scholarly journals Synthesis Spectroscopy and Computational Studies of Manganese (II) Complexes Incorporating (N,O)(O,O`) Ligands and Their Biological Activity

2019 ◽  
pp. 1-10
Author(s):  
Rania Ahmad Abokhater ◽  
Salem Eltuhami Ashoor ◽  
Suad A. Gadir
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Optimization Method ◽  
Computational Studies ◽  
Tandem Mass ◽  
Convergence Criteria ◽  
Human Pathogens ◽  
Klebsiella Spp ◽  
Chloride Hydrate

Manganese (II) complexes were synthesized via the reaction equimolar quantity of Manganese(II) chloride-hydrate (MnCl2.6H2O) with Chiral saccharides such {(OH)5(CH2)(C5H5)O} or {(OH)6(CH2)(C5H5)O} and (C9H7NO) as secondary ligands [Mn(C9H7NO){(OH)5(CH2)(C5H5)O}]Cl2 (C1), [Mn(QH){(OH)6(CH2)(C5H5)O}]Cl2 (C2), respectively where (C9H7NO) is 8-hydroxyquinoline, {(OH)5(CH2)(C5H5)O} Dextrose (Dex), {(OH)6(CH2)(C5H5)O} fructose (fru). Theses complexes were characterized by UV-vis, IR spectroscopy, tandem mass spectrometry and elemental analysis. The complexes have been found to have square planer geometry as the optimization method. The molecular geometries obtained from XRD data. The pre-optimized to standard convergence criteria using the basis Minimize Energy to Minimum. The isolated compounds were screened for its antibacterial activity against six standard human pathogens (Staphylococcus aureus, Streptococcus spp., Escherichia coli, Klebsiella spp., Psuedomones spp. and Protues spp.) and the results were obtained. About 10 to 70% for complex (C1) and about 10 to 60% for complex (C2) of the activity.

scholarly journals Top-Down Proteomic Identification of Shiga Toxin 1 and 2 from Pathogenic Escherichia coli Using MALDI-TOF-TOF Tandem Mass Spectrometry

2019 ◽  
Vol 7 (11) ◽  
pp. 488 ◽  
Author(s):  
Clifton K. Fagerquist ◽  
William J. Zaragoza ◽  
Michelle Q. Carter
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Tandem Mass Spectrometry ◽  
Disulfide Bond ◽  
Shiga Toxin ◽  
Tandem Mass ◽  
Top Down ◽  
E Coli ◽  
B Subunit ◽  
Proteomic Identification

Shiga-toxin-producing Escherichia coli (STEC) are a burden on agriculture and a threat to public health. Rapid methods are needed to identify STEC strains and characterize the Shiga toxin (Stx) they produce. We analyzed three STEC strains for Stx expression, using antibiotic induction, matrix-assisted laser desorption/ionization time-of-flight-time-of-flight (MALDI-TOF-TOF) mass spectrometry, and top-down proteomic analysis. E. coli O157:H- strain 493/89 is a clinical isolate linked to an outbreak of hemolytic uremic syndrome (HUS) in Germany in the late 1980s. E. coli O145:H28 strains RM12367-C1 and RM14496-C1 were isolated from an agricultural region in California. The stx operon of the two environmental strains were determined by whole genome sequencing (WGS). STEC strain 493/89 expressed Shiga toxin 2a (Stx2a) as identified by tandem mass spectrometry (MS/MS) of its B-subunit that allowed identification of the type and subtype of the toxin. RM12367-C1 also expressed Stx2a as identified by its B-subunit. RM14496-C1 expressed Shiga toxin 1a (Stx1a) as identified from its B-subunit. The B-subunits of Stx1 and Stx2 both have an intramolecular disulfide bond. MS/MS was obtained on both the disulfide-bond-intact and disulfide-bond-reduced B-subunit, with the latter being used for top-down proteomic identification. Top-down proteomic analysis was consistent with WGS.

scholarly journals ANTIMICROBIAL POTENTIALS OF SOIL FUNGI METABOLITES, MOLECULAR DYNAMICS AND THEIR 3D PROTEIN STRUCTURAL PREDICTION USING BIOINFORMATICS TOOL

2020 ◽  
pp. 20-26
Author(s):  
MAJOLAGBE O. N. ◽  
AINA D. A. ◽  
OMOMOWO I. O. ◽  
THOMAS A.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Protein Structure ◽  
Secondary Metabolites ◽  
Soil Fungi ◽  
3D Structure ◽  
Antimicrobial Activities ◽  
Biological Properties ◽  
3D Protein Structure ◽  
Klebsiella Spp

Objective: To determine the antimicrobial potentials of secondary metabolite of soil fungi and predict their 3D structure and molecular identity. Methods: Pure soil fungi were isolated from soil samples and cultured under submerged fermentation (Smf) for their metabolites using Potato Dextrose Agar and Broth. The secondary metabolites of the isolated fungi were obtained intracellularly after 21 d of incubation in a rotary shaker incubator. The antimicrobial potentials of the metabolites were investigated against four (4) clinical isolates, namely: Staphylococcus aureus, Klebsiella spp, Candida albicans and Escherichia coli. These soil fungi were further characterized to the molecular level and their evolutionary relationships established using bioinformatics tools. Protein structure of each of the fungi isolates was predicted using PHYRE-2. Results: Out of all the soil fungi isolated, the metabolite of Aspergillus aculeatus showed the highest antimicrobial activities against Staphylococcus aureus (23.00±2.34 mm), Escherichia coli (9.00±1.44 mm) and Klebsiella spp (24.00±3.45 mm). The 3D protein structure predicted showed that each of the organisms consists of different amino-acid compositions such as: serine, tyrosine, proline, arginine, glycine, phenylalanine leucine with other notable biological properties. Conclusion: The work revealed that secondary metabolites of the isolated fungi carry an important role in combating infectious agents thereby, providing roadmaps for the biosynthesis of many synthetic and semi-synthetic drugs and bio-products which are environmentally friendly.

scholarly journals Antioxidant and Antibacterial Activities of the Essential Oil of Moroccan Tetraclinis articulata (Vahl) Masters

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Halima Rabib ◽  
Chaimaa Elagdi ◽  
Mohammed Hsaine ◽  
Hassan Fougrach ◽  
Tayeb Koussa ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antioxidant Properties ◽  
Antibacterial Activities ◽  
Bornyl Acetate ◽  
Radical Trap ◽  
Tetraclinis Articulata

The purpose of this study is to evaluate and compare the antioxidant and antibacterial activities of essential oil isolated from Tetraclinis articulata (Vahl) leaves, Masters originating in Morocco (Benslimane Region, Atlantic-influenced plain). The analysis of the major compounds of essential oil was performed by gas chromatography and mass spectrometry, and this oil is dominated by bornyl acetate (35.05%), camphor (11.17%), and α-pinene (10.84%). The antioxidant properties were evaluated by the test of the radical trap 2,2-diphényl-1-picrylhydrazyl (DPPH), and the antimicrobial activity of T. articulata essential oil was tested against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli which have been inhibited from the 25 μg/mL.

Sign in / Sign up

Export Citation Format

Share Document