scholarly journals Comparative Physiology of Drought and Salinity Stress in Grass Pea (Lathyrus sativus L.) Seedlings

2021 ◽  
pp. 111-119
Author(s):  
P. Chettri ◽  
Kousik Atta ◽  
A. K. Pal
Keyword(s):  
Drought Stress ◽  
Salinity Stress ◽  
Osmotic Potential ◽  
Negative Impact ◽  
Laboratory Research ◽  
Sand Culture ◽  
Grass Pea ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Impact

Aims: The aim of this study was to investigate the effect of iso-osmotic potentials of drought and salinity on physiological parameters of grass pea seedlingsas well as to compare varietal responses. Study Design: Completely randomized design. Place and Duration of Study: In the years 2017-2018 and 2018-2019, laboratory research on grass pea varieties BK-14 and Pratik was conducted in the Department of Plant Physiology, Bidhan Chandra Krishi Viswavidyalaya, Nadia, West Bengal, India. Methodology: The effect of iso-osmotic potential of salinity and drought stress was studied using NaCl (50, 100 and 200 mM ) and PEG 6000 (10, 12 and 18%) solutions with -0.2, -0.4, and -0.8 MPa osmotic potential, and the experiment was carried out in sand culture using modified Hoagland solution under diffused light, at about 80±1% relative humidity (R.H.) and a temperature of 22±1oC. Data on different physiological and biochemical parameters were recorded after ten days of seedling growth in sand culture. Statistical analysis was performed on the mean data in all cases following completely randomized design (CRD) by application of INDOSTAT version 7.1 software. Results: The germination of grass pea seeds was more severely affected by drought stress than salinity. Both stresses had a negative impact on most of the parameters studied except for leaf proline and sugar The impact became  more pronounced as the severity of the stress increased. The highest intensity of drought stress was found to be more detrimental to leaf protein and relative water content in BK 14, while Pratik was more drastically affected by the highest level of salinity. Drought was found to have a significant negative impact on leaf starch in both the grass pea varieties. The highest concentration of PEG led to a remarkable increase in leaf proline. Conclusion: The mild to moderate levels (-0.2 and -0.4 MPa)  of stress did not produce much severe effects on the grass pea seedlings, but the highest intensity of stress with an osmotic potential of -0.8 MPa mostly produced drastic effects. There were varietal differences in response to two abiotic stresses. In general, drought stress was found to cause more negative effects on seedling than iso-osmotic potential of salinity stress.

scholarly journals Physiological and Biochemical Changes under Salinity and Drought Stress in Ricebean [Vigna umbellata (Thunb.) Ohwi and Ohashi] Seedlings

2020 ◽  
pp. 58-64
Author(s):  
Kousik Atta ◽  
P. Chettri ◽  
A. K. Pal
Keyword(s):  
Drought Stress ◽  
Salinity Stress ◽  
Seedling Growth ◽  
Osmotic Potential ◽  
Soluble Sugar ◽  
Sand Culture ◽  
Leaf Chlorophyll ◽  
Diffused Light ◽  
Osmotic Potentials ◽  
Physiological And Biochemical

Aims: To study the effect of iso-osmotic potentials of drought and salinity during seedling growth stage in ricebean. Study Design: Completely randomised design. Place and Duration of Study: The lab experiment was conducted during the year of 2017- 2018 and 2018-2019 in ricebean variety Bidhan 1 at Department of Plant Physiology, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia, West Bengal, India. Methodology: For studying the effect of iso-osmotic potential of salinity and drought stress, the solutions of NaCl and PEG 6000 with -0.2, -0.4 and -0.8 MPa osmotic potential were used and the experiment was conducted in sand culture using modified Hoagland solution [1] under laboratory condition of diffused light, at around 80±1% relative humidity (R.H.) and at a temperature of 28±1°C. Results: All the biochemical parameters under study, in general were adversely affected by the both stress with the effects being more drastic as the intensity of stress increased. The highest intensity of salinity stress was found to produce more adverse effects than drought in respect of RLWC, leaf chlorophyll as well as protein content in leaves of ricebean in the present experiment. While the content of soluble sugar, starch and phenol in the leaf were more drastically affected by drought stress. Conclusion: The drought stress was found to register more drastic effects on seedling growth as compared to iso-osmotic potential of salinity stress, especially, at the highest intensity of stress in ricebean cultivar Bidhan 1.

​Antioxidant Responses of Ricebean [Vigna umbellata (Thunb.) Ohwi and Ohashi] Seedling under Iso-osmotic Potential of Salinity and Drought Stress

2021 ◽  
Author(s):  
Kousik Atta ◽  
Jahnavi Sen ◽  
Pravachan Chettri ◽  
Anjan Kumar Pal
Keyword(s):  
Drought Stress ◽  
Salinity Stress ◽  
Osmotic Potential ◽  
West Bengal ◽  
Constitutive Expression ◽  
Growth And Yield ◽  
Antioxidant Responses ◽  
Nutrient Imbalance ◽  
Vigna Umbellata ◽  
Osmotic Potentials

Background: Salinity and drought are the major abiotic stresses and both can cause osmotic imbalances. Drought stress directly results in osmotic stress whereas salinity problem firstly disrupts the water balance and eventually induces ion toxicity which results in cyto-toxicity, metabolic impairment, nutrient imbalance and finally poor crop growth and yield. The co-ordinated up-regulation or constitutive expression of antioxidative system in plants is the main defense in plant against these stresses and thus the present experiment was undertaken to study the antioxidant responses under drought and salinity stress at seedling stage in ricebean (Bidhan 1). Methods: For studying the effect of iso-osmotic potential of salinity and drought stress solutions of NaCl and PEG 6000 with -0.2 MPa (50mM NaCl and 10% PEG), -0.4 MPa (100 mM NaCl and 12% PEG) and -0.8 MPa (200mM NaCl and 18% PEG) osmotic potential were used. The experiment was done in the laboratory of Department Plant Physiology, Bidhan Chandra Krishi Viswavidyalaya (BCKV), Mohanpur, Nadia and West Bengal in the year 2017-18 and 2018-19. Result: Under moderate to high intensity of osmotic stresses the leaf proline content decreased. The mild and medium stress treatments induced much higher activity of GPOX and APX in the leaf which then decreased somewhat as the intensity of stress increased. The experiment showed that drought stress was found to produce more drastic effects on seedling growth in ricebean as compared to the salinity stress at iso-osmotic potentials.

scholarly journals The impact of Caesalpinia sappan L. powder addition on the quality attributes of white and milk chocolates

2021 ◽  
Vol 332 ◽  
pp. 01002
Author(s):  
Elinda Okstaviyani ◽  
Kawiji ◽  
Raden Baskara Katri Anandhito ◽  
Asri Nursiwi ◽  
Dimas Rahadian Aji Muhamnmad
Keyword(s):  
Quality Attributes ◽  
Physical Analysis ◽  
Wood Powder ◽  
Polyphenol Content ◽  
Caesalpinia Sappan ◽  
Randomized Design ◽  
Powder Addition ◽  
Completely Randomized Design ◽  
The Impact

Sappan wood (Caesalpinia sappan L.) is a spice that has a high polyphenol content and has the potential to enrich the chocolate taste. This study evaluated the panelists’ acceptance and physical analysis (color and hardness) of white and milk compound chocolate with the addition of Sappan wood powder (0 %, 5%, 10%, 15%) by implementing a completely randomized design (CRD) experiment with one factor. The results showed that the addition of Sappan wood powder could reduce the panelists’ preference at the parameters of color, aroma, taste, texture and overalls. Hence, Panelists preferred white and milk compound chocolate without the addition of Sappan wood powder. Results of the physical analysis showed that the addition of Sappan wood powder decreased the chocolate brightness and increased the chocolate hardness.

scholarly journals Motilitas dan viabilitas spermatozoa semen sexing kambing peranakan etawa (pe) menggunakan metode sedimentasi putih telur dengan pengencer yang berbeda

2017 ◽  
Vol 5 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Yanpiterson Umbu Lele ◽  
Enike Dwi Kusumawati ◽  
Aju Tjatur Nugroho Krisnaningsih
Keyword(s):  
Significant Influence ◽  
Research Method ◽  
Egg Yolk ◽  
Egg White ◽  
Variance Analysis ◽  
Laboratory Research ◽  
Sedimentation Method ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRAK Tujuan penelitian ini adalah untuk mengetahui motilitas dan viabilitas spermatozoa semen hasil sexing kambing Peranakan Etawa (PE) dengan metode sedimentasi putih telur menggunakan pengencer yang berbeda. Materi yang digunakan dalam penelitian ini adalah semen segar kambing Peranakan Etawa (PE) berumur 2 tahun dan bobot badan 120 kg dari Balai Besar Inseminasi Buatan (BBIB)  Singosari Malang. Metode penelitian ini yang digunakan adalah penelitian laboratorium dengan menggunakan rancangan acak lengkap (RAL). Perlakuan terdiri dari semen sexing dengan menggunakan pengencer Andromed lapisan atas dan Andromed lapisan bawah dan Tris Aminomethan Kuning Telur lapisan atas dan Tris Aminomethan Kuning Telur lapisan bawah dengan masing-masing 10 ulangan. Variabel yang diamati adalah motilitas dan viabilitas spermatozoa semen sexing dengan pengencer yang berbeda, proses sexing menggunakan metode sedimentasi putih telur. Data yang diperoleh dianalisis menggunakan Analisis Varian  (ANOVA) apabila perlakuan memberikan perbedaan, maka dilanjutkan dengan Uji BNT. Hasil penelitian menunjukkan bahwa metode sedimentasi putih telur dengan pengencer yang berbeda pada kambing Peranakan Etawa (PE) memberikan pengaruh yang sangat nyata (P<0,01) terhadap motilitas dan viabilitas spermatozoa semen sexing. Motilitas dan viabilitas terbaik terdapat pada pengencer Tris aminomethan kuning telur lapisan atas sebesar 75,65%, 74,41% dan Andromed lapisan atas sebesar 65,4%, 60%. Berdasarkan hasil penelitian dapat disimpulkan bahwa kualitas spermatozoa semen sexing kambing PE dengan menggunakan pengencer Tris aminomethan memberikan hasil terbaik terhadap motilitas dan viabilitas. Berdasarkan hasil penelitian ini maka disarankan agar menggunakan pengencer Tris aminomethan kuning telur sebagai pengencer sexing spermatozoa.   ABSTRACT The purpose of this study was to determine the motility and viability of PE sperm sexing with egg white sedimentation method using different diluents. This research method used was laboratory research using a completely randomized design (CRD) treatment PE sexing sperm. The results showed that the quality of PE sperm goat with various diluents showed a significant influence (P<0,01). The variables observed were motility and viability of sperm. The data obtained were analyzed using variance analysis (ANOVA) with complete randomized design (CRD). Motility and viability at best against Tris aminomethane top layer of egg yolk 65,4% and the top layer of Andromed 75,65%, 74,41%. Based on the results it is suggested that using Tris aminomethane yolk as a diluents sexing sperm. It can be concluded that the quality of sexing sperm goat PE by using tris aminomethane diluent gives the best result on motility and viability.

scholarly journals Doses and forms of Azospirillum brasilense inoculation on maize crop

2018 ◽  
Vol 22 (6) ◽  
pp. 373-377 ◽  
Author(s):  
José M. K. Santini ◽  
Salatiér Buzetti ◽  
Marcelo C. M. Teixeira Filho ◽  
Fernando S. Galindo ◽  
Daniel N. Coaguila ◽  
...  
Keyword(s):  
Azospirillum Brasilense ◽  
Low Cost ◽  
Leaf Tissue ◽  
Physical Characteristics ◽  
Maize Crop ◽  
Nutrient Contents ◽  
Randomized Design ◽  
Zn Content ◽  
Completely Randomized Design ◽  
The Impact

ABSTRACT In search of a more sustainable agriculture, the use of beneficial microorganisms has been highlighted, because they are low-cost and can reduce the use of fertilizers and increase grain yield. The present study aimed to evaluate the efficiency of A. brasilense inoculation and the best form and dose of inoculation in maize, measuring the impact on some physical characteristics and on its nutrition. The experiment was conducted in a greenhouse, in Ilha Solteira, SP, Brazil, in a completely randomized design, with four replicates and eight treatments: 1) control; 2) Seed 1x; 3) Seed 2x; 4) Soil 1x; 5) Soil 2x; 6) Leaf 1x; 7) Leaf 2x; 8) Seed 1x + Leaf 1x, respectively representing in each treatment the site and dose of application (1x, dose recommended by the manufacturer; 2x, twice the dose recommended by the manufacturer). No differences were found in any physical characteristics evaluated between treatments; however, for nutrient contents in the leaf tissue, there was effect on Zn content. It was concluded that, regardless of the presence of A. brasilense inoculation, forms or dose (in hybrid DKB 350), in general, there were no improvements in the characteristics evaluated.

scholarly journals Economic analysis of the finishing of lambs under confinement conditions using licuri cake ( Syagrus coronata Mart. Becc.)

2019 ◽  
Vol 20 ◽  
Author(s):  
Jonival Barreto COSTA ◽  
Ronaldo Lopes OLIVEIRA ◽  
Thadeu Mariniello SILVA ◽  
Ossival Lolato RIBEIRO ◽  
Rebeca Dantas Xavier RIBEIRO ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Dry Matter ◽  
Randomized Design ◽  
Economic Parameters ◽  
Completely Randomized Design ◽  
The Impact ◽  
Operation Cost ◽  
Different Levels ◽  
Ground Corn ◽  
Corn Grain

SUMMARY The present study endeavors to determine the impact of the inclusion of licuri tart in the diet of finishing lambs, Santa Inês mestizos, by evaluating the economic parameters. For this purpose, a total of 44 lambs, including both uncastrated as well as vaccinated and wormed lambs, with an average age of six months and a mean body weight of 21.2 kg ± 2.7 kg, were employed. Initially, the lambs were fed on the diet which consisted of 40% Tifton-85 hay and 60% of a concentrated mixture, mainly composed of ground corn grain, soybean meal, mineral premix, being further followed by the inclusion of the licuri cake at four different levels (a) 0.0, (b) 8.0,(c) 16.0, and (d) 24% with respect to the dry matter. Principally, the soybean meal and milled corn were replaced by the licuri cake with these levels, thereby constituting four treatments and 11 replicates (11 lambs per treatment) in a completely randomized design. Further, for subjecting the lambs to the above mentioned four different levels of treatment, the lambs were confined for 70 days. The analysis of various economic parameters revealed that upon including the licuri cake up to the level of 24%, the operation cost decreases, which in turn results in higher profitability. However, its cost at the time of use will eventually determine its use for feeding lambs, because of the possible price variations of the ingredients used to formulate the diets along with the model of production of the rural property.

scholarly journals Nanoparticle of Silver Nitrate (Ag2NO3) and Carbamate (CO2NH3 derivatives) For Vector Control of Aedes aegypti

2019 ◽  
Vol 125 ◽  
pp. 16005
Author(s):  
Mursid Raharjo ◽  
Agus Subagyo ◽  
Sulistyani
Keyword(s):  
Aedes Aegypti ◽  
Vector Control ◽  
Toxicity Test ◽  
Experimental Approach ◽  
Infectious Agent ◽  
Laboratory Research ◽  
Nano Silver ◽  
Randomized Design ◽  
New Form ◽  
Completely Randomized Design

The climate change has an impact on various lives, including in the vector breeding. The Aedes aegypti resistance as an infectious agent of dengue fever is harmful for life, especially to non-target organisms and the environment. Nano-silver (Ag2NO3), is a new form of engineering in vector control. The aim of this research was the toxicity test of nano-silver (Ag2NO3) and carbamate (CO2NH3 derivatives) on Aedes aegypti larvae. This was a laboratory research (true experiment), used for 3-4 instar stage larvae toxicity test . The number of samples was calculated using an experimental approach, with a completely randomized design. As simply, can formulated as follows, (t-1) (r-1)> 15. The amount of this samples were 14. The toxicity test with carbamate compounds, at concentration 0.01 mg / l as many as 80% larvae were death, whereas on concentrations 0.5 mg / l as many as 92% of the test animals were death. Toxicity test with nano-silver on concentration 0.3 mg / l, all of the test animals (100%) experienced were death. The results of the observations also showed that the instar more active in the low cocentration of Ag2NO3 (0.01 mg/l - 0.1 mg/l). The conclusion are, nano-silver is more effective than carbamate compounds, and there is a potential for resistance.

scholarly journals Biochar as soil amendment: Impact on chemical properties and corn nutrient uptake in a Podzol

2016 ◽  
Vol 96 (4) ◽  
pp. 400-412 ◽  
Author(s):  
A.B. Syuhada ◽  
J. Shamshuddin ◽  
C.I. Fauziah ◽  
A.B. Rosenani ◽  
A. Arifin
Keyword(s):  
Nutrient Uptake ◽  
Chemical Properties ◽  
Total N ◽  
Fertilized Soil ◽  
Randomized Design ◽  
Exchangeable Al ◽  
Completely Randomized Design ◽  
Healthy Growth ◽  
The Impact ◽  
Biochar Amendment

A study was conducted to investigate the impact of biochar amendment on chemical properties and corn nutrient uptake in a sandy Podzol soil. Four rates of biochar (0, 5, 10, and 15 g kg−1) and two rates of inorganic fertilizer (0 and local recommendation rate for corn) were randomly applied to a completely randomized design with four replicates. Corn was grown for 45 d in a glasshouse using sandy Podzol. The increase in pH of the soil was concomitant with a decrease of exchangeable Al. The fertilized soil significantly increased total N, with a concomitant decrease in soil pH due to a N nitrification. Positive changes did occur in the soil due to biochar application, leading to a significant increase in dry matter yield and corn height. Corn N and K uptakes were significantly increased by the addition of biochar, but the same was not true for Ca and Mg. However, it was found that the concentrations of N, Ca, and Mg in the corn tissue were still lower than their critical level. Our results demonstrate that application of biochar alone is not able to supply enough nutrients for the healthy growth of corn.

scholarly journals Respon Anatomi dan Fisiologi Akar Kedelai terhadap Cekaman Kekeringan

2016 ◽  
Vol 43 (3) ◽  
pp. 186
Author(s):  
Pienyani Rosawanti ◽  
Munif Ghulamahdi ◽  
Nurul Khumaida
Keyword(s):  
Drought Stress ◽  
Lipid Peroxides ◽  
Root Damage ◽  
Demand And Supply ◽  
Anatomical Changes ◽  
Anatomy And Physiology ◽  
Randomized Design ◽  
Two Factors ◽  
Soybean Genotypes ◽  
Completely Randomized Design

<p>ABSTRACT</p><p>Drought stress in crop occurs due to imbalance between demand and supply of water. Crop responses to drought stress depend on their genotypes. Drought stress causes anatomical changes and root damage. The objective of this research was to study the effect of drought stress on anatomy and physiology of soybean root. The research was conducted at Laboratory of Microtechnique, Department of Agronomy and Horticulture, Bogor Agricultural University in November 2013. A Completely Randomized Design with two factors was used in the research i.e. soybean genotypes (Wilis, PG 57-1, SC 39-1) and PEG (0, 20%) with three replications. The result showed that genotype and PEG affected the size of the cortex, stele, and xylem. PG 57-1 genotype showed the decrease of cortical thickness, diameter of stele and the diameter of xylem, on the other hand, in SC 39-1, there was an increase. Drought stress (PEG 20%) could also cause damage to the root membrane as indicated by the occurrence of lipid peroxides. Wilis and PG 57-1 were tolerant while SC 39-1 was sensitive to drought stress.</p><p>Keywords:cortex, lipid peroxides, PEG, Schiff’s reagent, xylem</p>

scholarly journals Performance of perisel as shelter and periphyton substrate in the floating cage of vanname shrimp culture

2021 ◽  
Vol 20 (1) ◽  
pp. 65-71
Author(s):  
Rifka Liling Palinggi ◽  
Tatag Budiardi ◽  
Daniel Djokosetiyanto
Keyword(s):  
Negative Impact ◽  
Initial Density ◽  
Shrimp Farming ◽  
Production Performance ◽  
Feed Conversion ◽  
Shrimp Culture ◽  
Randomized Design ◽  
Significant Difference ◽  
Feed Source ◽  
Completely Randomized Design

Shrimp culture in the floating cage is expected to reduce the utilization of land and its possible negative impact to the environment. The advantages of shrimp farming in the sea include the high dissolved oxygen concentration and the better meat quality. This research aimed to enhance the production performance of shrimp through the utilization of periphyton as a natural feed for shrimp. A completely randomized design with 3 treatments (in triplicates) were applied in this experiment, i.e floating cage without perishel (control), floating cage with PE perishel and PA perishel. Shrimp with body weight of 2.5 ± 0.2 g were stocked at the initial density of 2,000 shrimp in each cage, and maintained for 90 days. No significant difference was observed in the survival amongst treatments. The lowest feed conversion ratio (1.74) and coefficient of variance (3.21) were showed in treatment PA. The highest attachment and abundances of periphyton were found in treatment PA. It was concluded that the addition of perishel inside the floating cage may contribute as natural feed source for the shrimp and thus increase the production performance of shrimp. Keywords: floating cage, Litopenaeus vannamei, periphyton, perisel, shelter ABSTRAK Budidaya udang di KJA diharapkan dapat menekan isu pemanfaatan daratan sebagai tambak yang berdampak pada permasalahan lingkungan. Keunggulan laut untuk budidaya udang, antara lain adalah kadar oksigen terlarut relatif tinggi sehingga tidak perlu kincir, dan mutu daging udang yang dihasilkan relatif lebih baik. Penelitian ini ditujukan untuk meningkatkan kinerja produksi dan memanfaatkan kesuburan perairan laut berupa perifiton sebagai pakan alami bagi udang. Penelitian ini terdiri atas tiga perlakuan, yakni: (A) kontrol (tanpa perishel), (B) jaring benang nilon (PE), dan (C) jaring benang serabut pendek (PA 6.6), masing-masing perlakuan terdiri dari tiga ulangan. Udang dengan bobot 2,5 ± 0,2 g ditebar sebanyak 2.000 ekor perwadah, dan dipelihara selama 90 hari. Hasil analisis kinerja produksi menunjukkan bahwa kelangsungan hidup tidak berbeda nyata antarperlakuan. Nilai konversi pakan terendah diperoleh pada perlakuan jaring benang PA dengan nilai 1,74. Nilai koefisien keragaman terendah diperoleh pada perlakuan jaring benang PA dengan nilai 3,21. Penempelan dan kepadatan perifiton yang cukup baik diperoleh pada jenis perishel jaring benang serabut pendek PA. Dengan demikian dapat disimpulkan bahwa dengan penambahan perishel sebagai shelter dan penumbuh perifiton diperoleh hasil produksi yang baik. Udang dapat memanfaatkan keberadaan perishel dalam wadah pemeliharaan sebagai tempat berlindung dan memperoleh makanan tambahan berupa pakan alami yang menempel pada perishel. Kata kunci: karamba jaring apung, perifiton, perisel, selter, udang vaname

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