase-3, cytochrome-c, FasL, and also TUNEL assay. Results: These two novel compounds, 1 and 2, both have the same piperazine substituent on the nitrogen atom of benzoxazolone and the main difference in the structures of these compounds is the presence of Cl substituent at the 5- position of the benzoxazolone ring. MTT results showed that compounds 1 and 2 were effective in terms of reduction of cell viability at 100μM and 50μM concentration for 48h, respectively. As a result of immunohistochemical staining, Fas L and caspase-3 immunoreactivities were significantly increased in MCF-7 cells after treatment with compound 1. Additionally, caspase-3 and cytochrome-c immunoreactivities were also increased significantly in MCF-7 cells after treatment with compound 2. The number of TUNEL positive cells was significantly higher in MCF-7 cells when compared with the control group after treatment with both compounds 1 and 2. Conclusion: It could be concluded that N-substituted benzoxazolone derivatives increase potential anti-cancer effects and they could be promising novel therapeutic agents for chemotherapy.

Phytic acid and phosphorus in crop seeds and fruits: a global estimate

2000 ◽  
Vol 10 (1) ◽  
pp. 11-33 ◽  
Author(s):  
John N.A. Lott1 ◽  
Irene Ockenden ◽  
Victor Raboy ◽  
Graeme D. Batten
Keyword(s):  
Phytic Acid ◽  
Fruit Production ◽  
Mineral Fertilizers ◽  
Global Estimate ◽  
Anti Cancer ◽  
Potential Benefits ◽  
Anti Oxidant ◽  
Total Crop ◽  
Cancer Agent ◽  
Seeds And Fruits

AbstractA very important mineral storage compound in seeds is phytate, a mixed cation salt of phytic acid (myo-inositol hexakis phosphoric acid). This compound is important for several reasons: (1) It is vital for seed/grain development and successful seedling growth. (2) It is often considered to be an antinutritional substance in human diets, but it may have a positivenutritional role as an anti-oxidant and an anti-cancer agent. (3) It represents a very significant amount of phosphorus being extracted from soilsand subsequently removed with the crop. (4) It plays a role in eutrophication of waterways. A key part of this review is an estimate of the annualtonnage of phosphorus and phytic acid sequestered in up to 4.1 billion metric tonnes of crop seeds and fruits globally each year. We estimate thatnearly 35 million metric tonnes of phytic acid, containing 9.9 million metric tonnes of P, is combined with about 12.5 and 3.9 million metric tonnes of K and Mg respectively, to form each year over 51 million metric tonnes of phytate. The amount of P inthis phytate is equal to nearly 65÷ of the elemental P sold world wide for use in mineral fertilizers. Dry cereal grains account for 69÷ of the total crop seed/fruit production but account for 77÷ of the total phytic acid stored each year. Low phytate mutants, that are now available for some key staple food crops such as maize and barley, offer potential benefits in such areas as the sustainability of lands used to grow crops, the mineral nutrition of humans and animals, and reduction in pollution of waterways.

scholarly journals A potential estrogen receptor inhibitor compound 34 induces apoptosis via ROS-independent intrinsic apoptosis in MCF-7 cells

2019 ◽  
Vol 14 (1) ◽  
pp. 1-8
Author(s):  
Ruru Ding ◽  
Ziying Zhu ◽  
Mengting Teng ◽  
Lin Ma ◽  
Jiaying Hu ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Video Clip ◽  
Docking Studies ◽  
Intrinsic Apoptosis ◽  
Estrogen Receptor Positive ◽  
Full Screen ◽  
Apoptosis Related Protein ◽  
Induced Apoptosis ◽  
Mcf 7

This study aimed to investigate the anti-tumor effects of compound 34 on MCF-7 cells in vitro, and explore its mechanisms. MTT results showed that compound 34 selectively inhibited estrogen receptor-positive cells proliferation. Hoechst 33342 staining showed nuclear pyknosis, nuclear debris associated with apoptotic bodies. JC-1 staining showed the loss of mitochondrial membrane potential. Although compound 34 increased intracellular reactive oxygen species (ROS), compound 34-induced apoptosis was not prevented by pretreatment with ROS scavengers. Western blotting showed apoptosis-related protein like cytochrome c and cleaved PARP protein increased. Furthermore, docking studies exhibited that compound 34 could bind into ERα. In summary, compound 34 selectively inhibited estrogen receptor positive cells proliferation and induced apoptosis in MCF-7 cells via ROS-independent intrinsic apoptosis in MCF-7 cells. It may be a potential targeted drug of estrogen receptor for therapeutic application of breast cancer. Video Clip of Methodology: Assay of Cell Proliferation: 5 min 5 sec   Full Screen   Alternate  

scholarly journals Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8

2021 ◽  
Vol 12 ◽  
Author(s):  
Junrui Wu ◽  
Danli Yan ◽  
Yumeng Liu ◽  
Xue Luo ◽  
Yang Li ◽  
...  
Keyword(s):  
Leuconostoc Mesenteroides ◽  
Biological Activities ◽  
Structural Characteristics ◽  
Molecular Weights ◽  
Cycle Arrest ◽  
Glycosidic Bonds ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Inhibitory Potential ◽  
Cancer Agent

In this study, a novel exopolysaccharide (EPS) was extracted from Leuconostoc mesenteroides Shen Nong’s (SN)-8 which can be obtained from Dajiang. After the purification step, EPS-8-2 was obtained with molecular weights of 1.46 × 105 Da. The structural characterization of EPS indicated that the EPS belonged to the class polysaccharide, mainly composed of glucan and also contained certain mannose residues that were found to be connected by α-1,6 glycosidic bonds. Moreover, the results demonstrated that EPS displayed a significant capacity to scavenge free radical to some extent, and this anti-oxidant potential was found to be concentration dependent. The results further revealed that EPS displayed a significant inhibitory potential on the growth of HepG2 cells by promoting apoptosis and induced cell cycle arrest in G1 and G2 phases. Overall, these results suggested that EPS can be explored as a possible anti-cancer agent.

On the use of cytochrome C as an anti-cancer agent

2021 ◽  
Vol 1 (5) ◽  
pp. 6-13
Author(s):  
L. A. Romodin ◽  
Keyword(s):  
Lipid Peroxidation ◽  
Cell Death ◽  
Cytochrome C ◽  
Effective Means ◽  
Target Cells ◽  
Programmed Death ◽  
Anti Cancer ◽  
C Complex ◽  
Cancer Agent

As means of cancer therapy, cytochrome C can be used, which can trigger a cascade of apoptotic reactions in the cytosol, as well as its complex with cardiolipin, which triggers lipid peroxidation, which can ultimately lead to cell death by the mechanism of apoptosis or ferroptosis. This article presents the possibility of using cytochrome C and its complex with phospholipids for the treatment of cancer, as well as the main problems associated with this. The main problem is the development of an effective means of delivering a cytotoxic agent to target cells in vivo. If all problems are solved, the use of free cytochrome C should trigger apoptosis of cancer cells, and the cytochrome C complex with cardiolipin or phosphatidic acid should trigger necrosis – like programmed death by the mechanisms of necroptosis, pyroptosis or ferroptosis.

scholarly journals Effect of Artemisia persica on seizure severity and memory and learning disorders in pentylenetetrazole-kindled mice

2019 ◽  
Vol 14 (1) ◽  
pp. 36-44
Author(s):  
Mozhgan Daneshkhah ◽  
Mahbubeh Setorki
Keyword(s):  
Nitric Oxide ◽  
Memory Impairment ◽  
Video Clip ◽  
Learning Disorders ◽  
Alcoholic Extract ◽  
Hydroalcoholic Extract ◽  
Full Screen ◽  
Anti Oxidant ◽  
Seizure Model ◽  
Different Doses

The purpose of this study was to investigate the effect of hydroalcoholic extract of Artemisia persica (100, 200 and 400 mg/kg) on pentylenetetrazole-induced seizure and memory impairment. Sixty mice were assigned to seven groups. Pentylenetetrazole (35 mg/kg) was injected at 48 hours intervals for 9 days, and at 60 mg/kg on day 10 to induce seizure model. Different doses of A. persica extract were injected daily 30 min before pentylenetetrazole injection. Exposure of mice to extract significantly reduced the frequency of repeated spinning and jumping and tonic seizures in seizure model. In addition, the extract significantly reduced the increased levels of malondialdehyde in serum and brain. The extract significantly increased the serum and brain anti-oxidant capacity but had no significant effect on nitric oxide. The extract (100 mg/kg) significantly improved pentylenetetrazole-induced memory impairment. The hydro-alcoholic extract of A. persica has a protective effect against pentylenetrazole-induced seizure. Video Clip of Methodology: 1 min 56 sec: Full Screen   Alternate

scholarly journals Mammillaria Species—Polyphenols Studies and Anti-Cancer, Anti-Oxidant, and Anti-Bacterial Activities

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 131 ◽  
Author(s):  
Hosam O. Elansary ◽  
Agnieszka Szopa ◽  
Marta Klimek-Szczykutowicz ◽  
Karolina Jafernik ◽  
Halina Ekiert ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Sinapic Acid ◽  
Jurkat Cells ◽  
Gentisic Acid ◽  
Natural Product Research ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Mcf 7

Discovering new natural resources of polyphenols is the aim of many recent studies in the field of natural product research. This study tentatively investigated the polyphenols profile of the stems of seven Mammillaria species (M. rhodantha, M. spinosissima, M. hahniana, M. crucigera, M. candida, M. albilanata, and M. muehlenpfordtii) using high performance liquid chromatography with DAD detector (HPLC-DAD) method. Furthermore, the anti-cancer, anti-oxidant, and anti-bacterial potentials of these extracts as well as major identified phenols were explored. The HPLC-DAD study confirmed the availability of six phenolic acids, including gentisic acid, chlorogenic acid, caffeic acid, protocatechuic acid, sinapic acid, and p-hydroxybenzoic acid. The dominant compounds were: gentisic acid in M. rhodantha and M. spinosissima; chlorogenic acid in M. muehlenpfordtii, M. crucigera, and M. rhodantha; and caffeic acid in M. rhodantha, M. crucigera, and M. spinosissima. Stems of Mammillaria sp. showed antiproliferative effects against HeLa, MCF-7, and Jurkat cells. In HeLa and MCF-7 cells, the best antiproliferative activities were found in the treatments with M. rhodantha, M. spinosissima, and M. muehlenpfordtii. The apoptotic assay of M. rhodantha, M. spinosissima, and M. muehlenpfordtii showed accumulation of necrotic cells in the early and late apoptotic phase. M. rhodantha, M. spinosissima, and M. muehlenpfordtii showed the highest anti-oxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene bleaching, and ferric reducing anti-oxidant power (FRAP) assays. M. rhodantha was the best source of antioxidants. Mammillaria sp. showed moderate anti-bacterial effects against bacteria and the highest effects were found using the extracts of M. rhodantha, M. spinosissima, M. crucigera and M. muehlenpfordtii against most bacteria. The anti-bacterial activities were attributed to other phenolic compounds (e.g., chlorogenic acid) than gentisic acid, which was not active against most bacteria. Mammillaria sp. could be considered to be an important natural source of phenolic acids with anti-cancer, anti-bacterial, and anti-oxidant activities.

scholarly journals In vitro antifungal, anti-oxidant and HPLC analysis of the extracts of Physalis philadelphica

2017 ◽  
Vol 12 (3) ◽  
pp. 313 ◽  
Author(s):  
Wajid Khan ◽  
Jehan Bakht ◽  
Bilal Muhammad Khan
Keyword(s):  
Gallic Acid ◽  
Methanol Extract ◽  
Rhizopus Oryzae ◽  
Video Clip ◽  
Hplc Assay ◽  
Full Screen ◽  
Anti Oxidant ◽  
Extraction Solvents ◽  
Different Parts

<p class="Abstract">The different parts of<em> Physalis philadelphica</em> were evaluated for the antifungal, anti-oxidant and HPLC assay. The methanol extract of leaf showed inhibitory activity against <em>Pencillium digitatum</em> (58% ZI), <em>Aspergillus flavus</em> (95% ZI) and <em>Rhizopus oryzae</em> (96% ZI) at a concentration of 400 ppm. Strong anti-oxidant activities were found in different parts of the plant. The effects of different extraction solvents on the anti-oxidant activity and antifungal activity of the medicinal extracts were observed. The methanol extract from the leaf, stem, fruit and water fractions from the calyx exhibited strong anti-oxidant activity of 94, 89, 88 and 93% respectively. The ethyl acetate extract was found as a best solvent for the recovery of gallic acid in HPLC assay. No correlation was observed between the gallic acid and anti-oxidant capacity of the extracts.</p><p><strong>Video Clip of Methodology</strong>:</p><p>11 min 13 sec:   <a href="https://www.youtube.com/v/WAJZoHBUzHg">Full Screen</a>   <a href="https://www.youtube.com/watch?v=WAJZoHBUzHg">Alternate</a> </p>

scholarly journals Extraction, characterization and evaluation of Eruca sativa against streptozotocin-induced diabetic nephropathy in rat

2017 ◽  
Vol 12 (2) ◽  
pp. 30 ◽  
Author(s):  
Lalit Kishore ◽  
Navpreet Kaur ◽  
Anu Kajal ◽  
Randhir Singh
Keyword(s):  
Diabetic Nephropathy ◽  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Video Clip ◽  
Elevated Serum ◽  
Serum Glucose ◽  
Eruca Sativa ◽  
Full Screen ◽  
Anti Oxidant ◽  
Significant Attenuation

<p class="Abstract">The present study was aimed to evaluate petroleum ether and hydroalcohol extracts of <em>Eruca sativa</em> seeds in the treatment of diabetic nephropathy in the rat model. GC-MS analysis of petroleum ether extract revealed the presence of fatty acids and erucic acid. Quercetin and kaempferol were isolated from the hydroalcohol extract. Diabetes was induced in rat by streptozotocin (65 mg/kg i.p.) 15 min after nicotinamide (230 mg/kg, i.p.) administration. Both the extracts at different doses produced a significant attenuation in the elevated serum glucose level. Elevated renal parameters, lipid and anti-oxidant enzyme levels were restored in a dose-dependant manner. Moreover, both extracts produced significant reduction in the formation of advanced glycation end products in the kidney. These findings suggest that <em>E. sativa</em> might inhibit the progression of diabetic nephropathy and could be a therapeutic agent for the management of diabetic nephropathy.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract">Extraction of compounds from seeds: 3 min 45 sec   <a href="https://www.youtube.com/v/COwp_zrok24">Full Screen</a>   <a href="https://www.youtube.com/watch?v=COwp_zrok24">Alternate</a></p>

Sign in / Sign up

Export Citation Format

Share Document