Effect of Black Seed (Nigella sativa) and Uziza (Piper guineense) Leaf on the Histology of the Liver of Wistar Albino Rats

Aim: This study was aimed at investigating the effects of aqueous extracts of both Nigella sativa and Piper guineense on the liver enzymes; alanine amino transferase (ALT), aspartate amino transferase (AST) and alkaline phosphatase (ALP). Also the effect of Nigella sativa and Piper guineense extracts on the histology of the liver of Wistar rat was also studied. Materials and Methods: A total of twenty five Wistar rats were used for the study. The animals were grouped into five groups, each having five animals. They were induced with sucrose and margarine to cause high sugar levels and hyperlipidemia respectively except the positive control group which was fed normal feed. The groups were: the positive control group, the negative control group which were induced without treatment, the uziza leaf group which were induced and were treated with 2 ml of aqueous extract of uziza leaf, the black seed group which were induced and were treated with 2 ml of aqueous extract of black seed, and the black seed and uziza group which were induced and were treated with 2ml of aqueous extract of black seed and 2 ml of aqueous extract of uziza leaf. Results: The result showed that the extracts decreased the ALT and AST and ALP activities in the rats in a time dependent manner with highest decrease obtained on the third week of treatment with the extracts. The ALT activity (U/L) on the third week of treatment showed for the, negative control (64.48 ± 0.22), uziza leaf (28.82 ± 0.12), black seed (32.65 ± 0.02), black seed and uziza leaf (16.04 ± 0.02) (p≤0.05). The decrease in activity for AST levels (U/L) on the third week of treatment, showed for the negative control (58.00 ± 0.02), uziza leaf (11.00 ± 0.01), black seed (12.00 ± 0.02), black seed and uziza leaf (8.00 ± 0.02). Conclusion: It can be concluded that both uziza leaf and black seed have hepatoprotective effect on the liver.

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Effects of Black Seed (Nigella sativa) and Uziza Leaf (Piper guineense) on Electrolytes, Urea and Creatinine of Wistar Albino Rats

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2019/v26i230092 ◽

2019 ◽

pp. 1-10

Author(s):

Okoye Ngozi Franca ◽

Igwilo, Elvis Ikenna

Keyword(s):

Aqueous Extract ◽

Wistar Rats ◽

Nigella Sativa ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Albino Rats ◽

Piper Guineense ◽

The Third ◽

Black Seed

Aim: For centuries, plant and plant products have played a pivotal role in medication. This study evaluated the effect of aqueous extract of black seed (Nigella sativa) and uziza leaf (Piper guineense) on electrolytes, urea and creatinine of Wistar rats. Materials and Methods: Twenty-five Wistar rats were used for the study; the rats were arranged into five groups with five rats each. Sucrose and margarine were used to induce hyperglycemia and hyperlipidemia respectively on the rats with the exception of the rats in the positive control group. The rats in the negative control were induced using the sucrose and margarine but were not treated using the aqueous extracts. The rats in the uziza group were treated with 2 ml of uziza aqueous leaf extract, while the rats in the black seed group were treated with 2 ml of black seed aqueous extract. The rats in the black seed & uziza leaf group were treated with 2ml of the combined aqueous extract. Results: The results showed that the extracts had a decreasing effect which was time dependent on the electrolytes. The highest decrease was obtained on the third week of feeding compared to the control (P=.05). The sodium levels (mmol/L) showed for the negative control (198.23 ± 1.96), positive control (108.15 ± 1.60), uziza leaf (98.28 ± 4.17), black seed (101.67 ± 4.24), black seed & uziza (90.83 ± 2.14). The decrease for potassium levels (mEq/L) showed for the negative control (0.90 ± 0.06), positive control (0.05 ± 0.10), uziza leaf (0.07 ± 0.18), black seed (0.06 ± 0.19), black seed & Uziza (0.05 ± 0.10). Furthermore, the extracts also had a reducing effect on urea and creatinine levels with the highest reduction obtained on the third week (p=0.05). The urea levels (mmol/L) showed for the negative control (26.84 ± 0.05), positive control (15.15 ± 1.20), uziza leaf (12.83 ± 0.98), black seed (12.16 ± 2.01), black seed & uziza (11.48 ± 1.78). The extracts also decreased creatinine levels (mmol/L) with the negative control (284.58 ± 0.33), positive control (182.73 ± 3.67), uziza leaf (194.16 ± 18.30), black seed (167.34 ± 14.66), black seed & uziza (174.46 ± 10.66). Conclusion: The extracts significantly decreased the elevated electrolytes levels and therefore uziza leaf and black seed can be used to restore kidney function.

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The Effect of Nigella sativa and Piper guineense Aqueous Extracts on the in Vivo Expression Profile of Lipid and Sex Hormones

Biotechnology Journal International ◽

10.9734/bji/2019/v23i130069 ◽

2019 ◽

pp. 1-14

Author(s):

Okoye Ngozi Franca ◽

Uvbiekpahor Rume

Keyword(s):

Aqueous Extract ◽

Nigella Sativa ◽

Follicle Stimulating Hormone ◽

Positive Control ◽

Aqueous Extracts ◽

Piper Guineense ◽

Male And Female ◽

Hormone Levels ◽

The Third ◽

Black Seed

Aim: The study investigated the effect of aqueous extract of Piper guineense and Nigella sativa on Testosterone, Estrogen and Follicle stimulating hormone levels in male and female rats induced by diets supplemented with high dose of sucrose and margarine for four (4) week. Materials and Methods: Thirty male and female Wistar rats of average weight (100 g) were used for the study. The rats were arranged into five groups with six rats in each of the groups. The rats had access to their normal feed but sucrose and margarine were used to induce hyperglycemia and hyperlipidemia respectively on the rats with the exception of the rats in the positive control group. The rats in the negative control were induced using the sucrose and margarine but were not treated using the aqueous extracts. The rats in the Piper guineense group were treated with 2 ml of Piper guineense aqueous leaf extract, while the rats in the Nigella sativa group were treated with 2 ml of Nigella sativa aqueous extract. The rats in the Nigella sativa and Piper guineense group were treated with 2 ml of the combined aqueous extract. Results: The results showed that the extracts had an increasing effect which was time dependent on the hormones. The highest increase was obtained on the third week of feeding when compared to the control. The testosterone levels (mlU/ml) showed for the, positive control (2.28 ± 0.08), uziza leaf (6.45 ± 0.01), black seed (2.85 ± 0.01), black seed &uziza (8.47 ± 0.08)(p<0.05). The increase for estrogen levels (mlU/ml) showed for the positive control (99 ± 0.82), uziza leaf (98 ± 1.63), black seed (100 ± 1.63), black seed & uziza (113 ± 0.82). Furthermore, the extracts also had an increasing effect on the follicle stimulating hormone levels with the highest increase obtained on the third week (p>0.05). The FSH levels (mlU/ml)) showed for the positive control (0.23 ± 0.01), uziza leaf 0.25 ± 0.01), black seed (0.54 ± 0.02), black seed & uziza (0.58 ± 0.01)(p<0.05). Histopathological findings also suggest that treatment with the aqueous extracts of P.guineense and N .sativa after inducement salvaged the testes and ovaries when compared to the induced group after the second week of administration. Although the combined mixture N.sativa and P.guineense extract was more effective. Conclusion: The results of the study indicate that the aqueous extract of a combined mixture of Nigella sativa and Piper guineense might be an effective plant in indigenous medicine, which may be used to combat infertility caused by hormonal imbalance.

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PENGARUH PEMBERIAN EFEK JINTEN HITAM (NIGELLA SATIVA) TERHADAP APOPTOSIS TERHADAP SEL EPITEKL BRONKIOLUS DAN APOPTOSIS LIMFOSIT T SALURAN NAFAS BRONCHIOLUS PADA MODEL MENCIT ASTHMA

Saintika Medika ◽

10.22219/sm.v6i2.1060 ◽

2012 ◽

Vol 6 (2) ◽

Author(s):

Endang Sriwahyuni ◽

IMAMA KHALIS NUR ARIFAH

Keyword(s):

Mouse Model ◽

Bronchial Asthma ◽

Nigella Sativa ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Seed Extract ◽

Positive Control Group ◽

Control Group ◽

Black Seed

Abstract Airway remodelling refers to the structural changes that occur in the airway wall in asthma. These is irreversible changes to the airway, with damage of bronchiolus epithelium because the airway epithelium undergoes apoptosis as one of its manifestations. Empirically black seed has been known as bronchial asthma therapy for many years ago. The purpose of the research is to test the effect of black seed (Nigella sativa L.) extract to apoptosis of bronchiolus epithelium and apoptosis of T lymphocytes the airway bronchial asthma on asthma mouse model. Female mice were used in the expeimental laboratory research and allergic mouse model was got by given ovalbumin twice within 3 weeks intraperitoneally and 3 times per week within 6 weeks by inhalation. Black seed extract is given in 3 different dose (0,024 cc/day, 0,048 cc/day and 0,096 cc/day) for 9 weeks. Sample was chosen randomly to share it within 5 group containing 6 mice in every group ; negative control group (without any treatment, n=6), positive control group (sensitizied with ovalbumin, n=6), JH1 group (sensitizied with ovalbumin and treating with first dose of black seed extract, n=6), JH2 group (sensitizied with ovalbumin and treating with second dose of black seed extract, n=6), JH3 group (sensitizied with ovalbumin and treating with third dose of black seed extract, n=6). The parametric that is used in this research is the change in the color of the nucleus of bronchiolus epithelium and T lymphocytes the airway bronchial asthma shown the occurrence of browning apoptosis. The result shown a significant increase of apoptosis of bronchiolus epithelium on positive control group to compare with negative control group. The apoptosis of bronchiolus epithelium on JH1 shown an increase compare with positive control group. For JH2 and JH3 groups shown a decrease the apoptosis of bronchial epithelium compare with positive control group. And the research indicate that the unsignificant aptosis decrease were happen on a group with positive control instead of the group with negative control. Meanwhile, on JH1 group, apoptosis decrease were happening more significantly than the positive control group does, the aptosis increases were happen on JH2 and JH3 group, but its not capable to exceeding the positive control group apoptosis amount The conclusions is the black seed (Nigella sativa L.) extract can decrease apoptosis of bronchiolus epthelium on asthma mouse model in the third dose as the best result and the doses of black seed extract that were giving to the experiment mice can not increasing the apoptosis T Lymphocyte, thus requiring further research to figure out the perfect doses to increasing against respiratory tract apoptosis T Lymphocyte. Keyword: asthma, black seed, the apoptosis of bronchiolus epithelium, apoptosis limfosit T.

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Effects of Aqueous Extracts of Nigella sativa and Ocimum gratissimum on Electrolyte, Urea, Creatinine of Wistar Rat

Journal of Applied Life Sciences International ◽

10.9734/jalsi/2019/v21i330107 ◽

2019 ◽

pp. 1-8

Author(s):

Okoye Ngozi Franca ◽

Benjamin Prince

Keyword(s):

Aqueous Extract ◽

Wistar Rats ◽

Nigella Sativa ◽

Negative Control ◽

Time Dependent ◽

Dependent Manner ◽

Aqueous Extracts ◽

Ocimum Gratissimum ◽

The Third ◽

Black Seed

Aim: Effects of aqueous extracts of Nigella sativa (black seed) and Ocimum gratissimum (scent leaf) on electrolytes, urea and creatinine of Wistar rats were investigated. Materials and Method: Twenty-five Wistar rats were used for the study, the rats were arranged into five groups with five rats in each of the groups. The rats had access to their normal feed but sucrose and margarine were used to induce hyperglycemia and hyperlipidemia respectively on the rats with the exception of the rats in the positive control group. The rats in the negative control were induced using the sucrose and margarine but were not treated using the aqueous extracts. The rats in the scent leaf group were treated with 2 ml of scent leaf aqueous extract, while the rats in the black seed group were treated with 2 ml of black seed aqueous extract. The rats in the black seed and scent leaf group were treated with 2 ml of the combined aqueous extract. Results: The result showed that the extracts decreased the levels of the electrolytes in the rats in a time-dependent manner with the highest decrease obtained on the third week of treatment with the extracts. The sodium levels (mmol/ml) on the third week of treatment showed for the negative control (193.83 ± 2.96), scent leaf (125.80 ± 8.27), black seed (119.60 ± 6.24), black seed and scent leaf (110.93 ± 10.14) (p<0.05). The decrease for potassium levels (mEql/l) on the third week of treatment, showed for the negative control (0.11 ± 0.05), scent leaf (0.09 ± 0.14), black seed (0.08 ± 0.10), black seed and scent leaf (0.06 ± 0.11). Furthermore, the extracts also had a decreasing effect on the urea and creatinine in a time-dependent manner with the highest decrease obtained on the third week of treatment (p>0.05). The urea levels (mmol/ml)) showed for the negative control (26.64 ± 0.33), scent leaf (16.73 ± 0.88), black seed (15.86 ± 2.31) and black seed and scent leaf (12.88 ± 1.98). The decrease for creatinine levels (mmol/l) showed for negative control (662.68 ± 18.00), scent leaf (287.10 ± 12.30), black seed (192.44 ± 10.44) and black seed and scent leaf (188.66 ± 10.88. Conclusion: The extracts significantly decreased the elevated electrolytes, urea and creatinine levels and therefore scent leaf and black seed can be used to restore kidney function.

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Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

Jurnal Katalisator ◽

10.22216/jk.v3i1.2178 ◽

2018 ◽

Vol 3 (1) ◽

pp. 1

Author(s):

Verawaty Verawaty ◽

Dhea Claudia Novel

Keyword(s):

Blood Glucose ◽

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Male Mice ◽

Glucose Levels ◽

The Third ◽

Blood Glucose Levels

Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.

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Resistance to Fracture of Dental Roots Obturated with Different Materials

BioMed Research International ◽

10.1155/2015/591031 ◽

2015 ◽

Vol 2015 ◽

pp. 1-5 ◽

Cited By ~ 6

Author(s):

Berkan Celikten ◽

Ceren Feriha Uzuntas ◽

Kamran Gulsahi

Keyword(s):

Fracture Resistance ◽

Testing Machine ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Root Canal Filling ◽

Filling Materials ◽

Group 2

The aim of this study was to compare the vertical fracture resistance of roots obturated with different root canal filling materials and sealers. Crowns of 55 extracted mandibular premolar teeth were removed to provide root lengths of 13 mm. Five roots were saved as negative control group (canals unprepared and unfilled). Fifty root canals were instrumented and then five roots were saved as positive control group (canals prepared but unfilled). The remaining 45 roots were randomly divided into three experimental groups (n=15root/group) and obturated with the following procedures: in group 1, glass ionomer-based sealer and cone (ActiV GP obturation system); in group 2, bioceramic sealer and cone (EndoSequence BC obturation system); and in group 3, roots were filled with bioceramic sealer and cone (Smartpaste bio obturation system). All specimens were tested in a universal testing machine for measuring fracture resistance. For each root, the force at the time of fracture was recorded in Newtons. The statistical analysis was performed by using Kruskal-Wallis and post hoc test. There were no significant differences between the three experimental groups. The fracture values of three experimental and negative control groups were significantly higher than the positive control group. Within the limitations of this study, all materials increased the fracture resistance of instrumented roots.

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Biosafety devices to control the spread of potentially contaminated dispersion particles. New associated strategies for health environments

PLoS ONE ◽

10.1371/journal.pone.0255533 ◽

2021 ◽

Vol 16 (8) ◽

pp. e0255533

Author(s):

Victor Angelo Martins Montalli ◽

Patrícia Rejane de Freitas ◽

Milenna de Figueiredo Torres ◽

Oscar de Figueiredo Torres Junior ◽

Dienne Hellen Moutinho De Vilhena ◽

...

Keyword(s):

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

High Rate ◽

Positive Control Group ◽

Control Group ◽

Healthy Environment ◽

The Mean ◽

Petri Dishes ◽

Uv C

Dental procedures produce a large amount of spatter and aerosols that create concern for the transmission of airborne diseases, such as Covid-19. This study established a methodology with the objective of evaluating new associated strategies to reduce the risk of cross-transmission in a health environment by simulating spread of potentially contaminated dispersion particles (PCDP) in the environment. This crossover study, was conducted in a school clinic environment (4 clinics containing 12 dental chairs each). As a positive control group (without barriers), 12 professionals activated at the same time the turbine of dental drill, for one minute, with a bacterial solution (Lactobacillus casei Shirota, 1.5x108 CFU/mL), which had been added in the cooling reservoir of the dental equipment. In the experimental groups, the professionals made use of; a) an individual biosafety barrier in dentistry (IBBD) which consists of a metal support covered by a disposable PVC film barrier; b) a Mobile Unit of Disinfection by Ultraviolet-C, consisting of 8 UV lamps-C of 95W, of 304μW/cm2 of irradiance each, connected for 15 minutes (UV-C) and; c) the association between the two methods (IBBD + UV-C). In each clinic, 56 Petri dishes containing MRS agar were positioned on the lamps, benches and on the floor. In addition, plates were placed prior to each test (negative control group) and plates were also placed in the corridor that connects the four clinics. In the groups without barrier and IBBD + UV-C the passive air microorganisms in Petri dishes was also evaluated at times of 30, 60, 90 and 120 minutes after the end of the dental’s drill activation. The mean (standard deviation) of CFU of L. casei Shirota for the positive control group was 3905 (1521), while in the experimental groups the mean using the IBBD was 940 (466) CFU, establishing a reduction on average, of 75% (p<0.0001). For the UV-C group, the mean was 260 (309) CFU and the association of the use of IBBD + UV-C promoted an overall average count of 152 (257) CFU, establishing a reduction on average of 93% and 96%, respectively (p<0.0001). Considering these results and the study model used, the individual biosafety barrier associated with UV-C technology showed to be efficient strategies to reduce the dispersion of bioaerosols generated in an environment with high rate of PCDP generation and may be an alternative for the improvement of biosafety in different healthy environment.

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Efektivitas Ekstrak Artemisia vulgaris L. sebagai Hepatoprotektor pada sel-sel Hati Tikus yang Diinduksi Niasin

Pustaka Kesehatan ◽

10.19184/pk.v6i2.7617 ◽

2018 ◽

Vol 6 (2) ◽

pp. 251

Author(s):

Laily Rahmawati ◽

Erma Sulistyaningsih ◽

Rosita Dewi

Keyword(s):

Treatment Group ◽

Liver Damage ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Artemisia Vulgaris ◽

Group I ◽

Group Ii

The niacin in energy drinks has metabolic product that cause oxidative stress and liver damage, while the liver damage can be prevented by hepatoprotective agents. Scoparone in Artemisia vulgaris L. can act as a hepatoprotector by its antioxidant effect. This study aimed to investigate the effectivity of Artemisia vulgaris L. extract as a hepatoprotector in wistar hepatocytes induced by niacin. This study used 25 male rats which were divided into 5 groups: normal, the negative control, the positive control, the treatment group I, and II. Treatment was conducted for 28 days. The samples were terminated and the hepatocyte were prepared for histological examination. Histological appearance was catagorized as mild, moderate, and severe damage with or without inflamatory cells activity. The data analysis by Kruskal Wallis showed significant difference (p<0,001). Further analysis by Mann Whitney revealed significantly difference (p<0,05) between normal group and all groups, negative control group and positive control group, and positive control group and treatment group I, but not significantly difference between negative control group and treatment group I, negative control group and treatment group II, positive control group and treatment group II, and between treatment groups. The study concluded that the effectivity of Artemisia vulgaris L. extract has not been proven as a hepatoprotector but further study is needed to draw a definite conclusion. Keywords: energy drink, niacin, Artemisia vulgaris L., hepatoprotector

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PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

Biopendix: Jurnal Biologi, Pendidikan dan Terapan ◽

10.30598/biopendixvol1issue2page198-208 ◽

2015 ◽

Vol 1 (2) ◽

pp. 198-208

Author(s):

Nur Fitri

Keyword(s):

Treatment Group ◽

Rattus Norvegicus ◽

Leaf Extract ◽

Healing Process ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

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The Effect of Chemically Modified Tetracycline-3 on the Progression of Dental Caries in Rats

Caries Research ◽

10.1159/000481412 ◽

2018 ◽

Vol 52 (4) ◽

pp. 297-302 ◽

Cited By ~ 1

Author(s):

Jun Xu ◽

Congcong Miao ◽

Zhenchuan Tian ◽

Jinlu Li ◽

Chunmei Zhang ◽

...

Keyword(s):

Dental Caries ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Caries Prevalence ◽

Chemically Modified ◽

Dentin Caries ◽

Chemically Modified Tetracycline

Objective: Matrix metalloproteinases (MMPs) exist in human saliva and dentin and play an important role in the degradation of organic matrix in teeth. Chemically modified tetracycline-3 (CMT-3) is an inhibitor of MMPs. CMT-3 has been used experimentally to treat caries since 1999, but no distinction between dental caries prevalence and dentin caries prevalence has been described. Methods: A total of 65 Sprague-Dawley rats were randomly divided into three groups. The positive control group (25 rats) was inoculated with Streptococcus mutans (ATCC700610) and fed the cariogenic feed of improved Keyes Diet 2000. The CMT-3 group (25 rats) was also inoculated with S. mutans and fed the cariogenic feed of improved Keyes Diet 2000; the surfaces of rats’ molars were daily treated with 0.02% CMT-3. The negative control group (15 rats) was only fed the standard rodent chow. At the end of the 10th week, the dental caries prevalence and dentin caries prevalence of each group were calculated, and the regions of caries were assessed. Results: No caries was found in the negative control group. The dental caries prevalence of the CMT-3 and the positive control group was 75.0 and 83.3%, respectively (p > 0.05, Table 2). The dentin caries prevalence of the CMT-3 and the positive control group was 33.3 and 70.8%, respectively (p < 0.05, Table 2). The Keyes scoring of dentin caries in the CMT-3 group was significantly lower than that in the positive control group (p < 0.05, Table 3). Conclusions: CMT-3 had no effect on the prevalence of dental caries, but could lower the prevalence and slow down the progression of dentin caries.

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