Application of Green Extraction Techniques for Natural Additives Production

During the last decades, consumers have increased the demand for healthier natural foods with lower presence of chemical additives. One reason of this choice is the controversy about chemical additives possible adverse effects. To fulfill market needs, different techniques have been developed to extract compounds from various raw materials to produce natural additives with different properties (preservatives, emulsifiers, or colorants) and bioactivities. In addition, the growing concern about the effects of climate change has led the development of more sustainable techniques to carry out the extraction. The use of new alternative nonconventional, emerging, or green extraction methodologies has gained considerable attention during the last decade. These novel techniques have been applied to minimize any negative changes in the nutritional, physicochemical or sensory properties of the natural source, while at the same time reducing the environmental impact of the process and gaining competitiveness of the world market. For this purpose, new green extraction methods have been proposed and optimized for the reduction of the consumption of raw materials, solvents, and energy. In this chapter, a revision of different types of green extraction techniques is compiled together with the main factor that can affect extraction-process feasibility and the main challenges and future trends for their development.

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Integrated Metabolomics and Transcriptomics Using an Optimised Dual Extraction Process to Study Human Brain Cancer Cells and Tissues

Metabolites ◽

10.3390/metabo11040240 ◽

2021 ◽

Vol 11 (4) ◽

pp. 240

Author(s):

Alison Woodward ◽

Alina Pandele ◽

Salah Abdelrazig ◽

Catherine A. Ortori ◽

Iqbal Khan ◽

...

Keyword(s):

Metabolic Pathways ◽

Extraction Method ◽

Limit Of Detection ◽

Rna Extraction ◽

Extraction Process ◽

Extraction Methods ◽

Serum Starvation ◽

Extraction Techniques ◽

Metabolic Genes ◽

Dual Extraction

The integration of untargeted metabolomics and transcriptomics from the same population of cells or tissue enhances the confidence in the identified metabolic pathways and understanding of the enzyme–metabolite relationship. Here, we optimised a simultaneous extraction method of metabolites/lipids and RNA from ependymoma cells (BXD-1425). Relative to established RNA (mirVana kit) or metabolite (sequential solvent addition and shaking) single extraction methods, four dual-extraction techniques were evaluated and compared (methanol:water:chloroform ratios): cryomill/mirVana (1:1:2); cryomill-wash/Econospin (5:1:2); rotation/phenol-chloroform (9:10:1); Sequential/mirVana (1:1:3). All methods extracted the same metabolites, yet rotation/phenol-chloroform did not extract lipids. Cryomill/mirVana and sequential/mirVana recovered the highest amounts of RNA, at 70 and 68% of that recovered with mirVana kit alone. sequential/mirVana, involving RNA extraction from the interphase of our established sequential solvent addition and shaking metabolomics-lipidomics extraction method, was the most efficient approach overall. Sequential/mirVana was applied to study a) the biological effect caused by acute serum starvation in BXD-1425 cells and b) primary ependymoma tumour tissue. We found (a) 64 differentially abundant metabolites and 28 differentially expressed metabolic genes, discovering four gene-metabolite interactions, and (b) all metabolites and 62% lipids were above the limit of detection, and RNA yield was sufficient for transcriptomics, in just 10 mg of tissue.

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Evaluation of the Efficacy of Various Green Extraction Methods for High Valorisation of Vegetal Antioxidant Sources

Revista de Chimie ◽

10.37358/rc.18.10.6607 ◽

2018 ◽

Vol 69 (10) ◽

pp. 2708-2711

Author(s):

Georgiana I. Badea ◽

Simona C. Litescu Filipescu ◽

Gabriel L. Radu ◽

Ioana Diaconu

Keyword(s):

Raw Materials ◽

Extraction Methods ◽

Hplc Method ◽

Complete Characterization ◽

Antioxidant Compounds ◽

Implementation Cost ◽

Green Extraction ◽

Efficacy Assessment ◽

By Products

This work presents the efficacy of various green extraction methods used to ensure the optimal amount of antioxidant compounds extracted from vegetal material. The new developed extraction procedures are easy-to-use and have a low implementation cost providing health-safe and high-quality extracts (products). The complete characterization of the obtained extracts was performed by a HPLC method (quantification of antioxidant compounds) and the ORAC method (efficacy assessment). These methodologies have high potential in by-products industry, where managing the residues is an important step in increasing the technological process economic efficiency and mitigation of environmental impact and, moreover, can by further extended to other types of raw materials.

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Screening of Extraction Process and The Estimation of Total Alkaloids in Carica papaya Linn. Leaf

Majalah Obat Tradisional ◽

10.22146/mot.52184 ◽

2020 ◽

Vol 25 (2) ◽

pp. 90

Author(s):

Sisca Devi ◽

Meri Ropiqa ◽

Yosi Bayu Murti ◽

Akhmad Kharis Nugroho

Keyword(s):

Carica Papaya ◽

Raw Materials ◽

Ammonium Hydroxide ◽

Extraction Process ◽

Extraction Methods ◽

Traditional Treatment ◽

Total Alkaloids ◽

Water Solvent ◽

Layer Chromatography

The papaya leaf decoctions have been widely used as a traditional treatment for malaria. The alkaloid of papaya leaves, carpaine, is reported to have the antiplasmodial activity in vitro. The industrial-scale commercial product development requires the quantification of the carpaine, and total alkaloids in the papaya leave as the raw materials. This study aims to optimize the extraction process leading to the highest yield and total alkaloids. The extraction process was carried out using different methods and solvents. The first method was maceration with the water-miscible solvent of ethanol: distilled water: HCl 37% (89: 10: 1 v/v/v). The second method was maceration with the water-immiscible solvent of dichloromethane solvents in pH 8-9 with ammonium hydroxide. The third method was digestion with acid water solvent of 0.05 N HCl. The identification of alkaloids employed the analysis using Thin Layer Chromatography (TLC) and Dragendroff reagent. The quantification of carpaine used a densitometer, while the spectrophotometric method was used to estimate the total alkaloids. The results showed that despite the extraction methods used, the tested extracts confirm the alkaloids content with the highest yield of 3.09% (84.72% of total alkaloids).

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In Vitro Bioactivity of Astaxanthin and Peptides from Hydrolisates of Shrimp (Parapenaeus longirostris) By-Products: From the Extraction Process to Biological Effect Evaluation, as Pilot Actions for the Strategy “From Waste to Profit”

Marine Drugs ◽

10.3390/md19040216 ◽

2021 ◽

Vol 19 (4) ◽

pp. 216

Author(s):

Concetta Maria Messina ◽

Simona Manuguerra ◽

Rosaria Arena ◽

Giuseppe Renda ◽

Giovanna Ficano ◽

...

Keyword(s):

Bioactive Compounds ◽

Bioactive Peptides ◽

Extraction Process ◽

Extraction Methods ◽

Ethyl Esters ◽

Model Systems ◽

Protective Effects ◽

Green Extraction ◽

By Products

Non-edible parts of crustaceans could be a rich source of valuable bioactive compounds such as the carotenoid astaxanthin and peptides, which have well-recognized beneficial effects. These compounds are widely used in nutraceuticals and pharmaceuticals, and their market is rapidly growing, suggesting the need to find alternative sources. The aim of this work was to set up a pilot-scale protocol for the reutilization of by-products of processed shrimp, in order to address the utilization of this valuable biomass for nutraceutical and pharmaceuticals application, through the extraction of astaxanthin-enriched oil and antioxidant-rich protein hydrolysates. Astaxanthin (AST) was obtained using “green extraction methods,” such as using fish oil and different fatty acid ethyl esters as solvents and through supercritical fluid extraction (SFE), whereas bioactive peptides were obtained by protease hydrolysis. Both astaxanthin and bioactive peptides exhibited bioactive properties in vitro in cellular model systems, such as antioxidant and angiotensin I converting enzyme (ACE) inhibitory activities (IA). The results show higher astaxanthin yields in ethyl esters fatty acids (TFA) extraction and significant enrichment by short-path distillation (SPD) up to 114.80 ± 1.23 µg/mL. Peptide fractions of <3 kDa and 3–5 kDa exhibited greater antioxidant activity while the fraction 5–10 kDa exhibited a better ACE-IA. Lower-molecular-weight bioactive peptides and astaxanthin extracted using supercritical fluids showed protective effects against oxidative damage in 142BR and in 3T3 cell lines. These results suggest that “green” extraction methods allow us to obtain high-quality bioactive compounds from large volumes of shrimp waste for nutraceutical and pharmaceutical applications.

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Applications of Nonconventional Green Extraction Technologies in Process Industries: Challenges, Limitations and Perspectives

Sustainability ◽

10.3390/su12135244 ◽

2020 ◽

Vol 12 (13) ◽

pp. 5244

Author(s):

Gertrude Fomo ◽

Tafirenyika Nyamayaro Madzimbamuto ◽

Tunde Victor Ojumu

Keyword(s):

Electric Fields ◽

Supercritical Co2 ◽

Raw Materials ◽

Value Added ◽

Extraction Process ◽

Extraction Methods ◽

Extraction Yield ◽

Process Industries ◽

Functional Value ◽

Work Done

This study reviewed five different nonconventional technologies which are aligned with green concepts of product recovery from raw materials on industrial scale, with minimal energy consumption and chemical use. Namely, this study reviewed supercritical fluid extraction (SCFE), pressurized liquid extraction (PLE), microwave-assisted extraction (MAE), ultrasound extraction (UAE) and pulsed electric fields extraction (PEFE). This paper provides an overview of relevant innovative work done in process industries on different plant matrices for functional value-added compounds and byproduct production. A comparison of the five extraction methods showed the supercritical CO2 (SC-CO2) process to be more reliable despite some limitations and challenges in terms of extraction yield and solubility of some bioactive compounds when applied in processing industries. However, these challenges can be solved by using ionic liquids as a trainer or cosolvent to supercritical CO2 during the extraction process. The choice of ionic liquid over organic solvents used to enhance extraction yield and solubility is based on properties such as hydrophobicity, polarity and selectivity in addition to a safe environment.

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ADAPTATION AND DEVELOPMENT OF MILK PRODUCERS IN THE NEW ECONOMIC CONDITIONS

Molochnoe i miasnoe skotovodstvo ◽

10.33943/mms.2020.37.77.001 ◽

2020 ◽

Author(s):

В.Н. СУРОВЦЕВ ◽

Е.Н. ПАЮРОВА

Keyword(s):

Dairy Products ◽

Raw Materials ◽

Raw Milk ◽

World Market ◽

Food Prices ◽

Economic Conditions ◽

State Support ◽

Demand Reduction ◽

The World ◽

Dairy Cattle Breeding

Проанализированы проблемы на рынке молока и молочных продуктов России в условиях глобального экономического кризиса: перепроизводство молока в основных странах-экспортерах, снижение закупочных цен на сырое молоко, снижение цен на биржевые товары в мире и России в 2020 году, тренд на уменьшение спроса на молочные продукты на мировом рынке в среднесрочном периоде, снижение общего спроса на молочные продукты на внутрироссийском рынке при падении реальных доходов населения, изменение структуры потребления. Проведена оценка новых возможностей и угроз для развития отрасли: со стороны потребителей — рост цен на продовольствие, снижение доходов; с позиции производителей молока — снижение закупочных цен, рост требований к сырью для производства продукции с увеличенными сроками годности, дефицит рабочей силы, вероятное сокращение господдержки в результате снижения цен на углеводороды, попытки регулирования цен; со стороны перерабатывающих предприятий — сокращение спроса, снижение цен на готовую продукцию вслед за мировыми ценами. Обоснованы приоритеты инвестирования в молочном скотоводстве и основные формы совершенствования государственной поддержки отрасли, обеспечивающие эффективную адаптацию производителей молока к новым экономическим условиям, повышение устойчивости отрасли при усилении макроэкономических рисков. The article analyzes the problems in the Russian milk and dairy products market in the context of the global economic crisis: overproduction of milk in the main exporting countries, lower purchase prices for raw milk, lower prices for commodities in the world and in Russia in 2020, trend to reduce demand for dairy products on the world market in medium term, a decrease in total demand for dairy products in the Russian market with a decrease in the purchasing power of the population, a change in the structure of consumption. An assessment of new opportunities and threats to the development of the industry was carried out: on the part of consumers — rising food prices, lower incomes; from the perspective of dairy producers — reduction in purchase prices, increased requirements for raw materials for the production of products with extended periods, labor shortages, the likely reduction in state support as a result of lower prices for hydrocarbons, attempts to regulate prices; on the part of processing enterprises — reduction of demand, reduction of prices for finished goods following world prices. The investment priorities in dairy cattle breeding and the main forms of improving state support for the industry, ensuring the effective adaptation of milk producers to new economic conditions, increasing the sustainability of the industry with increasing macroeconomic risks, are substantiated.

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Ukraine’s foreign trade in meat and meat products: trends and geographical aspects

Socio-Economic Problems of the Modern Period of Ukraine ◽

10.36818/2071-4653-2020-3-7 ◽

2020 ◽

pp. 48-52

Author(s):

Olena Lyakhovska

Keyword(s):

Small Businesses ◽

Structural Changes ◽

Raw Materials ◽

Meat Products ◽

World Market ◽

Poultry Meat ◽

Positive Development ◽

Meat Production ◽

Meat Industry ◽

Geographical Structure

Current trends in meat and meat products exports are considered. The dynamics and structural changes in meat exports by types and degree of processing (beef and veal, pork, poultry, finished meat products) are analyzed. The paper proves that in recent years, only meat and edible poultry by-products have taken a competitive position in the world market. Accordingly, the dynamics of exports of this type of meat is positive, and the share of these products in the structure of exported meat is the largest (86.09%). Possible threats to the growth of import dependence on certain types of meat products, in particular, chilled and frozen beef and veal, pork, are indicated. The rating of the largest countries-importers of meat and meat products (by types) from Ukraine is built, their share in the structure of meat exports is determined. Changes in the geographical structure of exports have been studied, which indicate a low level of diversification of the geography of exports of certain types of meat (pork, beef). At the same time, a positive development of the geographical structure of poultry meat exports (exported to more than 100 countries) was revealed. Problems of the export potential development in the meat industry of Ukraine are revealed. In particular, the problems of livestock development (outdated technologies and production techniques that reduce the efficiency and productivity; inefficiency of the organization of purchase and processing of meat, which leads to reduced breeding in small businesses and households; inefficiency of state incentives, etc.) and industrial meat production (lack of raw materials, obsolescence of fixed assets and production technologies, poorly developed logistics, etc.). The solution of these problems requires: a systematic approach, which will encourage the cooperation of agricultural and industrial production, cooperation with importers of meat products and protection of domestic producers in the domestic and international markets, creating a favorable investment and information environment that will promote the meat industry development.

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Anthocyanins: Chemical Properties and Health Benefits: A Review

Current Nutrition & Food Science ◽

10.2174/1573401317999210101150652 ◽

2021 ◽

Vol 17 ◽

Author(s):

Siraj Salman Mohammad ◽

Renata Oliveira Santos ◽

Maria Ivone Barbosa ◽

José Lucena Barbosa Junior

Keyword(s):

Raw Materials ◽

Health Benefits ◽

Chemical Properties ◽

Extraction Methods ◽

The Body ◽

Raw Material ◽

Extraction Properties ◽

Therapeutic Properties ◽

Food Processes ◽

Shed Light

: Anthocyanins are widely spread in different kinds of food, especially fruits and floral tissues, there is an extensive range of anthocyanin compounds reach more than 600 exist in nature. Anthocyanins can be used as antioxidants and raw material for several applications in food and pharmaceutical industry. Consequently, a plenty of studies about anthocyanins sources and extraction methods were reported. Furthermore, many studies about their stability, bioactive and therapeutic properties have been done. According to the body of work, we firstly worked to shed light on anthocyanin properties including chemical, antioxidant and extraction properties. Secondly, we reported the applications and health benefits of anthocyanin including the applications in food processes and anthocyanin characteristics as therapeutic and prophylactic compounds. We reviewed anticancer, anti-diabetic, anti-fatness, oxidative Stress and lipid decreasing and vasoprotective effects of anthocyanins. In conclusion, because the importance of phytochemicals and bioactive compounds the research is still continuing to find new anthocyanins from natural sources and invest them as raw materials in the pharmaceutical and nutrition applications.

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Environmentally Friendly Techniques and Their Comparison in the Extraction of Natural Antioxidants from Green Tea, Rosemary, Clove, and Oregano

Molecules ◽

10.3390/molecules26071869 ◽

2021 ◽

Vol 26 (7) ◽

pp. 1869

Author(s):

Mariel Calderón-Oliver ◽

Edith Ponce-Alquicira

Keyword(s):

Green Tea ◽

Electric Fields ◽

Antioxidant Activities ◽

Environmentally Friendly ◽

Natural Antioxidants ◽

Deep Eutectic Solvents ◽

Extraction Methods ◽

Extraction Techniques ◽

Health Trends ◽

Food Applications

Many current food and health trends demand the use of more ecological, sustainable, and environmentally friendly techniques for the extraction of bioactive compounds, including antioxidants. However, extraction yields and final antioxidant activities vary between sources and are highly influenced by the given extraction method and nature and ratio of the employed solvent, especially for total polyphenols, flavonoids, and anthocyanins, which are well recognized as natural antioxidants with food applications. This review focused on the most common extraction techniques and potential antioxidant activity in the food industry for various natural antioxidant sources, such as green tea, rosemary, clove, and oregano. Green extraction techniques have been proven to be far more efficient, environmentally friendly, and economical. In general, these techniques include the use of microwaves, ultrasound, high hydrostatic pressure, pulsed electric fields, enzymes, and deep eutectic solvents, among others. These extraction methods are described here, including their advantages, disadvantages, and applications.

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Extraction of Carotenoids from Pumpkin Peel and Pulp: Comparison between Innovative Green Extraction Technologies (Ultrasonic and Microwave-Assisted Extractions Using Corn Oil)

Foods ◽

10.3390/foods10040787 ◽

2021 ◽

Vol 10 (4) ◽

pp. 787

Author(s):

Minaxi Sharma ◽

Rajeev Bhat

Keyword(s):

Corn Oil ◽

Antioxidant Activities ◽

Food Product ◽

Extraction Techniques ◽

Protection Factor ◽

Total Carotenoids ◽

Green Extraction ◽

Microwave Assisted ◽

Potential Applications ◽

Conventional Extraction

Natural pigments improve aesthetic value as well as antioxidant potential of a food product. This study was designed to determine the effects of green extraction techniques on carotenoids, polyphenols and antioxidant activities of pulp and peel of two varieties of pumpkin (Cucurbita maxima). Innovative green extractions (IGE; Ultrasound and Microwave-Assisted Extractions) synergised with corn oil (used as green solvent) were compared with conventional extraction (CE; hexane/isopropyl alcohol; 60:40, v/v). Results showed total carotenoids to be almost double on employing IGE (PM2-UAE-peel = 38.03 ± 4.21; PM4-UAE-peel = 33.78 ± 1.76 µg/g) when compared to conventional extraction (PM2-CE-peel = 19.21 ± 4.39; PM4-CE-peel = 16.21 ± 2.52 µg/g). Polyphenolic contents ranged between 510.69 ± 5.50 and 588.68 ± 7.26 mg GAE/100 g of extract in IGE, compared with conventional extracts (269.50 ± 2.17 to 318.46 ± 6.60 mg GAE/100 g) and percent inhibition of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) ranging between 88.32 ± 1.51 and 93.53 ± 0.30% in IGE when compared with conventional extraction (50.61 ± 1.44 to 57.79 ± 2.09%). Further, oxidative stability of carotenoids extracts from IGE (protection factor = 1.59 ± 0.01 to 1.81 ± 0.05) were found to be significantly higher (p < 0.05) than conventional extracts. Based on results, this study supports the use of innovative green extraction techniques to obtain bioactive pigments like carotenoids. It is anticipated that results generated will find potential applications in food, pharmaceutical and cosmetic industries.

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