scholarly journals Robust, coherent, and synchronized circadian clock-controlled oscillations along Anabaena filaments

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Rinat Arbel-Goren ◽  
Valentina Buonfiglio ◽  
Francesca Di Patti ◽  
Sergio Camargo ◽  
Anna Zhitnitsky ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Spatial Coherence ◽  
Cell Communication ◽  
High Amplitude ◽  
Circadian Clocks ◽  
Filamentous Cyanobacterium ◽  
Oscillatory Circuit ◽  
Genes Encoding ◽  
Spatio Temporal ◽  
The Individual

Circadian clocks display remarkable reliability despite significant stochasticity in biomolecular reactions. We study the dynamics of a circadian clock-controlled gene at the individual cell level in Anabaena sp. PCC 7120, a multicellular filamentous cyanobacterium. We found significant synchronization and spatial coherence along filaments, clock coupling due to cell-cell communication, and gating of the cell cycle. Furthermore, we observed low-amplitude circadian oscillatory transcription of kai genes encoding the post-transcriptional core oscillatory circuit and high-amplitude oscillations of rpaA coding for the master regulator transducing the core clock output. Transcriptional oscillations of rpaA suggest an additional level of regulation. A stochastic one-dimensional toy model of coupled clock cores and their phosphorylation states shows that demographic noise can seed stochastic oscillations outside the region where deterministic limit cycles with circadian periods occur. The model reproduces the observed spatio-temporal coherence along filaments and provides a robust description of coupled circadian clocks in a multicellular organism.

scholarly journals Selected In Situ Hybridization Methods: Principles and Application

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3874
Author(s):  
Dominika Veselinyová ◽  
Jana Mašlanková ◽  
Katarina Kalinová ◽  
Helena Mičková ◽  
Mária Mareková ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Imaging Techniques ◽  
Cell Communication ◽  
Probe Design ◽  
Rapid Progress ◽  
In Situ Techniques ◽  
Different Types ◽  
Laboratory Procedures ◽  
The Individual

We are experiencing rapid progress in all types of imaging techniques used in the detection of various numbers and types of mutation. In situ hybridization (ISH) is the primary technique for the discovery of mutation agents, which are presented in a variety of cells. The ability of DNA to complementary bind is one of the main principles in every method used in ISH. From the first use of in situ techniques, scientists paid attention to the improvement of the probe design and detection, to enhance the fluorescent signal intensity and inhibition of cross-hybrid presence. This article discusses the individual types and modifications, and is focused on explaining the principles and limitations of ISH division on different types of probes. The article describes a design of probes for individual types of in situ hybridization (ISH), as well as the gradual combination of several laboratory procedures to achieve the highest possible sensitivity and to prevent undesirable events accompanying hybridization. The article also informs about applications of the methodology, in practice and in research, to detect cell to cell communication and principles of gene silencing, process of oncogenesis, and many other unknown processes taking place in organisms at the DNA/RNA level.

scholarly journals Holocene changes in African vegetation: tradeoff between climate and water availability

2014 ◽  
Vol 10 (2) ◽  
pp. 681-686 ◽  
Author(s):  
C. Hély ◽  
A.-M. Lézine ◽  
APD contributors
Keyword(s):  
West Africa ◽  
Water Availability ◽  
Regional Scale ◽  
Plant Distribution ◽  
Climate Conditions ◽  
Tropical Plants ◽  
Plant Migration ◽  
Spatio Temporal ◽  
The Individual ◽  
Instrumental Records

Abstract. Although past climate change is well documented in West Africa through instrumental records, modeling activities, and paleo-data, little is known about regional-scale ecosystem vulnerability and long-term impacts of climate on plant distribution and biodiversity. Here we use paleohydrological and paleobotanical data to discuss the relation between available surface water, monsoon rainfall and vegetation distribution in West Africa during the Holocene. The individual patterns of plant migration or community shifts in latitude are explained by differences among tolerance limits of species to rainfall amount and seasonality. Using the probability density function methodology, we show here that the widespread development of lakes, wetlands and rivers at the time of the "Green Sahara" played an additional role in forming a network of topographically defined water availability, allowing for tropical plants to migrate north from 15 to 24° N (reached ca. 9 cal ka BP). The analysis of the spatio–temporal changes in biodiversity, through both pollen occurrence and richness, shows that the core of the tropical rainbelt associated with the Intertropical Convergence Zone was centered at 15–20° N during the early Holocene wet period, with comparatively drier/more seasonal climate conditions south of 15° N.

Circadian clock precision, health, and longevity

2021 ◽  
Vol 23 (1) ◽  
pp. 3-15
Author(s):  
D. G. Gubin ◽  
◽  
S. N. Kolomeichuk ◽  
D. Weinert ◽  
◽  
...  
Keyword(s):  
Health Status ◽  
Circadian Rhythms ◽  
Life Expectancy ◽  
Circadian Clock ◽  
Animal Species ◽  
Genetic Factors ◽  
High Amplitude ◽  
Phenotypic Trait ◽  
Precision Health ◽  
Lower Morbidity

An accurate circadian clock, associated with the precise intrinsic period, tau, can be linked with a definite chronotype and health status. Exemplified by different animal species, and, as we argue herein, may as well be in humans, endogenous tau close enough to 24 hours is associated with higher life expectancy, lower morbidity, and is possibly adhered to moderate morning chronotype. Accurate circadian tau facilitates maintaining a high amplitude of circadian rhythms, a phenotypic trait that is related to health and longevity. Some genetic factors that coordinate tau and ensure circadian clock precision is considered.

THE SPACES OF MYTH IN KAZUO ISHIGURO’S THE REMAINS OF THE DAY: EXPLORING THE INTERSECTIONS OF MEMORY AND HISTORY

Ensemble ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 234-242
Author(s):  
Medha Bhadra Chowdhury ◽  
Keyword(s):  
Identity Politics ◽  
Cultural Beliefs ◽  
Traditional Values ◽  
Confined Space ◽  
Spatial Dimensions ◽  
Temporal Expressions ◽  
Spatio Temporal ◽  
Post War ◽  
The Individual ◽  
Historical Past

Kazuo Ishiguro’s The Remains of the Day (1989) reconstructs the experiences of an ageing butler, Stevens, trapped within the confined space of the house he has served in for many years. The contours of memory are drawn along the spatial dimensions of the house which serve as a space of contestation between traditional values and emergent cultural beliefs in the post-war period. Physical modifications on the architecture produce continuities and alterations within the subject, who inhabits the space. This paper seeks to explore the dynamics of remembering and forgetting which are determined by the sites of memory and which trace historical changes as well as shifts in identity politics in Ishiguro’s novel. The paper critically assesses the idea of space, its functional dimension and mythic commemoration in relation to a symbolic historical past. It examines the development of subjectivity through the expansion of memory embodied in material form and the complex relationship between history and myth-making, which complicates individual identity. This paper further proposes that these spatio-temporal expressions can be understood as not only confined to the individual but may be extended to the domain of public memory and contextualized in a post-war British cultural politics of grief.

Spatio-Temporal Gaussianization Flows for Extreme Event Detection

2021 ◽  
Author(s):  
Miguel-Ángel Fernández-Torres ◽  
J. Emmanuel Johnson ◽  
María Piles ◽  
Gustau Camps-Valls
Keyword(s):  
Event Detection ◽  
Land Surface ◽  
Extreme Event ◽  
Learning Approaches ◽  
Proposed Model ◽  
Individual Contributions ◽  
Current Context ◽  
Spatio Temporal ◽  
The Individual ◽  
Anomaly Score

<p>Automatic anticipation and detection of extreme events constitute a major challenge in the current context of climate change. Machine learning approaches have excelled in detection of extremes and anomalies in Earth data cubes recently, but are typically both computationally costly and supervised, which hamper their wide adoption. We alternatively present here an unsupervised, efficient, generative approach for extreme event detection, whose performance is illustrated for drought detection in Europe during the severe Russian heat wave in 2010. The core architecture of the model is generic and could naturally be extended to the detection of other kinds of anomalies. First, it computes hierarchical appearance (spatial) and motion (temporal) representations of several informative Essential Climate Variables (ECVs), including soil moisture, land surface temperature, as well as features describing vegetation health. Then, these representations are combined using Gaussianization Flows that yield a spatio-temporal anomaly score. This allows the proposed model not only to detect droughts areas, but also to explain why they were produced, monitoring the individual contributions of each of the ECVs to the indicator at its output.</p>

Synchronizing the Neurospora crassa circadian clock with the rhythmic environment

2005 ◽  
Vol 33 (5) ◽  
pp. 949-952 ◽  
Author(s):  
N. Price-Lloyd ◽  
M. Elvin ◽  
C. Heintzen
Keyword(s):  
Light Intensity ◽  
Circadian Clock ◽  
Neurospora Crassa ◽  
Model Organism ◽  
Circadian Clocks ◽  
Current Understanding ◽  
Signalling Pathways ◽  
Selection Pressures ◽  
The Earth ◽  
Natural Cycles

The metronomic predictability of the environment has elicited strong selection pressures for the evolution of endogenous circadian clocks. Circadian clocks drive molecular and behavioural rhythms that approximate the 24 h periodicity of our environment. Found almost ubiquitously among phyla, circadian clocks allow preadaptation to rhythms concomitant with the natural cycles of the Earth. Cycles in light intensity and temperature for example act as important cues that couple circadian clocks to the environment via a process called entrainment. This review summarizes our current understanding of the general and molecular principles of entrainment in the model organism Neurospora crassa, a simple eukaryote that has one of the best-studied circadian systems and light-signalling pathways.

scholarly journals Interaction between photoperiod and variation in circadian rhythms in tomato

2022 ◽  
Author(s):  
Yanli Xiang ◽  
Thomas Sapir ◽  
Pauline Rouillard ◽  
Marina Ferrand ◽  
Jose M Jimenez-Gomez
Keyword(s):  
Seasonal Variation ◽  
Circadian Rhythms ◽  
Phase Shift ◽  
Circadian Clock ◽  
Environmental Changes ◽  
Circadian Clocks ◽  
Biological Processes ◽  
Near Isogenic Lines ◽  
Transcriptomic Profiling ◽  
Isogenic Lines

Many biological processes follow circadian rhythmicity and are controlled by the circadian clock. Predictable environmental changes such as seasonal variation in photoperiod can modulate circadian rhythms, allowing organisms to adjust to the time of the year. Modification of circadian clocks is especially relevant in crops to enhance their cultivability in specific regions by changing their sensibility to photoperiod. In tomato, the appearance of mutations in EMPFINDLICHER IM DUNKELROTEN LICHT 1 (EID1, Solyc09g075080) and NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED GENE 2 (LNK2, Solyc01g068560) during domestication delayed its circadian rhythms, and allowed its expansion outside its equatorial origin. Here we study how variation in circadian rhythms in tomato affects its perception of photoperiod. To do this, we create near isogenic lines carrying combinations of wild alleles of EID1 and LNK2 and perform transcriptomic profiling under two different photoperiods. We observe that EID1, but not LNK2, has a large effect on the tomato transcriptome and its response to photoperiod. This large effect of EID1 is likely a consequence of the global phase shift elicited by this gene in tomato's circadian rhythms.

scholarly journals Peripheral Sensory Organs Contribute to Temperature Synchronization of the Circadian Clock in Drosophila melanogaster

2021 ◽  
Vol 12 ◽  
Author(s):  
Rebekah George ◽  
Ralf Stanewsky
Keyword(s):  
Gene Expression ◽  
Drosophila Melanogaster ◽  
Circadian Clock ◽  
Temperature Fluctuations ◽  
Fruit Fly ◽  
Circadian Clocks ◽  
Sensory Cells ◽  
Clock Gene Expression ◽  
External Time ◽  
Peripheral Sensory

Circadian clocks are cell-autonomous endogenous oscillators, generated and maintained by self-sustained 24-h rhythms of clock gene expression. In the fruit fly Drosophila melanogaster, these daily rhythms of gene expression regulate the activity of approximately 150 clock neurons in the fly brain, which are responsible for driving the daily rest/activity cycles of these insects. Despite their endogenous character, circadian clocks communicate with the environment in order to synchronize their self-sustained molecular oscillations and neuronal activity rhythms (internal time) with the daily changes of light and temperature dictated by the Earth’s rotation around its axis (external time). Light and temperature changes are reliable time cues (Zeitgeber) used by many organisms to synchronize their circadian clock to the external time. In Drosophila, both light and temperature fluctuations robustly synchronize the circadian clock in the absence of the other Zeitgeber. The complex mechanisms for synchronization to the daily light–dark cycles are understood with impressive detail. In contrast, our knowledge about how the daily temperature fluctuations synchronize the fly clock is rather limited. Whereas light synchronization relies on peripheral and clock-cell autonomous photoreceptors, temperature input to the clock appears to rely mainly on sensory cells located in the peripheral nervous system of the fly. Recent studies suggest that sensory structures located in body and head appendages are able to detect temperature fluctuations and to signal this information to the brain clock. This review will summarize these studies and their implications about the mechanisms underlying temperature synchronization.

scholarly journals Mediatization, Spatial Coherence and Social Sustainability: The Role of Digital Media Networks in a Swedish Countryside Community

2010 ◽  
Vol 2 (2) ◽  
pp. 177-192 ◽  
Author(s):  
André Jansson
Keyword(s):  
Rural Communities ◽  
Digital Media ◽  
Communication Networks ◽  
Local Community ◽  
Spatial Coherence ◽  
Social Sustainability ◽  
Ethnographic Study ◽  
Individual Level ◽  
Communicative Patterns ◽  
The Individual

What does the implementation of new communication networks mean for the spatial coherence and social sustainability of rural communities? This paper takes its key from Wittel’s discussion of network sociality, understood as the opposite of Gemeinschaft. Wittel’s argument may inform our understanding of how communicative patterns in rural communities are partly reembedded through ongoing media transitions. But it must also be problematized. Relating Wittel’s discussion to Halfacree’s model of spatial coherence and Urry’s notion of network capital, as well as to findings from an ethnographic study in a Swedish countryside community, a more complex view is presented. It is argued that global communication networks under rural conditions contribute to the integration and sustainability of the community, as much as to processes of expansion and differentiation. The results show that network sociality and community constitute interdependent concepts. Through their capacity of linking people to external realms of interest, while simultaneously reinforcing their sense of belonging in the local community, online media promote ontological security at the individual level, thus operating as a social stabilizer.

Sign in / Sign up

Export Citation Format

Share Document