scholarly journals Phytochemical Screening and GC-MS Chemical Profiling of Ethyl Acetate Extract of Seed and Stem of Anethum sowa Linn.

2018 ◽  
Vol 16 (2) ◽  
pp. 187-194
Author(s):  
Muhammad Abdullah Al Mansur ◽  
M Mahboob Ali Siddiqi ◽  
Md Ahedul Akbor ◽  
Koushik Saha
Keyword(s):  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Phytochemical Screening ◽  
Chemical Profiling ◽  
Phytochemical Constituents ◽  
Gas Chromatography Mass Spectroscopy ◽  
Anethum Sowa ◽  
Qualitative Analyses ◽  
Ethyl Acetate Extracts

Tahe phytochemical constituents from the ethyl acetate extracts of seed and stem of Anethum sowa were identified by qualitative and gas chromatography-mass spectroscopy (GC-MS). Qualitative analyses exhibited the presence of alkaloids, flavonoids, tannins, carbohydrate, steroids and terpenoids in both extracts. In GC-MS analysis of A. sowa 6 notable peaks (3,4,4a,5,6,7,8,9-Octahydro-2H-benzocyclohepten-2-one, 2,2,4,6,7-Pentamethyl- 1,2,3,4-tetrahydro quinoline, 5-Ethyl-2-methyl-pyridin-4-amine, 2-(2-Furyl) pyridine, 9-Ethyl 9-borabicyclo-[3.3.1]- nonane and 7-Methylenebicyclo-[4.2.0]-octane) and 5 significant peaks (3-Cyclopentyl-1-propyne, 2,2,4,6,7- Pentamethyl-1,2,3,4-tetrahydroquinoline,3,4,4a,5,6,7,8,9-Octahydro-2H-benzocyclohepten-2-one, 1,5-Naphthy-ridin- 2-amine and Octahydro-4,7-methano-5H-inden-5-one) with comparatively higher peak area (%) among 26 and 23 compounds were detected from the ethyl acetate extract of stem and seed respectively. The study encapsulates the information regarding the phytochemical constituents present in the extracts which may have pharmacological importance.Dhaka Univ. J. Pharm. Sci. 16(2): 187-194, 2017 (December)

scholarly journals IDENTIFICATION OF BIOACTIVE COMPONENTS IN ENHALUS ACOROIDES SEAGRASS EXTRACT BY GAS CHROMATOGRAPHY–MASS SPECTROMETRY

2018 ◽  
Vol 11 (10) ◽  
pp. 313 ◽  
Author(s):  
Amudha P ◽  
Jayalakshmi M ◽  
Pushpabharathi N ◽  
Vanitha V
Keyword(s):  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Property ◽  
Butylated Hydroxytoluene ◽  
Gas Chromatography Mass Spectrometry ◽  
Antitumor Activities ◽  
Enhalus Acoroides ◽  
Gas Chromatography Mass Spectroscopy

Objective: This study deals with the determination of possible phytocompounds present in the ethyl acetate extract of Enhalus acoroides using gas chromatography-mass spectroscopy (GC-MS) technique. Methods: Using GC-MS technique the phytocompounds present in the ethyl acetate extract of E. acoroides whole seagrass was investigated, and the mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology library.Results: GC-MS analysis of E. acoroides extract revealed the existence of several phytocompounds which includes 1-nonadecene (17.15%), n-tetracosanol-1 (11.48%), 1-octadecene (10.06%), 2-pentadecanone (7.87%), behenyl alcohol (7.33%), 17-pentatriacontene (4.84%), triacontane (4.25%), tetratetracontane (4.17%), and butylated hydroxytoluene (2.09%).Conclusion: E. acoroides possess distinct phytocompounds such as 1-nonadecene and n-tetracosanol-1 which possess antioxidant property, triacontane which has antibacterial, antidiabetic and antitumor activities. Further studies need to elute novel bioactive compounds and toxicity profile through animal models. 

scholarly journals Pharmacognostical, Phytochemical and Gas Chromatography Mass Spectroscopy Profiling of Stenosiphonium russellianum Nees

2021 ◽  
pp. 95-104
Author(s):  
G. Mahalakshmi ◽  
B. Kavitha ◽  
N. Balachandran ◽  
S. Kavimani
Keyword(s):  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Diethyl Ether ◽  
Herbal Remedy ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
The Family ◽  
Gas Chromatography Mass Spectroscopy ◽  
Activity Estimation ◽  
Macroscopic Analysis

Stenosiphonium russellianum Nees. is rarely known species belongs to the family Acanthaceae and it is a shrub, found above 500m on slopes of mountain. It was traditionally used for wound healing in and as blood purifier. The current study designed to provide the requisite pharmacognostical and phytochemical properties of Stenosiphonium russellianum. Pharmacognostical studies like microscopic and macroscopic analysis of the leaves were carried out. Physiochemical parameter and preliminary phytochemical screening for secondary metabolite were also performed. Extracts were taken from nonpolar to polar solvants like hexane, diethyl ether, ethyl acetate, alcohol and water. Their extractive values are calculated. GCMS analysis of hexane, diethyl ether, ethyl acetate and ethanol extract of the leaves of Stenosiphonium russellianum were studied. Preliminary phytochemical evaluation showed the presence of alkaloids, phytosterols and glycosides. GCMS analysis revealed the presence compounds like lupeol, gamma sitosterol and stigmasterols. In conclusion, the information obtained from these studies can be used as markers in the identification and standardization of this plant as an herbal remedy and also towards further pharmacological activity estimation.

scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROSCOPY STUDIES ON CESTRUM NOCTURNUM MACERATED METHANOLIC EXTRACT

2017 ◽  
Vol 10 (3) ◽  
pp. 258263 ◽  
Author(s):  
Pratip Kashinath Chaskar ◽  
Shivali Hargovind Tank ◽  
Gaurav Mahesh Doshi
Keyword(s):  
Gas Chromatography ◽  
Mass Spectroscopy ◽  
Methanolic Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Screening ◽  
Hexadecanoic Acid ◽  
Extraction Procedures ◽  
Phytochemical Constituents ◽  
Gas Chromatography Mass Spectroscopy ◽  
Spectroscopy Studies

ABSTRACTObjective: Phytochemical screening has revealed the presence of alkaloids, glycosides, carbohydrates, steroids, flavonoids, saponins, and tannins frommethanolic extract of Cestrum nocturnum. In this research, we have identified and confirmed the structures of the constituents by gas chromatography–mass spectrometry (GC-MS) from the methanolic extract of C. nocturnum leaves.Methods: Shade-dried leaves were powdered and extracted with methanol by cold extraction procedures and the crude extract obtained was usedfor GC-MS.Results: There were total five components prominently eluted from C. nocturnum extract, viz., ethyl citrate, phytol, 4-isobutylmorpholine,n-hexadecanoic acid, and α-amyrin.Conclusion: GC-MS studies helped to recognize the phytochemical constituents based on their retention time and verification of MS libraries. Infuture, C. nocturnum leaves may be subjected to different extraction procedures and number of elucidating phytoconstituents can be studied for theirethnopharmacological relevance citing the literature.Keywords: Cestrum nocturnum, Gas chromatography-mass spectroscopy, α-Amyrin, n-Hexadecanoic acid, Ethyl citrate, Phytol, 4-Isobutylmorpholine.

scholarly journals PHYTOCHEMICAL AND ANTIBACTERIAL ACTIVITY OF HEXANE AND ETHYL ACETATE EXTRACT OF KNOXIA SUMATRENSIS (RETZ.) DC.

2021 ◽  
pp. 35-39
Author(s):  
LOGANATHAN S ◽  
SELVAM K
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Standard Procedure ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Disk Diffusion Method ◽  
Near Future ◽  
Ethyl Acetate Extracts

Objective: The study was carried out the phytochemical and antibacterial activity of Knoxia sumatrensis (Retz.) Dc. using hexane and ethyl acetate extracts. Methods: The phytochemical screening was extracted from hexane and ethyl acetate solvent and its screening was analyzed by standard procedure. GC-MS technique was analyzed in ethyl acetate extract to identify the components present in the extract. The hexane and ethyl acetate extract of the plant was tested for antibacterial activity against human pathogenic bacteria using disk diffusion method. Results: The phytochemical screening was revealed the presence of phenols, saponins, flavonoids, cardiac glycosides, steroids, and tannins. The GC-MS results showed that the presence of seven bioactive compounds in ethyl acetate extract. The major compounds were identified such as N-Hexadecanoic Acid (9.336), 2-Piperidinone, N-[4-Bromo-N-Butyl] - (37. 883). The ethyl acetate extract showed good antibacterial activity. The maximum zone of inhibition was noticed in S. aureus (8.25 mm) using ethyl acetate extracts at 50 μg. Conclusion: Thus, this study the information regarding the phytochemical constituents present in the both extract. Hence, it can be used for further therapeutic applications in the near future study.

scholarly journals Thrombolytic, Membrane Stabilizing and Hypoglycemic Activities of Anethum sowa Linn.

2018 ◽  
Vol 17 (2) ◽  
pp. 237-241
Author(s):  
Muhammad Abdullah Al Mansur ◽  
M Mahboob Ali Siddiqi ◽  
Koushik Saha
Keyword(s):  
Blood Glucose ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Hypotonic Solution ◽  
Thrombolytic Activity ◽  
Glucose Lowering ◽  
Glucose Lowering Effect ◽  
Anethum Sowa ◽  
Ethyl Acetate Extracts

The hexane (HE), dichloromethane (DCME), ethyl acetate (EAE) and methanol (ME) extracts of seed and stem of Anethum sowa were subjected to screenings for thrombolytic, membrane stabilizing and hypoglycemic activities. Ethyl acetate extract of stem showed highest thrombolytic activity. In addition, the dichloromethane, ethyl acetate and methanol extract of seed revealed higher percentage (%) of inhibition in hypotonic solution induced hemolysis. In hypoglycemic activity, the dichloromethane and ethyl acetate extracts of stem and seed displayed significant blood glucose lowering effect. Dhaka Univ. J. Pharm. Sci. 17(2): 237-241, 2018 (December)

Phytochemical screening for identification of bioactive compounds of Lantana camara Linn. (Verbenaceae) responsible for larvicidal action against Aedes triseriatus Say, 1823 (Diptera : Culicidae)

ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 117-126
Author(s):  
Rhitayu Chakraborti ◽  
Probir Kumar Bandyopadhyay
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Active Ingredient ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Lantana Camara ◽  
Mosquito Larvae ◽  
Aedes Triseriatus ◽  
Phytochemical Screening ◽  
Lc50 Value

Study to assess the larvicidal property of Lantana camara leaves against Aedes triseriatus larvae found that the ethyl acetate extract had profound larvicidal action with the crude extract having a LC50 value of 409.831ppm. GC-MS analysis of the ethyl acetate extract confirmed the presence of twenty-one compounds out of which beta-caryophyllene covered the highest percentage of the chromatogram area. Further tests with beta-caryophyllene against the mosquito larvae proved it to be the active ingredient of L. Camara with a LC50 value of 104.243ppm.

α-glucosidase Inhibitory Activity of Extracts and Compounds from the Leaves of Ruellia tuberosa L.

2021 ◽  
Vol 11 ◽  
Author(s):  
Le Nguyen Tuong Vi ◽  
Nguyen Ngoc Tuan ◽  
Quach Tong Hung ◽  
Pham Thi Nhat Trinh ◽  
Tong Thanh Danh ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Spectroscopic Method ◽  
Positive Control ◽  
Strong Activity ◽  
Butanol Extract ◽  
Glucosidase Inhibitor ◽  
Inhibitory Activities ◽  
Bio Assay ◽  
Ethyl Acetate Extracts

Background: In recent years, the study of the structure and biological activity of medicinal plants has a particularly important to search for diabetes medicine. Ruellia tuberosa is used to treat various diseases such as diabetes by inhibiting the activity of α-glucosidase. Objective: In this study, experiment was designed to isolated isolate and identified identify α-glucosidase inhibitory extracts and compounds from Ruellia tuberosa L. through bio-assay guided isolation. Method: Dry powder of Ruellia tuberosa L. was extracted with 70% ethanol, then liquid-liquid partition with n-hexane, ethyl acetate and butanol, respectively. The extracts were evaluated with α-glucosidase inhibition. The potential extracts were isolated and identified compounds. The effects of these compounds on the α-glucosidase inhibitory were evaluated. Results: The a-glucosidase inhibitory activities showed that the n-hexane, ethyl acetate and the butanol extract had the α-glucosidase inhibition with an IC50 of 46.2 0.9, 6.6 2.9 and 8.9  μg/mL, respectively. From the n-hexane and ethyl acetate extracts, the structures of four compounds were elucidated by NMR spectroscopic method, including lupeol (1), syringaresinol (2), apigenin (3), verbascoside (4). The a-glucosidase inhibitory activities showed that all isolated compounds were more active than the positive control - acarbose with an IC50 of 37.5  0.4; 19.5  0.2; 20.1  0.3; 69.3  0.2 µg/mL, respectively. Conclusion: The ethyl acetate extract showed strong activity about 19 times more than positive control - acarbose. The compound syringaresinol (2) was the most powerful α-glucosidase inhibitor. Therefore, Ruellia tuberosa L. contains many compounds that can inhibit α-glucosidase activity.

Vasodilatory effects and underlying mechanisms of the ethyl acetate extracts from Gastrodia elata

2017 ◽  
Vol 95 (5) ◽  
pp. 564-571 ◽  
Author(s):  
Rong Dai ◽  
Ting Wang ◽  
Xiaoqin Si ◽  
Yuanyuan Jia ◽  
Lili Wang ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Vascular Tone ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Rat Aorta ◽  
Gastrodia Elata ◽  
Vasodilatory Effect ◽  
Intracellular Ca ◽  
Underlying Mechanisms ◽  
Ethyl Acetate Extracts

The objective of this study was to assess the ethyl acetate extracts of Gastrodia elata Blume (GEB) on vascular tone and the mechanisms involved. GEB was extracted with 95% EtOH followed by a further extraction with ethyl acetate. The effects of GEB and its ingredients on the isometric tensions of the aortic rings from rats were measured. The ethyl acetate extract of GEB induced a vasodilatory effect on rat aorta, which was partially dependent on endothelium. Four chemical compounds isolated from GEB were identified as 3,4-dihydroxybenzaldehyde (DB), 4-hydroxybenzaldehyde (HB), 4-methoxybenzyl alcohol (MA), and 4,4′-dihydroxydiphenyl methane (DM), respectively. All of these compounds induced vasodilatations, which were dependent on the endothelium to different degrees. After pretreatment with Nω-nitro-l-arginine methyl ester, indomethacin, or methylene blue, the vasodilatations induced by DB, HB, and MA were significantly decreased. In addition, the contractions of the rat aortic rings due to Ca2+ influx and intracellular Ca2+ release were also inhibited by DM. Furthermore, the administration of DB significantly enhanced the productions of nitric oxide (NO) and the activities of the endothelial NO synthase in aorta and in endothelial cells. Thus, GEB may play an important role in the amelioration of hypertension by modulating vascular tones.

scholarly journals The Dose Effect of Mangrove Leaf Extract (Rhizophora apiculata) on Anticancer Activity in HeLa Cells

2021 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Dwi Mahfud Maulana
Keyword(s):  
Hela Cell ◽  
Cell Viability ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Rhizophora Apiculata ◽  
Ic50 Value ◽  
Ethyl Acetate Extracts

Disease cancer caused by abnormal growth of tissue where there has been an error, fast and out of control. Judging from the fact of gender, more than 270,000 women die every year caused by cervical cancer. To inhibit the growth of cancer cells, a compound is needed that causes the cell cycle to stop so that the ability of cell proliferation decreases. Alkaloid compounds can inhibit proliferation through oxidative inhibition processes that can cause cancer. Mangrove plants have potential as anticancer, antimicrobial, and antioxidant. The content of chemical compounds found in mangroves are flavonoids, steroids, alkaloids, phenolites, saponins and tannins. These compounds show high antioxidant activity and are shown to have a real relationship with the properties of the material's bioactivity against cancer cells. One of the mangrove species is Rhizophora apiculata. The purpose of this study was to determine the IC50 value produced by Rhizophora apiculata mangrove leaf extract on HeLa cell viability and to see the effect of Rhizophora apiculata mangrove leaf extract dosage on HeLa cell viability. The method used in this research is the experimental method. The research parameters included yield, proximate test, phytochemical test, toxicity test, total phenol test, cytotoxicity test and LC-MS test. The experimental design used was a simple and complex completely randomized design (CRD) with the Tukey test.The results of this study showed that the highest yield was in the ethanol extract of 5.91%, while the n-hexane and ethyl acetate extracts respectively had yields of 1.18% and 1.31%. The results of the proximate test on the water content of leaves and powder were 64.53% and 13.86%, respectively, the results of the ash content in the leaves and powder of Rhizophora apiculata were 3.94% and 8.41%, respectively. while the water content in the extract obtained the highest yield in the ethanol extract of 21.42%, while the n-hexane extract and ethyl acetate extract were 11.08% and 15.42%, respectively. For phytochemical results, it was found that n-hexane extract only contained alkaloids, flavonoids and steroids. Ethyl acetate extract contains steroid compounds. Meanwhile, the ethanol extract contains the most bioactive compounds, namely saponins, flavonoids, tannins and triterpenoids. The toxicity test using the Brine Shrimp Lethality Test (BSLT) method resulted in the lowest IC50 of ethanol extract at 49.45 ppm while the n-hexane and ethyl acetate extracts were 251.63 ppm and 920.45 ppm respectively. In the total phenol test, the n-hexane extract was 66.79 mg GAE / 100 gr, 222.97 mg GAE / 100 gr ethyl acetate extract and 929.04 mg GAE / 100 gr ethanol extract. HeLa cell cytotoxicity testing using the MTT method (3- (4,5-dimethiltiazol-2-yl) -2,5-dipheniltetra zolium bromide) assay resulted in the highest cell viability value at a dose of 125 ppm of 46.97%. As for the doses of 250 ppm, 500 ppm 1000 ppm, and 2000 ppm resulted in a percentage of viability of 42.95% 37.70% 35.82% and 32.12%, respectively. The IC50 value of Rhizophora apiculata leaf extract was 64.42 ppm. This value indicates that the Rhizophora apiculata extract is toxic to HeLa cells.

scholarly journals Uji Efektivitas Bioherbisida Ekstrak Etil Asetat Babadotan (Ageratum conyzoides L.) Dari Berbagai Ketinggian Tempat Dan Konsentrasi Terhadap Pertumbuhan Gulma Bayam Duri (Amaranthus spinosus L.)

2020 ◽  
Vol 3 (4) ◽  
pp. 34-42
Author(s):  
Zainuddin Zainuddin ◽  
Siti Hafsah ◽  
Gina Erida
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethyl Acetate Fraction ◽  
High Rate ◽  
Ageratum Conyzoides ◽  
Amaranthus Spinosus ◽  
Number Of Leaves ◽  
Materials Used ◽  
Banda Aceh ◽  
Ethyl Acetate Extracts

Abstrak. Penelitian ini bertujuan untuk menguji aktivitas ekstrak etil asetat babadotan dari berbagai ketinggian tempat dan konsentrasi terhadap pertumbuhan gulma bayam duri. Penelitian ini dilaksanakan di Laboratorium Ilmu Gulma Program Studi Agroteknologi dan Kebun Percobaan Fakultas Pertanian Universitas Syiah Kuala Banda Aceh, serta Laboratorium Analisis Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA), sejak September 2017– Januari 2018. Bahan yang digunakan adalah benih bayam duri, yang diperoleh dari Kecamatan Delima Kabupaten Pidie dan daun babadotan yang diperoleh dari 3 daerah dengan ketinggian yang berbeda yaitu Kecamatan Indrapuri Kabupaten Aceh Besar (19 mdpl), Kecamatan Lembah Seulawah Saree Kabupaten Aceh Besar (261 mdpl) dan Kecamatan Takengon Kabupaten Aceh Tengah (1259 mdpl) dengan konsentrasi 5, 10 dan 15% sebagai bioherbisida. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) pola faktorial 3  3 dengan 3 kali ulangan. Faktor pertama yaitu ketinggian tempat dengan 3 taraf dan faktor kedua konsentrasi dengan 3 taraf. Ekstrak yang dihasilkan pada ketinggian tempat yang berbeda dan konsentrasi ekstrak etil asetat berpengaruh nyata terhadap laju tinggi, jumlah daun, diameter batang, persentase pengendalian dan panjang akar. Terdapat interaksi antara ketinggian tempat dan konsentrasi terhadap persentase pengendalian pada 7 dan 14 HSA. Ekstrak yang dihasilkan pada ketinggian  9 mdpl dengan konsentrasi 10% dapat mengendalikan gulma bayam duri hingga 100% pada 7 HSA.The Test of Effectiveness Whiteweed Ethyl Acetate Extract (Ageratum conyzoides L.) from Various Altitudes And Concentration Against the Growth of Spiny Amaranth Weed (Amaranthus spinosus L.)Abstract. This study aims to examine the activity of ethyl acetate fraction of whiteweed extract from various altitudes and concentration againts the growth of spiny amaranth. This  research was conducted at Laboratory of Weed Science, Agrotechnology Department, and Experimental Garden, Agriculture Faculty, Syiah Kuala University Banda Aceh, and Laboratory of Chemical Analysis Mathematics Faculty and Natural Science, from September 2017 to January 2018. The materials used is spiny amaranth seed that obtained from Subdistrict of Delima, District of Pidie and babadotan leaves obtained from three regions with different altitude that is Subdistrict of Indrapuri, District of Aceh Besar (19 masl), Subdistrict of Subdistrict of, District of Aceh Besar (261 masl) and Subdistrict of Takengon District of Aceh Tengah (1259 masl) with each concentration 5, 10 and 15% as bioherbicide. This research using Randomized Completely Design with Factorial pattern which has 3 × 3 treatments and 3 replication. The first faktor is altitude and second is concentration of ethyl acetate extract. Ethyl acetate extracts that produced in different altitudes and concentrations were significantly influence the high rate of spiny amaranth, number of leaves, stem diameter, control percentage and root length. There is an interaction between altitude and concentration on the percentage of control at 7 and 14 HSA. Extracts produced at an altitude of 9 masl with a concentration of 10% can control spinach weeds up to 100% at 7 HSA.

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