Effect of Gibberellic Acid on Germination and Vigour of Kagzi Lime Seedlings

Aims: To investigate the effect of storage treatments, gibberellic acid (GA3) and duration of soaking on seed germination and seedling growth in Kagzi lime. Study Design: The experiment was laid out in a Completely Randomized Design (CRD) with factorial concept and three repetitions. Place and Duration of Study: The experiment was conducted during July to November 2017 at Department of Fruit Science, Navsari Agricultural University, Navsari, Gujarat, India. Methodology: The experiment consisted of sixteen treatments which were repeated thrice. It comprised of three factors and their respective levels. Factor S1 indicated freshly extracted kagzi lime seeds and S2 comprised of seeds stored for 15 days. The different concentrations of gibberellic acid were G1 - 200 ppm; G2 - 300 ppm; G3 - 400 ppm and G4 - 500 ppm. Whereas, duration D1 stood for 12 hours and D2 for 24 hours. Results: The interaction between storage treatments, pre-sowing treatments and duration of soaking was found significant for all parameters included in the study except collar diameter and sturdiness quotient. Soaking freshly extracted Kagzi lime seeds in an aqueous solution of 500 ppm GA3 for 12 hours resulted in the minimum days for 50% germination (27.89), Seedling Vigour Index (3654.83) and survival percentage (84.70). The same treatment when extended for 24 hours recorded the highest germination percentage (95.68) and number of leaves (28.76). Soaking freshly extracted seeds in 400 ppm GA3 solution for 12 hours registered the maximum shoot length (21.63 cm). Kagzi lime seedlings raised from freshly extracted seeds resulted in higher collar diameter (2.04 mm) and sturdiness quotient (8.70). Further, a soaking duration of 12 hours proved better over 24 hours for collar diameter (2.04 mm) and sturdiness quotient (8.69). Between the different concentrations of GA3, 400 ppm gave better results for collar diameter (2.09 mm) and 200 ppm for sturdiness quotient (8.65). Conclusion: Soaking freshly extracted kagzi lime seeds in an aqueous solution of 500 ppm GA3 for 12 hrs proved to be the best treatment combination for inducing early germination, higher Seedling Vigour Index and survival percentage. Nurserymen can employ these findings for early germination and vigorous seedlings in Kagzi lime.

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Effect of Pre-sowing Treatments on Vegetative Growth and Production of Graftable Rootstocks in Mango

International Journal of Bio-resource and Stress Management ◽

10.23910/1.2021.2209b ◽

2021 ◽

Vol 12 (6) ◽

pp. 655-662

Author(s):

Sanjeev Kumar Banyal ◽

◽

Shiv Kumar Shivandu ◽

Uday Raj Patial ◽

◽

...

Keyword(s):

Leaf Area ◽

Seed Coat ◽

Vegetative Growth ◽

Germination Percentage ◽

High Rate ◽

Science Department ◽

Seedling Vigour ◽

Intact Seed ◽

Vigour Index ◽

Number Of Leaves

The present investigations “Studies on the effect of pre-sowing treatment and time of wedge grafting on production of saleable grafts in mango” were carried out in the nursery block of Fruit Science Department. The study was conducted using wild mangoes seeds. The seeds were divided in two equal halves, one half of seeds were sown with intact seed coat (K1) while, in other half the mango kernels were extracted (K2), both type of seeds were then soaked in 6 different chemicals of varying concentrations viz. C1- KNO3 @ 0.5%, C2- KNO3 @ 1%, C3- KNO3 @ 1.5%, C4- BA @ 50 ppm, C5- BA @ 100 ppm, C6- GA3 @ 100 ppm, C7- GA3 @ 200 ppm, C8- beejaaumrit @ 2%, C9- beejaaumrit @ 3%, C10- panchgavya @ 2%, C11- panchgavya @ 3% for 12 hours and C12- Control. The germination and vegetative parameters were observed. The results of study revealed that the e×tracted kernels pre-soaked with GA3 @ 100 ppm produced earliest germination, 50 % germination, high rate of germination, germination percentage, number of leaves, maximum leaf area, highest seedling vigour index, while, on pre-soaking extracted kernel with KNO3 @ 0.5% produced maximum seedling diameter. The Seedling raised from extracted kernels pre-soaked in KNO3 @ 0.5% (C1K2) attained graftable diameter within 210 days of sowing. The present investigation was conducted during 2019-2020 at the nursery block of College of Horticulture and Forestry, Neri Hamirpur (H.P) to study the effect of pre-sowing treatments on germination, growth and production of graft-able seedlings.

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Influence of Gibberellic acid (GA3) on seed germination and seedling growth of Kagzi Lime

Journal of Scientific Agriculture ◽

10.25081/jsa.2017.v1.888 ◽

2018 ◽

Vol 1 ◽

pp. 62

Author(s):

W. S. Dilip, D. Singh, D. Moharana ◽

Sandeep Rout ◽

S. Patra

Keyword(s):

Seed Germination ◽

Gibberellic Acid ◽

Seedling Growth ◽

Dry Weight ◽

Germination Percentage ◽

Growth And Yield ◽

Similar Treatment ◽

Survival Percentage ◽

Tap Root ◽

Number Of Leaves

A study was conducted to estimate the influence of gibberellic acid (GA3) at different concentrations in different time intervals on seed germination and seedling growth of kagzi lime. The results revealed that Maximum germination percentage (95%) was recorded under treatment with GA3 80 ppm for 12 hours, rate of germination of seeds (25 days), height of plant (18.79 cm) at 120 DAS, number of leaves per plant (26.53), fresh and dry weight of shoot (25.84 g and 14.44 g), tap root (17.44 cm), secondary and fibrous roots (5.98 and 85.99), fresh as well as dry weight (7.04 and 4.95 g), survival percentage (85 percent) in similar treatment. Therefore, it may be concluded that the GA3 at 80 ppm has a significant effect on the seed germination and seedling growth of Kagzi Lime and can be recommended to the grower for obtaining better growth and yield.

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Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.6.1.20-28 ◽

2015 ◽

Vol 6 (1) ◽

pp. 20

Author(s):

Erna Sinaga ◽

Megayani Sri Rahayu ◽

Awang Maharijaya

Keyword(s):

Tissue Culture ◽

Drought Tolerance ◽

In Vitro Selection ◽

Solanum Melongena ◽

Survival Percentage ◽

Drought Tolerant ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design

ABSTRACTThe objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought tolerance of eggplant and the drought tolerant eggplant accessions. The experiment was conducted at the Laboratory of Tissue Culture, Department of Agronomy and Horticulture, Bogor Agricultural University. The experiment was arranged in a completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10, and 15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090). The results showed that the addition of PEG to in vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought ABSTRAKPenelitian ini bertujuan untuk mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG) terhadap pertumbuhan tanaman terung in vitro, mendapatkan konsentrasi PEG yang dapat digunakan untuk seleksi tanaman terung secara in vitro dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan, Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor. Penelitian ini disusun dalam rancangan acak lengkap dua faktor. Faktor pertama adalah konsentrasi PEG terdiri atas 0, 5, 10, dan 15%. Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090). Hasil penelitian menunjukkan bahwa penambahan PEG pada media in vitro memberikan pengaruh nyata dan sangat nyata terhadap persentase hidup eksplan, persentase eksplan berkalus, pertambahan tinggi tunas, dan jumlah daun tanaman terung. Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan

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Development of priming technology for enhanced planting value of seeds in kabuli chickpea (Cicer arietinum L.)

Journal of Applied and Natural Science ◽

10.31018/jans.v13i2.2507 ◽

2021 ◽

Vol 13 (2) ◽

pp. 735-743

Author(s):

Umesh Hiremath ◽

Basave Gowda ◽

Lokesh G.Y . ◽

B. S. Ganiger

Keyword(s):

Root Length ◽

Seed Quality ◽

Dry Weight ◽

Germination Percentage ◽

Quality Parameters ◽

Shoot Length ◽

Growth And Yield ◽

Seedling Vigour ◽

Seedling Length ◽

Vigour Index

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.

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Trichoderma Contributes to the Germination and Seedling Development of Açaí Palm

Agriculture ◽

10.3390/agriculture10100456 ◽

2020 ◽

Vol 10 (10) ◽

pp. 456

Author(s):

Bruno Fróes Campos ◽

Anselmo Junior Corrêa Araújo ◽

Cristina Aledi Felsemburgh ◽

Thiago Almeida Vieira ◽

Denise Castro Lustosa

Keyword(s):

Dry Mass ◽

Initial Growth ◽

Randomized Design ◽

Trichoderma Isolate ◽

Number Of Leaves ◽

Collar Diameter ◽

Completely Randomized Design ◽

Set Up ◽

Root Size ◽

Trichoderma Isolates

The application of Trichoderma fungus can be used to obtain seedlings of higher quality for plantations. The objective of this study was to evaluate the effects of Trichoderma isolates on the germination and initial growth of açaí seedlings. Two trials were set up. In the laboratory, the seeds were inoculated in suspensions prepared with each of the isolates for 24 h, and they were arranged in trays containing filter paper in a completely randomized design with five replicates containing 50 seeds each. For the control, the seeds were submerged only in water. Part of the seedlings resulting from seeds treated and not treated with Trichoderma were transferred to bags containing forest soil and were submitted to seven modes of application of Trichoderma and kept in a nursery. The speed of germination and hypocotyl were not influenced by the biological treatment. The application of the different Trichoderma isolates positively influenced the height, collar diameter, number of leaves, root size, leaf area and root, and aerial part dry mass of the açaí seedlings. The application of the Trichoderma isolate TAM01 on the preplanting substrate and biweekly applications on the postplanting substrate was the combination that most positively influenced the development of açai seedlings.

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CHARACTERIZATION OF SEEDS, SEEDLINGS AND INITIAL GROWTH OF JACARANDA MIMOSIFOLIA D. DON. (BIGNONIACEAE)

Revista Árvore ◽

10.1590/1806-90882018000400003 ◽

2018 ◽

Vol 42 (4) ◽

Author(s):

Jamille Rabêlo de Oliveira ◽

Clark Alberto Souza da Costa ◽

Antonio Marcos Esmeraldo Bezerra ◽

Haynna Fernandes Abud ◽

Eliseu Marlônio Pereira de Lucena

Keyword(s):

Quality Index ◽

Dry Weight ◽

Initial Growth ◽

Randomized Design ◽

Germination Process ◽

Length Width ◽

Number Of Leaves ◽

Collar Diameter ◽

Jacaranda Mimosifolia

ABSTRACT This research aimed to characterize the biometry of seeds, the morphology of seedlings and to evaluate the initial growth of blue jacaranda trees (Jacaranda mimosifolia D. Don.) in two environments with distinct luminosity. The biometry of 100 de-winged seeds was measured by their length, width and thickness. One hundred seeds were sown into 300 cm3 tubes and seedlings morphology was registered throughout the germination process using digital photographs disposed sequentially according to seedlings age. The initial growth analysis was performed in two environments, full sun and greenhouse, during nine periods, and arranged in a complete randomized design with subplots. The response variables were: number of leaves, plant height, collar diameter, root length, aerial and roots dry weight, and Dickson’s Quality Index (DQI). Biometry results showed seeds 7.09 - 9.26 mm long, 6.74 -9.39 mm wide, and 1.11 -1.89 mm thick. Germination is of hypogealphanerocotylar type, with first root arising six days after sown. Plants at full sun presented larger collar diameters, greater root dry weight and higher DQI as evaluation periods passed by. In conclusion, the biometry of seeds and the morphology of seedlings of blue jacaranda can be used to recognize this species in the field. Plants at full sun presented better development. Seedlings cultivated at full sun can be destined to recuperate forests and degraded areas just 36 weeks after sowed.

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Germination and Seed Vigour of Indigenous Bean (Phaseolus Vulgaris) Genotype in Nepal

SAARC Journal of Agriculture ◽

10.3329/sja.v18i2.51109 ◽

2021 ◽

Vol 18 (2) ◽

pp. 67-75

Author(s):

Anupam Uprety ◽

Bhishma Raj Dahal ◽

Bishal Shrestha

Keyword(s):

Phaseolus Vulgaris ◽

Seed Germination ◽

Germination Percentage ◽

Vegetable Crop ◽

Seed Vigour ◽

True Leaf ◽

Leaf Emergence ◽

Randomized Design ◽

Vigour Index ◽

Completely Randomized Design

Bean is an important vegetable crop of Nepal but farmers of Jumla district of Nepal are experiencing low productivity, probably due to poor germination. Keeping these points in view, a study was conducted to determine the germination and seed vigour of various indigenous beans genotype of Jumla district during February to March, 2018. The promising lines of Jumli beans namely KBL-3, KBL-2, PB0002, PB0001, KBL-1 and farm saved KBL-3 were used under study. The experiment was laid in completely randomized design (CRD) with four replications and six treatments namely T1 = KBL-3, T2 = KBL-2, T3 = PB0002, T4 = PB0001, T5 = KBL-1 and T6 = Farm-saved KBL-3. Significant differences were observed in germination percentage, speed of germination, seed vigourindex and true leaf emergence among different lines of Jumli beans .The highest germination percentage (96%), seed vigour index (1367) and speed of germination (1.763) was found in the variety of KBL-3 whereas the lowest germination percentage (69%), seed vigour index (947) and speed of germination (0.968) were found in the variety of KBL-1. Days to true leaf emergence was found shortest in the KBL-3 (23.50) and longest in the farm saved KBL-3 (30.25). KBL-3 pureline of Jumli bean was found to be highly responsive to seed germination and vigour characteristics. Thus, it would be better to suggest the indigenous bean growing farmers of Jumla district to cultivate KBL-3 for better germination and seed vigour. SAARC J. Agri., 18(2): 67-75 (2020)

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Effects of gamma rays on germination and growth in Jatropha curcas L.

Journal of Applied and Natural Science ◽

10.31018/jans.v7i2.715 ◽

2015 ◽

Vol 7 (2) ◽

pp. 964-969 ◽

Cited By ~ 1

Author(s):

Dileswar Nayak ◽

N. S. Patil ◽

L. K. Behera ◽

D. B. Jadeja

Keyword(s):

Leaf Area ◽

Gamma Rays ◽

Jatropha Curcas ◽

Seedling Survival ◽

Germination Percentage ◽

Shoot Length ◽

Growth And Yield ◽

Jatropha Curcas L ◽

Number Of Leaves ◽

Collar Diameter

The present investigation was carried out at the Forestry Research Farm, Navsari Agricultural University; Navsari to evaluate the gamma rays at 10 kR, 20 kR and 30 kR induced variability in Jatropha curcas L. on germination, growth and yield for seven Jatropha genotypes (Phule J-1, Urlikanchan, Hansraj, SKN Big, Chhatrapati, Hansot and MPJ-55). The significantly maximum germination percentage (66.96%), seedling survival (74.18%), seedling collar diameter (0.958cm), shoot length (49.442cm), number of leaves per seedling (7.757) and leaf area (37. 58)was observed in Chhatrapati genotype during nursery stage. While low rate of gamma rays treatment (10 kR) had stimulatory effect for germination percentage, seedling survival, seedling collar diameter, shoot length, number of leaves per seedling and leaf area. However, higher gamma rays doses (30 kR) drastically reduced all studied characters. The interaction effect of genotype and gamma rays were significant for number of leaves per seedling. Further, it was not significant in other traits like germination percentage, seedling survival, seedling collar diameter, shoot length and leaf area.

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Growth and Yield of Tomato (Lycopersicon esculentum Mill.) as Influenced by Poultry Manure and Biochar in Two (2) Soil Depths

Journal of Experimental Agriculture International ◽

10.9734/jeai/2020/v42i330484 ◽

2020 ◽

pp. 55-63

Author(s):

F. B. Musa ◽

F. O. Abiodun ◽

A. R. Falana ◽

B. H. Ugege ◽

R. V. Oyewumi ◽

...

Keyword(s):

Plant Height ◽

Poultry Manure ◽

Botanical Garden ◽

Growth And Yield ◽

Tomato Plants ◽

Randomized Design ◽

Significant Difference ◽

Number Of Leaves ◽

Collar Diameter ◽

And Control

Background and Objective: Unproductive soil has been a major problem to farmers, recent interest in the use of a stable organic manure (biochar) as soil amendment and poultry manure to increase soil fertility is also increasing. Hence, the experiment was conducted to assess the condition of poultry manure and biochar on the growth and yield of tomato. Materials and Methods: A greenhouse experiment was conducted at the Federal College of Forestry Ibadan, using polyethylene bag with 5 kg soil collected at two (2) soils depths, 0-15 cm and 15-30 cm depth at the botanical garden, Federal College of Forestry Ibadan. The experiment was arranged in a completely randomized design (CRD) with four replicates. The treatments used were poultry manure (5t/ha), biochar (10t/ha), mixture of poultry manure and biochar (1:1) and control (no amendment).The tomato seeds (UC82B) were sourced from NIHORT, Ibadan and raised in a germination basket. Data were collected weekly on plant height, collar diameter and number of leaves, 50% days to flowering and yield of tomato plants from two weeks after transplanting (WAT). Data collected were analyzed statistically using ANOVA while means were separated using LSD (p<0.05) where applicable. Results: In 0-15 cm soil, the growth variables (plant height, number of leaves and collar diameter) increased with various treatments used. The highest tomato height was observed with the application of biochar (10t/ha). All the treatments applied affected the number of tomato leaves at all weeks after transplanting (WAT) with poultry manure 5t/ha having the highest number of leaves at 7WAT, while collar diameter was significantly affected by various treatments from 2 to 5 WAT, with the mixture of P.M + BIO (1:1) recording the highest value and the control plot recorded the least values in all weeks. However, in 15-30 cm soil, there was significant difference in the number of leaves of tomato among all the amended soil from 5 to 7 WAT, the highest tomato leaves were obtained when the soil was amended with poultry manure (5t/ha) and can be compared with other treatments. Interaction of poultry manure and biochar(1:1) and poultry manure 5 t/ha resulted in highest height of tomato plant from 3-7 WAT as compared to the control that had the least value but were not significantly different from the sole application of biochar 10t/ha. Interaction of poultry manure + biochar (1:1) and poultry manure (5t/ha) gave the highest tomato flowering and fruits. Conclusion: Based on this result, biochar and poultry manure when added to soil, boosted the mineralization of the soil after amendment.

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PLANT GROWTH OF EGGPLANT (Solanum melongena L.) IN VITRO IN DROUGHT STRESS POLYETHYLENE GLYCOL (PEG)

BIOVALENTIA Biological Research Journal ◽

10.24233/biov.2.1.2016.29 ◽

2016 ◽

Vol 2 (1) ◽

Author(s):

Erna Siaga ◽

Awang Maharijaya ◽

Megayani Sri Rahayu

Keyword(s):

Polyethylene Glycol ◽

Drought Stress ◽

Plant Growth ◽

Solanum Melongena ◽

Dry Land ◽

Survival Percentage ◽

Drought Tolerant ◽

Randomized Design ◽

Number Of Leaves

Drought stress is one of the important issues related to the global warming that demand for the development of drought tolerant crops. Eggplant is one of the agricultural commodities which can be developed in dry land so plant growth of eggplant need to be learned. The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, and to find the drought tolerant eggplant accessions in dry land. The experiment was conducted at the Laboratory of Tissue Culture, Department of Agronomy and Horticulture, Bogor Agricultural University. The experiment was laid on a completely randomized design with one factor. The factor was eggplant accessions. The results showed that PEG in vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Callus in eggplant explants as a way of avoiding drought stress.

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