Pesticide Residue Analysis in Fruit- and Vegetable-Based Baby Foods Using GC-Orbitrap MS

2018 ◽  
Vol 101 (2) ◽  
pp. 374-382 ◽  
Author(s):  
Ana Lozano ◽  
Samanta Uclés ◽  
Ana Uclés ◽  
Carmen Ferrer ◽  
Amadeo R Fernández-Alba
Keyword(s):  
Proficiency Test ◽  
Residue Analysis ◽  
Food Samples ◽  
Baby Food ◽  
The European Union ◽  
Baby Foods ◽  
Z Scores ◽  
Full Scan ◽  
Multiresidue Pesticide Analysis ◽  
Orbitrap Ms

Abstract This paper presents an efficiency evaluation of GC coupled with quadrupole Orbitrap MS for identification and quantitation in the multiresidue pesticide analysis of baby foods in full-scan mode. The identification criteria were studied following SANTE guidelines (retention time, mass accuracy, and ion ratio), comfortably complying with the values established, even at 0.003 mg/kg. Method validation was carried out on 15 selected GC-amenable pesticides covered by Commission Directive No. 2006/125/EC in three different baby food matrixes. Recovery studies were performed at 0.003 and 0.006 mg/kg, with 96% of the cases falling within the 70–120% range and with RSDs <15% for all the pesticides assayed. Linearity over 3 orders of magnitude was verified, with residuals <16% and correlation coefficient values >0.995. In general, matrix effect values were >100%. The LOQ was 0.003 mg/kg for 97% of the cases. The validated method was applied to 20 real baby food samples from Spain and to the European Union Proficiency Test FV-BF01 sample, in which the z-scores obtained were <1, thus demonstrating that this instrumentation has good quantitation capabilities.

scholarly journals Simultaneous Screening and Target Analytical Approach by Gas Chromatography-Quadrupole-Mass Spectrometry for Pesticide Residues in Fruits and Vegetables

2009 ◽  
Vol 92 (6) ◽  
pp. 1790-1806 ◽  
Author(s):  
Milagros Mezcua ◽  
Maria A Martínez-uroz ◽  
Philip L Wylie ◽  
Amadeo R fernández-alba
Keyword(s):  
Large Scale ◽  
Proficiency Test ◽  
Fruits And Vegetables ◽  
Screening Method ◽  
Correlation Coefficients ◽  
Food Samples ◽  
Identification Performance ◽  
Data Files ◽  
Full Scan ◽  
Scan Data

Abstract A full-scan GC/quadrupole/MS method has been developed to perform large-scale screenings of pesticides and simultaneous quantification of 95 target compounds in a single run of 21 min. The screening method was performed by using a deconvolution of the spectrum of the full-scan data files acquired under a retention time locked method. The identification performance of the screening method was evaluated in eight different food matrixes at three different concentrations. The system was equipped with a programmable temperature vaporizing inlet, allowing 10 L injections. The LOQ in the full-scan mode and linearity were studied for four different matrixes. Correlation coefficients >0.99 were achieved in all cases, and the LOD was <20 g/kg for 80 of the studied pesticides. Maintenance of the system was reduced by the use of a QuickSwap device that provided backflush capabilities by reversing column flow immediately after elution of the last compound of interest. The combined screening and target method was used in the analysis of more than 100 food samples, including a carrot sample from the European Proficiency Test FV 10, with good results.

Bacteriological Quality of Infant Milk Foods

1980 ◽  
Vol 43 (5) ◽  
pp. 340-342 ◽  
Author(s):  
R. S. SINGH ◽  
SUKHBIR SINGH ◽  
V. K. BATISH ◽  
B. RANGANATHAN
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Food Samples ◽  
Baby Food ◽  
Dnase Activity ◽  
Weaning Food ◽  
Baby Foods ◽  
Weaning Foods ◽  
Different Types

Ten samples of baby foods comprising seven brands of infant milk foods and three brands of milk-cereal weaning foods were examined for incidence of different types of microorganisms. One brand of infant milk food with 91 × 102 organisms also exhibited the maximum number of staphylococci and some of these were coagulase positive. Some staphylococcal isolates showed thermostable deoxyribonuclease (DNase) activity and also produced enterotoxins A or B. One sample of weaning food showed high counts of Bacillus cereus. In one of the reconstituted baby food samples when held at ambient temp. (37.5 C), the Staphylococcus aureus and B. cereus counts increased 10-fold in 3 h.

scholarly journals Occurrence and Exposure Assessment of Aflatoxins and Deoxynivalenol in Cereal-Based Baby Foods for Infants

Toxins ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 150 ◽  
Author(s):  
Marta Herrera ◽  
Noemi Bervis ◽  
Juan José Carramiñana ◽  
Teresa Juan ◽  
Antonio Herrera ◽  
...  
Keyword(s):  
Maximum Level ◽  
Food Samples ◽  
Baby Food ◽  
Free Products ◽  
Cereal Products ◽  
Baby Foods ◽  
Digestive Disorders ◽  
Daily Intakes ◽  
Infants And Young Children ◽  
The Eu

Aflatoxins are carcinogenic to humans and deoxynivalenol causes digestive disorders, and both mycotoxins occur frequently in cereal-based foods. The purpose of this study was to investigate the occurrence and levels of aflatoxins (B1, B2, G1 and G2) and deoxynivalenol (DON) in cereal-based baby foods as well as to calculate the estimated daily intakes (EDI) in different stages of infancy. Sixty samples of infant cereals (wheat-, corn-, rice-, oat-, and mixed grain-based) were collected during a 2-year period and analyzed by validated methods. Aflatoxins were detected in 12 samples (20%), six of which exceeded the EU maximum level for aflatoxin B1 set at 0.10 µg/kg. Deoxynivalenol appeared in 20% of baby food samples, with one sample exceeding the EU maximum level established at 200 µg/kg. There were no significant differences between gluten-free products for babies aged 4–6 months and multi-cereal products for infants aged 7–12 months, nor between whole-grain-based and refined ingredients. However, baby food products of organic origin showed significantly higher levels of deoxynivalenol than conventional ones (p < 0.05). It is proposed for the health protection of infants and young children, a vulnerable group, to establish the lowest maximum level for the sum of aflatoxins (B1, B2, G1 and G2) in baby food.

Survey of Total Tetrahydrophthalimide in Baby Foods Using Both Enzyme-Linked Immunosorbent Assay and Gas Chromatography/Mass Spectrometry: A Comparative Study

1993 ◽  
Vol 76 (6) ◽  
pp. 1225-1229 ◽  
Author(s):  
Jupiter M Yeung ◽  
W Harvey Newsome
Keyword(s):  
Immunosorbent Assay ◽  
Food Samples ◽  
Baby Food ◽  
Gas Chromatography Mass Spectrometry ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Assay ◽  
Elisa Method ◽  
Baby Foods ◽  
Fruit Samples ◽  
Chromatographic Mass

Abstract An enzyme-linked immunosorbent assay (ELISA) method was compared with a gas chromatographic/ mass spectrometric (GC/MS) method for determining the concentration (in parts per million) of the combination of captan and its degradation product tetrahydrophthalimide (THPI) in 13 fruit samples and in a survey of baby foods. Ninety baby foods (49 fruits, 28 juices, and 13 vegetables) from 2 different suppliers were sampled. All captan in the samples was converted to THPI before each analysis. None of the samples contained a concentration of combined captan and THPI that violated the maximum residue limit of 5.0 ppm. Eight samples of baby food tested positive for THPI at levels ranging from 0.019-0.041 ppm by the GC/MS method, whereas 20 samples tested positive in the ELISA assay. All samples that tested positive with the GC/MS method also tested positive with the ELISA method. Thirteen percent of the baby food samples tested false positive with the ELISA method. The ELISA assay also gave higher values than the GC/MS method. The ELISA method can be effectively used as a primary screening tool to select samples testing positive for THPI. The concentration of THPI in these samples can then be verified using the GC/MS method.

scholarly journals Systematic Profiling of the Multicomponents and Authentication of Erzhi Pill by UHPLC/Q-Orbitrap-MS Oriented Rapid Polarity-Switching Data-Dependent Acquisition and Selective Monitoring of the Chemical Markers Deduced from Fingerprint Analysis

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3143 ◽  
Author(s):  
Li Jia ◽  
Lingling Fu ◽  
Xiaoyan Wang ◽  
Wenzhi Yang ◽  
Hongda Wang ◽  
...  
Keyword(s):  
High Efficiency ◽  
High Energy ◽  
Composition Analysis ◽  
Chemical Markers ◽  
Polarity Switching ◽  
Selective Ion Monitoring ◽  
Marker Compounds ◽  
Full Scan ◽  
Selective Monitoring ◽  
Orbitrap Ms

The analytical platform UHPLC/Q-Orbitrap-MS offers a solution to quality investigation of TCM with high definiteness. Using Erzhi Pill (EZP) as a case, we developed UHPLC/Q-Orbitrap-MS based approaches to achieve systematic multicomponent identification and rapid authentication. Comprehensive multicomponent characterization of EZP was performed by negative/positive switching data-dependent high-energy collision-induced dissociation-MS2 (HCD-MS2) after 25 min chromatographic separation. By reference compounds comparison, elemental composition analysis, fragmentation pathways interpretation, and retrieval of an in-house library, 366 compounds were separated and detected from EZP, and 96 thereof were structurally characterized. The fingerprints of two component drugs (Ligustri Lucidi Fructus, LLF; Ecliptae Herba, EH) for EZP were analyzed under the same LC-MS condition by full scan in negative mode. In combination with currently available pharmacological reports, eight compounds were deduced as the ‘identity markers’ of EZP. Selective ion monitoring (SIM) of eight marker compounds was conducted to authenticate six batches of EZP samples. Both LLF and EH could be detected from all EZP samples by analyzing the SIM spectra, which could indicate their authenticity. Conclusively, UHPLC/Q-Orbitrap-MS by rapid polarity switching could greatly expand the potency of untargeted profiling with high efficiency, and SIM of multiple chemical markers rendered a practical approach enabling the authentication of TCM formulae.

Comparative analysis of chemical contamination of baby foods and primary pediatric morbidity

2021 ◽  
Author(s):  
YuL Tikhonova
Keyword(s):  
Pearson Correlation ◽  
Food Contamination ◽  
Baby Food ◽  
Endocrine Disorders ◽  
Chemical Contamination ◽  
Microsoft Excel ◽  
Baby Foods ◽  
Microsoft Word ◽  
Using Data ◽  
Pediatric Morbidity

The safety of baby foods is key to a child’s health, which, in turn, is one of the prioritized national goals. As they grow, children get exposed to numerous negative environmental impacts. Chemical contamination of baby foods can increase pediatric morbidity. The aim of this study was to investigate possible correlations between baby food contamination and primary pediatric morbidity using data on 65 Russian regions collected in 2012–2017 by the Russian Federal Information Public Health Surveillance Foundation. The data were processed in Microsoft Word 2010 and Microsoft Excel 2010. Of 67,940 samples of baby foods analyzed for chemical contamination, priority pollutants (toxic element) were detected in 14.1%. The most contaminated were fruit and vegetable purees (47.1%), followed by milk formulas and cultured dairy products (19.9%). We also analyzed 32,914 indicators of pediatric morbidity. The Pearson correlation analysis detected reliable correlations between baby food contamination and the primary incidence of endocrine disorders in infants, as well as the primary incidence of obesity, diabetes mellitus and cancer in children aged 0 to 14 years.

scholarly journals Model Proficiency Testing Scheme for Serological Diagnosis of Brucellosis: Interlaboratory Study

2004 ◽  
Vol 87 (4) ◽  
pp. 965-971 ◽  
Author(s):  
Donatella Nannini ◽  
Manuela Tittarelli ◽  
Lucilla Ricci ◽  
Annamaria Conte ◽  
Bernardo Di Emidio ◽  
...  
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reference Laboratory ◽  
Correct Result ◽  
The European Union ◽  
Serological Tests ◽  
Testing Scheme ◽  
Z Scores ◽  
Qualitative Test

Abstract A model interlaboratory testing scheme was developed by the Italian National Reference Laboratory for Brucellosis. This scheme was planned for both qualitative (Rose Bengal Plate Test; RBPT) and quantitative (Complement Fixation Test; CFT) serological tests and involved a total of 42 laboratories. In the preparation of this scheme, reference was made to general protocols and guidelines and to methods reported in the literature, which were applicable to analytical chemistry laboratories. Six field sera from naturally infected animals, one positive serum at a titer below the European Union (EU) positivity threshold, and 5 sera positive at titers between 20 and 851 International Units of Complement Fixation Test (IUCFT)/mL plus one negative serum were used to produce a panel of test sera. To evaluate laboratory performances in the quantitative test for each tested sample examined, z-scores based on robust summary statistics (the median and normalized interquartile range) were used. To evaluate overall laboratory performance, 2 types of combined z-scores were used: Rescaled Sum of Scores and Sum of Squared Scores. In the case of the qualitative test (RBPT), results were analyzed by a Bayesian approach. A Beta distribution, based on the result of each laboratory, was calculated and used to estimate the probability of each laboratory giving a correct result and its uncertainty.

Laboratory performance and regulatory requirements.

1987 ◽  
Vol 33 (6) ◽  
pp. 1087-1102 ◽  
Author(s):  
M L Kenney
Keyword(s):  
Linear Regression ◽  
Proficiency Test ◽  
Clinical Laboratory ◽  
Group Practice ◽  
Federal Regulations ◽  
Test Results ◽  
Regulatory Requirements ◽  
Service Levels ◽  
Z Scores ◽  
Log Normal

Abstract We compared performance levels of four clinical laboratory groups defined by federal regulatory characteristics, to assess the appropriateness of selected regulations: laboratories in JCAH-accredited hospitals; non-doctoral-directed independent laboratories; state-regulated but federally exempt group-practice laboratories; and unregulated laboratories in physicians' offices (POLs). Federal regulations evaluated were those dealing with the doctoral directorship requirement and exemption of POLs from regulation. Quantitative analytes were compared by using linear regression on log-normal transformations of mean absolute-z scores of proficiency test results. The scope of services offered by laboratories was statistically related to performance in quantitative analytes. Confounding effects of scope-of-service levels were statistically controlled. Proportions of errors in qualitative analytes were compared. No pattern of statistically discernible differences in performance was found between hospital laboratories and non-doctoral-directed laboratories. Both regulated non-doctoral-directed laboratories and state-regulated but federally exempt group-practice laboratories demonstrated higher levels of performance than unregulated POLs.

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