ASSESSMENT OF SPASMOLYTIC ACTIVITY OF ALCOHOLIC EXTRACT OF ACILLEA MILLEFOLIUM, RUBIA CORDIFOLIA AND SAUSSUREA LAPPA IN WISTAR ALBINO RATS

The suspensions of alcoholic extract of root bark of the plant Calotropis gigantea in 0.6% carboxy methyl cellulose (CMC) were evaluated for hepatoprotective activity in Wistar albino rats by inducing hepatic injury with D-galactosamine (400 mg/kg). Alcoholic extract of root bark of the plant Calotropis gigantea at an oral dose of 200 mg/kg and 400 mg/kg exhibited a significant (P<0.001, P<0.01 and P<0.05) protection effect by normalizing the levels of aspartate amino transferase (ASAT/ GOT), alanine amino transferase (ALAT/GPT), alkaline phosphatase (ALP), total bilirubin (TB), lactate dehydrogenase (LDH), which were significantly (P<0.001) increased in rats by treatment with 400 mg/kg i.p. of D-galactosamine. Silymarin (25 mg/kg), a known hepatoprotective drug used for comparison exhibited significant activity (P<0.001).

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Ameliorative effect of Aerva lanata against nephrolithiasis following chronic administration of Ethylene glycol in male wistar Albino rats

10.21203/rs.3.rs-1102620/v1 ◽

2021 ◽

Author(s):

Ankul Singh S ◽

Gowri K ◽

Chitra V

Keyword(s):

Ethylene Glycol ◽

Urine Volume ◽

Chronic Administration ◽

Negative Control ◽

Albino Rats ◽

Standard Group ◽

Alcoholic Extract ◽

Health Crisis ◽

Group I ◽

Wistar Albino Rats

Abstract Nephrolithiasis appear to be a major health crisis among the population with serious medical related consequences throughout the lifetime of patient. The aim of the study was to evaluate the preventive effect of the hydro-alcoholic extract of A. lanata roots on Urolithiasis rats. Thirty adults male wistar Albino rats weighing 200 – 250 g were divided into five groups comprising 6 rats in each. Group I Served as positive control with water ad libitum. Group II as negative control which is disease treated group receiving 0.75% ethylene glycol mixed with drinking water for 28 days. Group III chosen as standard group receiving ethylene glycol for first 14 days and Cystone 750 mg/Kg from day 15 till day 28. Group IV and V received ethylene glycol for first 14 days and treatment regimen of LD (400 mg/Kg) and HD 800 mg/Kg orally from day 15 till day 28. Invitro studies like Nucleation, Aggregation and Growth assays were performed. Urine volume and pH was collected and observed for change in appearance, pH, odour and turbidity. Extract was given by preparing suspension and stability was observed by measuring its parameters. On Day 29, the kidneys were dissected and histopathology was done to check tubular injury. There was Increase in urine volume, enhanced excretion of urinary constituents like citrate, oxalate etc. and improving clearance rate. Improvement in pH and antioxidant activity was observed in treated groups. The extract showed that it has prominent effect on nephrolithiasis and has better safety profile in the dose given.

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In-vivo hypoglycemic and hypolipidemic properties of Tagetes lucida alcoholic extract in streptozotocin-induced hyperglycemic Wistar albino rats

Annals of Agricultural Sciences ◽

10.1016/j.aoas.2017.11.005 ◽

2017 ◽

Vol 62 (2) ◽

pp. 169-181 ◽

Cited By ~ 3

Author(s):

Samah A. Abdel-Haleem ◽

Abeer Y. Ibrahim ◽

Rasha F. Ismail ◽

Nermeen M. Shaffie ◽

S.F. Hendawy ◽

...

Keyword(s):

Albino Rats ◽

Alcoholic Extract ◽

Wistar Albino Rats

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BURN WOUND HEALING POTENTIAL OF RUBIA CORDIFOLIA LINN ON WISTAR ALBINO RATS

Journal of Biological and Scientific Opinion ◽

10.7897/2321-6328.05253 ◽

2017 ◽

Vol 5 (2) ◽

pp. 17-20

Author(s):

Shilji Devassy ◽

Ravi Mundugaru ◽

Poornima Solapure ◽

S K Hiremath ◽

P A Patil

Keyword(s):

Wound Healing ◽

Albino Rats ◽

Burn Wound ◽

Rubia Cordifolia ◽

Burn Wound Healing ◽

Wistar Albino Rats

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Anti-Hypertensive Activity of Hydro Alcoholic Extract of Cìraka Cūraṇam on High Salt Loaded Wistar Albino Rats

International Journal of Research and Review ◽

10.52403/ijrr.20210516 ◽

2021 ◽

Vol 8 (5) ◽

pp. 110-115

Author(s):

Sujeethasai K ◽

Manoharan A ◽

Santhanakumar M

Keyword(s):

Heart Diseases ◽

Premature Death ◽

Antihypertensive Activity ◽

Albino Rats ◽

Alcoholic Extract ◽

Deoxycorticosterone Acetate ◽

Wistar Albino Rats ◽

Animal House ◽

Hypertensive Activity

Background: Hypertension is called as Silent killer. The most important risk factor for heart diseases and stroke and it may leads to premature death. Several medicinal plants have high effective in anti-hypertensive and anti-thrombotic activity without any side effects. Aim: To evaluate the anti-hypertensive activity of hydro alcoholic extract of Cìraka cūraṇam on deoxycorticosterone acetate (DOCA) salt induced wistar albino rats. Study design: Observational in-vivo study Place and duration of study: Animal house, Dept. of Pharmacology, Arulmigu Kalasalingam College of Pharmacy, Krishnankoil, Srivilliputtur,Tamilnadu. Materials and methods: Antihypertensive activity was conducted on wistar albino rats by determining serum Sodium and Potassium levels by using semi auto analyzer (RA-50, Bayer Diagnostics), using specific kits (Auto span, India) at 500 and 550 nm respectively and left carotid artery (for recording BP) was cannulated under aseptic conditions with polyethylene cannula filled with 1% heparin in normal saline. Rest procedure, which was stated under the 2K1C-model was followed and BP was observed in terms of mm of Hg. Results: The Cìraka cūraṇam possesses strong antihypertensive effect against DOCA-salt hypertensive rats, which is evidenced by a considerable decrease in blood pressures. Keywords: Anti-hypertensive activity, Cìraka cūraṇam, Wistar albino rats, Deoxycorticosterone acetate.

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EVALUATION OF ANTI-ALZHEIMER ACTIVITY OF ALCOHOLIC EXTRACT OF COSTUS PICTUS D. DON LEAVES IN WISTAR ALBINO RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2020.v13i2.36337 ◽

2019 ◽

pp. 36-43

Author(s):

PASHA KHOOSHBU ◽

IMTIYAZ ANSARI

Keyword(s):

Body Weight ◽

Elevated Plus Maze ◽

Albino Rats ◽

Alcoholic Extract ◽

Object Recognition Test ◽

Albino Rat ◽

Y Maze ◽

Plus Maze ◽

Wistar Albino Rats ◽

Phytochemical Studies

Objective: The aim was to assess the effect of anti-Alzheimer’s activity of the alcoholic extract of Costus pictus D. Don Leaves in Alzheimer induce Wistar albino rats. Methods: In the present study, the efficacy of an Extract of Costus pictus D. Don (ECPDD) was evaluated against scopolamine-induced Alzheimer in the Wistar albino rat. Donepezil at a dose of 2.5 mg/kg, ECPDD was given in rat at a dose of 100 mg/kg body weight, 150 mg/kg body weight, and 200 mg/kg body weight. Anti-Alzheimer activity was assessed by victimization novel object recognition test, elevated plus maze, and Y-maze. And by biochemical test like neurotransmitter esterase activity, catalase activity, malonyldialdehyd and glutathione assays. Results: Results showed that extracts treatment prevents Alzheimer and increases the level of acetylcholine, catalase, glutathione peroxidase, and phytochemical studies identified the presence of flavonoid and alkaloid in the ECPDD. Conclusion: According to the results, it was concluded that the leave of C. pictus D. Don has significant anti-Alzheimer activity due to the presence of potent antioxidants such as flavonoid and anticholinesterase enzyme present in alkaloids.

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Effects of Salacia oblonga on aluminium induced changes in membrane ATPases in brain cortex and hematological parameters in Wistar albino rats

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20192115 ◽

2019 ◽

Vol 8 (6) ◽

pp. 1215

Author(s):

Divyashanthi C. M. ◽

Nandhini A. ◽

Barathane Datchanamurty

Keyword(s):

Brain Cortex ◽

Aluminum Toxicity ◽

Hematological Parameters ◽

Combined Treatment ◽

Aluminium Toxicity ◽

Albino Rats ◽

Active Principle ◽

Alcoholic Extract ◽

Salacia Oblonga ◽

Wistar Albino Rats

Background: The current study was done to evaluate the effect of hydro alcoholic extract of Salacia oblonga on aluminum induced toxicity in brain cortex and blood in Wistar albino rats.Methods: The experimental animals were divided into four groups, each group comprising of six animals for 36 days of experimental duration. We investigated Na+/K+, Mg2+, Ca2+ ATPases enzyme activity in brain cortex and hematological changes if any, upon administration of aluminum chloride (Alcl3) (300 mg/kg b.w), hydro alcoholic extract of Salacia oblonga (67 mg/kg b.w) and Alcl3+ Salacia oblanga with control (distilled water).Results: In brain cortex, Salacia caused an increased in activity of ATPases. Combined administration of Salacia suppressed the influence of aluminum on the ATPases in the brain cortex (p<0.05). Both aluminum as well as Salacia did not cause any alteration in the hemoglobin content of blood in Wistar albino rats. The erythrocytes count was also not altered by treatment with either aluminum or Salacia. Combined treatment with Salacia suppressed the influence of aluminum with reference to neutrophil count and significant increases in monocyte as well as lymphocyte count were seen. Thus, repeated administration of aluminum causes a decrease in neutrophil and increase in lymphocyte count.Conclusions: All the ATPases in brain cortex were found to be affected by aluminum administration and Salacia is found to counteract the ATPase effect to a particular extent implying the presence of an active principle that can counteract the aluminium toxicity indicating its possible usefulness in aluminum toxicity.

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Evaluation of antidepressant activity of hydro-alcoholic extract of rhizomes of Nardostachys jatamansi DC per se and in combination with fluoxetine in wistar albino rats and swiss albino mice

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20195761 ◽

2019 ◽

Vol 9 (1) ◽

pp. 32

Author(s):

Radhika Panchal ◽

Nutanbala N. Goswami

Keyword(s):

Forced Swimming Test ◽

Antidepressant Activity ◽

Forced Swimming ◽

Albino Rats ◽

Alcoholic Extract ◽

Nardostachys Jatamansi ◽

Swimming Test ◽

Per Se ◽

Wistar Albino Rats ◽

Dose Dependent

Background: Depression is a common mental disorder results due to deficiency of neurotransmitter in the brain. Various medicinal properties of jatamansi are mentioned in Ayurveda. This study evaluated effect of hydro-alcoholic extract of rhizomes of Nordostachys jatamansi DC per se and in combination with fluoxetine in wistar albino rats and swiss albino mice.Methods: Animals of either sex were selected and randomly divided in test group. Jatamansi extract 10:1 and fluoxetine hydrochloride dissolved in distilled water were used. Animals were tested for forced swimming test, tail suspension test and locomotor after given test drug. Results were compared with control and analysed.Results: Nardostachys jatamansi DC, when given to rats showed dose dependent increase in number of rotation during forced swimming test in rats. During forced swimming test in glass jar statistically significant decrease in immobility was observed. Nardostachys jatamansi DC, when given to mice dose dependent statistically significant decrease in immobility time, swimming time and climbing observed. When given along with combination of fluoxetine it shows statistically significant difference in result, confirmed that it can have synergistic antidepressant activity. When used for locomotor activity in mice none of the test drugs significantly increase or decrease the locomotor activity.Conclusions: Jatamansi showed antidepressant like property in various tests conducted on rats and mice. It showed statistically significant result with increasing dose and had synergic effect when given along with fluoxetine.

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Analgesic and Anti-Inflammatory Activity ofPinus roxburghiiSarg.

Advances in Pharmacological Sciences ◽

10.1155/2012/245431 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 18

Author(s):

Dhirender Kaushik ◽

Ajay Kumar ◽

Pawan Kaushik ◽

A. C. Rana

Keyword(s):

Diclofenac Sodium ◽

Inflammatory Activity ◽

Bark Extract ◽

Albino Rats ◽

Alcoholic Extract ◽

Pinus Roxburghii ◽

Paw Oedema ◽

Anti Inflammatory ◽

Wistar Albino Rats ◽

Chir Pine

The Chir Pine,Pinus roxburghii, named after William Roxburgh, is a pine native to the Himalaya.Pinus roxburghiiSarg. (Pinaceae) is traditionally used for several medicinal purposes in India. As the oil of the plant is extensively used in number of herbal preparation for curing inflammatory disorders, the present study was undertaken to assess analgesic and anti-inflammatory activities of its bark extract. Dried and crushed leaves ofPinus roxburghiiSarg. were defatted with petroleum ether and then extracted with alcohol. The alcoholic extract at the doses of 100 mg/kg, 300 mg/kg, and 500 mg/kg body weight was subjected to evaluation of analgesic and anti-inflammatory activities in experimental animal models. Analgesic activity was evaluated by acetic acid-induced writhing and tail immersion tests in Swiss albino mice; acute and chronic anti-inflammatory activity was evaluated by carrageenan-induced paw oedema and cotton pellet granuloma in Wistar albino rats. Diclofenac sodium and indomethacin were employed as reference drugs for analgesic and anti-inflammatory studies, respectively. In the present study, the alcoholic bark extract ofPinus roxburghiiSarg. demonstrated significant analgesic and anti-inflammatory activities in the tested models.

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AMLODIPINE-INDUCED TESTICULAR DNA DAMAGE AND ITS MODIFICATION BY A HERBAL PREPARATION IN WISTAR ALBINO RATS

INDIAN DRUGS ◽

10.53879/id.58.06.11610 ◽

2021 ◽

Vol 58 (06) ◽

pp. 42-48

Author(s):

Shini Dominic ◽

◽

Padmaja V.

Keyword(s):

Dna Damage ◽

Calcium Channel ◽

Calcium Channel Blockers ◽

Reproductive Potential ◽

Repeated Administration ◽

Albino Rats ◽

Channel Blockers ◽

Protective Agent ◽

Alcoholic Extract ◽

Wistar Albino Rats

The present study originated from the reports of adverse effects on male reproductive potential caused by the dihydropyridine derivative type calcium channel blockers, which are widely used agents for different cardiovascular disorders. The study was conducted to verify whether the repeated administration of this type of calcium channel blockers can cause any testicular DNA damage and if so, whether the damage can be modified by the co-administration of a herbal protective agent. The study was conducted in male Wistar albino rats. The testicular DNA damage of different groups of rats was evaluated by comet assay taking percentage DNA in comet tail as the main parameter. It was found that oral administration of the widely used dihydropyridine type calcium channel blocker amlodipine produced a duration- dependent and significant (P<0.001) DNA damage in the testes of Wistar albino rats. The co- administration of the alcoholic extract of seeds of Asteracantha longifolia produced a dose dependent and significant ((P<0.001) protective effect against this amlodipine-induced testicular DNA damage.

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