scholarly journals Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis

PeerJ ◽  
2019 ◽  
Vol 6 ◽  
pp. e6228 ◽  
Author(s):  
Andrés Montoya ◽  
Manuel Carlos López ◽  
Ivan D. Vélez ◽  
Sara M. Robledo
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Differential Expression ◽  
Calcium Binding ◽  
Therapeutic Response ◽  
Label Free ◽  
Early Biomarkers ◽  
Parasitological Diagnosis ◽  
Pcr Rflp ◽  
Before And After ◽  
Globally Distributed

Background Leishmaniasis is a parasitic disease caused by more than 20 species of the Leishmania genus. The disease is globally distributed and is endemic in 97 countries and three territories in the tropical and subtropical regions. The efficacy of the current treatments is becoming increasingly low either due to incomplete treatment or resistant parasites. Failure of treatment is frequent, and therefore, the search for early biomarkers of therapeutic response in cutaneous leishmaniasis (CL) is urgently needed. Objective The aim of this study was to compare the proteomic profiles in patients with CL before and after 7 days of treatment and identify early biomarkers of curative response. Methods Four patients with a parasitological diagnosis of leishmaniasis with confirmation of species by PCR-RFLP were recruited. All patients had a single lesion, and a protein from the middle of the ulcer was quantified by liquid chromatography and mass spectrometry. Results A total of 12 proteins showed differential expression in the comparative LC-electrospray ionization MS/MS (LC-ESI-MS/MS) triplicate analysis. Seven of them were up-regulated and five of them were down-regulated. Calcium binding proteins A2, A8, and A9 and hemoglobin subunits alpha-2 and delta showed high correlation with epidermis development and immune response. Conclusion We identified changes in the profiles of proteins that had a positive therapeutic response to the treatment. The proteins identified with differential expression are related to the reduction of inflammation and increased tissue repair. These proteins can be useful as biomarkers for early monitoring of therapeutic response in CL.

scholarly journals Computed Tomography and Bronchoscopy in Endobronchial Tuberculosis

2003 ◽  
Vol 10 (8) ◽  
pp. 445-448 ◽  
Author(s):  
Halil Yanardag ◽  
Cüneyt Tetikkurt ◽  
Seza Tetikkurt ◽  
Sabriye Demirci ◽  
Tuncer Karayel
Keyword(s):  
Computed Tomography ◽  
Retrospective Study ◽  
Therapeutic Response ◽  
Histopathological Examination ◽  
Computed Tomographic ◽  
Before And After ◽  
Endobronchial Tuberculosis ◽  
Age Range

BACKGROUND: The therapeutic response to endobronchial tuberculosis is usually evaluated by bronchoscopy. Currently, there are no published studies investigating the use of computed tomography for the evaluation of therapeutic response in endobronchial tuberculosis.OBJECTIVE: A retrospective study was performed to evaluate the bronchoscopic and computed tomographic features of endobronchial tuberculosis before and after treatment. The aim of this study was to investigate the usefulness of computed tomography for the assessment of treatment.METHODS: The clinical, pathological and bronchoscopic features of endobronchial tuberculosis were evaluated in 55 patients. The age range of the patients was 21 to 52 years. Computed tomography and bronchoscopy were performed before and after treatment.RESULTS: Diagnosis of tuberculosis was confirmed by culture and histopathological examination. Bronchoscopic examination revealed 89 endobronchial lesions of various types in 55 patients. The exudative type was the most common. Follow-up bronchoscopy revealed that exudative-, ulcerative- and granular-type lesions healed completely. Computed tomography performed after treatment correlated well with the follow-up bronchoscopic findings.CONCLUSION: The results suggest that follow-up computed tomography is useful for the evaluation of therapeutic response and complications associated with endobronchial tuberculosis, and may replace bronchoscopy.

scholarly journals Immunochemotherapy in American cutaneous leishmaniasis: immunological aspects before and after treatment

2001 ◽  
Vol 96 (1) ◽  
pp. 89-98 ◽  
Author(s):  
VPCP Toledo ◽  
W Mayrink ◽  
KJ Gollob ◽  
MAP Oliveira ◽  
CA da Costa ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
American Cutaneous Leishmaniasis ◽  
Before And After

scholarly journals Expression of IL-1 Family Cytokines in Patients Before and After Cutaneous Leishmaniasis Cure

2021 ◽  
Author(s):  
Ester Amorim ◽  
Marton Kaique Andrade Cavalcante ◽  
Ailton Alvaro Silva ◽  
Vanessa Lucília Silveira Medeiros ◽  
Maria Edileuza Felinto Brito ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Immune Response ◽  
Cutaneous Leishmaniasis ◽  
Mechanisms Of Resistance ◽  
Marked Inhibition ◽  
Gene And Protein Expression ◽  
Th2 Responses ◽  
Before And After ◽  
Response Profiles ◽  
Effector Immune Response

Abstract Cutaneous leishmaniasis is an infectious disease that presents an immune response marked by the activation of lymphocytes and production of cytokines, including those of the IL-1 family, which act as an important trigger for the activation of an effector immune response. Despite this, inflammation exacerbation is sometimes also attributed to IL-1 cytokines, although some others down-regulate inflammation or produce Th2 responses, which need to be further clarified in the CL. Assessing the gene and protein expression of IL-1 cytokines associated with different immune response profiles in PBMCs from patients with active and healed lesions, this study demonstrated that stimulation by L. braziliensis positively regulates inflammatory and anti-inflammatory IL-1 cytokines, as IL-1α/β and IL-37, while there was a marked inhibition of IL-1Ra and IL-18 genes in patients treated with antimony, which perhaps contributes to the mechanisms of resistance that control Leishmania infection.

scholarly journals Integration of mRNA and miRNA Analysis Reveals the Molecular Mechanism of Cotton Response to Salt Stress

2021 ◽  
Vol 12 ◽  
Author(s):  
Jingjing Zhan ◽  
Yangyang Diao ◽  
Guo Yin ◽  
Muhammad Sajjad ◽  
Xi Wei ◽  
...  
Keyword(s):  
Salt Stress ◽  
Differential Expression ◽  
Signal Transduction Pathway ◽  
Differentially Expressed ◽  
Sequencing Data ◽  
Regulatory Relationship ◽  
Significant Differential Expression ◽  
Amplification Bias ◽  
Before And After ◽  
Increased Sensitivity

To identify the regulatory network of known and novel microRNAs (miRNAs) and their targets responding to salt stress, a combined analysis of mRNA libraries, small RNA libraries, and degradome libraries were performed. In this study, we used unique molecular identifiers (UMIs), which are more sensitive, accurate, and reproducible than traditional methods of sequencing, to quantify the number of molecules and correct for amplification bias. We identified a total of 312 cotton miRNAs using seedlings at 0, 1, 3, and 6 h after NaCl treatment, including 80 known ghr-miRNAs and 232 novel miRNAs and found 155 miRNAs that displayed significant differential expression under salt stress. Among them, fifty-nine differentially expressed miRNAs were simultaneously induced in two or three tissues, while 66, 11, and 19 were specifically expressed in the roots, leaves, and stems, respectively. It is indicated there were different populations of miRNAs against salt stress in roots, leaves and stems. 399 candidate targets of salt-induced miRNAs showed significant differential expression before and after salt treatment, and 72 targets of 25 miRNAs were verified by degradome sequencing data. Furthermore, the regulatory relationship of miRNA-target gene was validated experimentally via 5′RLM-RACE, proving our data reliability. Gene ontology and KEGG pathway analysis found that salt-responsive miRNA targets among the differentially expressed genes were significantly enriched, and mainly involved in response to the stimulus process and the plant hormone signal transduction pathway. Furthermore, the expression levels of newly identified miRNA mir1 and known miRNAs miR390 and miR393 gradually decreased when subjected to continuous salt stress, while overexpression of these miRNAs both increased sensitivity to salt stress. Those newly identified miRNAs and mRNA pairs were conducive to genetic engineering and better understanding the mechanisms responding to salt stress in cotton.

scholarly journals Intestinal helminthiasis as a relevant factor on immunological markers status of cutaneous leishmaniasis patients

2020 ◽  
Author(s):  
ALEIDA VERDUGUEZ-ORELLANA ◽  
Ernesto Rojas Cabrera ◽  
Mery Arteaga Terrazas ◽  
Fidelia Barrientos Laura ◽  
Miguel Guzman-Rivero
Keyword(s):  
Immune Response ◽  
Cutaneous Leishmaniasis ◽  
Sand Fly ◽  
Intestinal Helminths ◽  
Sanitation Services ◽  
Immunological Markers ◽  
Tnf Α ◽  
Before And After ◽  
The Status ◽  
Basic Sanitation

Abstract Background: The intestinal helminths and the tegumentary leishmaniasis are frequent in rain forest area of Bolivia by absence of basic sanitation services and the natural presence of sand fly, vector of Leishmania parasites. Each one of these infections triggers a specific immune response by the host, nevertheless there is scant information regarding the behaviour of immune response during simultaneous Leishmania and intestinal helminths infections. The purpose of this study was contributing to the knowledge on this matter. Results: Forty-four cutaneous leishmaniasis patients and thirty controls entered in the study. The percentage of eosinophils from peripheral blood and plasma concentration of IgE and TNF-α were increased at after antimony treatment, respect the values presented before treatment in Albendazole and Non Albendazole groups, those increases were not statically significant. A decrease between before and after antimony treatment was observed in the ratio CD4/CD8 in both groups, but only in the receiving Albendazole group it was presented differences statistically significant.Conclusions: Several changes in the status of immunological markers including the number of eosinophils were found after the antimony treatment of patients. No additional clinical, neither immunological benefit of Albendazole treatment could be documented probably because the contact of individuals with helminths is long-standing.

scholarly journals Basal differences in the transcriptional profiles of tomato leaves associated with the presence/absence of the resistance gene Mi-1 and changes in these differences after infestation by the whitefly Bemisia tabaci

2019 ◽  
Vol 110 (4) ◽  
pp. 463-479
Author(s):  
Clara I. Rodríguez-Alvarez ◽  
Irene López-Vidriero ◽  
José M. Franco-Zorrilla ◽  
Gloria Nombela
Keyword(s):  
Differential Expression ◽  
Resistance Gene ◽  
Bemisia Tabaci ◽  
Plant Resistance ◽  
Tomato Genome ◽  
Transcriptional Profiles ◽  
Resistance To Insects ◽  
Before And After ◽  
Genome Array ◽  
Microarray Analyses

AbstractThe tomato Mi-1 gene mediates plant resistance to whitefly Bemisia tabaci, nematodes, and aphids. Other genes are also required for this resistance, and a model of interaction between the proteins encoded by these genes was proposed. Microarray analyses were used previously to identify genes involved in plant resistance to pests or pathogens, but scarcely in resistance to insects. In the present work, the GeneChip™ Tomato Genome Array (Affymetrix®) was used to compare the transcriptional profiles of Motelle (bearing Mi-1) and Moneymaker (lacking Mi-1) cultivars, both before and after B. tabaci infestation. Ten transcripts were expressed at least twofold in uninfested Motelle than in Moneymaker, while other eight were expressed half or less. After whitefly infestation, differences between cultivars increased to 14 transcripts expressed more in Motelle than in Moneymaker and 14 transcripts less expressed. Half of these transcripts showed no differential expression before infestation. These results show the baseline differences in the tomato transcriptomic profile associated with the presence or absence of the Mi-1 gene and provide us with valuable information on candidate genes to intervene in either compatible or incompatible tomato–whitefly interactions.

scholarly journals Differential expression of polyamine biosynthetic pathways in skin lesions and in plasma reveals distinct profiles in diffuse cutaneous leishmaniasis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hayna Malta-Santos ◽  
Jaqueline França-Costa ◽  
Amanda Macedo ◽  
Artur T. L. Queiroz ◽  
Kiyoshi F. Fukutani ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Differential Expression ◽  
Skin Lesions ◽  
Biosynthetic Pathways

Differential expression of the EF-hand calcium-binding protein calsensin in the central nervous system of hirudinid leeches

1996 ◽  
Vol 286 (3) ◽  
pp. 357-364 ◽  
Author(s):  
Mark Veldman ◽  
Yueqiao Huang ◽  
John Jellies ◽  
Kristen M. Johansen ◽  
Jørgen Johansen
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Differential Expression ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Ef Hand ◽  
The Central Nervous System

scholarly journals Quantitative Analysis of the Seminal Plasma Proteome in Secondary Hypogonadism

2019 ◽  
Vol 8 (12) ◽  
pp. 2128 ◽  
Author(s):  
Giuseppe Grande ◽  
Federica Vincenzoni ◽  
Francesca Mancini ◽  
Ferran Barrachina ◽  
Antonella Giampietro ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Seminal Plasma ◽  
Control Group ◽  
Testosterone Replacement Therapy ◽  
Male Hypogonadism ◽  
Grey Zone ◽  
Label Free ◽  
Clinical Markers ◽  
Before And After ◽  
Secondary Hypogonadism

In the grey zone of testosterone levels between 8 and 12 nmol/L, the usefulness of therapy is controversial; as such, markers of tissue action of androgens may be helpful in adjusting clinical decisions. To better understand the effect of the hypothalamic-pituitary-testicular axis on male accessory secretion, we performed a proteomic quantitative analysis of seminal plasma in patients with secondary hypogonadism, before and after testosterone replacement therapy (TRT). Ten male patients with postsurgical hypogonadotrophic hypogonadism were enrolled in this study, and five of these patients were evaluated after testosterone treatment. Ten men with proven fertility were selected as a control group. An aliquot of seminal plasma from each individual was subjected to an in-solution digestion protocol and analyzed using an Ultimate 3000 RSLC-nano HPLC apparatus coupled to a LTQ Orbitrap Elite mass spectrometer. The label-free quantitative analysis was performed via Precursor Ions Area Detector Node. Eleven proteins were identified as decreased in hypogonadic patients versus controls, which are primarily included in hydrolase activity and protein binding activity. The comparison of the proteome before and after TRT comes about within the discovery of six increased proteins. This is the primary application of quantitative proteomics pointed to uncover a cluster of proteins reflecting an impairment not only of spermatogenesis but of the epididymal and prostate epithelial cell secretory function in male hypogonadism. The identified proteins might represent putative clinical markers valuable within the follow-up of patients with distinctive grades of male hypogonadism.

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