scholarly journals Effects of sound exposure on the growth and intracellular macromolecular synthesis ofE. colik-12

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1920 ◽  
Author(s):  
Shaobin Gu ◽  
Yongzhu Zhang ◽  
Ying Wu

Microbes, as one of the primary producers of the biosphere, play an important role in ecosystems. Exploring the mechanism of adaptation and resistance of microbial population to various environmental factors has come into focus in the fields of modern microbial ecology and molecular ecology. However, facing the increasingly serious problem of acoustic pollution, very few efforts have been put forth into studying the relation of single cell organisms and sound field exposure. Herein, we studied the biological effects of sound exposure on the growth ofE. coliK-12 with different acoustic parameters. The effects of sound exposure on the intracellular macromolecular synthesis and cellular morphology ofE. coliK-12 were also analyzed and discussed. Experimental results indicated thatE. coliK-12 exposed to sound waves owned a higher biomass and a faster specific growth rate compared to the control group. Also, the average length ofE. coliK-12 cells increased more than 27.26%. The maximum biomass and maximum specific growth rate of the stimulation group by 8000 Hz, 80dB sound wave was about 1.7 times and 2.5 times that of the control group, respectively. Moreover, it was observed thatE. coliK-12 can respond rapidly to sound stress at both the transcriptional and posttranscriptional levels by promoting the synthesis of intracellular RNA and total protein. Some potential mechanisms may be involved in the responses of bacterial cells to sound stress.

Author(s):  
Eka Indah Raharjo ◽  
Hastiadi Hasan ◽  
Darmawan .

ABSTRAK Penelitian bertujuan untuk mengetahui pergantian pakan. penelitian menggunakan Rancangan Acak Lengkap yang terdiri dari 5 perlakuan dan 3 ulangan. Susunan perlakuan adalah sebagai berikut : Perlakuan A : eguchi 10 hari dan infusoria 10 hari, Perlakuan B : infusoria 10 hari dan eguchi 10 hari, Perlakuan C : Eguchi 20 hari, Perlakuan D : Infusoria 20 hari dan Perlakuan E : campuran eguchi 50% dan Infusoria 50% selama 20 hari. Penelitian ini dilakukan selama 20 hari untuk mengetahui laju pertumbuhan spesifik bobot, laju pertumbuhan mutlak panjang, kelangsungan hidup dan kualitas air sebagai data penunjang. Hasil penelitian menunjukkan pergantian pakan alami dengan pakan buatan menghasilkan respon yang baik dalam pertumbuhan larva ikan gurami. Pergantian pakan buatan (eguchi) dengan pakan alami (infusoria)(perlakuan A) memberikan hasil terbaik dengan rata – rata laju pertumbuhan spesifik bobot 5,3256%, rata – rata laju pertumbuhan mutlak panjang 5.70 mm dan rata – rata kelangsungan hidup 78.89%.  Kata Kunci : Infusoria, Eguchi, Larva Ikan Gurami, Laju Pertumbuhan ABSTRACT Research was aimed to determine the change of feed. research using completely randomized design consisting of 5 treatments and 3 replications. The composition of the treatment is as follows: Treatment A: Eguchi 10 days and infusoria 10 days, Treatment B: infusoria 10 days and Eguchi 10 days, Treatment C: Eguchi 20 days, Treatment D: Infusoria 20 days and Treatment E: a mixture of Eguchi 50% and infusoria 50% for 20 days. This research was conducted for 20 days to determine the specific growth rate weight, the absolute growth rate of long survival and quality of water as supporting data. The results showed turnover natural feed with artificial feed to produce a good response in the growth of larval carp. Substitution of artificial feed (Eguchi) with natural food (infusoria) (treatment A) gives the best results with the average - average specific growth rate 5.3256% weighted, averageabsolute growth rate, average length of 5.70 mm and survival rate 78.89% . Keywords : Infusoria, Eguchi, Fish larvae Gurame, Growth Rate


Omni-Akuatika ◽  
2018 ◽  
Vol 14 (2) ◽  
Author(s):  
Arifianto Heraedi ◽  
Slamet Budi Prayitno ◽  
Tristiana Yuniarti

The thyroxin hormone plays an important role in the process of metabolism, yolk sac absorbsion, and growth of fish. The aims of this research were to observe the effect of various concentration of thyroxin (T4) on the absolute growth weight, total length, survival rate, and pigment development of pink zebra fish larvae (Brachydanio reiro ) after being reared 42 days. The pink zebra fish larvae at 4 day age with the average weight of  0,002 – 0,003g and average length of ± 3.10 – 3.43 mm were immersed at various concentration of T4 for 24 hours. Prior the treatment fish larvae were dipped into 1 ppt salinity for 2 minutes then transferred into 1 Liter, 1ppt saline and various T4 in plastic bags. The thyroxin concentrations were A (0 mg / L); B (0.05 mg / L); C (0.10 mg / L) and D (0.15 mg / L) respectively. The stocking density was 40 fish/L. After that they were transferred into aquariums and reared for 42 days. Completely Randomized Design (RAL) with four treatments and three replications were used. The variables observed were absolute and specific growth, total length, survival rate and hue degree. The results showed that the thyroxin hormone had significant effect on absolute growth weight, total length and specific growth rate.  The dosage 0.1 mg/L was the best treatment on absolute growth, total length, and specific growth rate. Whilee the survival rate showed no significant differences across the treatments. Treatment C also demonstrated the best ppigment development (14.60 + 0.36º hue) compared to others.


Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1637-1648 ◽  
Author(s):  
Julian Ihssen ◽  
Thomas Egli

In batch cultures of Escherichia coli, the intracellular concentration of the general stress response sigma factor RpoS typically increases during the transition from the exponential to the stationary growth phase. However, because this transition is accompanied by complex physico-chemical and biological changes, which signals predominantly elicit this induction is still the subject of debate. Careful design of the growth environment in chemostat and batch cultures allowed the separate study of individual factors affecting RpoS. Specific growth rate, and not cell density or the nature of the growth-limiting nutrient, controlled RpoS expression and RpoS-dependent hydroperoxidase activity. Furthermore, it was demonstrated that the standard E. coli minimal medium A (MMA) is not suitable for high-cell-density cultivation because it lacks trace elements. Previously reported cell-density effects in chemostat cultures of E. coli can be explained by a hidden, secondary nutrient limitation, which points to the importance of medium design and appropriate experimental set-up for studying cell-density effects.


2017 ◽  
Vol 12 (2) ◽  
pp. 53
Author(s):  
Vitas Atmadi Prakoso ◽  
Kurniawan Kurniawan

Inefficient feed management strategy in aquaculture will increase the fish production cost. One of the most effective strategies to solve this problem is through a better understanding of the compensatory growth of cultured fish. O. niloticus BEST tilapia strain (total length: 7.23 ± 0.11 cm mean ± SD; Body weight: 7.04 ± 0.08 g mean ± SD) were reared in aquariums at 26.3 ± 1.4oC for 10 weeks. During the experiment, the control group was fed twice a day. The other two groups were deprived of food for one and two weeks and then fed twice a day during refeeding period. At the end of the experiment, the fish deprived for one week had a body weight, biomass and specific growth rate that were not significantly different from the control group. The body weight, biomass and specific growth rate of fish deprived for two weeks were significantly lower than the other groups. This study revealed that concentrations of ash and lower concentrations of protein and lipid on the deprived groups were higher compared to those without feed deprivation. Mortality of fish was lower than 9% and not significantly different among the treatments. Fish aggressive behavior was the main reason for injuries and death. Given the results, BEST tilapia strain was only able to reach complete growth compensation not longer than one week deprivation period. The results of the present study could be applied as basic information for further research on feeding management of BEST tilapia strain. 


2009 ◽  
Vol 60 (2) ◽  
pp. 183 ◽  
Author(s):  
Eva B. Thorstad ◽  
Sven E. Kerwath ◽  
Colin G. Attwood ◽  
Finn Økland ◽  
Christopher G. Wilke ◽  
...  

Fish telemetry is increasingly used to study fish behaviour in marine systems and it is crucial that the tagging does not affect fish behaviour and welfare negatively. Hence, the long-term effects of surgically implanted acoustic telemetry transmitters on survival, tag retention, healing and growth on Pomatomus saltatrix (known as tailor in Australia, elf in South Africa and bluefish in North America) were studied over 144 days. P. saltatrix are well suited for transmitter implants because no tagging-related short- or long-term mortality or transmitter expulsion was recorded. Small transmitters (9 × 28 mm, 3.3 g in water) did not affect the specific growth rate of tagged fish compared with an untagged control group. In contrast, the fish tagged with large transmitters (13 × 50 mm, 6.9 g in water) had a reduced specific growth rate. The small, but not the large transmitter, seemed suitable for tagging P. saltatrix of the body sizes represented in this study (215–621 g). The specific growth rate was negatively affected by increasing the transmitter-mass-in-water to body-mass ratio. It is recommended that this ratio should not exceed 1.4%.


2015 ◽  
Vol 11 (4) ◽  
pp. 1184-1193 ◽  
Author(s):  
Karl Peebo ◽  
Kaspar Valgepea ◽  
Andres Maser ◽  
Ranno Nahku ◽  
Kaarel Adamberg ◽  
...  

Allocation of protein expression costs between metabolic processes in slow and fast growingE. coliin defined rich medium.


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