scholarly journals Multi-layer Budding of Oversized Rubber (Hevea brasiliences) Seedlings: Success and Growth in Different Soil Media

Author(s):  
Corpuz, Onofre S. ◽  
K. U. Guiamal

The study on Multi-layer budding of oversized rubber seedling aimed at evaluating the success of multiple budding operation on single seedling of rubber tree species comprising of 4 to 7 budding layers per seedling. The experiment used randomized complete blocked design layout with 3 treatments such as 4, 5 and 7 layer budding in a single seedling replicated 4 times. Results revealed that the highest number of budding layers succeeded significantly higher as compared to 4 and 5 layers. The main survival percentage is about 79.17% for the 7 layers, 76.52% for the 5 layers and only about 55.36% for the 4 layers. Pure garden soil reported to have significant results on sprouting rate, sprout length, stalk diameter and number of leaves. This finding emphasized that budding of rejected oversized rubber seedlings grown in an abandoned nurseries can be reutilized for mass production of budded cuttings through multiple budding of 7 layers per seedlings to maximize planting materials derived from single seedlings.

2016 ◽  
Vol 8 (3) ◽  
pp. 1235-1240
Author(s):  
P. A. Sofi ◽  
Sajad A. Bhat ◽  
T. H. Masoodi ◽  
M. A. Islam ◽  
G. M. Bhat ◽  
...  

Propagation of Himalayan maple (Acer caesium Wall.) through seed and softwood cuttings was investigated to standardize nursery techniques for mass production of the species. The seedlings were raised from viable seeds in different containers filled with different combinations of growing media. Vegetative propagation of softwoodcuttings was carried out by treating with different combinations of IBA and Willow leachate of different durations. The statistical analysis revealed the differential behaviour of various nursery stocks with respect to survival, growth and biomass. The growing media soil:sand:vermicompost (2:1:2) and container (root trainer 300 cc) showed maximum germination (61.00%), seedlings height (35.17 cm), collar diameter (5.07 cm), shoot:root ratio (1.24) and survival percentage (76.33%). Conversely, the cuttings treated with IBA @ 8000 ppm showed maximum sprouting (74.50%), rooting (66.75%), length of longest root (14.65 cm), no. of roots per cutting (33.00%), shoot length (13.90 cm) and survival percentage (41.50%). Hence, the seeds of the species should be grown in Rot trainer of 300 cc having soil:sand:vermicompost (2:1:2) to get good quality planting materials. However, the softwood cuttings should be given treatments with IBA @ 8000 ppm for mass production of plants vegetatively.


2020 ◽  
Vol 34 (03) ◽  
Author(s):  
Niharika Rathore ◽  
R. Lekhi ◽  
Arjun Kashyap ◽  
P. K.S. Gurjar

An experiment was conducted during 2017-18 at the Research orchard of Horticulture, college of Agriculture Gwalior to Study of interaction Effect of different colour poly wrappers and PBZ concentration on rooting and growth of Guava air layers. Total 12 treatment were tested under experiment. Result related that maximum length of primary root, length of secondary root, diameters of primary root, diameters of secondary root, fresh weight of root, dry weight of root, rooting %, number of leaves, number of new sprout, length of air layers, and survival percentage of air layers of guava was observed under Red poly wrapper with 500ppm PBZ. Which was significantly higher then other treatment combination follow by Blue poly wrapper with 500ppm PBZ and result of black poly wrappers and the lowest result found in white wrapper with co (No harmon) PBZ.


2019 ◽  
Vol 2 (1) ◽  
pp. 180-192
Author(s):  
Divya Pandey ◽  
Bishal Shrestha ◽  
Madan Sapkota ◽  
Sarita Banjade

This study was conducted at Boch, Bhimeshwor-8, Dolakha, Nepal from January to May, 2019 to study the effect of scion variety and wrapping materials on growth performance of kiwi seedling rootstock. The field experiment was carried out in Factorial Randomized Complete Block Design using four replications. The treatments consisted of four scion varieties (Monty, Bruno, Hayward, Allison) grafted onto one year old kiwi seedling (Actinidia deliciosa) and two types of wrapping material (Grafting tape and Polyethylene plastic). The measured traits included sprout length, diameter, number of leaves, and number of sprouted bud per graft, graft success, mortality and survival percentage of grafts. The success rate of kiwi grafting was significantly affected by the scion variety and the wrapping materials. Allison variety showed the minimum days (61.72 days) to first sprouting and the maximum length of sprouts, diameter, number of leaves and number of sprouted bud per graft at the final observation. Monty variety showed the lowest growth performance. The maximum graft success (96.87%) and survival percentage of grafts (93.75%) was observed in Allison variety statistically at par with Bruno and Hayward and the lowest graft success(73.44%) and survivability(64.21%) was observed in Monty due to high mortality of the sprouted grafts. Grafting tape was superior to polyethylene plastic in terms of days to first sprouting (64.08 days), number of sprouted buds per grafts, number of leaves, graft success (92.18%) and survival of the grafts (87.01%) at the final observation. Interactive effect was found non-significant. In a nutshell, Allison is the best scion variety for grafting under the climatic condition of Dolakha and the grafting tape was the suitable tying material.


Author(s):  
Nora Augustien ◽  
Pawana Nur Indah ◽  
Purnawati Arika ◽  
Irsyad Irsyad ◽  
Hadi Suhardjono

Indian mustard plants are the main vegetable crops consumed by urban communities. The need for this vegetable increases along with the increase in culinary tourism in each region and the government's appeal on sustainable food home. One effort made to meet the needs of vegetable mustard in urban areas can be cultivated using polybags. The main problem of vegetable cultivation on polybags is the availability of soil media. To overcome the limitations of soil media it is attempted to substitute it with organic litter enriched with MOL (local microorganisms). The purpose of research is to find the right combination of planting media for mustard plants. The results showed that the composition of K7 = soil: compost: litter of hay: litter of maize (2: 1: 1: 1) best on plant length, number of leaves, length of root leaves and number of roots while the wet weight of mustard plant (g) K7 = soil: compost: litter of maize: litter of corn (2: 1: 1: 1) and K8 = soil: compost: litter of maize: litter of maize: cocopeat (2: 1: 1: 1: 1) or soil: compost + urea. Increased weights of wet mustard by 63% compared to soil media and 20% compared to soil composition: compost + urea. Organic straw in the form of straw: litter of corn stalk: cocopeat enriched with MOL are able to become ready-made planting media on the cultivation of indian mustard plants in polybags.


2015 ◽  
Vol 77 (24) ◽  
Author(s):  
Siti Suhaila A. Rahman ◽  
Norwati Muhammad ◽  
Nor Hasnida Hassan ◽  
Haliza Ismail ◽  
Nazirah Abdullah ◽  
...  

Neolamarckia cadamba (kelempayan) is a multipurpose and fast growing timber species. The tree is grown for timber, paper-making and as ornamental plant. It is reported that its barks and leaves possesed medicinal values and its flowers are used in perfumes. The species is also known to be suitable for plywood, packing case, toys and short-fibred pulp. Therefore, mass production of high quality planting material of N. cadamba is important to support plantation program of this species. Here we presented mass production of N. cadamba through tissue culture techniques. Nodal segments derived from in vitro germinated seeds were used and induced direct organogenesis to produce shoots and roots using MS media (1962) and plant growth regulators (BAP and IBA) that are relatively cheaper than previously used methods. The tissue culture technique of N. cadamba developed may help in ensuring supply of planting materials that are feasible for commercial plantation purposes.


2021 ◽  
Vol 4 (2) ◽  
pp. 122-129
Author(s):  
Enda Sabda Gentri Sembiring ◽  
Julaili Irni ◽  
Rama Riana Sitinjak ◽  
Bayu Pratomo

Introduction: This study aims to determine the growth response of Mucuna bracteata cuttings to the concentration and duration of soaking shallot extract. Materials and Methods: This study used a factorial Randomized Block Design (RBD) with two factors, namely: shallot extract concentration (P) consists of 4 levels, include P0= untreated, P1= 10 ml, P2= 20 ml, P3= 30 ml, meanwhile the soaking time (Q) consists of 4 levels, such as Q0= momentarily dipping, Q1= 15 minutes, Q2= 30 minutes, Q3= 45 minutes. The data was processed by ANOVA and if significant, then further tested with Duncan Multiple Range Test (DMRT) at the rate of 5%. Results: The concentration of shallot extract, soaking time and their interaction had a very significant effect on the survival percentage of Mucuna bracteata D.C. cuttings. The highest survival percentage of cuttings of Mucuna bracteata on the effect of shallot extract concentration and soaking time was obtained in the P0Q0, P0Q1, and P3Q2 treatments after the lid was opened at 4 weeks after planting. In the number of leaves also had significant effect in the interaction of two factors


2015 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Erna Sinaga ◽  
Megayani Sri Rahayu ◽  
Awang Maharijaya

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>


2009 ◽  
Vol 2009 ◽  
pp. 1-12 ◽  
Author(s):  
Shigeki Hirobayashi ◽  
Yusuke Tamura ◽  
Kazuhiro Yamamoto

Some animals and plants function as bioantennas in that changes in their surrounding environment produce variations in their bioelectric potentials. While the bioelectric potential is affected by living activities of the plant, it has been observed that the bioelectric potential can be reduced using plants. Thus, the influence of the life activity of a plant on the reception signal must be accounted for when a plant is used as a sensor. In this study, we produced an environmental change near a foliage plant growing in an indoor environment and examined the directivity of the plant's sensing ability. The sensitivity of the plant was a roughly circular area centered on the location of the plant. We also investigated the influence of the number of leaves on the plant on its sensing ability and found that it decreased with a reduction in the number of leaves. In addition, we monitored the effect of a person walking on the spot near the plant on the bioelectric potential of the plant. Six subjects stepped on the spot 50 cm from a rubber tree and we measured the variation in the bioelectric potential of the tree produced by this stepping motion. The results confirmed that stepping motion produces a measurable response in the bioelectric potential of a plant and that this response varies in synchrony with the subject's stepping rate. Moreover, by conducting principal component analysis using the peak value of the spectrum characteristics of the measured bioelectric potential, cumulative proportion was found to reach nearly 97% at low-frequency components up to the fifth peak.


2020 ◽  
Vol 12 (5) ◽  
pp. 48
Author(s):  
Paulo César Ferreira Linhares ◽  
Roberto Pequeno de Sousa ◽  
Janilson Pinheiro de Assis ◽  
Eudes de Almeida Cardoso ◽  
Joaquim Odilon Pereira ◽  
...  

The use of plant resources available on the farm, and of great relevance to the family farmers of the Northeastern semi-arid region, Brazil. The experiment was carried in the experimental area of the agricultural science center, Universidade Federal Rural do Semi-&aacute;rido (UFERSA), Mossor&oacute;, Brazil, with the objective of evaluating use of Senna uniflora as organic fertilizer in the production of lettuce in the Brazilian semiarid, from October 2014 to February 2015. The experimental design of randomized complete blocks with the treatments arranged in 4 &times; 4 factorial scheme, with three replicates. The first factor consisted of amounts S. uniflora (0, 1.8, 3.6, and 5.4 kg m-2 of dry matter) with four incorporation times into the soil (0; 28; 56 and 84 days before transplanting lettuce). The transplanted lettuce cultivar went was the &ldquo;Elba&rdquo;. The evaluated characteristics were the following: plant height, diameter plant, number of leaves per plant, green mass production and dry mass production. The best agronomic efficiency was obtained with soil incorporation of 5.4 kg m-2 in the incorporation period of 56 days after transplanting, with phytomass production of 235.2 g plant-1. S. uniflora becomes a viable option to be used as an organic fertilizer in lettuce production.


2017 ◽  
Vol 23 (3) ◽  
pp. 257
Author(s):  
Ronan Carlos Colombo ◽  
Vanessa Favetta ◽  
Maria Aparecida da Cruz ◽  
Deived Uilian de Carvalho ◽  
Sergio Ruffo Roberto ◽  
...  

The in vitro propagation techniques are commonly used to produce ornamental pineapple seedlings in commercial scale, aiming to attend the growers with genetic and sanitary quality seedlings. However, the choice of the ideal substrate is essential for the acclimatization and growth stage of the seedlings propagated by this technique, since some substrates can increase the seedling mortality and/or limit the seedling growth due to its physical and chemical characteristics. Thus, the aim of this study was to evaluate the acclimatization of ornamental pineapple [Ananas comosus (L.) Merr. var. ananassoides (Baker) Coppens & Leal] on different substrates. Seedlings with approximately seven centimeters, obtained from in vitro culture, were transplanted into styrofoam trays filled with the following substrates: sphagnum; semi-composed pine bark; carbonized rice husk; sphagnum + semicomposed pine bark; sphagnum + carbonized rice husk; and semi-composed pine bark + carbonized rice husk. Each treatment was replicated five times using 10 plants. At 180 days, there were evaluated the following variables: survival percentage, plant height, number of leaves, leaf area, largest root length, and shoot and root dry matter. The substrate semi-composed pine bark + carbonized rice husk presented the lowest mean (62%) for survival percentage. The semi-composed pine bark and semi-composed pine bark + carbonized rice husk treatments presented significant increments in some evaluated biometric characteristics. The semi-composed pine bark is the most favorable substrate for the A. comosus var. ananassoids acclimatization.


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