scholarly journals Relationship between some variables of protein profile and indicators of lipomobilization in dairy cows after calving

2014 ◽  
Vol 57 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Csilla Tóthová ◽  
Oskar Nagy ◽  
Gabriel Kováč
Keyword(s):  
Dairy Cows ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Protein Profile ◽  
Total Proteins ◽  
Amyloid A ◽  
Esterified Fatty Acids ◽  
Positive Correlations ◽  
Altered Lipid Metabolism ◽  
Altered Lipid

Abstract. The objective of this study was to determine the concentrations of the main indicators of lipomobilization and selected variables of protein profile in dairy cows after calving, including immunoglobulins and acute phase proteins, as well as to evaluate the relationships between the altered lipid metabolism and changes in protein profile. Into the evaluation we included 54 clinically healthy dairy cows of a Slovak spotted breed, low-land black spotted breed and their crossbreeds in the period of 1-2 weeks after parturition. Blood samples were analysed for non-esterified fatty acids (NEFA, mmol/l), β-hydroxybutyrate (BHB, mmol/l), total proteins (TP, g/l), albumin (Alb, g/l), immunoglobulin G (IgG, g/l), haptoglobin (Hp, g/l) and serum amyloid A (SAA, mg/l). In cows with concentrations of NEFA above 0.35 mmol/l (n=20) we found significantly lower mean serum concentrations of total proteins, albumin and IgG than in cows with serum NEFA concentrations below 0.35 mmol/l (n=34) (P<0.001). On the other hand, cows with higher values of NEFA showed significantly higher mean concentrations of BHB, Hp and SAA (P<0.001). The concentrations of NEFA significantly negatively correlated with the values of TP (P<0.001), albumin (P<0.01) and IgG (P<0.001). Significant positive correlations were found between the concentrations of NEFA and BHB, Hp, as well as SAA (P<0.001). Similar correlations were also found between the values of BHB and the variables of protein profile except for albumin. This study indicates strong relationships between NEFA and selected variables of protein profile in cows after parturition.

scholarly journals Acute Phase Proteins and Variables of Protein Metabolism in Dairy Cows during the Pre- and Postpartal Period

2008 ◽  
Vol 77 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Cs. Tóthová ◽  
O. Nagy ◽  
H. Seidel ◽  
J. Konvičná ◽  
Z. Farkašová ◽  
...  
Keyword(s):  
Blood Serum ◽  
Dairy Cows ◽  
Acute Phase ◽  
Protein Metabolism ◽  
Metabolic Disorders ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Total Proteins ◽  
Mean Values ◽  
Amyloid A

The objective of the present study was to compare the concentrations of acute phase proteins and selected variables of protein metabolism in dairy cows of the Slovak Spotted breed from 4 weeks before parturition to 10 weeks after parturition. Acute phase proteins - haptoglobin (Hp) and serum amyloid A (SAA) - and variables of protein metabolism - total proteins, albumin, urea, creatinine, total immunoglobulins - were evaluated in blood serum. Significant differences were found in average values of the Hp and SAA concentrations in several groups during the monitored period (P < 0.05 and P < 0.001, respectively). The Hp and SAA concentrations in cows during the early postparturient period were significantly higher compared with the later postparturient period. Throughout the monitored time we found differences in mean values of total proteins (P < 0.001), urea (P < 0.001), and total immunoglobulins (P < 0.05). The concentrations of albumin decreased progressively in the postparturient period until 4 weeks after parturition. In the assessment of correlations between the monitored variables in the mentioned period we found significant correlations between Hp and SAA (r = 0.916; P < 0.001), total proteins and urea (r = 0.668; P < 0.05), total proteins and total immunoglobulins (r = 0.827; P < 0.01), and between total immunoglobulins and urea (r = 0.899; P < 0.001). The above mentioned results indicate that in the time around parturition there are significant changes in concentrations of acute phase proteins, as well as in the whole protein metabolism of dairy cows. These facts suggest that the postparturient period is a critical biological phase, throughout which there is the highest incidence of metabolic disorders.

scholarly journals Acute Phase Proteins and their Relation to Energy Metabolites in Dairy Cows during the Pre- and Postpartal Period

2009 ◽  
Vol 78 (3) ◽  
pp. 441-447 ◽  
Author(s):  
Gabriel Kováč ◽  
Csilla Tóthová ◽  
Oskar Nagy ◽  
Herbert Seidel ◽  
Jana Konvičná
Keyword(s):  
Fatty Acids ◽  
Energy Metabolism ◽  
Dairy Cows ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Reproduction Cycle ◽  
Amyloid A ◽  
Esterified Fatty Acids

The objective of this study was to evaluate the concentrations of selected acute phase proteins – haptoglobin (Hp) and serum amyloid A (SAA), and selected variables of energy metabolism in blood serum of dairy cows during different stages of the pre- and postpartal period. The analyses were performed in dairy cows of the Slovak spotted breed and its crossbreeds (n = 57). The cows were divided into 9 groups according to the evaluated part of reproduction cycle – from 4 weeks before parturition to 10 weeks after parturition. Significant differences were found in mean Hp and SAA concentrations during the monitored period (P < 0.05 and P < 0.001, respectively). Cows during the early postparturient period had significantly higher Hp and SAA concentrations compared to cows in later postparturient period (P < 0.05). Significant differences throughout the period under study was found also in means of total cholesterol (P < 0.01), triglycerides (P < 0.001), and non-esterified fatty acids (P < 0.001). Concentrations of Hp during this period were positively correlated with SAA (R = 0.919; P < 0.001). Moreover, significant correlations were found between Hp and non-esterified fatty acids, Hp and β-hydroxybutyrate, as well as between SAA and non-esterified fatty acids. Our results indicate that the acute phase response occurs in cows around parturition, and suggest that there are relationships between mediators of immune response and several indices of energy metabolism. Our results also suggest that in cows with higher concentrations of non-esterified fatty acids, indicating a certain degree of lipid mobilisation, higher values of acute phase proteins may be found.

scholarly journals Correlations between acute phase proteins and the body condition score

2017 ◽  
Vol 86 (4) ◽  
pp. 339-344
Author(s):  
Mária Vargová ◽  
Rudolf Hromada ◽  
Katarína Veszelits Laktičová ◽  
Terézia Pošiváková ◽  
Juraj Benculák ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Acute Phase ◽  
Body Condition ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Body Condition Score ◽  
The Body ◽  
Serum Amyloid ◽  
Amyloid A ◽  
Condition Score

The objective of this study was to evaluate the dynamics of the body condition score, acute phase proteins (serum amyloid A and haptoglobin) and their relationships in dairy cows of the Slovak Pied Cattle from 3 weeks before parturition to 9 weeks after parturition. Significant differences were found in the means of serum amyloid A (P < 0.01). Haptoglobin concentrations increased from 172.0 ± 0.05 µg/ml to 173.0 ± 0.13 µg/ ml at week 3 and week 1 before the parturition, respectively. The highest value of haptoglobin was recorded in cows 3 week post partum. Cows during the early postparturient period had significantly higher serum amyloid A concentrations compared to cows in the prepartal period (P < 0.01). Significantly higher body condition score values were observed in dry cows (from 4.42 ± 0.20 to 4.25 ± 0.15 points) than in cows after parturition (from 3.90 ± 0.12 to 3.25 ± 0.08 points) (P < 0.001). In the assessment of correlations between the selected acute phase proteins significant positive correlation was found (r = 0.213, P < 0.05). Moreover, negative correlations were found between the body condition score and acute phase proteins. Our results show a strong relationship between the decrease of the body condition score and the increase of acute phase proteins in dairy cows around parturition which indicates the process of a certain form of non-specific inflammation at loss of body fat.

scholarly journals Determination of selected acute phase proteins during the treatment of limb diseases in dairy cows

2008 ◽  
Vol 53 (No. 4) ◽  
pp. 173-183 ◽  
Author(s):  
P. Jawor ◽  
S. Steiner ◽  
T. Stefaniak ◽  
W. Baumgartner ◽  
A. Rzasa
Keyword(s):  
Treatment Group ◽  
Dairy Cows ◽  
Acute Phase ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Acute Phase Protein ◽  
Serum Amyloid ◽  
Blood Samples ◽  
Amyloid A ◽  
Phase Protein

The purpose of this study was to assess the diagnostic value of fibrinogen, haptoglobin, and serum amyloid A determination in the monitoring of the treatment of limb diseases in dairy cows. Fourteen lame cows were examined, while 10 clinically healthy cows constituted the control group. Blood samples from the ill animals were collected on three occasions: (1) upon arrival at the clinic, (2) between the third and sixth day after arriving, and (3) upon return to the owner. Blood samples from the control cows were collected once. Plasma levels of fibrinogen, haptoglobin, serum amyloid A, and total serum protein and its fractions (albumin, &alpha;-, &beta;-, &gamma;-globulins) were measured. Significantly higher fibrinogen, haptoglobin, and serum amyloid A levels were observed in the affected cows upon arrival at the clinic than in the control cows. Based on the changes in fibrinogen, haptoglobin, and serum amyloid A concentrations, the cows were divided into those with a systematic decrease in acute-phase protein levels during treatment (Group I, <I>n</I> = 6) and those which showed an increase in one or more acute-phase proteins despite treatment (Group II, <I>n</I> = 8). A stepwise decrease in the examined acute-phase proteins was observed in the first group and indicated an uncomplicated course of treatment; however, treatment of the second group did not appear to be wholly successful. A majority of the cows under treatment (<I>n</I> = 13) exhibited abnormal levels of the examined acute-phase proteins upon return to the owner. This indicates that these patients did not recover completely. The monitoring of plasma acute-phase protein concentrations can be a valuable complement to the clinical assessment of the treatment course and in the early detection of disease complications.

scholarly journals Interrelationship between Somatic Cell Count and Acute Phase Proteins in Serum and Milk of Dairy Cows

2007 ◽  
Vol 76 (1) ◽  
pp. 51-57 ◽  
Author(s):  
G. Kováč ◽  
M. Popelková ◽  
Ľ. Tkáčiková ◽  
O. Burdová ◽  
O. Ihnát
Keyword(s):  
Dairy Cows ◽  
Somatic Cell ◽  
Cell Count ◽  
Acute Phase ◽  
Somatic Cell Count ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Serum Amyloid ◽  
Amyloid A ◽  
Milk Samples

The aim of the present study was to compare the concentrations of two acute phase proteins, haptoglobin (Hp) and serum amyloid A (SAA), in serum and milk of Holstein-Friesian dairy cows grouped according to somatic cell count thresholds (< 100,000 < 400,000 < 400,000 cells/ml) in composite milk samples. The SCC was assessed quantitatively by FOSSOMATIC 90 analyser; serum and milk Hp and SAA concentrations were determined using commercial ELISA kits (Tridelta Development, Ltd., Wicklow, Ireland). We have found a significantly higher Hp and SAA concentrations in the group with SCC > 400,000 cells/ml compared to groups with lower SCC thresholds in composite milk samples. In contrast, significant differences were not demonstrated between the groups in Hp and SAA concentrations determined in serum. The concentrations of Hp in serum and milk were found to be correlated (r = 0.69, P = 0.0003), whereas there was a lower correlation trend in the case of serum versus milk concentrations of SAA (r = 0.43, P = 0.0478). High significant correlations were observed either between milk haptoglobin values and SCC or milk serum amyloid A concentrations and SCC (r = 0.83, r = 0.81, P < 0.0001; respectively). An increase in SCC in cows suffering from mastitis has been accompanied by strong elevation of the milk Hp and SAA, significantly correlated with serum Hp (r = 0.64, P = 0.0014) and poorly correlated with SAA values determined in serum (r = 0.43, P = 0.0478). According to the results obtained in this study we can conclude that measurements of the acute phase proteins, haptoglobin and serum amyloid A predominantly in milk, may be a useful tool in diagnosing mastitis and may be a useful marker of milk quality.

The Effect of Sedation, Oral Examination, and Odontoplasty on Systemic Inflammation as Measured by Serum Amyloid A in the Adult Performance Horse

2019 ◽  
Vol 36 (3) ◽  
pp. 198-201
Author(s):  
Sheri S. W. Birmingham ◽  
Rocky M. Mason
Keyword(s):  
Systemic Inflammation ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Systemic Disease ◽  
Oral Examination ◽  
Serum Amyloid ◽  
Amyloid A ◽  
Adult Performance ◽  
Normal Limits ◽  
Time Periods

Serum amyloid A (SAA) is one of the major acute phase proteins in horses. It serves as a marker for systemic inflammation and infection, as the concentration can increase 100- to even 1000-fold during systemic disease processes. The objective of this study was to evaluate the effect of sedation, oral examination, and odontoplasty on systemic inflammation as measured by SAA in the adult performance horse. This study included 32 clinically healthy adult performance horses. Blood samples were collected immediately prior to sedation, oral examination, and odontoplasty and 48 and 72 hours afterward. Serum amyloid A levels were measured directly after venipuncture using a commercially available stall-side lateral flow immunoassay test developed and validated for equine SAA levels. Serum amyloid A values were within normal limits for each of the time periods and there were no significant differences in SAA values between the time periods. The results of this study suggest that sedation, oral examination, and odontoplasty have no systemic inflammatory effects as measured by SAA.

scholarly journals Association between Anti-Müllerian Hormone Concentration and Inflammation Markers in Serum during the Peripartum Period in Dairy Cows

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1241
Author(s):  
Hiroaki Okawa ◽  
Danielle Monniaux ◽  
Chihiro Mizokami ◽  
Atsushi Fujikura ◽  
Toshihiro Takano ◽  
...  
Keyword(s):  
Vaginal Discharge ◽  
Serum Amyloid A ◽  
Hormone Concentration ◽  
Inflammation Markers ◽  
Somatic Cell Counts ◽  
Amyloid A ◽  
Cell Counts ◽  
Esterified Fatty Acids ◽  
Significant Difference ◽  
Peripartum Period

The relationships between changes in anti-Müllerian hormone (AMH) concentration and various traits, including milk somatic cell counts (SCC), were evaluated. Blood samples were collected from 43 Holstein cows 14 days before (D-14) and 10 (D10) and 28 days after (D28) parturition, and vaginal discharge score (VDS) and polymorphonuclear leukocyte (PMNL) percentages were assessed in endometrial samples at D28. Cows were separated into four quartiles (Q1–Q4) based on changes in AMH concentration during the peripartum period (AMH ratio: D28/D-14). Correlations between AMH ratio and each parameter were evaluated and classified into high-AMH (Q4, 1.83 ± 0.12, n = 11) and low-AMH (Q1, 0.83 ± 0.05, n = 11) groups. The AMH ratio was positively correlated with magnesium and non-esterified fatty acids levels, and the albumin/globulin ratio at D10 and D28, but negatively correlated with serum amyloid A (SAA) at D10. SAA and γ-globulin levels were significantly higher in the low-AMH group at D28. There was no significant difference in VDS, PMNL percentage, and milk SCC between the two groups. The decreasing AMH ratio from the prepartum to the postpartum period corresponds to high inflammation biomarker levels. Whether it subsequently affects the reproductive prognosis of postpartum cows needs further investigations.

Acute Phase Proteins (Haptoglobin and Serum Amyloid A) and Pro-Inflammatory Cytokines (IL-12 and IL-10) in Cows Vaccinated with Prototype Killed S. aureus Mastitis Vaccine and Challenged with S. aureus Mastitis Infection.

2020 ◽  
Author(s):  
Idris Umar Hambali ◽  
Faez Firdaus Jesse Bin Abdallah ◽  
Khaleequl rahaman Bhuttu ◽  
Azmi M Lila ◽  
zunita Zakaria ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Serum Amyloid ◽  
Bacterial Challenge ◽  
Amyloid A ◽  
Significant Difference ◽  
Group B ◽  
Pro Inflammatory Cytokines ◽  
Friesian Cows

Abstract Background The economic downturn experienced by farmers and the fear of milk borne infection are of a greater public health concern. Haptoglobin, Serum Amyloid A, IL-12 and IL-10 in lactating Friesian cows vaccinated with prototype killed S. aureus mastitis vaccine and challenged with S. aureus were evaluated. Bacterin concentration at 10 8 cfu /ml of the local isolate of S. aureus was adjuvanted with KAl(SO₄)₂. Six lactating Friesian cows were grouped into A= Negative control, B = Positive control and C = vaccine group. Group C was vaccinated intramuscularly with 2ml of the monovalent vaccine, groups A and B with physiologic normal saline. Groups B and C were later challenged with the live bacterium via intramammary route . Result There was a significant increase in IL-10 concentration in vaccinated group post primary vaccination (PPV), booster phase (PB) and during the bacterial challenge phase. There was also a significantly increased IL-12 concentration in the vaccinated group at 24 hours, weeks 1 and 2 PPV. Haptoglobin at 12 and 24 hours PPV had a significant difference in group C. During the PB at 8 and 12 hours there was a significant difference in group C. During the bacterial challenged phase at 0, 3, 24 hours and day 7 PC there was a significant difference in group B. At 8 hours PC there was a significant difference in group C. For Serum Amyloid A, during PPV at 0, 3, 8, 12, 24 hours and weeks 1 and 2, the concentrations was significantly different in groups C. During PB at 0, 3, 8 and 12 hours PB there was a significant difference in groups C. During the bacterial challenge phase at 3, 8, 12, 24 hours, days 7 and 14 PC there was a significant difference in group B. At 0 hour PC there was a significant increase observed in group C. Conclusion The developed prototype killed S. aureus mastitis vaccine using local isolates was able to stimulate acute phase proteins and pro-inflammatory cytokines. The pattern of responses PC indicated protection, thereby suggesting that vaccination can protect against mastitis infection in dairy cows.

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