scholarly journals Therapeutic effects of astragaloside IV and Astragalus spinosus saponins against bisphenol A-induced neurotoxicity and DNA damage in rats

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11930
Author(s):  
Amina E. Essawy ◽  
Heba-Tallah Abd Elrahim Abd Elkader ◽  
Omaima A. Khamiss ◽  
Saber Mohamed Eweda ◽  
Heba Mohamed Abdou
Keyword(s):  
Dna Damage ◽  
Bisphenol A ◽  
Brain Regions ◽  
Male Rats ◽  
Therapeutic Effects ◽  
Sprague Dawley ◽  
Astragaloside Iv ◽  
Adult Age ◽  
Ameliorative Effect ◽  
Weaning Age

Background Bisphenol A (BPA) is an endocrine disruptor to which humans are often subjected during daily life. This study aimed to investigate the ameliorative effect of astragaloside IV (ASIV) or saponins extracted from Astragalus spinosus (A. spinosus) against DNA damage and neurotoxic effects induced by BPA in prefrontal cortex (PFC), hippocampal and striatal brain regions of developing male rats. Materials and Methods Juvenile PND20 (pre-weaning; age of 20 days) male Sprague Dawley rats were randomly and equally divided into four groups: control, BPA, BPA+ASIV and BPA+A. spinosus saponins groups. Bisphenol A (125 mg/kg/day) was administrated orally to male rats from day 20 (BPA group) and along with ASIV (80 mg/kg/day) (BPA+ASIV group) or A. spinosus saponin (100 mg/kg/day) (BPA+ A. spinosus saponins group) from day 50 to adult age day 117. Results Increased level of nitric oxide (NO) and decreased level of glutamate (Glu), glutamine (Gln), glutaminase (GA) and glutamine synthetase (GS) were observed in the brain regions of BPA treated rats compared with the control. On the other hand, co-administration of ASIV or A. spinosus saponin with BPA considerably improved levels of these neurochemicals. The current study also revealed restoration of the level of brain derived neurotrophic factor (BDNF) and N-methyl-D-aspartate receptors (NR2A and NR2B) gene expression in BPA+ ASIV and BPA+A. spinosus saponins groups. The co-treatment of BPA group with ASIV or A. spinosus saponin significantly reduced the values of comet parameters as well as the intensity of estrogen receptors (ERs) immunoreactive cells and improved the histological alterations induced by BPA in different brain regions. Conclusion It could be concluded that ASIV or A. spinosus saponins has a promising role in modulating the neurotoxicity and DNA damage elicited by BPA.

Refining the Effects Observed in a Developmental Neurobehavioral Study of Ammonium Perchlorate Administered Orally in Drinking Water to Rats. II. Behavioral and Neurodevelopment Effects

2005 ◽  
Vol 24 (6) ◽  
pp. 451-467 ◽  
Author(s):  
Raymond G. York ◽  
John Barnett ◽  
Michael F. Girard ◽  
David R. Mattie ◽  
Marni V. K. Bekkedal ◽  
...  
Keyword(s):  
Drinking Water ◽  
Ammonium Perchlorate ◽  
Brain Regions ◽  
Male Rats ◽  
Male Rat ◽  
Sprague Dawley ◽  
Continuous Access ◽  
Brain Morphometry ◽  
The Central Nervous System ◽  
The Right

A developmental neurotoxicity study was conducted to generate additional data on the potential functional and morphological hazard to the central nervous system caused by ammonium perchlorate in offspring from in utero and lactation exposure. Female Sprague-Dawley rats (23 to 25/group) were given continuous access to 0 (carrier), 0.1, 1.0, 3.0, and 10.0 mg/kg-day perchlorate in the drinking water beginning 2 weeks prior to mating and continuing through day 10 of lactation for the behavioral function assessment or given continuous access to 0 (carrier), 0.1, 1.0, 3.0, and 30.0 mg/kg-day beginning on gestation day 0 and continuing through day 10 of lactation for neurodevelopment assessments. Motor activity was conducted on postpartum days 14, 18, and 22 and juvenile brain weights, neurohistopathological examinations, and regional brain morphometry were conducted on postpartum days 10 and 22. This research revealed a sexually dimorphic response, with some brain regions being larger in perchlorate-treated male rats than in comparable controls. Even so, there was no evidence of any obvious exposure-related effects on male rat brain weights or neuropathology. The most consistent exposure-related effect in the male pups was on the thickness of the corpus callosum, with both the right- and left-sided measures of the thickness of this white matter tract being significantly greater for the male pups in the 0.1 and 1.0 mg/kg-day exposure groups. The behavioral testing suggests prenatal exposure to ammonium perchlorate does not affect the development of gross motor movements in the pups.

Synthesis, Characterization of Silver Nanoparticles Using Nigella sativa Seeds and Study Their Effects on the Serum Lipid Profile and DNA Damage on the Rats’ Blood Treated with Hydrogen Peroxide

2019 ◽  
Vol 43 (2) ◽  
pp. 23-37
Author(s):  
Zainab Sattar Ali
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Silver Nanoparticles ◽  
Lipid Profile ◽  
Dna Fragmentation ◽  
Nigella Sativa ◽  
Tap Water ◽  
Male Rats ◽  
High Dose ◽  
Ameliorative Effect

This study was aimed to produce silver nanoparticles using aqueous extract of Nigella sativa, also to investigate the effects of green synthesized Nigella sativa seeds silver nanoparticles on dyslipidemia and DNA fragmentation in hydrogen peroxide-exposed rats. The produced Nigella sativa seeds silver nanoparticles were characterized through Ultraviolet-Visible spectroscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction (XRD) style, and Scanning Electron Microscope was used to investigate the morphology and size of synthesized Nigella sativa seeds silver nanoparticles. Forty adults male rats were randomly and equally divided into five groups and daily  had been remedying for two months as followings:  G1 group (Control), G2 group, rats in this group were received tap water containing 1%  H2O2, animals in G3 and G4 groups were injected IP  Nigella sativa seeds silver nanoparticles 25 and 50 mg/kg B.W., respectively, and received ordinary tap water containing 1% H2O2 , and G5 group, animals in this group were injected IP  Nigella sativa seeds extract  50 mg/kg B.W. and received ordinary tap water containing 1% H2O2. Blood samples collected after one and two months of the experiment from each animal for DNA fragmentation measurements and serum estimated lipid profile.The results receded a case of dyslipidemia,  as well  significant elevation in DNA damage in G4 and G2 groups The results also confirmed the hypolipidemic and cytoprotective effect of Nigella sativa seeds extract (G5 group) and silver nanoparticles as25mg/kg B.W (group G3) clarified by correction of dyslipidemia, alongside significant alleviation in DNA damage. In conclusion, the results in the current study effectsof Nigella sativa seeds silver nanoparticles at high dose and documents the ameliorative effect of Nigella sativaseeds extract and Nigella sativa seeds silver nanoparticles on lipid profile and DNA damage.

Therapeutic effects of Glycyrrhiza glabra aqueous extract ointment on cutaneous wound healing in Sprague Dawley male rats

2019 ◽  
Vol 28 (5) ◽  
pp. 1507-1514 ◽  
Author(s):  
Akram Zangeneh ◽  
Mehrdad Pooyanmehr ◽  
Mohammad Mahdi Zangeneh ◽  
Rohallah Moradi ◽  
Raheleh Rasad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Aqueous Extract ◽  
Glycyrrhiza Glabra ◽  
Male Rats ◽  
Therapeutic Effects ◽  
Cutaneous Wound Healing ◽  
Sprague Dawley ◽  
Cutaneous Wound

Melatonin ameliorates bisphenol A-induced DNA damage in the germ cells of adult male rats

2013 ◽  
Vol 752 (1-2) ◽  
pp. 57-67 ◽  
Author(s):  
Hong-Juan Wu ◽  
Chuan Liu ◽  
Wei-Xia Duan ◽  
Shang-Cheng Xu ◽  
Min-Di He ◽  
...  
Keyword(s):  
Dna Damage ◽  
Bisphenol A ◽  
Germ Cells ◽  
Adult Male ◽  
Male Rats

Ameliorative effect of quercetin against arsenic-induced sperm DNA damage and daily sperm production in adult male rats

2015 ◽  
Vol 39 (3) ◽  
pp. 290-296 ◽  
Author(s):  
Sarwat Jahan ◽  
Saima Rehman ◽  
Hizb Ullah ◽  
Asma Munawar ◽  
Qurat Ul Ain ◽  
...  
Keyword(s):  
Dna Damage ◽  
Adult Male ◽  
Male Rats ◽  
Sperm Production ◽  
Sperm Dna Damage ◽  
Sperm Dna ◽  
Ameliorative Effect ◽  
Daily Sperm Production

scholarly journals Therapeutic effects of bone marrow mesenchymal stem cells via modulation of TLR2 and TLR4 on renal ischemia-reperfusion injury in male Sprague-Dawley rats

Bioimpacts ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 219-226
Author(s):  
Zeinab Karimi ◽  
Sahar Janfeshan ◽  
Elias Kargar Abarghouei ◽  
Seyedeh-Sara Hashemi
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Therapeutic Potential ◽  
Ischemia Reperfusion ◽  
Renal Ischemia ◽  
Male Rats ◽  
Great Promise ◽  
Therapeutic Effects ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

Introduction: Acute kidney injury (AKI) induced by renal ischemia-reperfusion (I/R) injury is a pro-inflammatory process that activates toll-like receptors (TLRs). Stem cell therapy holds a great promise for kidney repair. Therefore, we investigated the immunomodulatory role of bone marrow stromal cells (BMSCs) on TLR2 and TLR4 expression in AKI in male Sprague-Dawley rats. Methods: BMSCs were isolated from the bone marrow of male rats, cultured in DMEM, and characterized using appropriate markers before transplantation. Renal I/R was induced by 45 minutes bilateral ischemia followed by 24 hours of reperfusion. Rats received intraperitoneal injections of BMSCs (1.5 × 106 cells, i.p, per rat) immediately after termination of renal ischemia. Serum samples were collected pre-and post-stem cells injection for assessment of blood urea nitrogen (BUN) and creatinine (Cr) levels. The kidneys were harvested after 24 hours of reperfusion for structural and molecular analysis. Results: Renal I/R caused severe tissue injuries and increased the level of BUN (166.5 ± 12.9 vs. 18.25 ± 1.75) and Cr (3.7 ± 0.22 vs. 0.87 ± 0.06) compared to the sham group. In addition, mRNA expression of TLR2 and TLR4 elevated in the renal I/R group. Administration of BMSCs improved the functional and structural state of the kidney induced by I/R and down-regulated TLR2 and TLR4 gene expression. Conclusion: The results showed a highly significant renoprotection by BMSCs that indicates their therapeutic potential in I/R injures. These effects are most likely associated with the TLR2/4 signaling pathway via modulation of the inflammatory response cascades.

scholarly journals Alterations in Serum Myostatin Levels via Swimming Exercise in High Fructose Mediated Metabolic Syndrome Model

2021 ◽  
pp. 40-44
Author(s):  
Muhammed Emre Karaman ◽  
Cengiz Arslan ◽  
Mehmet Ferit Gürsu ◽  
Zelal Arat ◽  
Furkan Türkoğlu
Keyword(s):  
Metabolic Syndrome ◽  
Serum Glucose ◽  
Male Rats ◽  
Swimming Exercise ◽  
Therapeutic Effects ◽  
Sprague Dawley ◽  
Serum Samples ◽  
High Fructose ◽  
Significant Difference ◽  
Metabolic Syndrome Model

Aims: Metabolic syndrome is a table of abdominal diseases characterized by insulin resistance, obesity, dyslipidemia, and hypertension, and it is a serious risk factor for the development of cardiovascular diseases. The purpose of this study was to understand how metabolic syndrome causes a change in myostatin levels, and to understand if swimming exercise have protective and therapeutic effects in fructose mediated metabolic syndrome model by regulating serum myostatin level. Methodology: A total of 21 Sprague Dawley male rats were used in the study. Metabolic syndrome was induced by adding 30% fructose into drinking water for 5 weeks The rats (6-8 weeks old, weighing 200-250 g) were randomly divided into 3 groups as 7 rats in each cage. Swimming exercises were applied three days a week for six weeks. On exercise days, rats were exercised for 20 minutes in a day. Blood was collected from all animals in aprotinin tubes to be used in the necessary analysis with decapitation. Serum samples obtained after centrifugation were kept at -80 ℃ until the assay were performed. In serum samples, myostatin was measured by ELISA method. Results: There was a statistically significant difference resulting from G2, which had the highest value in glucose and triglyceride levels between groups. G2 had the lowest HDL levels. Myostatin levels were significantly higher in G2 compared to other groups. Conclusion: Moderate swimming exercises are protective and therapeutic in preventing chronic diseases such as diabetes, obesity and metabolic syndrome. In line with these results, it can be said that swimming exercise is a therapeutic practice that regulates impaired fasting serum glucose, increased TG levels and decreased HDL levels and downregulates serum myostatin levels.

scholarly journals Therapeutic effects of anthocyanin-rich Hibiscus sabdariffa L. extract on body mass index, lipid profile and fatty liver in obese-hypercholesterolaemic rat model

2019 ◽  
Vol 9 (1) ◽  
pp. 1 ◽  
Author(s):  
Liza Noordin ◽  
Wan Nurul Izzati Wan Mohamad Noor ◽  
Sabreena Safuan ◽  
Wan Amir Nizam Wan Ahmad
Keyword(s):  
Body Mass Index ◽  
Lipid Profile ◽  
Body Mass ◽  
Liver Enzymes ◽  
Normal Diet ◽  
Male Rats ◽  
Therapeutic Effects ◽  
Hibiscus Sabdariffa ◽  
Sprague Dawley ◽  
Group 2

Background: Hibiscus sabdariffa L. or Roselle, is one of the most common flower plants cultivated worldwide. Traditionally, it is claimed to reduce weight and cholesterol level. The aim of this study was to evaluate the effects of anthocyanin-rich Roselle aqueous extract on the physical, biochemical and histological changes in obese-hypercholesterolaemic rat model.Methods: Thirty Sprague-Dawley male rats were divided into five groups (n=6 per group). Group 1 is normal control (NC) rat that was fed with normal diet. The remaining rats (Group 2-5) were fed with commercial high-fat diet (HFD) for 6 weeks to induce obese-hypercholesterolaemic (OH) rat. After induction, the OH rats were divided into 4 groups: OH-Untreated group, OH-ARTE; OH treated with 300 mg/kg Roselle aqueous (anthocyanin-rich) and 1% trifluoroacetic acid extract (ARTE), OH-Orlistat; OH treated with 37 mg/kg orlistat and OH-Atorvastatin; OH treated with 10 mg/kg atorvastatin. After the 3 weeks intervention period, all rats were sacrificed. Body mass index, lipid profile and liver enzymes were evaluated. The liver section was subjected to hematoxylin and eosin staining for histopathological evaluation.Results: This study showed that 3 weeks administration of ARTE significantly reduced BMI, improved levels of lipid profile and liver enzymes, and histopathological changes of liver when compared to OH-untreated rats. The results were also comparable to the standard drugs.Conclusions: This study suggested that Roselle extract constitutes an effective and safe alternate treatment for obesity and hyperlipidaemia.

scholarly journals Three-Generation Study of Male Rats Gestationally Exposed to High Butterfat and Bisphenol A: Impaired Spermatogenesis, Penetrance with Reduced Severity

Nutrients ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 3636
Author(s):  
Shuk-Mei Ho ◽  
Rahul Rao ◽  
Bin Ouyang ◽  
Neville N. C. Tam ◽  
Emma Schoch ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Endocrine Disruptor ◽  
Estrogen Receptor Beta ◽  
Male Rats ◽  
Adult Males ◽  
Sprague Dawley ◽  
Domain 3 ◽  
Data Gap ◽  
F2 Generation ◽  
Generation Offspring

Gestational high butterfat (HFB) and/or endocrine disruptor exposure was previously found to disrupt spermatogenesis in adulthood. This study addresses the data gap in our knowledge regarding transgenerational transmission of the disruptive interaction between a high-fat diet and endocrine disruptor bisphenol A (BPA). F0 generation Sprague-Dawley rats were fed diets containing butterfat (10 kcal%) and high in butterfat (39 kcal%, HFB) with or without BPA (25 µg/kg body weight/day) during mating and pregnancy. Gestationally exposed F1-generation offspring from different litters were mated to produce F2 offspring, and similarly, F2-generation animals produced F3-generation offspring. One group of F3 male offspring was administered either testosterone plus estradiol-17β (T + E2) or sham via capsule implants from postnatal days 70 to 210. Another group was naturally aged to 18 months. Combination diets of HFB + BPA in F0 dams, but not single exposure to either, disrupted spermatogenesis in F3-generation adult males in both the T + E2-implanted group and the naturally aged group. CYP19A1 localization to the acrosome and estrogen receptor beta (ERbeta) localization to the nucleus were associated with impaired spermatogenesis. Finally, expression of methyl-CpG-binding domain-3 (MBD3) was consistently decreased in the HFB and HFB + BPA exposed F1 and F3 testes, suggesting an epigenetic component to this inheritance. However, the severe atrophy within testes present in F1 males was absent in F3 males. In conclusion, the HFB + BPA group demonstrated transgenerational inheritance of the impaired spermatogenesis phenotype, but severity was reduced in the F3 generation.

scholarly journals Developmental Toxicity by Exposure to Bisphenol A Diglycidyl Ether during Gestation and Lactation Period in Sprague-dawley Male Rats

2007 ◽  
Vol 40 (2) ◽  
pp. 155 ◽  
Author(s):  
Un-jun Hyoung ◽  
Yun-jung Yang ◽  
Su-kyoung Kwon ◽  
Jae-hyoung Yoo ◽  
Soon-chul Myoung ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Developmental Toxicity ◽  
Diglycidyl Ether ◽  
Male Rats ◽  
Lactation Period ◽  
Sprague Dawley ◽  
Bisphenol A Diglycidyl Ether
Sign in / Sign up

Export Citation Format

Share Document