scholarly journals The effect of the method of application and concentration of asahi sl on the response of cucumber plants to chilling stress

2012 ◽  
Vol 63 (2) ◽  
pp. 161-169
Author(s):  
Edward Borowski
Keyword(s):  
Chilling Stress ◽  
Growth Period ◽  
Growth Conditions ◽  
Early Growth ◽  
Proline Content ◽  
Carotenoid Content ◽  
Leaf Biomass ◽  
Fluorescent Light ◽  
Free Proline ◽  
Short Term

In pot experiments conducted on cucumber cv. Śremski F1, the effect was studied of short-term chilling stress on plants which had grown from seeds germinating in the solution of Asahi SL or treated with this biostimulator during the early growth period. The plants were grown in a phytotron at an air temperature of 27/22°C (day/night), using fluorescent light with FAR flux density of 220 &#956;mol x m<sup>-2</sup> x s<sup>-1</sup> and with a photoperiod of 16/8. The biostimulator was applied using the following methods: a) germination of seeds in 0.01% and 0.05% solution, b) watering of plants twice with 0.01% or 0.05% solution, c) spraying leaves with 0.3% or 0.5% solution. Plants sprayed with distilled water were the control. After 24 hours from foliar or root application of Asahi SL, one half of the plants from each experimental series was treated for a period of 3 days at a temperature of 12/6°C, with all the other growth conditions unchanged. The obtained results show that short-term chilling stress caused a significant increase in electrolyte leakage, free proline content and in the activity of ascorbate peroxidase in leaves, but a decrease in the content of chlorophyll, its maximum fluorescence (Fm) and quantum yield (Fv/Fm), carotenoid content, stomatal conductance, transpiration, photosynthesis, leaf biomass and in the activity of catalase in leaves. Foliar or root application of Asahi SL in the pre-stress period decreased the values of the traits which increased as a result of chilling or increased those which decreased. Higher concentrations of the biostimulator solutions, applied using this method, were more effective. The application of the biostimulator during seed germination did not result in significant changes in the response of plants to chilling stress.

scholarly journals Response to chilling in cucumber (Cucumis sativus L.) plants treated with triacontanol and Asahi SL.

2012 ◽  
Vol 62 (2) ◽  
pp. 165-172 ◽  
Author(s):  
Edward Borowski
Keyword(s):  
Chilling Stress ◽  
Growth Conditions ◽  
Proline Content ◽  
Experimental Series ◽  
The Other ◽  
Fluorescent Light ◽  
Guaiacol Peroxidase ◽  
Free Proline ◽  
Short Term ◽  
Cucumis Sativus L

In pot experiments on cucumber cv. Śremski F<sub>1</sub>, the effect of short-term chilling on plants earlier treated with triacontanol (TRIA) and Asahi SL was investigated. These plants were grown in a phytotron at an air temperature of 27/22<sup>°</sup>C (day/night), using fluorescent light with far flux density of 220 µmol × m<sup>-2</sup> × s<sup>-1</sup>, with a photoperiod 16/8. At the 4th true leaf stage, the respective experimental series were sprayed with: 1) H<sub>2</sub>O - control, 2) TRIA 0.01, 3) TRIA 0.1, 4) TRIA 1.0 mg × dm<sup>-3</sup>, 5) Asahi SL 0.2, 6) Asahi SL 0.3%. After 24 hours one half of the plants from each experimental series was treated for a period of 3 days at a temperature of 12/6<sup>°</sup>C, with all the other growth conditions unchanged. The obtained results have shown that short-term chilling stress caused a significant increase in electrolyte leakage, free proline content and in the activity of guaiacol peroxidase in leaves, but a decrease in chlorophyll a+b content, stomatal conductance, transpiration, photosynthesis, leaf area and in the activity of catalase in leaves. The application of TRIA or ASAHI SL on leaves in the pre-stress period reduced the values of the traits which had been increased as a result of chilling and increased those which had reduced. Generally, TRIA was most effective at a concentration of 0.1 mg × dm<sup>-3</sup>, and Asahi SL at a concentration of 0.3%.

Evaluation of Azadirachtin on Arthrospira plantensis Gomont growth parameters and antioxidant enzymes

2020 ◽  
Vol 56 ◽  
pp. 8
Author(s):  
Hatice Tunca ◽  
Ali Doğru ◽  
Feray Köçkar ◽  
Burçin Önem ◽  
Tuğba Ongun Sevindik
Keyword(s):  
Enzyme Activity ◽  
Growth Parameters ◽  
Arthrospira Platensis ◽  
Proline Content ◽  
Enzyme Analysis ◽  
Free Proline ◽  
Sod Activity ◽  
Pesticide Pollution ◽  
Antioxidant Parameters ◽  
Mda Content

Azadirachtin (Aza) used as insecticide due to inhibiting growth of insects and preventing them from feeding on plants. To understand the effects of contamination of this insecticide on phototrophs, and to determine the responses of these organisms against these insecticides are extremely important in understanding how the ecosystem is affected. In this study, chlorophyll-a amount, OD 560 and antioxidant parameters (total SOD, APX, GR, Proline, MDA and H2O2) were determined in order to understand the effect of Aza on Arthrospira platensis Gomont. Aza was applied between 0–20 μg mL−1 concentrations for 7 days in the study. Enzyme analysis was conducted at the end of the 7th day. There was a statistically significant decrease in the absorbance of OD560 and the chlorophyll-a content in A. platensis cultures exposed to the Aza (0–20 μg mL−1) during 7 days due to the increase in pesticide levels. SOD activity decreased at 8, 16 and 20 μg mL−1 concentrations; GR enzyme activity showed a significant decrease compared to the control at a concentration of 20 μg mL−1. APX activity did not change significantly compared to control. The MDA content increased significantly at 16 and 20 μg mL−1 concentrations. The H2O2 content significantly increased at 12, 16 and 20 μg mL−1 concentrations (p < 0.05) while the free proline content decreased at 4 μg mL−1 concentration (p < 0.05). As a result, regarding the Aza concentrations used in this study may be a step to prevent pesticide pollution in the environment.

scholarly journals Offspring telomere length in the long lived Alpine swift is negatively related to the age of their biological father and foster mother

2017 ◽  
Vol 13 (9) ◽  
pp. 20170188 ◽  
Author(s):  
François Criscuolo ◽  
Sandrine Zahn ◽  
Pierre Bize
Keyword(s):  
Parental Care ◽  
Telomere Length ◽  
Brood Size ◽  
Foster Parents ◽  
Growth Conditions ◽  
Early Growth ◽  
Foster Mother ◽  
Biological Father ◽  
Accumulation Of Damage ◽  
Older Males

A growing body of studies is showing that offspring telomere length (TL) can be influenced by the age of their parents. Such a relationship might be explained by variation in TL at conception (gamete effect) and/or by alteration of early growth conditions in species providing parental care. In a long-lived bird with bi-parental care, the Alpine swift ( Apus melba ), we exchanged an uneven number of 2 to 4-day-old nestlings between pairs as part of a brood size manipulation. Nestling TL was measured at 50 days after hatching, which allowed investigation of the influence of the age of both their biological and foster parents on offspring TL, after controlling for the manipulation. Nestling TL was negatively related to the age of their biological father and foster mother. Nestling TL did not differ between enlarged and reduced broods. These findings suggest that offspring from older males were fertilized by gametes with shorter telomeres, presumably due to a greater cell division history or a longer accumulation of damage, and that older females may have provided poorer parental care to their offspring.

scholarly journals He-Ne Laser Seed Treatment Improves the Nutraceutical Metabolic Pool of Sunflowers and Provides Better Tolerance Against Water Deficit

2021 ◽  
Vol 12 ◽  
Author(s):  
Saqib Mahmood ◽  
Beenish Afzal ◽  
Shagufta Perveen ◽  
Abdul Wahid ◽  
Muhammad Azeem ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Laser Treatment ◽  
Seed Treatment ◽  
Nutritional Value ◽  
Soluble Sugars ◽  
Growth And Yield ◽  
Oil Yield ◽  
Carotenoid Content ◽  
Leaf Biomass ◽  
Drought Tolerant

Water-scarce areas are continually increasing worldwide. This factor reduces the quantity and quality of crops produced in affected areas. Physical seed treatments are considered economical and ecofriendly solutions for such problems. It was hypothesized that a moderately drought-tolerant crop grown from seeds treated with a He-Ne laser utilizes water-limited conditions better than plants grown from untreated seeds. A field study was conducted, growing a moderately drought tolerant crop (sunflower) with supportive seed treatment (He-Ne laser treatment at 300 mJ) for 0, 1, 2, and 3 min. Thirty-day-old plants were subjected to two irrigation conditions: 100% (normal) and 50% (water stress). Harvesting was done at flowering (60-day-old plants) at full maturity. The sunflowers maintained growth and yield under water limitation with a reduced achene number. At 50%, irrigation, there was a reduction in chlorophyll a, a+b and a/b; catalase activity; soluble sugars; and anthocyanin, alongside elevated proline. The improved chlorophyll a, a+b and a/b; metabolisable energy; nutritional value; and yield in the plants grown from He-Ne-laser-treated seeds support our hypothesis. Seeds with 2-min exposure to a He-Ne laser performed best regarding leaf area; leaf number; leaf biomass; chlorophyll a, a+b and a/b; per cent oil yield; 50-achene weight; achene weight per plant; carotenoid content; and total soluble phenolic compound content. Thereafter, the leaves from the best performing level of treatment (2 min) were subjected to high-performance-liquid-chromatography-based phenolic profiling and gas-chromatography-based fatty acid profiling of the oil yield. The He-Ne laser treatment led to the accumulation of nutraceutical phenolic compounds and improved the unsaturated-to-saturated fatty acid ratio of the oil. In conclusion, 2-min He-Ne laser seed treatment could be the best strategy to improve the yield and nutritional value of sunflowers grown in water-limited areas.

scholarly journals Integrated effects of poultry manure and chemical fertilizer on the growth, leaf yield and stevioside content of stevia

2016 ◽  
Vol 13 (2) ◽  
pp. 175-182 ◽  
Author(s):  
MM Zaman ◽  
MAH Chowdhury ◽  
Tanzin Chowdhury
Keyword(s):  
Calcareous Soil ◽  
Biomass Yield ◽  
Acid Soil ◽  
Poultry Manure ◽  
Growth Period ◽  
Chemical Fertilizer ◽  
Leaf Biomass ◽  
Agricultural Chemistry ◽  
Leaf Yield ◽  
Overall Performance

Stevia is important for its leaves which are used as non calorie sweetener and also in many therapeutic applications including diabetes. The present investigation was carried out in the net house of the Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh to investigate the integrated effects of poultry manure (PM) and chemical fertilizer (CF) on the growth, leaf biomass yield and stevioside content of stevia grown in two contrasting soils (acid and non-calcareous) of Bangladesh. Six combinations of PM and CF viz. PM0+CF0, PM0+CF100, PM3+CF50, PM3+CF75, PM5+CF50, PM5+CF75 were used. The study revealed that PM along with different percentages of chemical fertilizers exerted significant influence on the growth, leaf yield and stevioside content of stevia. The highest values of all the parameters except stevioside were found from the plant receiving PM @ 3 t ha-1 along with 75% CF. The leaf biomass yield was 1088% and 867% higher in acid soil and non-calcareous soil, respectively, over control. Stevioside content was increased with increasing the levels of PM up to 5 t ha-1 and also with the advancement of growth period from 30 to 60 days after planting. The highest amount of stevioside (10.45%) was detected in the leaves of the plant that received PM @ 5 t ha-1 along with 75% CF at 60 DAP in acid soil and it was 9.11% in non-calcareous soil at the same period. The lowest values of all the parameters including stevioside content were obtained from the plant which received neither PM nor CF. Considering the overall performance, farmers may be advised to cultivate stevia in acid soil applying PM @ 3 t ha-1 along with 75% chemical fertilizer to boost up the production of stevia under the agro-climatic condition of the study area in the context of Bangladesh.J. Bangladesh Agril. Univ. 13(2): 175-182, December 2015

Quality and Intensity of Light in the In Vitro Development of Microstumps of Eucalyptus urophylla in a Photoautotrophic System

2020 ◽  
Vol 66 (6) ◽  
pp. 754-760 ◽  
Author(s):  
Natane A Miranda ◽  
Aloisio Xavier ◽  
Wagner C Otoni ◽  
Ricardo Gallo ◽  
Kellen C Gatti ◽  
...  
Keyword(s):  
Plant Growth ◽  
Light Quality ◽  
Stomatal Density ◽  
Carotenoid Content ◽  
Fluorescent Light ◽  
Eucalyptus Urophylla ◽  
Light Emitting ◽  
Fluorescent Lamps ◽  
Number Of Shoots

Abstract The quality and quantity of light are important factors in controlling in vitro plant growth in photoautotrophic systems. The aim of this study was to evaluate the influence of light quality (fluorescent, white, red, blue, red/blue, and distant red) on microstumps of a Eucalyptus urophylla clone in an in vitro photoautotrophic system, as well as the intensity of fluorescent light (60, 85, 100, and 140 μmol m–2 s–1) in the growth and production of microcutting. The number of shoots and microcutting, the size of the largest shoot, the stomatal density, chlorophyll, and carotenoid content were analyzed. Light quality altered plant growth, and fluorescent light intensity did not affect the microstumps’ production during the evaluation period. In white light-emitting diode (LED) light, there was higher production of carotenoids, with a lower initial production of microcuttings. A smaller number of shoots were obtained in blue LED. In general, the different qualities and light intensities tested allowed for the growth of the Eucalyptus urophylla clone grown in vitro, making it possible to obtain microcuttings under photoautotrophic cultivation. Study Implications In vitro propagation is a stressful process for plants and has limitations for commercial-scale Eucalyptus production. Fluorescent lamps, closed containers, and high sucrose concentrations are traditionally used. To reduce costs and improve production, the use of efficient light sources and photoautotrophic cultivation systems become alternatives. This study investigated the influence of light on the in vitro growth of a Eucalyptus clone in a photoautotrophic system. The quality was more important than the intensity of light. Foresters will be able to indicate the use of LEDs (light-emitting diodes) as a replacement for fluorescent lamps. This approach is useful in enhancing micropropagation techniques.

Growth and Free Proline Content of Tobacco Callus and HeLa Cells Exposed in Vitro to Rubber Dust and Carbon Black

AIHAJ ◽  
1969 ◽  
Vol 30 (4) ◽  
pp. 402-406 ◽  
Author(s):  
Carroll W. Smith ◽  
Simon H. Wender ◽  
Carl A. Nau
Keyword(s):  
Carbon Black ◽  
Hela Cells ◽  
Proline Content ◽  
Tobacco Callus ◽  
Free Proline

Irrigation of kenaf (Hibiscus cannabinus L.) in the Sudan

1981 ◽  
Vol 96 (3) ◽  
pp. 569-578
Author(s):  
S. M. Farah
Keyword(s):  
Previous Experiment ◽  
Growth Period ◽  
Early Growth ◽  
Hibiscus Cannabinus ◽  
The Other ◽  
Stages Of Growth ◽  
Early Maturing ◽  
Late Stages ◽  
Infiltration Method ◽  
Late Growth

SUMMARYIn one experiment during 1972 and 1973 five irrigation regimes of every 4, 6, 8 and 10 days and according to stomatal opening, as estimated by the infiltration method, were compared, using an early-maturing variety Zankawa and a late-maturing variety G51. In both experiments early crop growth was checked by the shortest and the longest irrigation intervals.Late growth, on the other hand, was promoted by the shortest intervals in both seasons. Thus the highest yield in 1972 was obtained from the 4–day regime, followed by the infiltration method, then progressively greater yields with shorter intervals. In 1973, however, the shortest interval resulted in the lowest yield, which was significantly less than the other treatments, which showed a similar trend to those of 1972.In a second experiment during 1974 and 1975 a medium-maturing variety 22/9/1 and a late–maturing variety 44/E were added to those of the previous experiment. Watering every 4 and 6 days were eliminated and watering every 12 days added, during the early growth period. Each of these regimes was given irrigation every 6, 8, 10 and 12 days during the late growth period. The early-maturing varieties yielded best when irrigated every 12 days in the early stages, and every 10 days in the late stages, whereas the late-maturing varieties yielded best when irrigated every 10–2 and 8 days in the early and late stages of growth, respectively.

THE METABOLISM OF YEAST SPORULATION: VI. CHANGES IN AMINO ACID CONTENT DURING SPOROGENESIS

1964 ◽  
Vol 10 (5) ◽  
pp. 623-631 ◽  
Author(s):  
C. Ramirez ◽  
J. J. Miller
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Content ◽  
Proline Content ◽  
Sporulation Medium ◽  
Free Proline

During 6-day exposures of cells of Saccharomyces cerevisiae to acetate sporulation medium, the content of free amino acids declined to approximately one-third of that of vegetative cells, but proline was exceptional in that it increased conspicuously in amount. The content of combined amino acids also diminished to about one-third, ammonia was evolved, and amino acids (not including proline) passed out of the cells into the medium. When dihydroxyacetone replaced acetate in the sporulation medium, the results were similar except that the decline in content of free and combined amino acids was much greater, more ammonia was evolved, and only very small amounts of amino acids could be detected in the medium. Transfer of sporulated cells to growth medium led to an increase in the pool of free amino acids, except for proline, which declined in amount.In two other species of Saccharomyces the free proline content also increased on exposure to sporulation medium, but in Schizosaccharomyces pombe and Torulopsis famata no such increase was observed.

Sign in / Sign up

Export Citation Format

Share Document