scholarly journals Pharmacognostical, Phytochemical and Gas Chromatography Mass Spectroscopy Profiling of Stenosiphonium russellianum Nees

2021 ◽  
pp. 95-104
Author(s):  
G. Mahalakshmi ◽  
B. Kavitha ◽  
N. Balachandran ◽  
S. Kavimani
Keyword(s):  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Diethyl Ether ◽  
Herbal Remedy ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
The Family ◽  
Gas Chromatography Mass Spectroscopy ◽  
Activity Estimation ◽  
Macroscopic Analysis

Stenosiphonium russellianum Nees. is rarely known species belongs to the family Acanthaceae and it is a shrub, found above 500m on slopes of mountain. It was traditionally used for wound healing in and as blood purifier. The current study designed to provide the requisite pharmacognostical and phytochemical properties of Stenosiphonium russellianum. Pharmacognostical studies like microscopic and macroscopic analysis of the leaves were carried out. Physiochemical parameter and preliminary phytochemical screening for secondary metabolite were also performed. Extracts were taken from nonpolar to polar solvants like hexane, diethyl ether, ethyl acetate, alcohol and water. Their extractive values are calculated. GCMS analysis of hexane, diethyl ether, ethyl acetate and ethanol extract of the leaves of Stenosiphonium russellianum were studied. Preliminary phytochemical evaluation showed the presence of alkaloids, phytosterols and glycosides. GCMS analysis revealed the presence compounds like lupeol, gamma sitosterol and stigmasterols. In conclusion, the information obtained from these studies can be used as markers in the identification and standardization of this plant as an herbal remedy and also towards further pharmacological activity estimation.

scholarly journals Phytochemical Screening and GC-MS Chemical Profiling of Ethyl Acetate Extract of Seed and Stem of Anethum sowa Linn.

2018 ◽  
Vol 16 (2) ◽  
pp. 187-194
Author(s):  
Muhammad Abdullah Al Mansur ◽  
M Mahboob Ali Siddiqi ◽  
Md Ahedul Akbor ◽  
Koushik Saha
Keyword(s):  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Phytochemical Screening ◽  
Chemical Profiling ◽  
Phytochemical Constituents ◽  
Gas Chromatography Mass Spectroscopy ◽  
Anethum Sowa ◽  
Qualitative Analyses ◽  
Ethyl Acetate Extracts

Tahe phytochemical constituents from the ethyl acetate extracts of seed and stem of Anethum sowa were identified by qualitative and gas chromatography-mass spectroscopy (GC-MS). Qualitative analyses exhibited the presence of alkaloids, flavonoids, tannins, carbohydrate, steroids and terpenoids in both extracts. In GC-MS analysis of A. sowa 6 notable peaks (3,4,4a,5,6,7,8,9-Octahydro-2H-benzocyclohepten-2-one, 2,2,4,6,7-Pentamethyl- 1,2,3,4-tetrahydro quinoline, 5-Ethyl-2-methyl-pyridin-4-amine, 2-(2-Furyl) pyridine, 9-Ethyl 9-borabicyclo-[3.3.1]- nonane and 7-Methylenebicyclo-[4.2.0]-octane) and 5 significant peaks (3-Cyclopentyl-1-propyne, 2,2,4,6,7- Pentamethyl-1,2,3,4-tetrahydroquinoline,3,4,4a,5,6,7,8,9-Octahydro-2H-benzocyclohepten-2-one, 1,5-Naphthy-ridin- 2-amine and Octahydro-4,7-methano-5H-inden-5-one) with comparatively higher peak area (%) among 26 and 23 compounds were detected from the ethyl acetate extract of stem and seed respectively. The study encapsulates the information regarding the phytochemical constituents present in the extracts which may have pharmacological importance.Dhaka Univ. J. Pharm. Sci. 16(2): 187-194, 2017 (December)

scholarly journals Preliminary Phytochemical Screening of wild edible fruits from Boda and Kolli hills

2017 ◽  
Vol 3 ◽  
Author(s):  
S.P. Anand ◽  
S. Deborah
Keyword(s):  
Ethyl Acetate ◽  
Diethyl Ether ◽  
Ethanol Extract ◽  
Vital Role ◽  
Kolli Hills ◽  
Diethyl Ether Extract ◽  
Therapeutic Value ◽  
Phytochemical Compounds ◽  
Wild Edible Fruits ◽  
Phytochemical Constituent

<span>Medicinal plant plays a vital role for ailment of various chronic diseases due to the presence of phytochemical constituent. The preliminary phytochemical compounds was studied by using the ethanol, ethyl acetate, diethyl ether, chloroform and aqueous extracts of 15 wild edible fruits collected from <em>Boda </em>and <em>Kolli</em> Hills. The phytochemical compounds such as Phenol, tannins, saponin, alkaloid, flavonoids, steroids, </span><span lang="EN-IN">phlobotannins</span><span>, terpenoids, anthroquinones and cardiac glycosides were screened in 15 edible fruits by using standard methods. The diethyl ether extract, ethyl acetate and ethanol extract of the selected fruits<strong> </strong>shows the presence of phytochemicals compound. The highest occurrence shows the high therapeutic value possessing majority of phytochemical constituent of active compounds. In Further studies, the compound from these fruits were isolated and used for medicinal purposes</span>

scholarly journals IDENTIFICATION OF BIOACTIVE COMPONENTS IN ENHALUS ACOROIDES SEAGRASS EXTRACT BY GAS CHROMATOGRAPHY–MASS SPECTROMETRY

2018 ◽  
Vol 11 (10) ◽  
pp. 313 ◽  
Author(s):  
Amudha P ◽  
Jayalakshmi M ◽  
Pushpabharathi N ◽  
Vanitha V
Keyword(s):  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Property ◽  
Butylated Hydroxytoluene ◽  
Gas Chromatography Mass Spectrometry ◽  
Antitumor Activities ◽  
Enhalus Acoroides ◽  
Gas Chromatography Mass Spectroscopy

Objective: This study deals with the determination of possible phytocompounds present in the ethyl acetate extract of Enhalus acoroides using gas chromatography-mass spectroscopy (GC-MS) technique. Methods: Using GC-MS technique the phytocompounds present in the ethyl acetate extract of E. acoroides whole seagrass was investigated, and the mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology library.Results: GC-MS analysis of E. acoroides extract revealed the existence of several phytocompounds which includes 1-nonadecene (17.15%), n-tetracosanol-1 (11.48%), 1-octadecene (10.06%), 2-pentadecanone (7.87%), behenyl alcohol (7.33%), 17-pentatriacontene (4.84%), triacontane (4.25%), tetratetracontane (4.17%), and butylated hydroxytoluene (2.09%).Conclusion: E. acoroides possess distinct phytocompounds such as 1-nonadecene and n-tetracosanol-1 which possess antioxidant property, triacontane which has antibacterial, antidiabetic and antitumor activities. Further studies need to elute novel bioactive compounds and toxicity profile through animal models. 

scholarly journals THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT, FRACTIONS OF WATER, ETHYL ACETATE AND n-HEXANE FROM MISTLETOE TEA (SCURRULA ATROPURPUREABL. DANS)

2017 ◽  
Vol 10 (2) ◽  
pp. 343 ◽  
Author(s):  
Resmi Mustarichie
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Ic50 Values

Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted based on the method of Farnsworth.Results: The IC50 values of ethanol extract, water fraction, fraction of ethyl acetate, and n-hexane fraction were 21.92 ppm, 89.57 ppm, 14.08 ppm, and 162.09 ppm, respectively, whereas for ascorbic acid was 4.41 ppm. The ethanol extract and ethyl acetate fraction contained compounds were the same group, polyphenolic, tannins, flavonoids, monoterpenoid, steroids, triterpenoids, and quinones. Fraction of water contained compounds such as polyphenolic group, flavonoids, monoterpenoids, sesquiterpenoids, steroids, and triterpenoids. n-hexane fraction compounds contained steroids and triterpenoids.Conclusion: The ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction showed antioxidant activities. The ethanol extract, fractions of water, and ethyl acetate fraction contained flavonoids and polyphenolic potential as antioxidants.Keywords: Mistletoe tea, Scurrula atropurpurea, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ascorbic acid.

scholarly journals Phytochemical Screening and Toxicity Test from Extracts of Mahang (Macaranga bancana) Leaves

2019 ◽  
Vol 9 (2) ◽  
pp. 19-23
Author(s):  
Rianti Putri ◽  
Rudi Hendra Sy ◽  
Hilwan Yuda Teruna
Keyword(s):  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Phytochemical Screening ◽  
Wide Spread ◽  
Lc50 Value ◽  
Ethanol Toxicity ◽  
Hexane Extracts ◽  
Toxicity Analysis ◽  
Medicinal Properties

Macaranga bancana (Euphorbiaceae) known as “mahang” which is wide spread in Indragiri Hulu, Riau Province and also believed to has medicinal properties. This study to evaluate the secondary metabolites contents and toxicity activity from various extracts of M. bancana leaves. Extraction process were done by using maceration method with various solvents, such as n-hexane, dichloromethane, ethyl acetate, methanol, and ethanol. Toxicity analysis was done by Brine Shrimp Lethality Test (BSLT). The results of phytochemical screening showed that M. bancana leaves contain terpenoid, steroid, flavonoid and phenolic. Toxicity analysis showed that n-hexane extracts prossessed the highest level of toxicity followed by dichloromethane, ethyl acetate and methanol extracts with LC50 value of 65; 87; 227; 605 μg/mL, respectively while ethanol extract has not toxic. Therefore, it could be concluded that M. bancana has good toxicity level and could be used as screening for anticancer.

scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROSCOPY ANALYSIS OF ROOT OF AN ECONOMICALLY IMPORTANT PLANT, CENCHRUS CILIARIS L. FROM THAR DESERT, RAJASTHAN (INDIA)

2017 ◽  
Vol 10 (9) ◽  
pp. 64 ◽  
Author(s):  
Sunita Arora ◽  
Ganesh Kumar ◽  
Sonam Meena
Keyword(s):  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Mass Spectroscopy ◽  
Plant Root ◽  
Plant Secondary Metabolites ◽  
Maximum Amount ◽  
Cenchrus Ciliaris ◽  
Phytochemical Compounds ◽  
Traditional Practitioners ◽  
Gas Chromatography Mass Spectroscopy

Objective: The study was carried out with an objective to characterize the possible bioactive phytochemical constituents from the root of Cenchrus ciliaris L. using various solvents of different polarities, i.e., methanol, ethyl acetate, and hexane by gas chromatography-mass spectroscopy (GC-MS) analysis.Methods: Plant material was collected from harsh, xeric conditions of “Thar” during August to October. The shade-dried plant root powder was extracted with solvents using Soxhlet extractor. The phytochemical compounds were investigated using PerkinElmer GC-MS, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology and Willey 8 library.Results: Maximum % area is found for stigmasta-5,22-dien-3-ol and present in maximum amount (12.68%) with reaction time (RT)=36.461 minutes in the methanolic extract. 1,2,3-propanetriol, 1-acetate is present in maximum amount (13.15%) with RT=6.582 minutes in the ethyl acetate extract. Tetracontane is present in maximum amount (16.70%) with RT=18.744 minutes in the hexane extract of root of C. ciliaris L.Conclusions: Green plants synthesize and preserve a variety of biochemical compounds. Plant secondary metabolites are commercially important and are used by pharmaceutical industry as well as the traditional practitioners. The GC-MS study helps to predict the formula and structure of phytoconstituents that can be used for drug design, and further investigation may lead to the development of various drug formulations. 

BRINE SHRIMPS LETHALITY TEST OF ETHANOL EXTRACT AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHYL ACETATE FRACTION OF BLIGHIA SAPIDA

2020 ◽  
pp. 208-212
Author(s):  
ADEKOLA MB ◽  
AREOLA JO ◽  
ORIYOMI OV ◽  
APATA JT ◽  
APALOWO OE ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Blighia Sapida

Objective: The objective of the study was to evaluate brine shrimp lethality of Blighia sapida stem-bark extract and its fractions and identify the bioactive constituents in the ethyl acetate fraction (EAF) using gas chromatography-mass spectrometry (GC-MS) technique. Methods: The ethanol extract (EE) and its fractions were subjected to lethality assay, and GC-MS analysis of EAF was carried out. Results: The lethality test showed a concentration-dependent mortality rate in the brine shrimp nauplii for the EE and its fractions. GC-MS analysis of EAF of the extract revealed the existence of 13 peaks of the GC-MS chromatogram with only one prominent compound, n-hexadecanoic acid (peak area of 10.13%). Conclusion: The result revealed the presence of 13 bioactive components in the EAF of the extract, the majority of which have been reported for different biological activities, hence, justifies the use of the plant in the treatment and management of different diseases ethnomedicinally.

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