scholarly journals Effects of Trichoderma asperellum BV10 and Bacillus amyloliquefaciens BV03 in Meloidogyne incognita Control Considering Three Different Management Systems

2022 ◽  
Vol 14 (2) ◽  
pp. 48
Author(s):  
Camila Rebelatto Muniz ◽  
Jéssica Brasau da Silva ◽  
Caroline Sayuri Nishisaka ◽  
Josiane Barros Chiaramonte ◽  
Veridiana Cardozo Gonçalves Cantão ◽  
...  

Crop yield decrease is the main concern when a pathogen or plague is identified in an agriculture field. Thus, part of this issue can be attributed to plant-parasitic nematodes (PPNs), such as Meloidogyne species, due to, most of the time, the hard diagnosis, and non-specific symptoms. Its management is mainly based on chemical pesticides, followed by a few potential biological control agents, and the management system. Therefore, this study aimed to evaluate the effects of biological agents in Meloidogyne incognita control in different soil systems. For that, two biological products were chosen, Trichoderma asperellum BV10 and Bacillus amyloliquefaciens BV03, and soils were sampled from three different managements systems: (i) soybean no-tilled system at Goiás state, Brazil; (ii) forest soil at Goiás state, Brazil, and (iii) soybean conventional managed system at Mato Grosso do Sul state, Brazil. Biocontrol and growth promotion effects, volatile organic compounds (VOCs) and soil respiration were determined in vegetation house and laboratory, respectively. As a result, both BV10 and BV03 had significant nematode control activity, comparing to control treatment, in all the three soils systems. Plus, the number of immobile nematodes by potential VOCs had significant increase when BV03 was applied, while the application of BV10 agent raised the soil respiration rate. In conclusion, both biocontrol agents presented great efficiency in control M. incognita, with better performance of BV03. Lastly, more studies must be done to elucidate how the resident soil microbiome can influence on biocontrol agent establishment and performance, as well as the consequence of the application of biological products on soil microbiome network.

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Valliappan Karuppiah ◽  
Lu Zhixiang ◽  
Hongyi Liu ◽  
Murugappan Vallikkannu ◽  
Jie Chen

Abstract Background Retention of agricultural bio-mass residues without proper treatment could affect the subsequent plant growth. In the present investigation, the co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens has been employed for multiple benefits including the enrichment of lignocellulose biodegradation, plant growth, defense potential and disease resistance. Results The Vel1 gene predominantly regulates the secondary metabolites, sexual and asexual development as well as cellulases and polysaccharide hydrolases productions. Overexpression mutant of the Trichoderma asperellum Vel1 locus (TA OE-Vel1) enhanced the activity of FPAase, CMCase, PNPCase, PNPGase, xylanase I, and xylanase II through the regulation of transcription regulating factors and the activation of cellulase and xylanase encoding genes. Further, these genes were induced upon co-cultivation with Bacillus amyloliquefaciens (BA). The co-culture of TA OE-Vel1 + BA produced the best composition of enzymes and the highest biomass hydrolysis yield of 89.56 ± 0.61%. The co-culture of TA OE-Vel1 + BA increased the corn stover degradation by the secretion of cellulolytic enzymes and maintained the C/N ratio of the corn stover amended soil. Moreover, the TA OE-Vel1 + BA increased the maize plant growth, expression of defense gene and disease resistance against Fusarium verticillioides and Cohilohorus herostrophus. Conclusion The co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens could be utilized as a profound and meaningful technique for the retention of agro residues and subsequent plant growth.


Author(s):  

Abstract A new distribution map is provided for Meloidogyne incognita (Kofoid & White) Chitwood Nematoda: Meloidogynidae Polyphagous. Information is given on the geographical distribution in EUROPE, Albania, Belarus, Belgium, Bosnia-Herzegovina, Bulgaria, Cyprus, Estonia, France, Germany, Greece, Hungary, Iceland, Italy, Latvia, Lithuania, Macedonia, Malta, Moldova, Netherlands, Poland, Portugal, Romania, Central Russia Russian Far East, Northern Russia, Southern Russia, Western Siberia, Spain, Canary, Islands Mainland Spain, Switzerland, UK, Ukraine, Yugoslavia (Fed. Rep.), ASIA, Armenia, Bangladesh, Brunei Darussalam, China, Anhui, Fujian, Guangdong, Guangxi, Guizhou, Hainan, Hebei, Heilongjiang, Henan, Hubei, Hunan, Jiangsu, Jiangxi, Nei, Menggu, Qinghai, Shaanxi, Shandong, Sichuan, Yunnan, Zhejiang, Republic of Georgia, India, Andaman and Nicobar Islands, Andhra Pradesh, Arunachal Pradesh, Assam, Bihar, Chandigarh, Delhi, Gujarat, Haryana, Himachal Pradesh, Jammu and Kashmir, Karnataka, Kerala, Madhya Pradesh, Maharashtra, Manipur, Meghalaya, Orissa, Punjab, Rajasthan, Sikkim, Tamil Nadu, Tripura, Uttar Pradesh, West Bengal, Indonesia, Java, Sumatra, Iran, Iraq, Israel, Japan, Hokkaido, Honshu, Kyushu, Ryukyu Archipelago, Shikoku, Jordan, Kazakhstan, Korea, Republic, Lebanon, Malaysia, Peninsular Malaysia, Sabah, Sarawak, Mongolia, Myanmar, Nepal, Oman, Pakistan, Philippines, Saudi Arabia, Singapore, Sri Lanka, Syria, Taiwan, Tajikistan, Thailand, Turkey, Turkmenistan, Uzbekistan, Vietnam, Yemen, AFRICA, Algeria, Angola, Burkina Faso, Cameroon, Congo, Democratic Republic, Cote d'Ivoire, Egypt, Ethiopia, Gambia, Ghana, Guinea, Kenya, Liberia, Libya, Madagascar, Malawi, Mauritania, Mauritius, Morocco, Mozambique, Niger, Nigeria, Reunion Senegal, Seychelles, Somalia, South Africa, Sudan, Tanzania, Tunisia, Uganda, Zambia, Zimbabwe, NORTH AMERICA, Canada, Ontario, Quebec, Mexico, USA, Alabama, Arizona, Arkansas, California, Connecticut, Florida, Georgia, Hawaii, Illinois, Indiana, Kansas, Kentucky, Louisiana, Maryland, Mississippi, Missouri, New Mexico, New York, North Carolina, Oklahoma, Oregon, Pennsylvania, South Carolina, Tennessee, Texas, Utah, Virginia, Washington, West Virginia, Dominica, Dominican Republic, El Salvador, Guadeloupe, Guatemala, Haiti, Honduras, Jamaica, Martinique, Montserrat, Nicaragua, Panama, Puerto Rico, St Lucia, St Vincent and Grenadines, Trinidad and Tobago, SOUTH AMERICA, Argentina, Bolivia, Brazil, Bahia, Ceara, Espirito, Santo, Goias, Maranhao, Mato, Grosso, do Sul, Minas Gerais, Para, CENTRAL AMERICA & CARIBBEAN, Antigua and Barbuda, Barbados, Belize, Bermuda, Costa Rica, Cuba, Paraiba, Parana, Pernambuco, Rio de Janeiro, Rio Grande do Norte, Rio Grande do Sul, Santa, Catarina, Sao Paulo, Chile, Colombia, Ecuador, French, Guiana, Guyana, Paraguay, Peru, Suriname, Uruguay, Venezuela, OCEANIA, American, Samoa, Australia, New South Wales, Northern Territory, Queensland, South Australia, Tasmania, Victoria, Western Australia, Fiji, Kiribati, New Caledonia, New Zealand, Niue, Norfolk Island, Papua New Guinea, Samoa, Solomon Islands, Tonga, Tuvalu, Vanuatu.


Plant Disease ◽  
1997 ◽  
Vol 81 (6) ◽  
pp. 607-613 ◽  
Author(s):  
M. P. Ko ◽  
D. P. Schmitt ◽  
M. Saxby

The influence of container bases on nematode spread from infested pots or ground in an ornamental foliage nursery was investigated with Meloidogyne incognita as the test nematode and cowpea as the indicator plant. The container bases examined were black cloth, concrete masonry blocks, gravel, crushed cinders, and bare ground. Masonry blocks raised 46 cm above ground (raised bench) served as the control treatment. Under the standard nursery practice of irrigation by rainfall and supplemental overhead sprinkler, nematodes spread from contaminated pots or ground to adjacent nematode-free pots on all tested container bases. Incidence of spread, measured as the percentage of nematode-free pots that became contaminated, increased over time and occurred more extensively from ground to pot than from pot to pot. Ground to pot spread increased rapidly following heavy rains, indicating rain was an important contributing factor. On the M. incognita-infested nursery ground, the nematode was found more frequently associated with weeds than with bare soil, suggesting that weeds were important reservoirs of nematode inoculum for rain-splash dispersal. Placement of containers on a raised bench was more effective than on any of the unraised container bases in preventing the ground to pot spread.


Author(s):  
Muhammad Naveed ◽  
Syeda Sosan Bukhari ◽  
Adnan Mustafa ◽  
Allah Ditta ◽  
Saud Alamri ◽  
...  

Nickel (Ni) bioavailable fraction in the soil is of utmost importance because of its involvement in plant growth and environmental feedbacks. High concentrations of Ni in the soil environment, especially in the root zone, may retard plant growth that ultimately results in reduced plant biomass and yield. However, endophytic microorganisms have great potential to reduce the toxicity of Ni, especially when applied together with zeolite. The present research work was conducted to evaluate the potential effects of an endophytic bacterium Caulobacter sp. MN13 in combination with zeolite on the physiology, growth, quality, and yield of sesame plant under normal and Ni stressed soil conditions through possible reduction of Ni uptake. Surface sterilized sesame seeds were sown in pots filled with artificially Ni contaminated soil amended with zeolite. Results revealed that plant agronomic attributes such as shoot root dry weight, total number of pods, and 1000-grains weight were increased by 41, 45, 54, and 65%, respectively, over control treatment, with combined application of bacteria and zeolite in Ni contaminated soil. In comparison to control, the gaseous exchange parameters (CO2 assimilation rate, transpiration rate, stomatal- sub-stomatal conductance, chlorophyll content, and vapor pressure) were significantly enhanced by co-application of bacteria and zeolite ranging from 20 to 49% under Ni stress. Moreover, the combined utilization of bacteria and zeolite considerably improved water relations of sesame plant, in terms of relative water content (RWC) and relative membrane permeability (RMP) along with improvement in biochemical components (protein, ash, crude fiber, fat), and micronutrients in normal as well as in Ni contaminated soil. Moreover, the same treatment modulated the Ni-stress in plants through improvement in antioxidant enzymes (AEs) activities along with improved Ni concentration in the soil and different plant tissues. Correlation and principal component analysis (PCA) further revealed that combined application of metal-tolerant bacterium Caulobacter sp. MN13 and zeolite is the most influential strategy in alleviating Ni-induced stress and subsequent improvement in growth, yield, and physio-biochemical attributes of sesame plant.


2015 ◽  
Vol 195 ◽  
pp. 8-16 ◽  
Author(s):  
José Gilberto Ortega-García ◽  
Roberto Montes-Belmont ◽  
Mario Rodríguez-Monroy ◽  
José Augusto Ramírez-Trujillo ◽  
Ramón Suárez-Rodríguez ◽  
...  

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 768 ◽  
Author(s):  
Yile Zhai ◽  
Zongze Shao ◽  
Minmin Cai ◽  
Longyu Zheng ◽  
Guangyu Li ◽  
...  

Pseudomonas putida MCCC 1A00316 was originally isolated from an Antarctic soil and has demonstrated potential nematicidal activity. Thus, it has promising applications for the biological control of Meloidogyne incognita. The larval mortality and egg-hatching inhibition rates of M. incognita will increase with the rising concentration of culture filtrates of P. putida MCCC 1A00316 and the duration of exposure. Thus, this study aimed to separate, purify, and identify nematicidal compounds from P. putida MCCC 1A00316 and to validate their anti-M. incognita activities. Compounds were purified through silica gel column chromatography and thin-layer chromatography combined with high-performance liquid chromatography (HPLC). Structural identification was conducted through liquid chromatography time-of-flight mass spectrometry, 1H nuclear magnetic resonance (NMR) spectroscopy, 13C-NMR, and Marfey’s method. The isolated compounds were identified as cyclo(l-Pro–l-Leu) on the basis of the results of the above analyses and previously reported data. The effects of various concentrations of cyclo(l-Pro–l-Leu) on the mortality rates of second-stage juveniles (J2) of M. incognita were investigated. Results showed that HPLC-purified cyclo(l-Pro–l-Leu) displayed nematicidal activities. The mortality rate of M. incognita J2 reached 84.3% after 72 h of exposure to 67.5 mg/L cyclo(l-Pro–l-Leu). The lowest egg-hatching rate (9.74%) was observed after 8 days of incubation with 2000 mg/L cyclo(l-Pro–l-Leu). An egg-hatching rate of 53.11% was obtained under the control treatment (sterile distilled water). However, cyclo(l-Pro–l-Leu) did not elicit chemotaxis activity to M. incognita. This is the first work to investigate the anti-M. incognita characteristics of cyclo(l-Pro–l-Leu).


2016 ◽  
Vol 19 (s1) ◽  
pp. 15-17
Author(s):  
Jacek Nawrocki ◽  
Anna Pogodzińska

Abstract In two-year field experiments (2014 and 2015), the effect of used preparations on health status of leaves and roots and bulbs on two cultivars of garlic: ‘Arkus’ and ‘Garpek’ was studied. During investigations: Polyversum WP (Pythium oligandrum), Trifender WP (Trichoderma asperellum) and RhizoVital 42 (Bacillus amyloliquefaciens) and standard fungicide Topsin M 500 SC (tiophanate methyl) were used. Unprotected plants presented control. The obtained results showed that in the first year of the studies, all the tested formulations effectively protected the roots and bulbs of garlic against rot, except RhizoVital 42 for ‘Arkus’ variety and Trifender WP for cultivar ‘Garpek’. In 2015, all tested preparations, without exception, limited root rot and the rot of basal part of bulbs both cultivars of garlic. The applied biological preparations had no significant effect on health of the leaves of garlic in 2014 and in 2015 for cultivar ‘Garpek’, while in the second year of studies, all the tested formulations effectively limited the dieback of leaves of the garlic cultivar ‘Arkus’.


Author(s):  
C. Y. Shalini Udaya ◽  
S. Nakkeeran ◽  
K. Soorianathasundaram

Fusarium wilt of banana is the most devastating disease caused by Fusarium oxysporum f.sp. cubense (Foc). In order to combat the early onset of Fusarium wilt disease, an experiment was carried out on biohardening of tissue cultured plantlets. Six bacterial endophytes were observed to inhibit the growth of Foc in vitro. Among the six bacterial endophytes, Bacillus amyloliquefaciens (VB7) inhibited mycelial growth of Foc to an extent of 70.58% over control. Further, these bacterial endophytes were used for biohardening of the tissue cultured banana plantlets cv. Ney Poovan (AB) during primary and secondary hardening stages. Among the bacterial endophytes, B. amyloliquefaciens was found to significantly enhance plant height, leaf production, root numbers and root length compared to untreated control. Activity of defense enzymes were  also enhanced and such increase in activity was observed to be to an  extent of 93.67% in peroxidase, 92.39% polyphenol oxidase, 97.60% phenylalanine ammonia lyase  and 26.23% in β-1, 3-glucanase defence enzymes in plants biohardened with B. amyloliquefaciens (VB7) over untreated control after inoculation of Foc. Tissue cultured plants of Ney Poovan biohardened with B. amyloliquefaciens   (VB7),   B.   paraconglomeratum   (YEB   PT2) and S. maltophilia (YEB RH2) were completely free from wilt incidence symptoms upto planting stage when challenged with Foc inoculum under pot culture conditions. As among these three endophytes, B. amyloliquefaciens (VB7) also influenced favourable growth promotion, it can serve as a potential biocontrol agent for management of Fusarium wilt of banana.


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