FUNCTIONING OF NO-CYCLE IN THE SALIVA OF CHILDREN WITH TYPE 1 DIABETES MELLITUS

Introduction. The presence of type 1 diabetes mellitus in children is a major risk factor for periodontal disease. The aim of research work was to determine the activity of NO-synthase and arginase in saliva in children age with insulin-dependent diabetes mellitus. Materials and methods We examined 82 children including 56 children with type 1 diabetes mellitus and 26 children without somatic diseases. NO-synthase (NOS) activity was determined by the difference in nitrite concentration before and after incubation of tissue homogenate. Determining arginase activity was based on analysis the difference in the concentration of L-ornithine before and after incubation in phosphate buffer solution. Statistical processing was performed using Microsoft Office Excel. Research results and their discussion The violation of the indicators’ balance between groups 1 and 3 showed us a decrease in regenerative capacity in the mucous membrane in persons with type 1 diabetes mellitus. Increased ARG activity in group 4 children may lead to rivarly between NOS and ARG for L-arginine. Increased ARG activity in groups 2 and 4 compared with group 1 indicated an adaptive response aimed at repairing gum damage. Based on this, increased NO production from NOS is a consequence of insulin deficiency (systemic factor). Conclusions. The combination of systemic factor (type 1 diabetes mellitus) and local (chronic catarrhal gingivitis) leads to dysregulation of the NO-cycle and increasing of competition between NOS and ARG. ФУНКЦИОНИРОВАНИЕ NO-ЦИКЛА В СЛЮНЕ У ДЕТЕЙ С САХАРНЫМ ДИАБЕТОМ І ТИПА Кузь И.А., Акимов О.Е., Костенко В.А., Шешукова О.В., Максименко А.И., Писаренко Е.А. Полтавский государственный медицинский университет Вступление. Наличие у детей сахарного диабета І типа является основным фактором риска заболеваний пародонта. Целью исследования было определение активности NO-синтазы и аргиназы в слюне у детей с инсулинозависимым сахарным диабетом. Материалы и методы. Обследовано 82 ребенка, в том числе 56 детей с сахарным диабетом І типа и 26 детей без соматических заболеваний. Активность NO-синтазы (NOS) определяли по разнице в концентрации нитрита до и после инкубации гомогената ткани. Определение активности аргиназы основывалось на анализе разницы в концентрации L-орнитина до и после инкубации в фосфатном буферном растворе. Статистическая обработка проводилась с использованием Microsoft Office Excel. Результаты исследований и их обсуждение. Нарушение баланса показателей между 1 и 3 группами свидетельствовало о снижении регенерационной способности слизистой оболочки у лиц с сахарным диабетом І типа. Повышенная активность ARG у детей группы 4 может привести к неравенству между NOS и ARG для L-аргинина. Повышенная активность ARG в группах 2 и 4 по сравнению с группой 1 указала на адаптивный ответ, направленный на восстановление повреждений десен. Исходя из этого, повышенная продукция NO из NOS является следствием дефицита инсулина (системный фактор). Выводы. Сочетание системного фактора (сахарный диабет І типа) и местного (хронический катаральный гингивит) приводит к нарушению регуляции NO-цикла и усилению конкуренции между NOS и ARG.

Professor Alexander Mozzhukhin (by the 100th anniversary of his birth)

Alexander Sergeevich Mozzhukhin was born on August 27, 1921, in Krasnsodar in a family of an employee. After graduating with honors from high school, A.S. Mozzhukhin became a student at the Kuibyshev Military Medical Academy and completed his medical education with honors at the military faculty of the 2nd Moscow Medical Institute. In 1943, A.S. Mozzhukhin came to the Department of Normal Physiology of S.M. Kirov Military Medical Academy, with whom he connected more than 30 years of his life and in which he went from adjunct to head of the department, and scientific secretary of the academic council of the academy. The scientific activity of A.S. Mozzhukhin began in the life-giving atmosphere, which was formed at the department during the leadership of Academician L.A. Orbeli and his closest assistant A.V. Lebedinsky. All scientific activity of A.S. Mozzhukhin is an organic and natural combination of fundamental problems, physiology, psychophysiology, human biology, and practical medicine. The main scientific direction of the Department of Normal Physiology under the leadership of A.S. Mozzhukhin was on the study of the interaction of afferent systems under exposure to extreme stimuli as well as on the study of human functional reserves. In addition, a team examined the physiological cost of pedagogical activity depending on the age, seniority, psychophysical characteristics of teachers, and type of training sessions. A.S. Mozzhukhin investigated the biological effects of ionizing radiation. Together with chemist F.Yu. Rachinsky, he created a radio defense drug RS-1 and became a leading Soviet radiobiologist. A.S. Mozzhukhin created a unique scientific physiological school of the S.M. Kirov Military Medical Academy, which scientifically proved that the adaptation process was accompanied by the formation and improvement of a specific system of functional reserves for body adaptation, and the systemic factor was the result of the activity (adaptation). A.S. Mozzhukhin, while working at P.F. Lesgaft Institute of Physical Culture, proved that functional reserves have potentials in changing the functional activity of structural elements of the body and their interaction among themselves to achieve the target result, adapt to physical and psychoemotional loads, as well as the effect of various factors of the external environment on the body. The bright memory of Alexander Sergeyevich Mozzhukhin will forever remain in the hearts of his students and followers.

A Hierarchical Process Model Links Behavioral Aging and Lifespan in C. elegans

Aging involves a transition from youthful vigor to geriatric infirmity and death. To study the relationship between youthful vigor and lifespan, we developed a new version of “the lifespan machine” that can simultaneously measure age-associated changes in behavior and lifespan at high precision in large populations. Across diverse interventions, we find that behavioral aging and lifespan are not parsimoniously explained as manifestations of a single underlying aging process. Instead, the correlation between youthful vigor and lifespan is better explained as the result of two partially independent aging processes that progress during adulthood under the influence of a shared, systemic factor. Our model provides a framework for separating the direct, targeted effects of lifespan-altering interventions from their broad, systemic effects—supporting future efforts in the rational design of clinical interventions in aging.

Chiral Dualism as a Unifying Principle in Molecular Biophysics

The origin and potential role of chiral asymmetry remain one of the most exciting issues in biology. In this paper we review the chirality of biological macromolecules, starting at the level of single molecules and continuing to the level of supramolecular assemblies. We discuss the physical and chemical consequences of the presence of chirality and their role in the self-organization and formation of structural hierarchies in cells. Homochirality may serve as an essential factor that invokes mechanisms required to control the formation of discrete structural hierarchies in macromolecules and macromolecular assemblies. Symmetry is of fundamental importance not only for all molecular biology as a systemic factor of its organization but also for pharmacology, as well as a systemic factor of drug stereospecificity.

Development of the state policy of ukraine in the sphere of legal regulation of digitalization

It is revealed that today digitalization is considered as a systemic factor of socioeconomic development. It is determined that the reasons for this are the synergetic and paradigmatic development of digitalization itself. Ensuring its effectiveness requires effective legal regulation. In this context, the state of such regulation in Ukraine and the directions of its development within the relevant state policy are studied.

National systems of entrepreneurship: goals of sustainability

PurposeNational systems of entrepreneurship (NSE) broadly act as a means of allocating resources driven by the constant search for opportunities at the individual level through the launching of new businesses and firms with such activities, and their results are governed by the specific institutional characteristics of each country. In contrast to the institutional emphasis on innovation systems, in which such institutions establish and regulate actions, institutions are only able to regulate those who act with the results stemming from such individual actions, the core driver of national entrepreneurship systems.Design/methodology/approachGiven the challenges faced by companies and societies in general over mitigating climate change, support for sustainable entrepreneurship is fundamental. However, there has to be any study of the impact of national entrepreneurship systems on sustainability. This research therefore analyses the impact of national entrepreneurship systems on the sustainability of countries.FindingsThe authors conclude that those countries deploying higher level national entrepreneurship systems return better results in terms of their sustainability.Originality/valueThe authors, thus, seek to contribute towards the academic throughout deepening the knowledge prevailing on the relationship between entrepreneurship and sustainability. The authors also seek to enable managers, entrepreneurs and politicians to grasp how entrepreneurship is a systemic factor, and it is at this level that it may make its greatest contribution to bringing about sustainability.

TGFβ-like DAF-7 acts as a systemic signal for autophagy regulation in C. elegans

In response to stress conditions, autophagy activity in multicellular organisms is systemically modulated to ensure maintenance of cellular homeostasis at an organismal level. Very little is known about the intercellular signals that elicit the long-range organism-wide autophagy response. Here we showed that during Caenorhabditis elegans development, loss of cuticle annular furrow collagens elicits autophagy in the hypodermis, intestine, and muscle. The cilia of sensory neurons with cuticle-localized endings are essential for triggering this systemic response. The TGFβ-like molecule DAF-7, which is secreted in part from a specific pair of ciliated neurons, acts as a systemic factor that activates a canonical TGFβ signaling pathway in distant tissues to induce autophagy. We also showed that AAK-2/AMPK and the STAT-like protein STA-2 act differentially in different tissues for autophagy activation. Our study reveals a circuit that senses and transduces the signal from the damaged cuticle to activate systemic autophagy during animal development.

It’s not you, it’s the design - common problems with patient monitoring reported by anesthesiologists: a mixed qualitative and quantitative study.

BACKGROUND Patient monitoring is critical for perioperative patient safety as anesthesiologists routinely make crucial therapeutic decisions from the information displayed on patient monitors. Previous research has shown that today's patient monitoring has room for improvement in areas such as information overload and alarm fatigue. The rationale of this study was to learn more about the problems anesthesiologists face in patient monitoring and to derive improvement suggestions for next-generation patient monitors. METHODS We conducted a two-center qualitative/quantitative study. Initially, we interviewed 120 anesthesiologists (physicians and nurses) about the topic: common problems with patient monitoring in your daily work. Through deductive and inductive coding, we identified major topics and sub themes from the interviews. In a second step, a field survey, a separate group of 25 anesthesiologists rated their agree- or disagreement with central statements created for all identified major topics. RESULTS We identified the following six main topics: 1. "Alarms," 2. "Artifacts," 3. "Software," 4. "Hardware," 5. "Human Factors," 6. "System Factors," and 17 sub themes. The central statements rated for the major topics were: 1. “problems with alarm settings complicate patient monitoring.” (96% agreed) 2. “artifacts complicate the assessment of the situation.” (56% agreed) 3. “information overload makes it difficult to get an overview quickly.” (56% agreed) 4. “problems with cables complicate working with patient monitors.” (88% agreed) 5. “factors related to human performance lead to critical information not being perceived.” (64% agreed) 6. “Switching between monitors from different manufacturers is difficult.” (88% agreed). The ratings of all statements differed significantly from neutral opinion (all p<0.03). CONCLUSION This study provides an overview of the problems anesthesiologists face in patient monitoring. Some of the issues, to our knowledge, were not previously identified as common problems in patient monitoring, e.g., hardware problems (e.g., cable entanglement and worn connectors), human factor aspects (e.g., fatigue and distractions), and systemic factor aspects (e.g., insufficient standardization between manufacturers). An ideal monitor should transfer the relevant patient monitoring information as efficiently as possible, prevent false positive alarms, and use technologies designed to improve the problems in patient monitoring.