Evaluation of Acute Oral Toxicity and Mast Cell Degranulation of an aqueous ethanolic extract of Tritium aestivum Linn.

2018 ◽  
Vol 11 (2) ◽  
pp. 643
Author(s):  
Vibhor K. Jain ◽  
Dheeraj Ahirwar ◽  
Bindu Jain ◽  
Bharati Ahirwar
Keyword(s):  
Mast Cell ◽  
Ethanolic Extract ◽  
Mast Cell Degranulation ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Aqueous Ethanolic Extract

scholarly journals Acute oral toxicity evaluation of aqueous ethanolic extract of Saccharum munja Roxb. roots in albino mice as per OECD 425 TG

2017 ◽  
Vol 4 ◽  
pp. 580-585 ◽  
Author(s):  
Uzma Saleem ◽  
Sadia Amin ◽  
Bashir Ahmad ◽  
Haroon Azeem ◽  
Fareeha Anwar ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Acute Oral Toxicity ◽  
Toxicity Evaluation ◽  
Oral Toxicity ◽  
Aqueous Ethanolic Extract ◽  
Albino Mice ◽  
Saccharum Munja

scholarly journals ACUTE ORAL TOXICITY STUDY OF ETHANOLIC EXTRACT OF ACTINOSCIRPUS GROSSUS (L.F.) GOETGH. AND D.A. SIMPSON

2018 ◽  
Vol 11 (7) ◽  
pp. 321
Author(s):  
Savin Chanthala Ganapathi ◽  
Rajendra Holla ◽  
Shivaraja Shankara Ym ◽  
Ravi Mundugaru
Keyword(s):  
Body Weight ◽  
Lethal Dose ◽  
Ethanolic Extract ◽  
Female Rats ◽  
Test Substance ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Healthy Female ◽  
Ld50 Value ◽  
Toxic Potential

Objective: To study the acute oral toxicity of ethanolic extract of Actinoscirpus grossus (L.f.) Goetgh. and D.A. Simpson in Wistar albino rats.Methods: Ethanolic extract of the plant was assessed for single dose acute toxicity by employing Organisation for Economic Co-Operation and Development(OECD) guidelines 425 using Acute Oral Toxicity(AOT) software. The dosed (up or down as per the requirement) rats were observed for 14 days for general appearance, behavior, mortality, and necropsy. A total of 5 healthy female rats of body weight 225±25 g were used.Results: The test substance did not produce any mortality up to the dose of 2000 mg/kg per oral.Conclusion: Test substance is without any toxic potential even at the dose of 2000 mg/kg in animals and the Lethal Dose (LD50) value of A. grossus (L.f.) Goetgh. and D.A. Simpson was found to be more than 2000 mg/kg body weight.

scholarly journals Acute oral toxicity evaluation of Andrographis paniculata-standardized first true leaf ethanolic extract

2019 ◽  
Vol 6 ◽  
pp. 426-430 ◽  
Author(s):  
Luksamee Worasuttayangkurn ◽  
Watanyoo Nakareangrit ◽  
Jackapun Kwangjai ◽  
Pishyaporn Sritangos ◽  
Nanthanit Pholphana ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Andrographis Paniculata ◽  
Acute Oral Toxicity ◽  
Toxicity Evaluation ◽  
Oral Toxicity ◽  
True Leaf

scholarly journals Study on Acute Oral Toxicity of Ethanolic Extract of Annonasquamosa Leaves in Mice (Musmusculus)

2018 ◽  
Vol 1 (1) ◽  
pp. 56-63
Author(s):  
Khairunnisa ◽  
Andini Dita Utami ◽  
Marianne
Keyword(s):  
Ethanolic Extract ◽  
Fixed Dose ◽  
Control Group ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Hydropic Degeneration ◽  
Treatment Groups ◽  
Dose Method ◽  
Liver And Kidney ◽  
Tubular Dilatation

Abstract. The aims of this study were to determine the potential for acute oral toxicity of ethanolic extract of A.squamosa leaves with LD50 and the histopathological changes in liver and kidney of mice.This research used experimental method as per fixed dose method. The number of animals used in this research were 20 female mice. The study was divided into 2 steps, there were sighting and main studies. The control group was given Na-CMC 0.5%, the treatment groups were given ethanolic extract of A.squamosa leaves with doses of 5, 50, 300,  2,000 and 5,000 mg/kg bw. The results showed that the ethanolic extract of A.squamosa leaves with doses of 2,000 and 5,000 mg/kg bw did not show any toxicity signs. At a dose of 5,000 mg/kg bw caused hydropic degeneration, necrosis hepatocyte, glomerular atrophy, and tubular dilatation.  There was no mortality was observed.It was estimated that LD50 of ethanolic extract of A.squamosa leaves was higher than 5,000 mg/kg bw and the extract were practically non-toxic. Keywords: Acute Toxicity,  Annona squamosa, Ethanolic Extract

scholarly journals Evaluation of the Antinociceptive Activity and Acute Oral Toxicity of Standardized Ethanolic Extract of the Rhizome of Curcuma xanthorrhiza Roxb

Molecules ◽  
2010 ◽  
Vol 15 (4) ◽  
pp. 2925-2934 ◽  
Author(s):  
Sutha Devaraj ◽  
Azadeh Sabetghadam Esfahani ◽  
Sabariah Ismail ◽  
Surash Ramanathan ◽  
Mun Fei Yam
Keyword(s):  
Ethanolic Extract ◽  
Antinociceptive Activity ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Curcuma Xanthorrhiza

Clinacanthus nutans Aqueous Extract But Not Ethanolic Extract Inhibits the Release of Preformed Mediators in In Vitro Model of IgE-Mediated Mast Cell Degranulation

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Audrey Siew Foong Kow ◽  
Lai Yen Fong ◽  
Leng Wei Khoo ◽  
Ji Wei Tan ◽  
Khozirah Shaari ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Aqueous Extract ◽  
Inflammatory Mediators ◽  
Scientific Evidence ◽  
Ethanolic Extract ◽  
Mast Cell Degranulation ◽  
Traditional Use ◽  
Medicinal Uses

C. nutans (Burm. f.) Lindau is a shrub that is commonly found in South East Asia, particularly in Malaysia, Thailand and Indonesia. This plant has diverse and potential medicinal uses in traditional herbal medicine for treating many diseases which include skin rashes. Skin rash is one of the symptoms that primarily occur in people with allergies. Till date, there has been no scientific evidence to support this traditional use of C. nutans as well as its potential of possessing anti-allergic properties. IgE and mast cells play an important role in the pathogenesis of allergy. Upon cross-linking of allergen to IgE antibodies bound to FcεRI on mast cells, pro-inflammatory mediators will be released as a result of degranulation. The release of pro-inflammatory mediators by mast cells activated by allergen in the skin causes large, itchy, red swellings of the skin. This study aimed to identify the most active C. nutans extract in inhibiting in vitro IgE-mast cell degranulation. Rat basophilic leukaemic cells (RBL-2H3) were pre-sensitised with IgE, pre-treated with C. nutans ethanolic (100%, 70% and 50%) or aqueous extracts and challenged with dinitrophenyl-bovine serum albumin (DNP-BSA) accordingly to analyse the release of β-hexosaminidase, histamine, interleukin (IL)-4 and tumour necrosis factor (TNF)-α. It was found that C. nutans aqueous extract was the most active extract compared to the ethanolic extracts as it was able to inhibit the release of β-hexosaminidase and histamine at concentrations of 5 mg/ml and above.

scholarly journals ACUTE TOXICITY EVALUATION OF ETHANOLIC EXTRACT OF ARISTOLOCHIA ALBIDA DUCH. LEAVES ON WISTAR RATS LIVER AND KIDNEY FUNCTIONS

2017 ◽  
Vol 9 (7) ◽  
pp. 35 ◽  
Author(s):  
Felix F. D. Guinnin ◽  
Jean Robert Klotoe ◽  
Jean Marc Ategbo
Keyword(s):  
Biochemical Parameters ◽  
Statistical Difference ◽  
Clinical Signs ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Mean Cell Volume ◽  
Limit Test ◽  
Significant Difference

Objective: Aristolochia albida Duch is frequently used by alternative medicine to treat some pathologies like hepatitis. Our main objective was to evaluate the acute oral toxicity of the ethanolic extract of this plant.Methods: Exploratory tests for acute oral toxicity are performed in vivo on Wistar albino rats in a limit test of 2000 mg/kg for 14 d in accordance with the OECD Guidelines 423. The clinical signs were observed every day, followed by measurement of body weight change, the haematological and biochemical examinations were executed and statistical analysis was performed. Results: The various clinical signs observed after administration and for 14 d were recorded and no mortality was observed. With the exception of white blood cells, mean cell volume and platelets with statistically significant difference in control (p<0.05), all the haematological parameters showed an insignificant statistical difference to the control ratio (p>0.05). For biochemical parameters, except blood glucose and total protein of control batches, which show a significant statistical difference after 14 d (p>0.05), all the biochemical parameters show the statistically insignificant difference for test and controls batches (p>0.05) as well as the weight variation of the animals.Conclusion: The ethanolic extract of the leaves of A. albida Duch (EEAr) had not toxic effect on the biochemical and hematological parameters studied at a dose of 2000 mg/kg. The lethal dose is therefore over 2000 mg/kg.

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