scholarly journals Comparative Fingerprint Profiling of Unani Polyherbomineral (Safoof-e-Pathar Phori) Formulation by HPTLC, HPLC, and GC-MS

2020 ◽  
Vol 103 (3) ◽  
pp. 659-668 ◽  
Author(s):  
Wasim Ahmad ◽  
Rabea Parveen ◽  
Mohd Mujeeb ◽  
S M Arif Zaidi
Keyword(s):  
High Performance ◽  
Potassium Nitrate ◽  
Retardation Factor ◽  
Quality And Safety ◽  
Fingerprint Analysis ◽  
Dolichos Biflorus ◽  
Relative Retention ◽  
Plant Constituents ◽  
Mineral Constituents

Abstract Background: Standardization of Unani formulations is essential to assess the quality and safety of drugs to establish their acceptance internationally as well as in nationally. Safoof-e-Pathar phori is a polyherbomineral formulation that contains six different plant and mineral constituents: Didymocarpus pedicellata, Dolichos biflorus, Rheum emodi, Raphanus sativus, potassium carbonate, and potassium nitrate. Objective: The objective of the study is to develop and establish the quality of Safoof-e-Pathar phori, used for antilithiatic activity using chromatographic analysis. Methods: This study involves comparative fingerprint profiling of an Unani polyherbomineral formulation with its plant constituents by high-performance TLC (HPTLC), HPLC, and GC-MS. The authenticity of the Unani polyherbomineral formulation using comparative HPLC and GC-MS fingerprint profiling was achieved by comparing the retention time of peaks matching with plant drugs. Results: In HPTLC, comparative fingerprinting was done on the basis of retardation factor values of compounds separated on HPTLC plates of the formulation and the single drugs. The relative retention times of the characteristic peaks in the HPLC fingerprinting were established for identification of the Unani poly-herbo-mineral formulation, whereas GC-MS was used for comparative fingerprint profiling of different compounds present in single drugs and in formulation through the National Institute of Standards and Technology and Wiley libraries using mass by charge ration (m/z) of detected compounds. Conclusions: The fingerprint analysis approach is the most potent tool for quality control of polyherbal medicines because of its accuracy and reliability, and it also helps in identification and authentication and prevents adulteration. Highlights: The developed chromatographic methods enable the quality and safety profile of the products.

scholarly journals A Strategy for Quality Control of Vespa magnifica (Smith) Venom Based on HPLC Fingerprint Analysis and Multi-Component Separation Combined with Quantitative Analysis

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2920 ◽  
Author(s):  
Si-Tong Zhou ◽  
Kai Luan ◽  
Lian-Li Ni ◽  
Ying Wang ◽  
Shi-Meng Yuan ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
High Performance ◽  
Principal Component ◽  
Good Precision ◽  
Fingerprint Analysis ◽  
Wasp Venom ◽  
Chemical Fingerprint ◽  
Hplc Fingerprint ◽  
Relative Standard

As a folk medicine of the Jingpo minority in Yunnan province, the venom of Vespa magnifica has been commonly used for the treatment of rheumatoid arthritis. Quality standardization of the wasp venom is a necessary step for its pharmaceutical research and development. To control the quality of the wasp venom, a method based on high-performance liquid chromatography (HPLC) was developed for chemical fingerprint analysis. In the chromatographic fingerprinting, chemometrics procedures, including similarity analysis (SA), hierarchical clustering analysis (HCA), and principal component analysis (PCA), were applied to classify 134 batches (S1–S134) of wasp venom from different origins. The HPLC fingerprint method displayed good precision (Relative standard deviation, RSD < 0.27%), stability (in 16 h, RSD < 0.34%), and repeatability (RSD < 1.00%). Simultaneously, four compounds (VMS1, VMS2, VMS3, and VMS4) in the wasp venom were purified and identified. VMS1 was 5-hydroxytryptamine, and the other compounds were three peptides that were sequenced as follows: Gly–Arg–Pro–Hyp–Gly–Phe–Ser–Pro–Phe–Arg–Ile–Asp–NH2 (VMS2), Ile–Asn–Leu–Lys–Ala–Ile–Ala–Ala–Leu–Ala–Lys–Lys–Leu–Leu–NH2 (VMS3), and Phe–Leu–Pro–Ile–Ile–Gly–Lys–Leu–Leu–Ser–Gly–Leu–Leu–NH2 (VMS4). The quantifications for these components were 110.2 mg/g, 26.9 mg/g, 216.3 mg/g, and 58.0 mg/g, respectively. The results of this work indicated that the combination of the chemical fingerprint and quantitative analysis offers a reasonable way to evaluate the quality of wasp venom.

Free Amino Acids Fingerprint Analysis for the Quality Evaluation of Oriental Latrine Fly Larvina by RP-HPLC Pre-Column Derivatization Method

2013 ◽  
Vol 781-784 ◽  
pp. 1842-1846
Author(s):  
Yu Ping Wu ◽  
Fu Jiang Chu ◽  
Xiao Bao Jin ◽  
Wen Bin Liu ◽  
Yin Ye Xu ◽  
...  
Keyword(s):  
Free Amino ◽  
High Performance ◽  
Quality Evaluation ◽  
Control Method ◽  
Gradient Elution ◽  
Fingerprint Analysis ◽  
Chromatographic Fingerprint ◽  
Rp Hplc ◽  
Quality Control Method

A simple and reliable method of high-performance liquid chromatography with pre-column derivatization was developed to evaluate the quality of Oriental Latrine Fly Larvina through establishing chromatographic fingerprint. The separation was performed on a welch Ultimate XB-C8 column with a liner gradient elution of acetonitrile and acetate buffer. The developed method was validated by precision, stability and recovery. This study indicated that the chromatographic fingerprint analysis can be readily utilized as a quality control method for oriental latrine fly larvina.

scholarly journals Chemical Fingerprint Analysis and Quantitative Analysis of Saccharides in Morindae Officinalis Radix by HPLC-ELSD

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7242
Author(s):  
Hongmei Sun ◽  
Yini Cai ◽  
Jie Shen ◽  
Enyao Ma ◽  
Zhimin Zhao ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Ion Mobility ◽  
High Performance ◽  
Gradient Elution ◽  
Fingerprint Analysis ◽  
Chemical Fingerprint ◽  
Hplc Fingerprint ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering

A method based on high performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for the quantitative analysis of three active compounds and chemical fingerprint analyses of saccharides in Morindae officinalis radix. Ten batches of Morindae officinalis radix were collected from different plantations in the Guangdong region of China and used to establish the fingerprint. The samples were separated with a COSMOIL Sugar-D column (4.6 mm × 250 mm, 5 μm) by using gradient elution with water (A) and acetonitrile (B). In addition, Trapped-Ion-Mobility (tims) Time-Of-Flight (tims TOF) was used to identify saccharides of Morindae officinalis radix. Fingerprint chromatogram presented 26 common characteristic peaks in the roots of Morinda officinalis How, and the similarities were more than 0.926. In quantitative analysis, the three compounds showed good regression (r = 0.9995–0.9998) within the test ranges, and the recoveries of the method were in the range of 96.7–101.7%. The contents of sucrose, kestose and nystose in all samples were determined as 1.21–7.92%, 1.02–3.37%, and 2.38–6.55%, respectively. The developed HPLC fingerprint method is reliable and was validated for the quality control and identification of Morindae officinalis radix and can be successfully used to assess the quality of Morindae officinalis radix.

THE EFFECT OF PROCESSING METHODS ON THE NUTRITIONAL QUALITY OF AFRICAN BREADFRUITS (TRECULIA AFRICANA) SEEDS

2020 ◽  
Vol 16 (2) ◽  
pp. 60
Author(s):  
Nwozo Sarah Onyenibe ◽  
Julius Oluwaseun Oluwafunmilola ◽  
Stanley Udogadi Nwawuba
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Nutritional Quality ◽  
High Performance ◽  
Phytochemical Analysis ◽  
Mineral Contents ◽  
Processing Methods ◽  
African Breadfruit

The extracted seeds of African breadfruit are identified to be extremely healthy whenever it is correctly processed. Therefore, the aim of the present study was to evaluate the effects of processing methods on the nutritional quality of African breadfruit seed. A qualitative phytochemical analysis including: Alkaloid, Flavonoid, Saponin, Tannin, Anthraquinone, Terpenoids, Steroid, and Cardiac Glycosides for the different fraction of African breadfruit seed was performed using a standard method. The result revealed the presence and greater amount of phytochemical for the raw fraction; seven in eight, six in eight for steamed fraction, and four in eight for boiled and roasted respectively. Anti-nutrient, Proximate, and Mineral Content were also conducted using standard methods. The amino acid composition was determined using High-Performance Liquid Chromatography (HPLC). The results of the present study revealed that anti-nutrients including Phytate, Tannins, and Oxalate were significantly p<0.05 reduced in the boiled fraction 5.47±0.15, 3.42±0.02 and 6.89±0.05, and highest in the raw fraction 7.77±0.01, 5.09±0.03 and 9.34±0.14. The proximate composition including; percentage crude fat, Ash, Carbohydrate, Fatty acid, and Energy value were significantly lower p<0.05 in the boiled fraction relative to the other fractions. Mineral contents; calcium, magnesium, sodium, potassium, and phosphorus were also significantly p<0.05 elevated in the boiled fraction relative to the raw, steamed, and roasted fraction. The amino acid composition was highest in the roasted and boiled fraction 57.350 and 56.978, and lowest in the steamed and raw fraction 35.754 and 28.748 respectively. Therefore, boiling (cooking) is encouraged for the preparation of African breadfruit seed.

scholarly journals Development and application of a spectrophotometric method in quality evaluation of benzimidazole anthelminthics in Nairobi city county

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Johnson K. Murage ◽  
Beatrice K. Amugune ◽  
Peter Njogu ◽  
Stanley Ndwigah
Keyword(s):  
Spectrophotometric Method ◽  
High Performance ◽  
Neglected Tropical Diseases ◽  
Developing Economies ◽  
Raw Materials ◽  
Poor Quality ◽  
Prolonged Treatment ◽  
Good Precision ◽  
Market Surveillance

Abstract Background Neglected tropical diseases (NTDs) are a group of communicable diseases which are prevalent in the tropics affecting more than one billion people. Treatment and prevention of these infections is very costly to developing economies. Helminthiases are classified among NTDs. The communities afflicted are poor and have limited access to essential resources for their livelihood. Poor-quality drugs for NTDs may lead to death or prolonged treatment without achieving the desired results. The limited resources used in purchasing poor-quality drugs will therefore be wasted instead of being put to good use. Most of the methods available for the analysis of benzimidazole anthelminthics utilize high-performance liquid chromatography. They are therefore time consuming, require sophisticated and expensive equipment, utilize rare and expensive reagents and solvents, and call for skilled personnel. A simple, rapid, and inexpensive ultraviolet spectrophotometric method of analysis would therefore come in handy especially in the analysis of many samples as occurs during post-authorization market surveillance for quality. Results The suitable solvent for the spectroscopic analysis was established as 0.1 M methanolic HCl. The wavelength of analysis was set at 294 nm. Upon validation, the method was found to have good linearity. The range over which linearity was established was way beyond the 80 to 120% of the working concentration specified by the ICH. The method exhibited good precision. Out of 32 commercial samples analyzed, five (15.6%) did not comply with compendial specifications. Intra-brand batch variation was also observed. Out of three batches of product A002T analyzed, one did not comply with compendial specifications. Conclusion A major limitation in the analysis of benzimidazole anthelminthics is the lack of reliable, simple, rapid, and low-cost methods of analysis with high throughput. The developed method serves to fill this gap. It can be used in the analysis of raw materials and finished products. It can also be used in the establishment of the quality of products prior to registration. The method will prove very useful in post-market surveillance of quality of benzimidazole anthelminthics.

A Study on the Mineral Depletion of the Foods Available to us as a Nation over the Period 1940 to 1991

2003 ◽  
Vol 17 (2) ◽  
pp. 85-115 ◽  
Author(s):  
David Thomas
Keyword(s):  
Mineral Content ◽  
Dietary Habits ◽  
Medical Research Council ◽  
Significant Loss ◽  
King's College ◽  
Compare And Contrast ◽  
Insight Into ◽  
Mineral Constituents ◽  
Mineral Depletion

In 1927 a study at King's College, University of London, of the chemical composition of foods was initiated by Dr McCance to assist with diabetic dietary guidance. The study evolved and was then broadened to determine all the important organic and mineral constituents of foods, it was financed by the Medical Research Council and eventually published in 1940. Over the next 51 years subsequent editions reflected changing national dietary habits and food laws as well as advances in analytical procedures. The most recent (5th Edition) published in 1991 has comprehensively analysed 14 different categories of foods and beverages. In order to provide some insight into any variation in the quality of the foods available to us as a nation between 1940 and 1991 it was possible to compare and contrast the mineral content of 27 varieties of vegetable, 17 varieties of fruit, 10 cuts of meat and some milk and cheese products. The results demonstrate that there has been a significant loss of minerals and trace elements in these foods over that period of time. It is suggested that the results of this study cannot be taken in isolation from recent dietary, environmental and disease trends. These trends are briefly mentioned and suggestions are made as to how the deterioration in the micronutrient quality of our food intake may be arrested and reversed.

Robust Environmental Sensing Using UAVs

2021 ◽  
Vol 2 (4) ◽  
pp. 1-20
Author(s):  
Ahmed Boubrima ◽  
Edward W. Knightly
Keyword(s):  
Air Pollution ◽  
Gas Sensors ◽  
High Performance ◽  
Weather Conditions ◽  
Ambient Air ◽  
Mission Planning ◽  
Pollution Monitoring ◽  
Compound Pollution ◽  
Planning Algorithm

In this article, we first investigate the quality of aerial air pollution measurements and characterize the main error sources of drone-mounted gas sensors. To that end, we build ASTRO+, an aerial-ground pollution monitoring platform, and use it to collect a comprehensive dataset of both aerial and reference air pollution measurements. We show that the dynamic airflow caused by drones affects temperature and humidity levels of the ambient air, which then affect the measurement quality of gas sensors. Then, in the second part of this article, we leverage the effects of weather conditions on pollution measurements’ quality in order to design an unmanned aerial vehicle mission planning algorithm that adapts the trajectory of the drones while taking into account the quality of aerial measurements. We evaluate our mission planning approach based on a Volatile Organic Compound pollution dataset and show a high-performance improvement that is maintained even when pollution dynamics are high.

Pursuit of Operational Excellence

2016 ◽  
Vol 3 (2) ◽  
pp. 21-34
Author(s):  
Christine Welch ◽  
Tammi Sinha ◽  
Nigel Ward
Keyword(s):  
Change Management ◽  
Continuous Improvement ◽  
High Performance ◽  
Effective Leadership ◽  
Value Systems ◽  
Goods And Services ◽  
Team Working ◽  
Performance Teams ◽  
Operational Excellence

Operational Excellence (OE) is achieved when high performance teams are seeking for continuous improvement in well-designed processes, using appropriate tools and technologies. Excellence is underpinned by a philosophy in which problem-solving, team-working and effective leadership combine to focus upon customer needs, and all employees are empowered to act to maintain optimal flows of value. OE is clearly a desirable quality of organizations seeking both effectiveness and efficiency in their production of goods and services for customers. OE is underpinned by concepts such as team-working, effective leadership and change management, and depends upon effective flows of value. Systems Thinking (ST) is consequently at the heart of genuine excellence. This paper was conceived in the context of a Community of Practice of business improvement professionals, who took Operational Excellence as their agenda for inquiry during sessions in 2015. Reflection upon practice discussed at these meetings, together with the literature of change management and continuous improvement, have led to development of a systemic ‘landscape' model for pursuit of Operational Excellence. The elements of this model are set out, showing how they can contribute to OE.

scholarly journals A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee

2007 ◽  
Vol 50 (2) ◽  
pp. 349-359 ◽  
Author(s):  
Simone Fujii ◽  
Elisabete Yurie Sataque Ono ◽  
Ricardo Marcelo Reche Ribeiro ◽  
Fernanda Garcia Algarte Assunção ◽  
Cássia Reika Takabayashi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Ochratoxin A ◽  
High Performance ◽  
Screening Tool ◽  
Correlation Coefficients ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Assay ◽  
Quality And Safety ◽  
Green Coffee ◽  
Instant Coffee

An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) and HRP-anti IgG (10³-fold dilution). The detection limit was 3.73 ng OTA/g and correlation coefficients (r) between this immunoassay and high performance liquid chromatography were 0.98 for green coffee, 0.98 for roasted and 0.86 for instant. OTA levels detected by ic-ELISA were higher than by HPLC, with ELISA/HPLC ratio of 0.66 - 1.46 (green coffee), 0.96 - 1.11 (roasted) and 0.93 - 1.82 (instant). ELISA recoveries for OTA added to coffee (5 - 70 ng/g) were 81.53 % for green coffee, 46.73 % for roasted and 64.35 % for instant, while recoveries by HPLC were 80.54 %, 45.91 % and 55.15 %, respectively. Matrices interferences were minimized by samples dilution before carrying out the ELISA assay. The results indicate that MAb-based ic-ELISA could be a simple, sensitive and specific screening tool for OTA detection, contributing to quality and safety of coffee products.

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