Synergistic antibacterial effect of Lepidium sativum and Coriandrum sativum against standard and drug resistant clinically isolated pathogenic bacteria

In present research we had selected two plant-Ziziphus mauritiana commonly known as Indian jujube (Ber) and Coriandrum sativum commonly known as coriander for the phytochemical screening and antimicrobial activity against Kliebsella pneumonia, Clostridium per fringes, Citrobacter freundii and Staphylococcus aureus bacteria. All the bacteria selected in this study are highly pathogenic and drug resistant. Phytochemical screening reveals that both the plants are rich in terms of phytoconstituents and potent against pathogenic bacteria.

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Antioxidant and Antibacterial Effects of Pollen Extracts on Human Multidrug-Resistant Pathogenic Bacteria

Journal of Food Quality ◽

10.1155/2021/5560182 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Meryem Bakour ◽

Hassan Laaroussi ◽

Driss Ousaaid ◽

Bouchra Oumokhtar ◽

Badiaa Lyoussi

Keyword(s):

Pathogenic Bacteria ◽

Quercus Ilex ◽

Antibacterial Effect ◽

Punica Granatum ◽

Coriandrum Sativum ◽

Bioactive Molecules ◽

Diffusion Method ◽

Citrus Aurantium ◽

Ruta Graveolens ◽

Centaurium Erythraea

The present work was designed to search the possible antibacterial effect of the ethanolic extract of pollens from six botanical origins, Punica granatum, Quercus ilex, Centaurium erythraea, Coriandrum sativum, Ruta graveolens, and Citrus aurantium, against multidrug pathogenic bacteria, Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Acinetobacter baumannii, Klebsiella pneumoniae, and Staphylococcus aureus. The content of phenolic compounds, flavones, and flavonols was measured. The antioxidant activities were evaluated using four assays: total antioxidant capacity, DPPH, ABTS, and reducing power. Antibacterial activity was studied using the agar disk diffusion method, and the MIC and MBC were determined. Results obtained showed a positive correlation between the antioxidant content of pollen extracts and the antibacterial capacity, Punica granatum and Quercus ilex pollen extracts were the most efficient against Gram-positive and Gram-negative bacteria, and Centaurium erythraea, Coriandrum sativum, and Ruta graveolens had a moderate effect, while Citrus aurantium had no antibacterial effect. It is concluded that pollens can be a good source of bioactive molecules that exhibit potent antioxidant effects and strong antibacterial activities.

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Multi-Drug Resistant Organisms Infection Impact on Patients Length of Stay in Respiratory Care Ward

Antibiotics ◽

10.3390/antibiotics10050608 ◽

2021 ◽

Vol 10 (5) ◽

pp. 608

Author(s):

Yi-Ping Chen ◽

Xian-Wen Tasi ◽

Ko Chang ◽

Xuan-Di Cao ◽

Jung-Ren Chen ◽

...

Keyword(s):

Pathogenic Bacteria ◽

Length Of Hospital Stay ◽

Selective Medium ◽

Respiratory Care ◽

Control Group ◽

Resistant Organism ◽

Case Group ◽

Drug Resistant ◽

Factors Affecting ◽

Care Ward

This study aimed to investigate the effects of multi-drug-resistant organism (MDRO) infection and other factors on the length of hospital stay (LOS) of patients in the respiratory care ward (RCW) of a regional hospital in Taiwan. In this retrospective study, we collected cases from MDRO-infected patients in the RCW from January 2016 to March 2020. The RCW comprises 13 beds in total. There were 106 infected patients, of which 42 were in the case group (infected with MDROs) and 64 were in the control group (not infected with MDROs). Clinical specimens were inoculated in a selective medium to isolate the pathogenic bacteria by standard procedures. The results showed the main factors affecting the LOS were: patients with MDRO infection, patients discharged from the RCW, and patients who underwent catheterization. The LOS of patients infected with MDROs was significantly longer than that of patients without MDRO infection (β = 0.55, 95% CI = 0.02–1.09), with the case group and the control group being 479.8 ± 546.5 and 307.3 ± 436.2 days, respectively. Infection with carbapenem-resistant Pseudomonas aeruginosa (CRPA) was associated with a longer LOS than other MDRO strains. These findings have important implications for infection control in RCW and in better tracking the health of patients.

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Synergetic effect of some plant essential oils with antibiotics against drug resistant pathogenic bacteria

10.36295/asro.2021.24448 ◽

2021 ◽

Vol 24 (04) ◽

Author(s):

Dr Mohsin A. Essa ◽

Ranaa W. Younus ◽

Mohammad M.Salih

Keyword(s):

Essential Oils ◽

Pathogenic Bacteria ◽

Synergetic Effect ◽

Drug Resistant ◽

Plant Essential Oils

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Comparative Evaluation of the Sterilization Effect of Six Kinds of Commercial Disinfectants against Four Pathogenic Bacteria

10.21203/rs.3.rs-652081/v1 ◽

2021 ◽

Author(s):

Wenxia Wang ◽

Xiaoting Liang ◽

Junxia Zheng ◽

Qi He

Keyword(s):

Sodium Hypochlorite ◽

Peracetic Acid ◽

Pathogenic Bacteria ◽

Antibacterial Effect ◽

Antibacterial Properties ◽

Water Soluble ◽

Absolute Ethanol ◽

Dilution Method ◽

Clostridium Sporogenes ◽

Sterilization Effect

Abstract In this work, we systematically investigate the sterilization effect of six kinds of commonly used commercial disinfectants, including the DuPont Virkon disinfectant, peracetic acid disinfectant, sodium hypochlorite, bromogeramine disinfectant, water-soluble allicin, and absolute ethanol, against the Escherichia coli, Staphylococcus aureus, Monilia albican and Clostridium sporogenes. The inhibition zone was used to qualitatively determine the antibacterial effects of the six disinfectants, and then the minimum two-fold dilution method was used to quantitatively determine the minimum inhibitory concentration and minimum bactericidal concentration of the six disinfectants on the four pathogens. The result illustrated that the antibacterial effect of peracetic acid disinfectant is the best, and clostridium sporogenes is the most sensitive to it, followed by bromogermine disinfectant, which can inhibit the four pathogenic bacteria at the concentration recommended by the manufacturer. The antibacterial effect of DuPont Virkon disinfectant, sodium hypochlorite, water-soluble allicin and absolute ethanol is not as good as expected, and cannot inhibit the four kinds of pathogenic bacteria at the recommended concentration. In summary, the antibacterial effect of peracetic acid disinfectant is the strongest, followed by the bromogermine disinfectant, DuPont Virkon disinfectant, sodium hypochlorite and water-soluble allicin. The absolute ethanol exhibits the worst antibacterial properties.

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Bakteri Simbion Gastropoda Pleuroploca trapesium Dari Perairan Ternate, Sebagai Alternatif Antibakteri MDR (Bacterial Symbiont Gastropoda Pleuroploca trapezium from Ternate, as Alternative Antibacterial MDR)

ILMU KELAUTAN Indonesian Journal of Marine Sciences ◽

10.14710/ik.ijms.19.1.55-62 ◽

2014 ◽

Vol 19 (1) ◽

pp. 55

Author(s):

Delianis Pringgenies ◽

Person Pesona Renta

Keyword(s):

16S Rdna ◽

Pathogenic Bacteria ◽

Dna Amplification ◽

Sensitivity Test ◽

Symbiotic Bacteria ◽

Drug Resistant ◽

16S Rdna Sequence Analysis ◽

Close Relationship ◽

Antibiotic Compounds ◽

Bacterial Sensitivity

Bakteri yang resisten terhadap beberapa jenis antibakteri ini dikenal dengan bakteri multi drug resistant (MDR).Untuk mengatasi permasalahan tersebut, perlu dilakukan pencarian senyawa antibiotik baru yang lebih efektif dan efisien dalam mengatasi permasalahan bakteri MDR. Penelitian bertujuan untuk mengetahui potensi bakteri yang bersimbiosis dengan gastropoda Pleuroploca trapezium sebagai sumber antibakteri MDR. Sampel Moluska dikoleksi dari perairan Ternate, Maluku. Tahapan penelitian meliputi isolasi bakteri, skrining bakteri simbion yang potensi sebagai anti bakteri MDR, uji antibakteri, isolasi bakteri patogen klinis MDR; uji sensitivitas anti-bakteri, ekstraksi, amplifikasi dan sekuensing DNA. Hasil 16S urutan r-DNA dianalisis dan diedit menggunakan program Genetix dan diikuti dengan analisis urutan 16S rDNA. Hasil menunjukkan bahwa terdapat 19 isolat bakteri dengan 5 bakteri aktif yang berasosiasi dengan Pleuroploca trapezium. Berdasarkan besarnya zona hambat yang dibentuk dan konsistensi munculnya zona hambatan, isolat terbaik adalah TPT 4.7. Isolat ini memiliki hubungan yang dekat dengan Paracoccus sp. MBIC4019 dengan homologi sebesar 95% yang menunjukkan kekerabatan ditingkat genus. Hasil penelitian ini memberikan harapan adanya potensi besar sebagai bahan antibakteri baru. Kata kunci: antibakteri, simbion, Pleuroploca trapezium, multi drugs resistantThe bacteria resistant to some antibiotics are known as multi drug resistant (MDR). To overcome the problem, it is needed to search for a new antibiotic compounds more effectively and efficiently. This study aims to identify potential from symbionts of Pleuroploca trapezium as a source of antibacteria MDR and identifying the bacteria that were active against the MDR. Samples were collected from Ternate, Maluku. Isolation of symbiotic bacteria, screening for bacteria which producing secondary metabolites as anti-MDR bacteria, antibacterial test, isolation of clinical pathogenic bacteria of MDR. Conducting anti-bacterial sensitivity test, sensitivity test for antibacterial, DNA exctraction, DNA amplification based on PCR method, DNA sequencing. Result of 16S r-DNA sequence was then analyzed and edited using GENETYX program and followed by 16S rDNA sequence analysis. Screening of bacteria associated with P. trapezium resulted in 19 isolates with 5 active bacteria. Based on the size of the zone forming and the consistency of zone, so the best isolate is TPT 4.7. The identification shows that TPT 4.7 has a close relationship with the Paracoccus sp. MBIC4019 with homologi of 95%, which shows the relationship at the genus level. Its suggest that these results are very promising as a new antibacterial material. Keywords: antibacterial, symbiotic bacteria, Pleuroploca trapezium, multi drugs resistant

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Antibacterial Effect of Carvacrol and Coconut Oil on Selected Pathogenic Bacteria

Scientia Agriculturae Bohemica ◽

10.2478/sab-2018-0008 ◽

2018 ◽

Vol 49 (1) ◽

pp. 46-52 ◽

Cited By ~ 1

Author(s):

M. Božik ◽

P. Hovorková ◽

P. Klouček

Keyword(s):

Essential Oils ◽

Lauric Acid ◽

Pathogenic Bacteria ◽

Antimicrobial Agents ◽

Antibacterial Effect ◽

Cocos Nucifera ◽

Coconut Oil ◽

High Concentration ◽

Enterococcus Cecorum ◽

Antibacterial Potential

AbstractEssential oils play a prominent role as flavouring agents and fragrances in the food and perfume industries. Carvacrol is a major component of various essential oils, such as oregano and thyme oils, and is responsible for their antimicrobial activity. Lauric acid is a medium-chain fatty acid (MCFA) with a high antibacterial potential. Both carvacrol and MCFAs have been used empirically as antimicrobial agents. Here, we tested the inhibitory properties of carvacrol and coconut (Cocos nuciferaL.) oil containing a high percentage of MCFAs against 5 harmful bacterial pathogens:Escherichia coli, SalmonellaEnteritidis,Staphylococcus aureus, Listeria monocytogenes, andEnterococcus cecorum. Gas chromatography (GC-FID) analysis of coconut oil showed a high concentration of lauric acid (41%). Microdilution antimicrobial assays showed that the combination of carvacrol and coconut oil had a stronger antibacterial effect against all tested bacteria than both agents separately. We conclude that carvacrol could significantly improve the antibacterial effect of coconut oil.

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Ribosome Rescue Pathways in Bacteria

Frontiers in Microbiology ◽

10.3389/fmicb.2021.652980 ◽

2021 ◽

Vol 12 ◽

Author(s):

Claudia Müller ◽

Caillan Crowe-McAuliffe ◽

Daniel N. Wilson

Keyword(s):

Messenger Rna ◽

Pathogenic Bacteria ◽

Antimicrobial Agents ◽

Polypeptide Chain ◽

Drug Resistant ◽

Bacterial Survival ◽

Gram Positive Bacteria ◽

Small Protein B ◽

Recent Developments ◽

Rescue System

Ribosomes that become stalled on truncated or damaged mRNAs during protein synthesis must be rescued for the cell to survive. Bacteria have evolved a diverse array of rescue pathways to remove the stalled ribosomes from the aberrant mRNA and return them to the free pool of actively translating ribosomes. In addition, some of these pathways target the damaged mRNA and the incomplete nascent polypeptide chain for degradation. This review highlights the recent developments in our mechanistic understanding of bacterial ribosomal rescue systems, including drop-off, trans-translation mediated by transfer-messenger RNA and small protein B, ribosome rescue by the alternative rescue factors ArfA and ArfB, as well as Bacillus ribosome rescue factor A, an additional rescue system found in some Gram-positive bacteria, such as Bacillus subtilis. Finally, we discuss the recent findings of ribosome-associated quality control in particular bacterial lineages mediated by RqcH and RqcP. The importance of rescue pathways for bacterial survival suggests they may represent novel targets for the development of new antimicrobial agents against multi-drug resistant pathogenic bacteria.

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Efflux Pump Inhibition and Resistance Modulation in Mycobacterium smegmatis by Peucedanum ostruthium and Its Coumarins

Antibiotics ◽

10.3390/antibiotics10091075 ◽

2021 ◽

Vol 10 (9) ◽

pp. 1075

Author(s):

Katarina Šimunović ◽

Julia Solnier ◽

Fabian Alperth ◽

Olaf Kunert ◽

Sonja Smole Smole Možina ◽

...

Keyword(s):

Antibiotic Resistance ◽

Mycobacterium Smegmatis ◽

Antibacterial Effect ◽

Efflux Pump ◽

Antimicrobial Effect ◽

Drug Resistant ◽

Health Crisis ◽

Efflux Pump Inhibition ◽

Resistance Modulation ◽

Distinct Role

Antibiotic resistance is a growing problem and may become the next major global health crisis if no timely actions are taken. Mycobacterial infections are widespread and, due to antibiotic resistance, also hard to treat and a major cause of mortality. Natural compounds have the potential to increase antibiotic effectiveness due to their resistance modulatory and antimicrobial effects. In this study, Peucedanum ostruthium extracts, fractions, and isolated compounds were investigated regarding their antimicrobial and resistance-modulatory effects as well as efflux pump inhibition in Mycobacterium smegmatis. P. ostruthium extracts were found to have anti-mycobacterial potential and resistance modulating effects on ethidium bromide activity. The major antibacterial effect was attributed to ostruthin, and we found that the more lipophilic the substrate, the greater the antimicrobial effect. Imperatorin caused potent modulatory effects by interfering with the action of the major LfrA efflux pump in M. smegmatis. The plant P. ostruthuim has a complex effect on M. smegmatis, including antibacterial, efflux pump inhibition, resistance modulation, and membrane permeabilization, and its major constituents, ostruthin and imperatorin, have a distinct role in these effects. This makes P. ostruthium and its coumarins promising therapeutics to consider in the fight against drug-resistant mycobacteria.

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The Identification of Multidrug Resistant Microorganisms Including Bergyella Zoohelcum Acquired from the Skin/Prosthetic Interface of Amputees and Their Susceptibility to Medihoney™ and Garlic Extract (Allicin)

10.21203/rs.3.rs-1026595/v1 ◽

2021 ◽

Author(s):

Ruby Harsent ◽

Paul Smith ◽

James Arthur Blaxland ◽

Neil Rushmere

Keyword(s):

Pathogenic Bacteria ◽

Micrococcus Luteus ◽

Multidrug Resistant ◽

Garlic Extract ◽

Identification System ◽

Drug Resistant ◽

Manuka Honey ◽

Prosthetic Devices ◽

Microbiological Methods ◽

Oxidase Test

Abstract BackgroundUsers of prosthetic devices face the accumulation of potentially drug-resistant pathogenic bacteria on the skin/prosthesis interface. In this study, we took surface swabs of the skin/prosthesis interface of eleven disabled athletes to identify microorganisms present. In addition to determining their antimicrobial resistance profile, we assessed their sensitivity to Manuka honey and Garlic extract (allicin) MethodsEleven volunteers were directed to swab the skin at the skin/prosthesis interface. After initial isolation of microorganisms we employed the following general microbiological methods; Gram stain, Catalase test, Oxidase test, lactose fermenting capability, haemolytic capability, Staphaurex, mannitol fermenting capability, Streptex; API Staph, 20E, Candida, and BBL crystal identification system tests. Once identified, isolates were analysed for their sensitivity to penicillin, erythromycin. ampicillin, vancomycin, ceftazidime, ciprofloxacin, gentamicin and colistin-sulphate. Isolates were also analysed for their sensitivity to allicin (Garlic Extract (GE)) and Manuka honey (Medihoney™) (MH). ResultsEleven isolates were identified, Bacillus cereus, Staphylococcus haemolyticus, Staphylococcus aureus, Micrococcus luteus, Pseudomonas oryzihabitans, Micrococcus spp., Bacillus subtilis, Group D Streptococcus, Pantoea spp., Enterobacter cloacae and Bergyella zoohelcum. All Gram-positive organisms were resistant to 1.5 units of penicillin and 10 μg of ampicillin, and two Gram-negatives Pseudomonas oryzihabitans and Bergyella zoohelcum were resistant to 10 μg ceftazidime, whilst Bergyella zoohelcum, was also resistant to 10 μg of gentamicin. In comparison, all organisms were sensitive to Manuka honey and nine sensitive to Allicin. ConclusionsThis study highlights the prevalence of uncommon drug resistant microorganisms on the skin within a vulnerable population, highlighting the potential for MH or GE intervention.

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