Effect of Ethanol Extract from Purple Eggplant Skin (Solanum melongena L) On Blood Glucose Levels and Pancreatic β Cells Regeneration on White Rats Male Hypercholesterolemia-Diabetic

2019 ◽  
Vol 12 (6) ◽  
pp. 2936
Author(s):  
Joni Tandi ◽  
Rama Danthy ◽  
Purwaningsih ◽  
Hadi Kuncoro
Keyword(s):  
Blood Glucose ◽  
Solanum Melongena ◽  
Ethanol Extract ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

scholarly journals UJI ANTIDIABETIK EKSTRAK ETANOL BUNGA PEPAYA (Carica papaya L.) TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 160
Author(s):  
Adinda Fransisca Pongoh ◽  
Edwin De Queljoe ◽  
Henki Rotinsulu
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Carica Papaya ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Control Group ◽  
Homogeneity Test ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

ABSTRACT This study aims to determine the antidiabetic activity of papaya flower ethanol extract (Carica papaya L.) against male white rats (Rattus norvegicus) induced by alloxan. This research is experimental. Fifteen rats were divided into 5 treatment groups, each group consisted of 3 rats. The first group was the negative control group given Aquades, the second group was positive control given Glibenklamid, the three groups were 200 mg dose variation groups, four groups were 400 mg dose variations, and the five groups were 800 mg dose variations. Previously, rats were examined fasting blood glucose levels, then mice were induced by an alloxan dose of 120 mg / kgBW intraperitoneally. On the 3rd day blood glucose levels were examined and then treated according to groups for 7 days, measurement of blood glucose levels after the treatment was carried out once every 3 days namely day 3, day 7, and day 10. Data obtained were then analyzed statistically using SPSS , including normality test (Shapiro-Wilk), homogeneity test (Levene), ANOVA test (One way). The results of this study indicate that Papaya Flower (Carica papaya L.) Ethanol Extract can provide the best blood glucose level reduction effect at a dose of 800 mg. Keywords : Antidiabetic, Papaya Flower Extract (Carica papaya L.), Diabettes Mellitus, Male White Rat (Rattus norvegicus), Aloxan.  ABSTRAK Penelitian ini bertujuan untuk mengetahui efektivitas Antidiabetik Ekstrak Etanol Bunga Pepaya (Carica papaya L.) Terhadap Tikus Putih Jantan (Rattus norvegicus) Yang Diinduksi Aloksan. Penelitian ini bersifat eksperimental. Sebanyak 15 ekor tikus dibagi ke dalam 5 kelompok perlakuan masing- masing kelompok terdiri dari 3 ekor tikus. Kelompok pertama kelompok Kontrol negatif yang diberikan Aquades, Kelompok kedua Kontrol Positif yang diberikan Glibenklamid, kelompok tiga kelompok variasi dosis 200 mg, kelompok empat kelompok variasi dosis 400 mg, dan kelompok lima kelompok variasi dosis 800 mg. Sebelumnya tikus dilakukan pemeriksaan kadar glukosa darah puasa, selanjutnya tikus diinduksi Aloksan dosis 120 mg/kgBB secara intraperitoneal. Pada hari ke 3 diperiksa kadar Glukosa darah kemudian diberikan perlakuan sesuai kelompok selama 7 hari, pengukuran kadar glukosa darah setelah perlakuan dilakukan 3 hari sekali yaitu hari ke 3, hari ke 7, dan hari ke 10. Data yang diperoleh kemudian dianalisis secara statistik menggunakan SPSS, meliputi uji normalitas (Shapiro-Wilk), uji homogenitas (Levene), uji ANOVA (One way). Hasil penelitian ini menunjukkan bahwa Ekstrak Etanol Bunga Pepaya (Carica papaya L.)  dapat memberikan efek penurunan kadar glukosa darah yang paling baik yaitu pada dosis 800 mg. Kata kunci : Antidiabetik, Ekstrak Bunga pepaya (Carica papaya L.), Diabettes Mellitus, TikusPutih Jantan (Rattus norvegicus), Aloksan.

The Effect of Ethanol Extract of Guava Leaf on Hypercholesterolemia-Diabetes of White Rats Male Induced by High Fat Feed and Streptozotocin

2021 ◽  
Vol 7 (2) ◽  
pp. 177-187
Author(s):  
Joni Tandi ◽  
Franciska Diana Pratiwi ◽  
Surisna Surisna ◽  
Niluh Puspita Dewi ◽  
Magfirah Magfirah
Keyword(s):  
Blood Glucose ◽  
Effective Dose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Group I ◽  
Guava Leaf

This study aims to determine the effect of guava leaf extract (Psidiium guajava L) on hypercholesterolemia-diabetes and the effective dose for reducing cholesterol and blood glucose levels. This study is a laboratory experimental study using 30 rats divided into 6 test animals. the treatment group, each group consisted of 5 rats. Group I was normal control, group II negative control was given Na-CMC suspension, group III positive control was given simvastatin group IV dose 150 mg / kg BW, group V dose 250 mg / kg BW and group VI dose 350 mg / kg BW. The results showed that the ethanol extract of guava leaves had an effect on reducing blood glucose levels of male white rats with an effective dose of 250mg / kg BW with an average reduction of 119 mg/dL and effectively reduce cholesterol levels at a dose of 150 mg /kg BW with an average reduction of 28,33 mg/dL.

scholarly journals Skrining Fitokimia dan Potensi Antidiabetes Ekstrak Etanol Herba Ciplukan (Physalis Angulata L) pada Tikus Putih (Rattus Novergicus) yang Diinduksi Aloksan

2019 ◽  
Vol 4 (3) ◽  
pp. 98-107
Author(s):  
Hendra Pratama Maliangkay ◽  
Rolef Rumondor ◽  
Mynia Kantohe
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Negative Control ◽  
Glucose Measurement ◽  
Percentage Decrease ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Physalis Angulata

The purpose of this study to determine the anti-diabetic potential of ciplukan herbal ethanol extract (Physalis angulata L) in white rats (Rattus norvegicus) induced alloxan given intraperitoneally and comparing its effectiveness with glibenclamide. This study uses 5 groups namely giving aquadest as normal control, giving alloxan as a negative control, giving alloxan and glibenclamide as a positive control and the ethanol extract of ciplukan herbal with a dose of 150 mg/kg BB and 300 mg/kg BB. Blood glucose measurement performed on day 0 (three days after induction), day 7, and day 14. The results showed that ethanol extract of ciplukan herbal (Physalis angulata L) can lower blood glucose levekls. A dose of 150 mg/kg BB have percentage decrease in blood glucose levels on day 7 and day 14 respectively by 14.1%and 70.5%. dose of 300 mg/kg BB have a percentage decrease in blood glucose levels on day 7 and day 14 respectively by 70.5%.and 69.5%. a dose of 150 mg/kg BB is more effective as an anti-diabetic compared with a dose of 300 mg/kg BB. Ethanol extract of ciplukan herbal (Physalis angulata L) also has an activity to repair damaged pancreatic-β cells in rats (Rattus norvegicus) induced by alloxan.

scholarly journals Potensi Rumput Laut (Eucheuma cottonii J.Agardh) Terhadap Nefropati Diabetik Tikus Putih Jantan (Rattus norvegicus)

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Joni Tandi ◽  
Niluh Puspita Dewi ◽  
Resky Chandra Wirawan ◽  
Megawati R. Surat
Keyword(s):  
Blood Glucose ◽  
Kidney Tissue ◽  
Ethanol Extract ◽  
Seaweed Extract ◽  
Average Value ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Significant Difference ◽  
Eucheuma Cottonii

ABSTRACT   This study aims to determine the effect of a multilevel dose of ethanol extract of seaweed (Eucheuma cottonii J. Agardh) on the decreasing blood glucose levels and kidney tissue regeneration of hypercholesterolemia-diabetic male white rats. 24 rats were divided into 6 groups, namely groups 1-3 (normal control, negative control, and positive control/Glibenclamide, respectively) and groups 3-6 (seaweed extract doses of 100, 200, and 400 mg/kg BW per oral, respectively). Rats were induced by high fat diet and Streptozotocin. Histopathological picture of the kidney was observed by HE staining using 400 magnifications. Data scoring of the level of kidney damage were analyzed by One Way ANOVA at a 95% confidence level and a Least Significant Difference (LSD) test was conducted. Seaweed ethanol extract at a dose of 100 mg/kg BW was effective in reducing blood glucose levels with an average value of 122.25 mg/dL and effective in regenerating the kidney tissue of male white rats with an average value of damage of 1.9. Therefore, seaweed extract has potency to be developed and further investigated as an antidiabetic agent.

scholarly journals Test of the Potential of Ethanol Extracts, Simplician Medium and Forest Umbi Juice (Eleutherine bulbosa (Factory) Urb.) against Blood Glucose Levels of Rats, and Histopatology Ratkreas Rat (Rattus norvegicus) Hypercolesterolemia Model Diabetic

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Joni Tandi ◽  
Tien Wahyu Handayani ◽  
Ni Wayan Ika Purwasih
Keyword(s):  
Blood Glucose ◽  
Test Group ◽  
Ethanol Extract ◽  
Positive Control ◽  
Pancreatic Tissue ◽  
Glucose Levels ◽  
Onion Bulbs ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Group I

This study aims to examine the presence or absence of secondary metabolite compounds in ethanol extract, simplicia steeping and forest onion tuber juice, in male glucose parameters and pancreatic histopathology. Using test animals as many as 30 male white rats divided into 6 groups and each group consisted of 5 rats with group I details as normal controls, group II as negative controls given 0.5% Na-CMC suspension, group III as positive control who were given Metformin 45 mg / kg BB and IV, V, VI as the test group were given ethanol extract, simplicia extract and forest onion tuber juice with each dose of 20 g / kg BW. The results showed that there were secondary metabolites in the ethanol extract of forest onion tubers, namely flavonoids, saponins, alkaloids, steroids and tannins, giving ethanol extract of forest onion bulbs had the effect of reducing blood glucose levels in white mice with a dose of 20g / kgBW with an average of 132 and ethanol extract of forest onion bulbs at a dose of 20g / kg BB with an average of 0.5 can regenerate pancreatic tissue.

scholarly journals EFEK ANTIHIPERGLIKEMIK EKSTRAK ETANOL DAUN BENALU KERSEN (Dendrophthoe pentandra (L.) Miq) PADA TIKUS PUTIH JANTAN YANG DIINDUKSI ALOKSAN

2021 ◽  
Vol 6 (2) ◽  
pp. 168-175
Author(s):  
Adiyatma Putra Mahardika ◽  
◽  
Sadakata Sinulingga ◽  
Medina Athiah ◽  
Subandrate Subandrate ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Homogeneity Test ◽  
Test Design ◽  
Glucose Levels ◽  
White Rats ◽  
Normality Test ◽  
Blood Glucose Levels ◽  
Experimental Laboratory

This study aims to determine the effect of lowering blood glucose. The study was conducted in an experimental laboratory in vivo with one group randomized prepost-test design. The extract was made through the gradual maceration process of Benalu Kersen leaf simplicia with ethanol solvent. The extract was divided into three groups with doses of 65 mg / kg, 130 mg / kg, and 260 mg / kg of body weight. The sample used was 9 male white rats (Rattus norvegicus) which were randomly divided into three groups. Blood glucose levels were measured before induction, after induction, after 7 days, and after 14 days of giving the extract. Data analysis was performed in the form of homogeneity test, normality test, paired t-test using Excel and SPSS programs. There was significant decrease in blood glucose levels on day 0 to day 7 in dose II group. The largest percentage reduction in blood glucose levels was shown in dose II group, namely 32.6%, followed by dose I group at 18.89%. The ethanol extract of Benalu kersen leaves did have an effect of lowering blood glucose levels on alloxan-induced male white rats.

scholarly journals UJI AKTIVITAS ANTIHIPERGLIKEMIA EKSTRAK ETANOL 70% Echinometra mathaei PADA TIKUS PUTIH JANTAN GALUR WISTAR YANG DIINDUKSI ALOKSAN

2021 ◽  
Vol 2 (1) ◽  
pp. 29
Author(s):  
Angelica Kresnamurti ◽  
Nana Angelica ◽  
Farizah Izazi
Keyword(s):  
Blood Glucose ◽  
Sea Urchin ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Standard Group ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Echinometra Mathaei

Abstract : Sea urchin (Echinometra mathaei) contains a compound of Echinochrome A, which is a polyhydroxy naphthoquinone that is known to be potential antioxidant. The antioxidant effect correlated with the antidiabetic activity that associated with oxidative stress conditions. The aim of this study was to determine the effect of 70% Ethanol Extract of sea urchin (Echinometra mathaei) on blood glucose levels in alloxan induced rats. This research was an experimental study using 30 male white rats, divided into 6 groups, i.e the negative control group (normal rat were only given CMC Na 0,5%), Positive control group (hyperglycemic rats were only treated with CMC Na 0,5%), metformin group as a refference standard group (hyperglycemic rats that treated with metformin) and the treatment group (hyperglycemic rats were treated with ethanol extract 70% of Echinometra mathaei dose of 100 mg/kgBW, 200 mg/kgBW and 400 mg/kgBW). Alloxan monohidrat 120 mg/KgBW was used as diabetogen agent. The parameters examined in this study were blood glucose levels of rats after 7 days of treatment. The observational data were analyzed using the One Way ANOVA (P < 0.05) and continued with LSD. The results of this study showed that all doses of ethanol extract significantly reduced blood glucose levels in rats compared to the control group. The most effective dose in reducing blood glucose levels and compared to metformin was 400 mg / kgBW. Key word: alloxan induced, Echinometra mathaei, ethanol 70%, hiperglycemia, marine product.

scholarly journals THE EFFECT OF AMINO ACID COMPOUNDS IN FERMENTED SOYBEANS AGAINST FIBROBLAST GROWTH FACTOR IN MICE PANCREATIC β-CELLS FIGURES

2018 ◽  
Vol 11 (9) ◽  
pp. 157
Author(s):  
Surya Dharma ◽  
Dedy Almasdy ◽  
Dwisari Dillasamola ◽  
Roslinda Rasyid ◽  
Dianty Dwi Wahyuni ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Fibroblast Growth ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Experimental Animals ◽  
Glucose Levels ◽  
Blood Glucose Levels

Objective: The aim of this study is to determine the effect of the amino acid compound in fermented soybeans against fibroblast growth factor (FGF) in mice’s pancreatic β-cell figures. This study was also done to know the effect of given combination of fertilized egg whites powder with tempe (Indonesian conventional food and the fermentation product of soybeans fermentation) in a dose of 7250 mg/kg.Methods: All of the experimental animals pancreases were designed to be damaged by given alloxan in a dose of 150 mg/kg, except for the negative control group without anything given. The experimental animals were divided into 7 groups which consist of the negative control group, positive control group, and the rests 5 group of FGF test preparation in dosages of 100; 140; 200; 300; and 425 mg/kg combined with fermented soybeans in a dose 7250 mg/kg. This experiment was conducted for 21 days, observed at 7th, 14th, and 21st day. The data analysis used is a statistical test of one-way analysis of variance (ANOVA) and two-way ANOVA.Results: The result showed a significant decrease in blood glucose levels (p<0.05) in mice for all treatments when compared with positive controls. From the result of the histopathologic examination, pancreatic β-cells improve utterly close to the control condition (-). In the qualitative immunohistochemical examination, there was a difference in the stained pancreatic β-cells marked by yellow density. Meanwhile, the quantitative observation did not show any improvement against normal condition control (-) (p<0.05).Conclusion: The combination of egg whites and fermented soybean significantly decreased the blood glucose levels, and the occurrence of the Langerhans island cells was nearly normal.

scholarly journals Somatostatin analogues for the treatment of hyperinsulinaemic hypoglycaemia

2020 ◽  
Vol 11 ◽  
pp. 204201882096506
Author(s):  
Basma Haris ◽  
Saras Saraswathi ◽  
Khalid Hussain
Keyword(s):  
Insulin Secretion ◽  
Blood Glucose ◽  
Glucose Metabolism ◽  
Therapeutic Effect ◽  
Glucagon Secretion ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Hyperinsulinaemic Hypoglycaemia ◽  
Glucose Levels ◽  
Blood Glucose Levels

Hyperinsulinaemic hypoglycaemia (HH) is a biochemical finding of low blood glucose levels due to the dysregulation of insulin secretion from pancreatic β-cells. Under normal physiological conditions, glucose metabolism is coupled to β-cell insulin secretion so that blood glucose levels are maintained within the physiological range of 3.5–5.5 mmol/L. However, in HH this coupling of glucose metabolism to insulin secretion is perturbed so that insulin secretion becomes unregulated. HH typically occurs in the neonatal, infancy and childhood periods and can be due to many different causes. Adults can also present with HH but the causes in adults tend to be different. Somatostatin (SST) is a peptide hormone that is released by the delta cells (δ-cells) in the pancreas. It binds to G protein-coupled SST receptors to regulate a variety of location-specific and selective functions such as hormone inhibition, neurotransmission and cell proliferation. SST plays a potent role in the regulation of both insulin and glucagon secretion in response to changes in glucose levels by negative feedback mechanism. The half-life of SST is only 1–3 min due to quick degradation by peptidases in plasma and tissues. Thus, a direct continuous intravenous or subcutaneous infusion is required to achieve the therapeutic effect. These limitations prompted the discovery of SST analogues such as octreotide and lanreotide, which have longer half-lives and therefore can be administered as injections. SST analogues are used to treat different forms of HH in children and adults and therapeutic effect is achieved by suppressing insulin secretion from pancreatic β-cells by complex mechanisms. These treatments are associated with several side effects, especially in the newborn period, with necrotizing enterocolitis being the most serious side effect and hence SS analogues should be used with extreme caution in this age group.

scholarly journals Effect of Saurauia vulcani Korth. Leaves on Superoxide Dismutase, HbA1c Levels and Insulin Expression in Hyperglycemic Rats

2019 ◽  
Vol 7 (22) ◽  
pp. 3741-3744
Author(s):  
Chemayanti Surbakti ◽  
Panal Sitorus ◽  
Rosidah Rosidah ◽  
Denny Satria
Keyword(s):  
Superoxide Dismutase ◽  
Blood Glucose ◽  
Ethanol Extract ◽  
Glycosylated Haemoglobin ◽  
Diabetic Rat ◽  
Β Cells ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Insulin Expression ◽  
Hba1c Levels

AIM: This study aimed to investigate Saurauia vulcani Korth. leaves. the activity of ethanol extract in hypoglycemic, superoxide dismutase (SOD), glycosylated haemoglobin (HbA1c) and detection of insulin expression by immunochemistry METHODS: Saurauia vulcani Korth. Leaves powder was extracted by maceration method with ethanol 96%. The extract was administrated orally in doses of 100 mg/kg BW for 27 days. Diabetes was induced in rats by administered of Nicotinamide (NA) 230 mg/kg BW and streptozotocin (STZ) 65 mg/kg BW. Level of blood glucose, SOD, HbA1c were measured, and histopathology pancreas was observed to determine insulin expression with immunochemistry RESULTS: Ethanol extract of Saurauia vulcani Korth. Leaves (EESL) shown a significantly (*p < 0.05) reduced in blood glucose levels at 104.25 ± 2.562 mg/dL and HbA1c level at 32.53 ± 0.188 ng/mL, but increased SOD level at 60.64 ± 0.740 pg/mL and histopathology study shown secretion insulin as seen number of expressions of insulin / slice 31.00 ± 0.315. CONCLUSION: The result of this study showed EESL possess the hypoglycemic activity and increase the level of SOD but decrease the level of HbA1c in diabetic rat condition. The mechanism of the activity is suggested by stimulating the insulin secretion of pancreas β-cells which were damaged.

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