scholarly journals Evaluation of IgG Antibody Responseto Severe Acute Respiratory Syndrome Coronavirus-2 in Healthcare Workers in aTertiary Care Centre, Chennai, India

2021 ◽  
Author(s):  
Therese Mary Dhason ◽  
Suganthi Manikesi ◽  
Usha Krishnan ◽  
Vasant Hamani ◽  
. Hemalatha ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Healthcare Workers ◽  
Statistical Significance ◽  
Enzyme Linked Immunosorbent Assay ◽  
P Value ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
Igg Antibody ◽  
Chi Square ◽  
Chi Square Test

Introduction: Globally, the Coronavirus Disease-2019 (COVID-19) pandemic poses a high risk for Healthcare Workers (HCWs) who are among the population that is most vulnerable of being infected with Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2). With a prevailing pandemic such as COVID-19, it becomes important to understand the presence and persistence of antibodies in the serum of HCW, testing positive for COVID-19 on Reverse transcriptase Polymerase Chain Reaction (RT-PCR). An understanding of the prevalence of IgG antibodies against COVID-19 and the duration for which they are present in the serum will help in predicting the immune response of individuals against the disease. Aim: To study the prevalence of COVID-19 IgG antibodies in laboratory confirmed COVID-19 RT-PCR positive symptomatic, asymptomatic and RT-PCR negative subjects. Materials and Methods: The present longitudinal study was conducted from April to December 2020 with a sample size of 90 participants based on a pilot study. Blood sample was collected and serum was separated. Enzyme Linked Immunosorbent Assay (ELISA) was done to detect the presence of COVID-19 IgG antibody in serum. Chi-square test and Pearson correlation were used to find out the statistical significance of COVID-19 IgG antibodies in COVID-19 positive and negative HCWs and the relationship between Cycle threshold values (Ct) and antibody levels, respectively. Results: Fever with sore throat was the most common (33%) symptom. Chi-square test done to compare IgG among RT-PCR positive and negative subjects showed p-value of <0.0001 which was significant. However, statistical significance was not found (p-value 0.9973) with respect to COVID-19 IgG antibodies in RT-PCR positive COVID-19 asymptomatic and symptomatic subjects. Mean antibody index in symptomatic and asymptomatic was 3.7743±1.9834 and 3.571±1.7961, respectively. Average number of days, the antibodies persisted was 25 days-266 days. Conclusion: The prevalence of COVID-19 antibodies among RT-PCR positive symptomatic, asymptomatic and RT-PCR negative subjects was 87.8%, 66.7% and 26.7%, respectively. The maximum number of days antibodies persisted was 266days. Further studies will elucidate whether these antibodies prevent re-infection.

scholarly journals Gloves Perforations in Minor Oral Surgical Procedures: A Comparison of Latex and Nitrile Gloves

2021 ◽  
Vol 30 (02) ◽  
pp. 103-106
Author(s):  
Ajeet Kumar ◽  
◽  
Naveed Iqbal ◽  
Javaria Farooq ◽  
Saad Uddin Siddiqui
Keyword(s):  
Local Anesthesia ◽  
Surgical Procedures ◽  
Statistical Significance ◽  
Descriptive Analysis ◽  
P Value ◽  
Dominant Hand ◽  
Chi Square ◽  
Oral Surgical Procedures ◽  
Latex Gloves ◽  
Chi Square Test

OBJECTIVE: This study was performed to compare incidence of perforations in Latex and Nitrile examination gloves during Minor Oral surgical procedures performed under local anesthesia. METHODOLOGY: 100 pairs of latex and 100 pairs of Nitrile examination gloves where used to perform 200 minor oral surgical procedures under local anesthesia. After completion of every minor oral surgical procedure each gloves was examined by Water Inflation method to observe presence or absence of Perforations. A data sheet was used to collect data including type of Gloves used (Latex or Nitrile) presence of perforations, sites of perforations and nature of minor oral surgical procedures. Data was entered and analyzed using SPSS version 20. Descriptive analysis was conducted to calculate frequency and percentages of Number and sites of perforations for both Nitrile and latex examination gloves. Chi Square test was used to find out statistical significance of difference of perforations rate between Nitrile and Latex gloves. P value of < 0.05 was considered significant. RESULTS: Out of 200 latex gloves 23 (11.5) had 29 perforations whereas out of total 200 Nitrile gloves 28 (14%) had 22 perforations. Nitrile gloves had a statistically significant higher rate of perforations as compared to Latex gloves. (P value 0.043). For both Nitrile and Latex gloves left non dominant hand had highest frequency of perforations Latex 18 (81.81%) perforations and Nitrile 18(62.06%). Index finger and thumb were most frequent sites of perforations in both Latex and Nitrile gloves. CONCLUSION: Gloves perforations were more common in Nitrile examination gloves however total number of perforations was more in Latex examination gloves.

scholarly journals ULTRASOUND PREDICTABILITY OF LOWER UTERINE SEGMENT CESAREAN SECTION SCAR THICKNESS

2020 ◽  
pp. 9-11
Author(s):  
Madhu Kumari ◽  
Kumari Bibha ◽  
Abha Sinha ◽  
Debarshi Jana
Keyword(s):  
Cesarean Section ◽  
Gestational Age ◽  
Statistical Significance ◽  
P Value ◽  
Chi Square ◽  
Medical College ◽  
Chi Square Test ◽  
Uterine Scar ◽  
Age Range ◽  
The Given

Objective: The objective of this study is to find out association between scar thickness, assessed sonographically, and intraoperative findings (IOF). Study Design: Descriptive study. Place and Duration of Study: Department of Obstetrics and Gynecology, Sri Krishna Medical College and Hospital, Muzaffarpur, Bihar from June 2019 to May 2020. Methodology: A total of 70 pregnant patients were included in this study. Transabdominal ultrasound was done for scarred uteri. Sonographic findings were co-related with introperative findings. All the given data were entered on SPSS version 23. Age was expressed as mean ± SD. Parity, gestational age, and interval between cesarean sections were expressed as frequencies with percentages. Statistical analysis was done by using Chi-square test for categorical data for association between sonographic scar thickness and intraoperative findings. The statistical significance was set at p-value <0.05. Results: The age range of the patients was 20- 36 years with a mean of 27.91 ±3.690 years. Gestational age at the time of cesarean section was between 27-40 weeks of gestation with a mean of 37 ±2.126 weeks. The interval from previous cesarean was 10 months at the minimum, and 6 years at the maximum with a mean of 2.29 ±1.0 months. Mean scar thickness was 2.5 mm. Association between scar thickness (<1-3 mm) and intaoperative findings of dehiscence and rupture showed a p-value of <0.001. Conclusion: Sonographic assessment of a uterine scar has a practical application to determine the thickness of previous scar, and assess its integrity.

scholarly journals Incidences of Root Microcracks Caused by Hand and Rotary File System at Different Lengths

2019 ◽  
Vol 3 (9) ◽  
pp. 283-289
Author(s):  
Shuchi Sukul ◽  
Goldy Rathee ◽  
Parimal Anand ◽  
Sakshi Kataria ◽  
Pratibha Taneja
Keyword(s):  
File System ◽  
Statistical Significance ◽  
File Systems ◽  
In Vitro Study ◽  
Root Surface ◽  
P Value ◽  
Chi Square ◽  
Chi Square Test ◽  
Group B

OBJECTIVES: The present research was conducted to assess incidences of root microcracks caused by hand and rotary file system at different lengths MATERIAL AND METHODS: This in-vitro study was undertaken to assess incidence of root microcracks caused by hand and rotary file system at different lengths In total, 100 the mandibular premolar with straight roots determined with intact, fully formed apices were taken. Samples were randomly distributed into 5 groups based on the file system used: a) Group A: Control, b) Group B: Reciproc, c) Group C: WaveOne, d) Group D: One Shape and e) Group E: ProTaper. Pearson Chi-square test was used to determine the differences between groups. The dentinal defects were expressed as percentage of samples with microcracks in each group. Level of statistical significance was set at p-value less than 0.05. RESULTS: The chi square test was used to compare the Distribution of the number of teeth in which cracks were observed on the horizontal sections. It was found to be significant with group ProTaper showing maximum cracks at 3,6,9 mm level as compared to other file systemCONCLUSION: Nickel-titanium instruments causes cracks on the apical root surface or in the root canal wall. ProTaper causes maximum dentinal cracks as compared to other file systems

scholarly journals Neonatal and Maternal Outcomes in Spontaneously-Conceived Twin Pregnancies According to Mode of Delivery

Folia Medica ◽  
2020 ◽  
Vol 62 (3) ◽  
pp. 468-476
Author(s):  
Samantha Mc Kenzie Stancu ◽  
Manuela Cristina Russu
Keyword(s):  
Neonatal Intensive Care ◽  
Statistical Significance ◽  
Mode Of Delivery ◽  
Statistical Correlation ◽  
P Value ◽  
Maternal Outcomes ◽  
Chi Square ◽  
Chi Square Test ◽  
Nicu Admission ◽  
Twin Pregnancies

Introduction: Appropriate intrapartum conduct in a twin delivery remains a challenging aspect of obstetric practice. The objective of this study was to compare neonatal and maternal outcomes in twin pregnancies according to mode of delivery.&nbsp; Materials and methods: This is a single centre retrospective cohort study of all consecutive spontaneously-conceived twin deliver-ies (&ge; 24 weeks, estimated fetal weight &ge; 500 grams) over a nine-year period between 01/01/2007 - 31/12/2016 at a tertiary-level centre. Neonatal outcomes included survival, APGAR score, prematurity-associated pathology (PAP), admission to the neonatal intensive care unit (NICU) and length of stay (LOS). Maternal outcomes included postpartum complications and LOS. Statistical analysis comprised Chi-square test with subsequent p-value and odds-ratio with 95% confidence interval. Statistical significance was set at p<0.05. Results: A total of 173 consecutive women with spontaneously-conceived twin deliveries were enrolled in this study, 129 (74.6%) women delivered by caesarean section (CS). The success rate of vaginal delivery (VD) was 93.6% (44/47). A strong statistical correlation was identified between CS and NICU admission; 53.2% vs. 1.5% (p=0.0001). Neonatal LOS in the NICU was significantly longer in the CS group. Prematurity-associated pathology (PAP) was noted in 75 pairs of twins (75/173); 61 pairs were delivered by CS, bearing strong statistical significance (p<0.0001). Postpartum complications occurred in 14.7% of CS compared to 13.6% of VDs.&nbsp; Conclusion: Neonates delivered by CS had a higher rate of PAP, NICU admission, lower birth weight and longer LOS. This study showed that VD is safe, especially when the first twin is in cephalic presentation

scholarly journals Foetomaternal outcome in multifoetal pregnancy in a tertiary rural teaching hospital

2021 ◽  
Vol 10 (2) ◽  
pp. 614
Author(s):  
Sneha Gond ◽  
Partha Pratim Sharma ◽  
Narra Madhuri ◽  
Kasturi Barman
Keyword(s):  
Prospective Observational Study ◽  
Statistical Significance ◽  
P Value ◽  
Statistical Association ◽  
Maternal Complications ◽  
Chi Square ◽  
Medical College ◽  
Chi Square Test ◽  
Nicu Admission ◽  
Rural Teaching

Background: Aim of the study was to find out foeto-maternal outcome of multifoetal pregnancy in relation to chorionicity and to analyse the associated risk factors.Methods: This study was a prospective observational study on multifoetal pregnancy conducted at Midnapore medical college and hospital. Statistical analysis was done by chi-square test and statistical significance was set at p-value<0.05.Results: Incidence of multifoetal pregnancy was 1.7%. Modal age group was 20-24 years age and 82% conceived spontaneously. Among chorionicity, Dichorionic diamniotic (DCDA) were 60% followed by 34% Monochorionic diamniotic (MCDA), 4% Monochorionic monoamniotic (MCMA) and 2% Trichorionic triamniotic (TCTA). Among maternal complications preterm labour were present in both twins and triplets followed by anemia. LSCS was required in 60.8 % of twins and common indication was malpresentation. Majority of MC and TC were delivered at gestational age of 31-33 weeks (47.1% MCDA, 50% MCMA and 60% TCTA) and dichorionic delivered at 34-36 week (48.7%). Statistical association between neonatal outcomes and chorionicity were significant in IUGR (p=<0.0001), stillbirth (p=0.0356), congenital anomalies (p=0.0017), discordant growth (p<0.0001), Apgar score <7 (p<0.0001), low birth weight (p=0.014), live birth (p≤0.0001) and NICU admission (p<0.0001).Conclusions: Maternal and perinatal outcome was significant in monochorionic and trichorionic pregnancies compared to dichorionic pregnancies with increased NICU admissions required in monochorionic and trichorionic gestations.

scholarly journals Small dose of ephedrine for prevention of hypotension following propofol and fentanyl administration during induction of general anesthesia

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Gamal Ejaimi ◽  
Abla Saab ◽  
Sittelnissa Ahmed ◽  
Areeg Ahmed ◽  
Hussain Abujamilah ◽  
...  
Keyword(s):  
General Anesthesia ◽  
Small Dose ◽  
Elective Surgery ◽  
Statistical Significance ◽  
Control Group ◽  
P Value ◽  
Chi Square ◽  
Arterial Blood ◽  
Chi Square Test ◽  
Study Participants

Induction of general anesthesia with propofol and fentanyl could result in hypotension and bradycardia. Various methods are being used to prevent these adverse effects. The aim of our study was to assess the efficacy and safety of a small dose of ephedrine in prevention of hypotension following administration of propofol and fentanyl. This prospective, randomized, comparative study was conducted among a total of 50 patients, both genders, age 18 years up to 60 years with ASA grade Ι and ΙΙ and some of class ΙΙΙ, presented for elective surgery under general anesthesia. Patients were randomly allocated into one of two groups (25 patients in each): CG (the control group), which received propofol in a dose of 2 mg/kg, intravenously, over 20–30 s mixed with 2 mL normal saline: and EphG (Ephedrine group), while received propofol in a dose of 2 mg/kg, intravenously, over 20–30 s mixed with 2 mL of ephedrine (10 mg). The Mean Arterial Blood Pressure (MAP) and Heart Rate (HR) were recorded before induction and then every 1 min up to 6 min after induction. The categorical data are presented as a number and percentage and were subjected to Fisher’s exact or Chi-square test for analysis. The statistical significance was p≤0.05. The significant differences in HR were observed in the 3rd,4th, and 5th minutes with P-value, 0.018, 0.000, 0.000, respectively. However, no patient in the study participants had bradycardia. The significant differences in MAP were observed in the 2nd, 3rd,4th, and 5th minutes with P-value, 0.035, 0.000, 0.000, and 0.000, respectively. The percentage of patient in CG who developed hypotension in the 3rd and 4th is 44% and 32% compared to 8% and 0% in EphG, with significant differences (P-value 0.004 and 0.002, respectively). Administration of small dose of ephedrine with propofol could attenuate propofol/fentanyl hypotensive and bradycardic effects.

scholarly journals Seroprevalence of Chikungunya (CHIK) Infection during Non Epidemic Periods in Chennai, Southern India

2021 ◽  
Author(s):  
Kaliyamoorthy Kayalvili ◽  
Murugan Duraivel ◽  
Elangovan Nivedhitha ◽  
Subramanian Arul Selvan
Keyword(s):  
Disease Transmission ◽  
Statistical Significance ◽  
Cross Sectional Study ◽  
Southern India ◽  
Enzyme Linked Immunosorbent Assay ◽  
P Value ◽  
Chi Square ◽  
Monsoon Period ◽  
Cross Sectional ◽  
Igm Antibody

Introduction: Chikungunya (CHIK) infection has caused many outbreaks in India with more than 13 lac people affected by the disease. Epidemics of CHIK infection occur during post monsoon period when there is a high vector density. Evidences on the prevalence of CHIK infection during non epidemic periods are limited. Aim: To determine the seroprevalence of CHIK infection during non epidemic periods among patients attending fever clinic in Chennai, Southern India. Materials and Methods: This cross-sectional study was done in 180 suspected cases of CHIK infection between the months of September 2014 and February 2015. A 5 mL of blood samples were collected from the suspected cases and serum was separated to detect for the presence of CHIK-IgM antibody by using CHIK-IgM antibody capture Enzyme Linked Immunosorbent Assay (ELISA) kit. Chi-square test was done to find out the statistical significance with p-value <0.05 kept as statistically significant. Results: The seroprevalence of CHIK infection during non epidemic periods was found to be 5.5% (10/180). Fever and joint pain were the major complaints present in all the study population. All the seronegative cases were tested for the presence of other infections and it was found that 13% were positive for typhoid, 9% were positive for leptospirosis, 4% for malaria and 2% for dengue infections. Conclusion: There are no vaccines or specific medications available till date. Prevention is the only effective approach against the disease. Even though the prevalence of CHIK infection is low during non epidemic periods, strict vector control and elimination of mosquito breeding sites are very important in controlling the disease transmission.

scholarly journals NASOPHARYNGEAL SPECIMEN COLLECTION FROM SUSPECTED COVID-19 CASES, MAKING THE PROCEDURE SAFE

2021 ◽  
Vol 71 (Suppl-3) ◽  
pp. S432-36
Author(s):  
Sohail Aslam ◽  
Syed Muhammad Asad Shabbir Bukhari ◽  
Adnan Asghar ◽  
Naeem Riaz ◽  
Iftekhar Yousaf ◽  
...  
Keyword(s):  
Standard Technique ◽  
Control Group ◽  
P Value ◽  
Nasopharyngeal Swab ◽  
Sample Collection ◽  
Rt Pcr ◽  
Modified Technique ◽  
Chi Square ◽  
Chi Square Test ◽  
The Difference

Objective: To comparison was made between standard (control group) nasopharyngeal sample collection technique for RTPCR and modified technique and the outcome was compared in terms of the proportion of positive results of Rt-PCR tests. Study Design: Double blinded randomized clinical trial. Place and Duration of Study: Pakistan Naval Ship Shifa Hospital Karachi Pakistan, from June and July 2020. Methodology: This study was a newly developed modified technique for nasopharyngeal sampling for RT-PCR tests of COVID-19 suspects. Target population included all patients who developed COVID-19 related symptoms and/or also had history of recent travel or closed contact with Covid-19 patients. Total 1500 nasopharyngeal PCR tests were done by a team of trained technicians. Systemetic probability sampling technique was utilized. Subjects were divided into two groups by using even and odd serial numbers. Proportion of positive test results were compared between two groups by using chi square test. Results: Results were collected for 3000 nasopharyngeal swab sample for RT-PCR testing. Mean age was 31.68 ± 11.89 years. In study group with modified technique, 470 tests were found positive for a total of 1500 samples while only 297 out of 1500 samples were detected positive in control group with standard technique. Chi square test applied to assess the difference between this proportion and it proved that the difference was highly statistically significant (p-value <0.00). Conclusion: we interpret that modified samples collection technique is relatively safe for sample collector of Covid-19 PCR which has got potential benefits to get more genuine results of these......

scholarly journals The Correlation Between IgM and IgG Antibodies With Blood Profile In Patients Infected With COVID 19

2021 ◽  
Author(s):  
Zahra Alibolandi ◽  
Amirreza Ostadian ◽  
Saeed Sayyah ◽  
Hamed Haddad Kashani ◽  
Hassan Ehteram ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Antibody Response ◽  
Laboratory Data ◽  
Severe Illness ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Blood Profile ◽  
Virus Exposure ◽  
Antibody Levels

Abstract Objectives: This study aimed to determine the levels of IgM and IgG antibody response to the severe acute respiratory syndrome coronavirus (SARS-CoV)-2 in coronavirus disease 2019 (COVID-19) patients with different disease severity.Methods: IgM and IgG antibody levels were evaluated via enzyme-linked immunosorbent assay (ELISA). In total, 100 patients with confirmed SARS-CoV-2 infection were enrolled in this study and viral RNA was detected by using Real-time PCR technique. Clinical and laboratory data were collected and analyzed after hospital admission for COVID-19 and two months post-admission. Results: The level of anti-SARS-CoV-2 antibody IgG was significantly higher in the severe patients than those in moderate and mild groups, 2 months after admission. Also, level of IgG was positively associated with increased WBC, NUT and LYM counts in sever than mild or moderate groups after admission to hospital.Conclusion: Our findings suggested that patients with severe illness might experience longer virus exposure times and have a stronger antibody response against viral infection. Thus, they have longer time immunity compared with other groups.

scholarly journals The Level of Awareness and Utilization of Computer Shields among Computer users in a Nigerian Community

2011 ◽  
Vol 1 (2) ◽  
pp. 49-52
Author(s):  
Chidi Oliver Ihemedu ◽  
Charles Oluwole Omolase
Keyword(s):  
Statistical Significance ◽  
Simple Random Sampling ◽  
Computer Users ◽  
Hard Copy ◽  
P Value ◽  
Chi Square ◽  
Ocular Symptoms ◽  
Chi Square Test ◽  
History Of ◽  
On Line

Objective: This study was designed to determine the level of awareness and extent of use of computer shields among computer users in Owo, in South-West Nigeria. Material & Methods: The subjects were selected by a simple random sampling method. Data collection was through a combination of e-mail based on-line questionnaire using the survey monkey website, and the self administered semi structured hard copy questionnaire. The information obtained from the respondents with the questionnaire included their bio-data, extent of computer use, history of ocular symptoms, awareness and utilization of computer shields. The data obtained with the study instrument was collated and analyzed using the SPSS 15.0.1 statistical software, cross tabulation of attendants results were done with chi-square test and statistical significance was set at p ≤ 0.05. Results: 108 of 124 selected subjects filled their questionnaire to acceptable level. There were a large number of respondents who were aware of the various types of computer shields (70.5%) (p-value 0.002). However, the utilization level was not commensurate as only 39.8% (N= 43) utilized any of these shields, 49.1% (N= 53) were none users while 11.1% (N= 12) did not respond (p-value 0.062). Conclusion: Majority of the respondents were aware of computer shield. However, less than half of the respondents utilized computer shields. There is need to encourage all computer users on the use of computer shields to ensure better computer working time. Key Words: Awareness; Computer shields; Nigeria DOI: 10.3126/ajms.v1i2.2826Asian Journal of Medical Sciences 1 (2010) 49-52

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