scholarly journals Observation of sperm-head vacuoles and sperm morphology under light microscope

2014 ◽  
Vol 41 (3) ◽  
pp. 132 ◽  
Author(s):  
Yong-Seog Park ◽  
Sol Park ◽  
Duck Sung Ko ◽  
Dong Wook Park ◽  
Ju Tae Seo ◽  
...  
Keyword(s):  
Light Microscope ◽  
Sperm Morphology ◽  
Sperm Head

scholarly journals Assessment of Sperm Quality - A Light Microscope Study

2021 ◽  
Vol 10 (19) ◽  
pp. 1417-1421
Author(s):  
Jyothi A. Raj ◽  
Heera Sankar ◽  
Sagarika Mahapatra ◽  
Ashima Binny
Keyword(s):  
Light Microscope ◽  
Sperm Morphology ◽  
Clinical Laboratory ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Complete Evaluation ◽  
Normal Sperm ◽  
Middle Piece ◽  
Sperm Counts ◽  
Work Up

BACKGROUND Semen analysis is an integral part of work up for infertility in men, with sperm morphology being an important qualitative parameter. Qualitative defects can affect any part of the sperm and are classified as defects in the head, middle piece, and tail, based on morphology. The focus of the study was to assess qualitative defects in sperms by light microscopy, in semen with normal sperm counts. METHODS This study is hospital based, descriptive, retrospective study. Of the semen samples received in the clinical laboratory, fifty with normal sperm counts were included in the study and processed according to standard protocol. For evaluation of qualitative defects by sperm morphology, smears were fixed in ethanol, stained with Papanicolaou stain [PAP], and assessed under light microscope. RESULTS The 50 semen samples included in the study had sperm counts ranging from 15 to 80 million / ml. Thirty samples had less than 10 % abnormal forms, fourteen samples had 11 - 20 % abnormal forms, five samples had 21 - 30 % abnormal forms and one sample had 40 % abnormal sperms. Qualitative defects were classified as morphological abnormalities in head, neck, and tail. Of the fifty cases, most defects were found in the head, followed by those in the neck and tail. Common defects noted were double head (44 %), abnormal sized heads, and bent neck (48 %). Coiling was a common defect noted in the tail (10 %). Most sperms showed a combination of defects. CONCLUSIONS Qualitative defects in sperm morphology are often seen in samples with normal sperm counts. Assessment of microscopic characteristics of human spermatozoa is as important as count and motility in the complete evaluation and work-up of semen samples in cases of infertility. KEY WORDS Semen, Sperm, Quality, Microscopy, Morphology

Sperm morphology of the Rattini – are the interspecific differences due to variation in intensity of intermale sperm competition?

2018 ◽  
Vol 30 (11) ◽  
pp. 1434 ◽  
Author(s):  
Tessa Pahl ◽  
Hanna J. McLennan ◽  
Yun Wang ◽  
Anang S. Achmadi ◽  
Kevin C. Rowe ◽  
...  
Keyword(s):  
Sexual Selection ◽  
Sperm Competition ◽  
Sperm Morphology ◽  
Tail Length ◽  
Sperm Head ◽  
Apical Hook ◽  
Interspecific Differences ◽  
Murine Rodent ◽  
Murine Rodents ◽  
Sperm Size

It is widely accepted that in mammals a causal relationship exists between postcopulatory sexual selection and relative testes mass of the species concerned, but how much it determines sperm size and shape is debatable. Here we detailed for the largest murine rodent tribe, the Rattini, the interspecific differences in relative testes mass and sperm form. We found that residual testes mass correlates with sperm head apical hook length as well as its angle, together with tail length, and that within several lineages a few species have evolved highly divergent sperm morphology with a reduced or absent apical hook and shorter tail. Although most species have a relative testes mass of 1–4%, these derived sperm traits invariably co-occur in species with much smaller relative testes mass. We therefore suggest that high levels of intermale sperm competition maintain a sperm head with a long apical hook and long tail, whereas low levels of intermale sperm competition generally result in divergent sperm heads with a short or non-existent apical hook and shorter tail. We thus conclude that sexual selection is a major selective force in driving sperm head form and tail length in this large tribe of murine rodents.

Effect of management systems and seasons on sperm abnormalities in Jamunapari bucks semen

2017 ◽  
Author(s):  
N. Ramachandran ◽  
N. P. Singh
Keyword(s):  
Analysis Of Variance ◽  
Sperm Morphology ◽  
Winter Season ◽  
Sperm Head ◽  
Management Systems ◽  
Sperm Abnormalities ◽  
The Mean ◽  
One Year

The sperm morphology of Jamunapari bucks reared under stall feeding (SF) and grazing cum supplementation (GS) systems was studied for continuous one year. The overall LSM for the head, midpiece, tail and total abnormal sperms were 0.95, 0.09, 0.41 and 1.97%, irrespective of management systems and seasons. The sperm head, midpiece, tail and total abnormality was significantly (P less than 0.01) higher in semen of GS bucks as compared to SF bucks. The mean head abnormal sperms irrespective of management systems were 0.71, 0.73 and 1.50 % in rainy, winter and summer seasons, respectively. The respective values for midpiece, tail and total abnormality were observed to be 0.24, 0.04, 0.05%; 0.67, 0.32, 0.30% and 2.05, 1.53, 2.38%. Analysis of variance indicated that all the sperm abnormalities varied significantly (P less than 0.01) among seasons. The results suggest that stall feeding and winter season is superior for harvesting spermatozoa with lower sperm abnormalities than other seasons and GS systems.

Scanning electron microscopic studies on spermatozoa of anurans from India and Sri Lanka

2001 ◽  
Vol 22 (3) ◽  
pp. 303-308 ◽  
Author(s):  
Hareesh Joshy ◽  
Mitsuru Kuramoto
Keyword(s):  
Sri Lanka ◽  
Sperm Morphology ◽  
Sperm Head ◽  
Electron Microscopic ◽  
Frog Species ◽  
Scanning Electron Microscopic ◽  
Microscopic Studies ◽  
Generalized Type ◽  
Scanning Electron ◽  
Shape And Size

AbstractThe shape and size of spermatozoa of 11 frog species from India and Sri Lanka were examined by scanning electron microscopy. The spermatozoa of the genera Limnonectes and Euphlyctis were of the generalized type with a thick sperm head and a thin tail, whereas Indirana semipalmata had peculiar spermatozoa with a densely coiled sperm head and a thick tail. Rhacophorus microtympanum is likely to belong to the genus Philautus from sperm morphology. The spermatozoa of Microhyla ornata and Ramanella obscura were very similar, with a cone-shaped sperm head and a thin tail.

scholarly journals Morphometric dimensions of the stallion sperm head depending on the staining method used

2015 ◽  
Vol 59 (2) ◽  
pp. 263-270 ◽  
Author(s):  
Dorota Banaszewska ◽  
Magdalena Czubaszek ◽  
Renata Walczak-Jędrzejowska ◽  
Katarzyna Andraszek
Keyword(s):  
Surface Area ◽  
Sperm Morphology ◽  
Staining Method ◽  
Sperm Head ◽  
Morphometric Measurements ◽  
Morphological Evaluation ◽  
Length Width ◽  
Staining Techniques ◽  
Control Samples

AbstractSemen was collected from Polish Halfbred stallions. Twenty individuals from 3 to 4 years of age were selected for the study. At least one ejaculate from each stallion was collected and assessed. Sperm morphology was evaluated using Papanicolaou stain, SpermBlue®, and a complex of eosin and gentian stain, whereas unstained slides were used as control samples. Morphometric measurements were performed on 100 randomly selected sperm heads in each ejaculate. The length, width, perimeter, and surface area of the sperm head were measured. The frequency of nuclear vacuoles was determined as well. Tygerberg’s strict criteria, which most precisely characterise the sperm head, were used in the morphological evaluation of the sperm. The results obtained indicate that in the case of staining with SpermBlue®and with eosin+gentian complex, the morphometry of the sperm head seems to be most similar to that observed in unstained smears. It also seems that neither shrinking nor swelling is uniform in the various staining techniques (Papanicolaou stain). Moreover, it appears that in comparison with unstained sperm, all methods caused the width of the head to increase as a result of swelling.

XVIII.—The Spermatozoon of the Spider BeetlePtinus tectusBoieldieu as seen by Light and Electron Microscopy

1951 ◽  
Vol 64 (3) ◽  
pp. 353-366 ◽  
Author(s):  
Jozef Dlugosz ◽  
John W. Harrold
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Light Microscope ◽  
Light And Electron Microscopy ◽  
Sperm Head ◽  
Central Axis

SynopsisThe mature Ptinid sperm examined under the light microscope is found to be specialised in that the chromatin is not contained within a sperm head but is distributed along a central axis. The migration of chromatin resembles that found in Coccids by Hughes-Schrader (1948). Surrounding the axis is a more flexible helical membrane extending the whole length of the sperm.Under the electron microscope the membrane appears to consist of eighteen or twenty thin fibres and two thick fibres with striated sheaths. Near the posterior end of the membrane the fibres are surrounded by a ring. The structure is simpler than that of mammalian and avian sperms examined by other workers with similar techniques. Under the electron microscope, stages in the migration of chromatin in the immature sperm show a number of discrete opaque bodies which may be chromosomes. The approximate dimensions of the various structures are given.

Sperm morphology, motility and fertilisation capacity in the myobatrachid frog Crinia georgiana

2010 ◽  
Vol 22 (3) ◽  
pp. 516 ◽  
Author(s):  
Martin A. Dziminski ◽  
J. D. Roberts ◽  
Leigh W. Simmons
Keyword(s):  
Male Fertility ◽  
Sperm Morphology ◽  
Negative Relationship ◽  
Tail Length ◽  
Aquatic Organisms ◽  
Sperm Head ◽  
Swimming Velocity ◽  
Male Body ◽  
Sperm Viability ◽  
Sperm Traits

Sperm traits have been found to vary between individuals within populations in a variety of taxa. Sperm motility, morphometry and viability may be expected to have important effects on male fertility, although previous studies have found varying patterns, especially in external fertilisers. In the present study, we examined the effects of sperm swimming velocity, the proportion of motile spermatozoa, sperm head and tail length and the proportion of live spermatozoa on fertilisation success in the externally fertilising myobatrachid frog Crinia georgiana using IVF techniques and by controlling sperm numbers. We found no effect of any of the sperm traits we measured on IVF success. Neither did we find any relationship between sperm morphology and sperm performance. There was a negative relationship between sperm viability and male body size, which could be a function of age or an alternative tactic of differential investment in spermatozoa by smaller-sized males using sneak tactics in multiple matings. In contrast with most externally fertilising aquatic organisms, high rates of fertilisation appear to be achieved in C. georgiana with relatively low sperm swimming speeds.

scholarly journals Assessment of the morphometry of heads of normal sperm and sperm with the Dag defect in the semen of Duroc boars

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Dorota Banaszewska ◽  
Katarzyna Andraszek
Keyword(s):  
Surface Area ◽  
Sperm Morphology ◽  
Morphological Structure ◽  
Sperm Head ◽  
Morphometric Parameters ◽  
Livestock Species ◽  
Normal Sperm ◽  
Shape Indices ◽  
Morphological Defects ◽  
The Individual

Abstract Introduction The Dag defect is one of the primary morphological defects in sperm correlating with reduced fertility. This defect is found in the spermatozoa of many livestock species. The aim of the study was to assess the morphometry of the heads of normal sperm and sperm with the Dag defect in the semen of Duroc breeding boars. Material and Methods Sperm morphology was examined in ten ejaculates each from 12 Duroc boars. In total, 3,600 morphologically normal sperm and 838 sperm with the Dag defect were evaluated. The area, perimeter, length and width of the sperm head were measured and these basic morphometric parameters were used to calculate four additional shape indices characterising the sperm head, i.e. ellipticity, elongation, roughness and regularity. Results Sperm with this defect had markedly smaller heads, 0.32 μm shorter and 0.19 μm narrower than the heads of sperm with normal morphological structure. The heads of sperm with the Dag defect also had a 1.1μm smaller perimeter and a 2.5 μm2 smaller surface area than the heads of morphologically normal sperm. Conclusions The Dag defect is found in boar sperm irrespective of the age of the individual. It affects the morphology of the sperm head.

222. Sperm morphology within the testis and cauda epididymis of a Tasmanian devil (Sarcophilus harrisii)

2005 ◽  
Vol 17 (9) ◽  
pp. 86 ◽  
Author(s):  
P. L. Matson ◽  
P. A. Eden ◽  
S. D. Vitali ◽  
C. A. Lawrence ◽  
H. M. Robertson
Keyword(s):  
Sperm Morphology ◽  
Acute Angle ◽  
Sperm Head ◽  
Tasmanian Devil ◽  
Optimum Conditions ◽  
Testicular Sperm ◽  
Stages Of Development ◽  
Straight Line ◽  
Epididymal Maturation ◽  
Significant Difference

A male Tasmanian devil, aged 6.5 years, was euthanased with intravenous pentobarbitone overdose due to the diagnosis of squamous cell carcinoma. The testes and cauda epididymes were removed immediately after euthanasia and placed into TALP-HEPES medium containing 3% BSA and dissected. Smears of the isolated sperm were made, air-dried and stained using Diff-Quik. Sperm were classified using a modification of a system originally applied to the brush-tailed possum1 as follows: (a) Immature I – head is perpendicular or greater than 45° to the tail.(b) Immature II – head forms an acute angle of 45° or less with the tail.(c) Immature III – head and tail lie in a straight line but the sperm head still has a partly expanded acrosome.(d) Mature – the head is aligned with and has a diameter similar to the tail. No motility was seen in the testicular sperm, but >60% of sperm from the cauda epididymes were motile. The stained preparations showed a significant difference in the distribution of sperm between the testis and epididymis (χ2 = 150.45, df = 3, P < 0.001), with sperm within the testis being skewed towards the earlier stages of development whereas the epididymis was skewed towards the later stages of development (Table 1). These results confirm the similarity with several other marsupials in that sperm are formed in the testis with the head at right angles to the tail but rotation of the head to become aligned with the tail is completed during epididymal transport. Work is now required to identify optimum conditions for the culture, cryopreservation and insemination of sperm from this species. (1)Cummins JM. (1976). Epididymal maturation of spermatozoa in the marsupial Trichsurus vulpecula: changes in motility and gross morphology. Aust. J. Zool. 24, 499–511.

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